JP5529322B2 - トランスジェニック事象mon89034に対応するトウモロコシ植物および種子ならびにそれらを検出および使用するための方法 - Google Patents
トランスジェニック事象mon89034に対応するトウモロコシ植物および種子ならびにそれらを検出および使用するための方法 Download PDFInfo
- Publication number
- JP5529322B2 JP5529322B2 JP2013088193A JP2013088193A JP5529322B2 JP 5529322 B2 JP5529322 B2 JP 5529322B2 JP 2013088193 A JP2013088193 A JP 2013088193A JP 2013088193 A JP2013088193 A JP 2013088193A JP 5529322 B2 JP5529322 B2 JP 5529322B2
- Authority
- JP
- Japan
- Prior art keywords
- dna
- seq
- corn
- dna molecule
- mon89034
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000000034 method Methods 0.000 title claims description 84
- 241001057636 Dracaena deremensis Species 0.000 title claims description 67
- 230000009261 transgenic effect Effects 0.000 title description 93
- 108020004414 DNA Proteins 0.000 claims description 299
- 240000008042 Zea mays Species 0.000 claims description 210
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 200
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 179
- 235000005822 corn Nutrition 0.000 claims description 179
- 239000000523 sample Substances 0.000 claims description 125
- 125000003729 nucleotide group Chemical group 0.000 claims description 87
- 239000002773 nucleotide Substances 0.000 claims description 86
- 239000013615 primer Substances 0.000 claims description 86
- 108091093088 Amplicon Proteins 0.000 claims description 85
- 241000196324 Embryophyta Species 0.000 claims description 60
- 230000000295 complement effect Effects 0.000 claims description 54
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 51
- 102000053602 DNA Human genes 0.000 claims description 51
- 150000007523 nucleic acids Chemical class 0.000 claims description 50
- 239000012472 biological sample Substances 0.000 claims description 47
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 44
- 102000039446 nucleic acids Human genes 0.000 claims description 41
- 108020004707 nucleic acids Proteins 0.000 claims description 41
- 230000003321 amplification Effects 0.000 claims description 40
- 238000009396 hybridization Methods 0.000 claims description 34
- 108700019146 Transgenes Proteins 0.000 claims description 33
- 238000006243 chemical reaction Methods 0.000 claims description 32
- 238000001514 detection method Methods 0.000 claims description 28
- 238000003745 diagnosis Methods 0.000 claims description 25
- 108020001019 DNA Primers Proteins 0.000 claims description 20
- 239000003155 DNA primer Substances 0.000 claims description 20
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims description 20
- 235000009973 maize Nutrition 0.000 claims description 20
- 230000004544 DNA amplification Effects 0.000 claims description 15
- 235000005687 corn oil Nutrition 0.000 claims description 8
- 239000002285 corn oil Substances 0.000 claims description 8
- 235000013312 flour Nutrition 0.000 claims description 6
- 230000002441 reversible effect Effects 0.000 claims description 5
- 229920002261 Corn starch Polymers 0.000 claims description 4
- 101710089042 Demethyl-4-deoxygadusol synthase Proteins 0.000 claims description 4
- 239000008120 corn starch Substances 0.000 claims description 4
- 235000012054 meals Nutrition 0.000 claims description 4
- GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical compound CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 claims description 2
- WQZGKKKJIJFFOK-DVKNGEFBSA-N alpha-D-glucose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-DVKNGEFBSA-N 0.000 claims description 2
