JP5582576B2 - 活性の高いケトール酸レダクトイソメラーゼ酵素を用いたイソブタノールの発酵生成 - Google Patents
活性の高いケトール酸レダクトイソメラーゼ酵素を用いたイソブタノールの発酵生成 Download PDFInfo
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- JP5582576B2 JP5582576B2 JP2010504212A JP2010504212A JP5582576B2 JP 5582576 B2 JP5582576 B2 JP 5582576B2 JP 2010504212 A JP2010504212 A JP 2010504212A JP 2010504212 A JP2010504212 A JP 2010504212A JP 5582576 B2 JP5582576 B2 JP 5582576B2
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- isobutanol
- gene
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- seq
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/16—Butanols
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/42—Hydroxy-carboxylic acids
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Plant Pathology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US91249107P | 2007-04-18 | 2007-04-18 | |
| US60/912,491 | 2007-04-18 | ||
| PCT/US2008/060466 WO2008130995A2 (en) | 2007-04-18 | 2008-04-16 | Fermentive production of isobutanol using highly active ketol-acid reductoisomerase enzymes |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014107940A Division JP5908026B2 (ja) | 2007-04-18 | 2014-05-26 | 活性の高いケトール酸レダクトイソメラーゼ酵素を用いたイソブタノールの発酵生成 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2010524470A JP2010524470A (ja) | 2010-07-22 |
| JP2010524470A5 JP2010524470A5 (enExample) | 2011-05-06 |
| JP5582576B2 true JP5582576B2 (ja) | 2014-09-03 |
Family
ID=39872591
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010504212A Expired - Fee Related JP5582576B2 (ja) | 2007-04-18 | 2008-04-16 | 活性の高いケトール酸レダクトイソメラーゼ酵素を用いたイソブタノールの発酵生成 |
| JP2014107940A Active JP5908026B2 (ja) | 2007-04-18 | 2014-05-26 | 活性の高いケトール酸レダクトイソメラーゼ酵素を用いたイソブタノールの発酵生成 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014107940A Active JP5908026B2 (ja) | 2007-04-18 | 2014-05-26 | 活性の高いケトール酸レダクトイソメラーゼ酵素を用いたイソブタノールの発酵生成 |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US7910342B2 (enExample) |
| EP (2) | EP2543721A1 (enExample) |
| JP (2) | JP5582576B2 (enExample) |
| KR (1) | KR101573775B1 (enExample) |
| CN (1) | CN101680007A (enExample) |
| AU (1) | AU2008242978B2 (enExample) |
| BR (1) | BRPI0808600A2 (enExample) |
| CA (1) | CA2683898C (enExample) |
| ES (1) | ES2426070T3 (enExample) |
| MX (1) | MX2009011192A (enExample) |
| NZ (1) | NZ579780A (enExample) |
| WO (1) | WO2008130995A2 (enExample) |
| ZA (1) | ZA200906811B (enExample) |
Families Citing this family (128)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8273558B2 (en) | 2005-10-26 | 2012-09-25 | Butamax(Tm) Advanced Biofuels Llc | Fermentive production of four carbon alcohols |
| US9303225B2 (en) | 2005-10-26 | 2016-04-05 | Butamax Advanced Biofuels Llc | Method for the production of isobutanol by recombinant yeast |
| US8945899B2 (en) | 2007-12-20 | 2015-02-03 | Butamax Advanced Biofuels Llc | Ketol-acid reductoisomerase using NADH |
| US8956850B2 (en) | 2008-06-05 | 2015-02-17 | Butamax Advanced Biofuels Llc | Enhanced pyruvate to acetolactate conversion in yeast |
| WO2008098227A2 (en) * | 2007-02-09 | 2008-08-14 | The Regents Of The University Of California | Biofuel production by recombinant microorganisms |
| TWI568847B (zh) | 2007-03-16 | 2017-02-01 | 奇諾麥提卡公司 | 用於1,4-丁二醇及其前驅物之生物合成的組合物及方法 |
| WO2008124523A1 (en) * | 2007-04-04 | 2008-10-16 | The Regents Of The University Of California | Butanol production by recombinant microorganisms |