- 239000004067 bulking agent Substances 0.000 claims description 2
- 239000002537 cosmetic Substances 0.000 claims description 2
- 235000013310 margarine Nutrition 0.000 claims description 2
- 239000003264 margarine Substances 0.000 claims description 2
- 235000020357 syrup Nutrition 0.000 claims description 2
- 239000006188 syrup Substances 0.000 claims description 2
- 108020003215 DNA Probes Proteins 0.000 claims 4
- 239000003298 DNA probe Substances 0.000 claims 4
- 108020005120 Plant DNA Proteins 0.000 claims 1
- 230000014509 gene expression Effects 0.000 description 33
- 210000004027 cell Anatomy 0.000 description 32
- 108091028043 Nucleic acid sequence Proteins 0.000 description 31
- 102000040430 polynucleotide Human genes 0.000 description 26
- 108091033319 polynucleotide Proteins 0.000 description 26
- 239000002157 polynucleotide Substances 0.000 description 26
- 108090000623 proteins and genes Proteins 0.000 description 26
- 238000003752 polymerase chain reaction Methods 0.000 description 24
- 241000238631 Hexapoda Species 0.000 description 23
- 239000000047 product Substances 0.000 description 19
- 230000027455 binding Effects 0.000 description 15
- 241000607479 Yersinia pestis Species 0.000 description 11
- 238000003780 insertion Methods 0.000 description 11
- 230000037431 insertion Effects 0.000 description 11
- 235000018102 proteins Nutrition 0.000 description 11
- 102000004169 proteins and genes Human genes 0.000 description 11
- 230000002068 genetic effect Effects 0.000 description 10
- 230000000749 insecticidal effect Effects 0.000 description 10
- 238000004458 analytical method Methods 0.000 description 9
- 239000000203 mixture Substances 0.000 description 9
- 108700012359 toxins Proteins 0.000 description 8
- 108091026890 Coding region Proteins 0.000 description 7
- 108091034117 Oligonucleotide Proteins 0.000 description 7
- 238000002866 fluorescence resonance energy transfer Methods 0.000 description 7
- 239000002987 primer (paints) Substances 0.000 description 7
- 239000003053 toxin Substances 0.000 description 7
- 231100000765 toxin Toxicity 0.000 description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- 238000003556 assay Methods 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 235000013305 food Nutrition 0.000 description 6
- 239000012634 fragment Substances 0.000 description 6
- 230000009545 invasion Effects 0.000 description 6
- 210000001519 tissue Anatomy 0.000 description 6
- 241000255777 Lepidoptera Species 0.000 description 5
- 241000209140 Triticum Species 0.000 description 5
- 235000021307 Triticum Nutrition 0.000 description 5
- 239000002917 insecticide Substances 0.000 description 5
- 239000003550 marker Substances 0.000 description 5
- 230000013011 mating Effects 0.000 description 5
- 239000013612 plasmid Substances 0.000 description 5
- 231100000331 toxic Toxicity 0.000 description 5
- 230000002588 toxic effect Effects 0.000 description 5
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 4
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 4
- 108700028369 Alleles Proteins 0.000 description 4
- 230000004568 DNA-binding Effects 0.000 description 4
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 4
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 4
- 238000009395 breeding Methods 0.000 description 4
- 239000013642 negative control Substances 0.000 description 4
- 230000008707 rearrangement Effects 0.000 description 4
- 230000001105 regulatory effect Effects 0.000 description 4
- 150000003839 salts Chemical class 0.000 description 4
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 3
- 240000007594 Oryza sativa Species 0.000 description 3
- 235000007164 Oryza sativa Nutrition 0.000 description 3
- 235000007244 Zea mays Nutrition 0.000 description 3
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 3
- 230000009418 agronomic effect Effects 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000021615 conjugation Effects 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 235000005911 diet Nutrition 0.000 description 3
- 230000037213 diet Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000007899 nucleic acid hybridization Methods 0.000 description 3
- 239000000575 pesticide Substances 0.000 description 3
- 230000008488 polyadenylation Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000010791 quenching Methods 0.000 description 3
- 230000000171 quenching effect Effects 0.000 description 3
- 235000009566 rice Nutrition 0.000 description 3
- 102000007469 Actins Human genes 0.000 description 2
- 108010085238 Actins Proteins 0.000 description 2
- 241000566547 Agrotis ipsilon Species 0.000 description 2
- 241000255967 Helicoverpa zea Species 0.000 description 2
- 206010061217 Infestation Diseases 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 2
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 2
- 241001147398 Ostrinia nubilalis Species 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 241000256251 Spodoptera frugiperda Species 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 239000006227 byproduct Substances 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 2
- 230000002759 chromosomal effect Effects 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 230000001351 cycling effect Effects 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 210000005069 ears Anatomy 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 239000007850 fluorescent dye Substances 0.000 description 2
- 239000004009 herbicide Substances 0.000 description 2
- 230000010354 integration Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000002853 nucleic acid probe Substances 0.000 description 2
- 239000002751 oligonucleotide probe Substances 0.000 description 2
- 230000010287 polarization Effects 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 229920002477 rna polymer Polymers 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 230000008685 targeting Effects 0.000 description 2
- 231100000167 toxic agent Toxicity 0.000 description 2
- 239000003440 toxic substance Substances 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- 230000001131 transforming effect Effects 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- IAJOBQBIJHVGMQ-UHFFFAOYSA-N 2-amino-4-[hydroxy(methyl)phosphoryl]butanoic acid Chemical compound CP(O)(=O)CCC(N)C(O)=O IAJOBQBIJHVGMQ-UHFFFAOYSA-N 0.000 description 1
- 108020005065 3' Flanking Region Proteins 0.000 description 1
- 108020005029 5' Flanking Region Proteins 0.000 description 1
- 241000589158 Agrobacterium Species 0.000 description 1
- 102000007347 Apyrase Human genes 0.000 description 1
- 108010007730 Apyrase Proteins 0.000 description 1
- 235000007319 Avena orientalis Nutrition 0.000 description 1
- 241000209763 Avena sativa Species 0.000 description 1
- 235000007558 Avena sp Nutrition 0.000 description 1
- 108700003918 Bacillus Thuringiensis insecticidal crystal Proteins 0.000 description 1
- 241000193388 Bacillus thuringiensis Species 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 102000014914 Carrier Proteins Human genes 0.000 description 1