| WO2008130995A2 (en) | 2007-04-18 | 2008-10-30 | E. I. Du Pont De Nemours And Company | Fermentive production of isobutanol using highly active ketol-acid reductoisomerase enzymes |
| US7947483B2 (en) | 2007-08-10 | 2011-05-24 | Genomatica, Inc. | Methods and organisms for the growth-coupled production of 1,4-butanediol |
| CN101903530A (zh) * | 2007-10-12 | 2010-12-01 | 加利福尼亚大学董事会 | 被改造以产生异丙醇的微生物 |
| EP2225351A4 (en) | 2007-12-03 | 2016-11-09 | Gevo Inc | RENEWABLE COMPOSITIONS |
| US8193402B2 (en) | 2007-12-03 | 2012-06-05 | Gevo, Inc. | Renewable compositions |
| US8114641B2 (en) | 2007-12-10 | 2012-02-14 | Synthetic Genomics, Inc. | Methylbutanol as an advanced biofuel |
| EP3450550A1 (en) | 2008-03-05 | 2019-03-06 | Genomatica, Inc. | Primary alcohol producing organisms |
| PL2265709T3 (pl) | 2008-03-27 | 2018-05-30 | Genomatica, Inc. | Mikroorganizmy do produkcji kwasu adypinowego i innych związków |
| US8188250B2 (en) | 2008-04-28 | 2012-05-29 | Butamax(Tm) Advanced Biofuels Llc | Butanol dehydrogenase enzyme from the bacterium Achromobacter xylosoxidans |
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| CA2735883C (en) | 2008-09-10 | 2020-05-05 | Genomatica, Inc. | Microorganisms for the production of 1,4-butanediol |
| AU2009296224B2 (en) | 2008-09-29 | 2015-02-12 | Butamax(Tm) Advanced Biofuels Llc | Increased heterologous Fe-S enzyme actiivty in yeast |
| BRPI0913681A2 (pt) * | 2008-09-29 | 2019-09-24 | Butamax Advanced Biofuels Llc | "célula bacteriana ácido-lática recombinante e método de produção de isobutanol" |
| US20100081154A1 (en) * | 2008-09-29 | 2010-04-01 | Butamax(Tm) Advanced Biofuels Llc | IDENTIFICATION AND USE OF BACTERIAL [2Fe-2S] DIHYDROXY-ACID DEHYDRATASES |
| WO2010051527A2 (en) * | 2008-10-31 | 2010-05-06 | Gevo, Inc. | Engineered microorganisms capable of producing target compounds under anaerobic conditions |
| US8465964B2 (en) | 2008-11-13 | 2013-06-18 | Butamax (TM) Advanced Biofules LLC | Increased production of isobutanol in yeast with reduced mitochondrial amino acid biosynthesis |
| US8828694B2 (en) * | 2008-11-13 | 2014-09-09 | Butamax Advanced Biofuels Llc | Production of isobutanol in yeast mitochondria |
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| US8795992B2 (en) * | 2008-12-29 | 2014-08-05 | Butamax Advanced Biofuels Llc | Yeast with increased butanol tolerance involving cell wall integrity pathway |
| US8557562B2 (en) * | 2008-12-29 | 2013-10-15 | Butamax(Tm) Advanced Biofuels Llc | Yeast with increased butanol tolerance involving filamentous growth response |
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| BR112012006939A2 (pt) * | 2009-09-29 | 2015-09-08 | Butamax Tm Advanced Biofuels | célula hospedeira de produção de levedura recombinante e método para a produção de um produto selecionado do grupo que consiste em 2,3-butanodiol, isobutanol, 2-butanol, 1-butanol, 2-butanona, valina, leucina, ácido lático, ácido málico, álcool, isoampilico e isoprenoides |
| JP5805094B2 (ja) * | 2009-09-29 | 2015-11-04 | ビュータマックス・アドバンスド・バイオフューエルズ・エルエルシー | 高有効性のケトール酸レダクトイソメラーゼ酵素を用いたイソブタノールの発酵生産 |
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| WO2008130995A3 (en) | 2009-02-05 |
| AU2008242978A1 (en) | 2008-10-30 |
| CA2683898C (en) | 2016-02-02 |
| EP2134849B1 (en) | 2013-07-10 |
| BRPI0808600A2 (pt) | 2018-12-26 |
| EP2134849A2 (en) | 2009-12-23 |
| KR101573775B1 (ko) | 2015-12-04 |
| JP2010524470A (ja) | 2010-07-22 |
| AU2008242978B2 (en) | 2014-09-18 |
| US20080261230A1 (en) | 2008-10-23 |
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