- 101710163595 Chaperone protein DnaK Proteins 0.000 description 1
- 108010077544 Chromatin Proteins 0.000 description 1
- 241000254173 Coleoptera Species 0.000 description 1
- IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical compound OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 description 1
- 238000001712 DNA sequencing Methods 0.000 description 1
- CYCGRDQQIOGCKX-UHFFFAOYSA-N Dehydro-luciferin Natural products OC(=O)C1=CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 CYCGRDQQIOGCKX-UHFFFAOYSA-N 0.000 description 1
- 241000879145 Diatraea grandiosella Species 0.000 description 1
- 101100125027 Dictyostelium discoideum mhsp70 gene Proteins 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 244000148064 Enicostema verticillatum Species 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108700039887 Essential Genes Proteins 0.000 description 1
- BJGNCJDXODQBOB-UHFFFAOYSA-N Fivefly Luciferin Natural products OC(=O)C1CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 BJGNCJDXODQBOB-UHFFFAOYSA-N 0.000 description 1
- 239000005561 Glufosinate Substances 0.000 description 1
- 108010068370 Glutens Proteins 0.000 description 1
- 239000005562 Glyphosate Substances 0.000 description 1
- 101150031823 HSP70 gene Proteins 0.000 description 1
- 101710178376 Heat shock 70 kDa protein Proteins 0.000 description 1
- 101710152018 Heat shock cognate 70 kDa protein Proteins 0.000 description 1
- 244000020551 Helianthus annuus Species 0.000 description 1
- 235000003222 Helianthus annuus Nutrition 0.000 description 1
- 108010034791 Heterochromatin Proteins 0.000 description 1
- 108060001084 Luciferase Proteins 0.000 description 1
- 239000005089 Luciferase Substances 0.000 description 1
- DDWFXDSYGUXRAY-UHFFFAOYSA-N Luciferin Natural products CCc1c(C)c(CC2NC(=O)C(=C2C=C)C)[nH]c1Cc3[nH]c4C(=C5/NC(CC(=O)O)C(C)C5CC(=O)O)CC(=O)c4c3C DDWFXDSYGUXRAY-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 108091092878 Microsatellite Proteins 0.000 description 1
- 241000346285 Ostrinia furnacalis Species 0.000 description 1
- 101710091688 Patatin Proteins 0.000 description 1
- 108010010677 Phosphodiesterase I Proteins 0.000 description 1
- 241000178953 Photorhabdus sp. Species 0.000 description 1
- 108010089814 Plant Lectins Proteins 0.000 description 1
- 239000004952 Polyamide Substances 0.000 description 1
- 108091030071 RNAI Proteins 0.000 description 1
- 244000081426 Ranunculus ficaria Species 0.000 description 1
- 235000002226 Ranunculus ficaria Nutrition 0.000 description 1
- 108010003581 Ribulose-bisphosphate carboxylase Proteins 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- 240000006394 Sorghum bicolor Species 0.000 description 1
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 1
- 244000062793 Sorghum vulgare Species 0.000 description 1
- 102000004523 Sulfate Adenylyltransferase Human genes 0.000 description 1
- 108010022348 Sulfate adenylyltransferase Proteins 0.000 description 1
- 108010006785 Taq Polymerase Proteins 0.000 description 1
- 235000009430 Thespesia populnea Nutrition 0.000 description 1
- 108091036066 Three prime untranslated region Proteins 0.000 description 1
- 239000007984 Tris EDTA buffer Substances 0.000 description 1
- 241000500606 Xenorhabdus sp. Species 0.000 description 1
- 101100232046 Zea mays HSP70 gene Proteins 0.000 description 1
- 241000482268 Zea mays subsp. mays Species 0.000 description 1
- IRLPACMLTUPBCL-FCIPNVEPSA-N adenosine-5'-phosphosulfate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@@H](CO[P@](O)(=O)OS(O)(=O)=O)[C@H](O)[C@H]1O IRLPACMLTUPBCL-FCIPNVEPSA-N 0.000 description 1
- 235000013334 alcoholic beverage Nutrition 0.000 description 1
- 230000000735 allogeneic effect Effects 0.000 description 1
- 108091008324 binding proteins Proteins 0.000 description 1
- 230000000443 biocontrol Effects 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 229930002868 chlorophyll a Natural products 0.000 description 1
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
- 229930002869 chlorophyll b Natural products 0.000 description 1
- NSMUHPMZFPKNMZ-VBYMZDBQSA-M chlorophyll b Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C=O)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 NSMUHPMZFPKNMZ-VBYMZDBQSA-M 0.000 description 1
- 210000003763 chloroplast Anatomy 0.000 description 1
- 108010031100 chloroplast transit peptides Proteins 0.000 description 1
- 210000003483 chromatin Anatomy 0.000 description 1
- 239000013611 chromosomal DNA Substances 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 230000004186 co-expression Effects 0.000 description 1
- 238000004590 computer program Methods 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 244000038559 crop plants Species 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000000539 dimer Substances 0.000 description 1
- 101150052825 dnaK gene Proteins 0.000 description 1
- 230000024346 drought recovery Effects 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000002158 endotoxin Substances 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 239000011536 extraction buffer Substances 0.000 description 1
- 238000002875 fluorescence polarization Methods 0.000 description 1
- 235000012041 food component Nutrition 0.000 description 1
- 239000005417 food ingredient Substances 0.000 description 1
- 230000009368 gene silencing by RNA Effects 0.000 description 1
- 235000021312 gluten Nutrition 0.000 description 1
- XDDAORKBJWWYJS-UHFFFAOYSA-N glyphosate Chemical compound OC(=O)CNCP(O)(O)=O XDDAORKBJWWYJS-UHFFFAOYSA-N 0.000 description 1
- 229940097068 glyphosate Drugs 0.000 description 1
- 210000004458 heterochromatin Anatomy 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 235000021374 legumes Nutrition 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 235000019713 millet Nutrition 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 239000003068 molecular probe Substances 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 239000002102 nanobead Substances 0.000 description 1
- 239000002071 nanotube Substances 0.000 description 1
- 108010058731 nopaline synthase Proteins 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 239000003973 paint Substances 0.000 description 1
- 238000003976 plant breeding Methods 0.000 description 1
- 239000003726 plant lectin Substances 0.000 description 1
- 229920002647 polyamide Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000012175 pyrosequencing Methods 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 230000000306 recurrent effect Effects 0.000 description 1
- 235000021067 refined food Nutrition 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 238000011426 transformation method Methods 0.000 description 1
- 241001515965 unidentified phage Species 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
- A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
- A01H6/4684—Zea mays [maize]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
- A01H5/10—Seeds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
- C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Botany (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Mycology (AREA)
- Cell Biology (AREA)
- Pest Control & Pesticides (AREA)
- Insects & Arthropods (AREA)
- Plant Pathology (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Physiology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Nutrition Science (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Description
本願は、2006年5月26日に出願された米国仮出願番号60/808,834に対して優先権の利益を主張するものである。
本発明は、トランスジェニックトウモロコシ事象(event)MON89034ならびにその植物体の一部および種子に関する。この事象は、鱗翅(Lepidoptera)目の昆虫による昆虫侵入に抵抗性を示す。本発明はまた、このトランスジェニック事象に特有のヌクレオチド配列の存在について探索するときにこのトランスジェニック事象の存在についての診断であるDNAを含む植物および種子を使用するための方法、ならびに、このトランスジェニック事象に特有の特異的なヌクレオチド配列を検出することによって生物学的サンプル中の前記トウモロコシ事象の存在を検出するための方法にも関する。本発明は、この事象に特有のヌクレオチド配列を提供する。
本発明は、本明細書中で事象MON89034と呼ばれるトウモロコシ(Zea mays)植物の鱗翅目(Lepidopteran)抵抗性トランスジェニック品種に関し、また、トウモロコシの任意のサンプルまたは品種において検出されるときに、そのサンプルまたは品種中に存在する、トランスジェニックトウモロコシ植物事象MON89034の存在についての診断である特有のDNA配列に関し、そして、トウモロコシMON89034ならびにそれから得られる子孫植物および種子における導入遺伝子/ゲノム挿入領域の検出にも関する。
特許文献2:米国特許第6,620,988号明細書
非特許文献2:Roush(1994)Biocontrol. Sci. Technol.. 4:501-516
非特許文献3:Weising et al. (19880 Ann. Rev. Genet 22:421-477
本発明は、American Type Culture Collection(ATCC)に受入番号PTA-7455として2006年3月28日に寄託された種子を有する、MON89034と命名されたトランスジェニックトウモロコシ植物およびその(挿入されたトランスジェニックDNAに相当する少なくとも1つの対立遺伝子も含む限り)トウモロコシ事象MON89034と識別不能な子孫に関する。本発明の別の態様は、配列番号:1、配列番号:2、配列番号:3、配列番号:4および配列番号:5よりなる群から選択されるポリヌクレオチドを含む、子孫植物もしくは種子またはトウモロコシ事象MON89034の植物および種子の再生可能な一部である。本発明はまた、トウモロコシ事象MON89034の植物体の一部分を含み、その植物体の一部分が、上で示したようなポリヌクレオチドを少なくとも含む限り、これらの一部分として、花粉、胚珠、花、苗条、根、柄、花柱、雄穂、穂および葉が挙げられるが、これらに限定されない。MON89034事象に対応するトウモロコシ植物の分野におけるMON89034のゲノム内に含まれる新規遺伝的組成物およびMON89034からの製品(例えば、粗挽き粉、小麦粉、油、パルプおよび残渣バイオマス(biomass left over))は、本発明の一態様である。
以下の定義および方法は、本発明をよりよく定義するため、および、本発明を実施する際に当業者を導くために提供される。別段述べられない限り、用語は、関連分野の当業者による慣例的用法に従って理解されるべきである。分子生物学における一般的な用語の定義は、Rieger et al., Glossary of Genetics:Classical and Molecular,5th edition, Springer-Verlag:New York,1991;およびLewin, Genes V, Oxford University Press:New York,1994内に見られることがある。
(a)配列番号:5に示されるようなヌクレオチド配列;
(b)配列番号:1および配列番号:2に示されるようなヌクレオチド配列の両方;
(c)配列番号:3に示されるようなヌクレオチド配列;および
(d)配列番号:4に示されるようなヌクレオチド配列
よりなる群から選択される配列のうちのいずれか1つまたはすべてを含む。
この実施例では、トランスジェニックトウモロコシ事象MON89034の構築および分子の特徴づけを説明する。
この実施例では、ゲノム内のトランスジェニックトウモロコシ事象MON89034についての診断であるDNAを含むトウモロコシ植物の同定およびそのようなトウモロコシ植物の接合状態の判定を説明する。
Claims (21)
- 第1のDNA分子および第2のDNA分子を含むDNA分子対であって、いずれのDNA分子も、配列番号:3もしくは配列番号:5またはその相補体の十分な長さの連続的なヌクレオチドであって、トウモロコシ植物MON89034またはその子孫から抽出されたDNAに特異的なDNAプライマーまたはプローブとして機能し、ここに、前記第1のDNA分子および第2のDNA分子は、DNA増幅法に共に使用されるとき、配列番号:1またはその相補体を含むアンプリコンを生成する、DNA分子対。
- 第1のDNA分子が、配列番号:3もしくは配列番号:5またはその相補体の導入遺伝子領域の任意の部分の連続的な11以上のヌクレオチドを含み、第2のDNA分子が、配列番号:3またはその相補体の5’フランキングトウモロコシゲノムDNA領域の任意の部分の連続的な11以上のヌクレオチドを含む、請求項1に記載のDNA分子対。
- 第1のDNA分子が、配列番号:5の異種挿入DNA部分に対して完全に相補的な連続的な少なくとも15ヌクレオチドを含み、第2のDNA分子が、配列番号:3のトウモロコシゲノム部分からの連続的な少なくとも15ヌクレオチドを含む、請求項2に記載のDNA分子対。
- 第1のDNA分子が、配列番号:5のヌクレオチド2060位からヌクレオチド12,208位の範囲内に特異的にハイブリダイズし、第2のDNA分子が、配列番号:3のヌクレオチド1位からヌクレオチド2050位の逆相補配列の範囲内に特異的にハイブリダイズする、請求項2に記載のDNA分子対。
- 第1のDNA分子および第2のDNA分子を含むDNA分子対であって、いずれのDNA分子も、配列番号:4もしくは配列番号:5またはその相補体の十分な長さの連続的なヌクレオチドであって、トウモロコシ植物MON89034またはその子孫から抽出されたDNAに特異的なDNAプライマーまたはプローブとして機能し、前記第1のDNA分子および第2のDNA分子は、DNA増幅法に共に使用されるとき、配列番号:2またはその相補体を含むアンプリコンを生成する、DNA分子対。
- 第1のDNA分子が、配列番号:4もしくは配列番号:5またはその相補体の導入遺伝子領域の任意の部分の連続的な11以上のヌクレオチドを含み、第2のDNA分子が、配列番号:4またはその相補体の3’フランキングトウモロコシゲノムDNA領域の任意の部分の連続的な11以上のヌクレオチドを含む、請求項5に記載のDNA分子対。
- 第1のDNA分子が、配列番号:5の異種挿入DNA部分からの連続的な少なくとも15ヌクレオチドを含み、第2のDNA分子が、配列番号:4のトウモロコシゲノム部分に対して完全に相補的な連続的な少なくとも15ヌクレオチドを含む、請求項6に記載のDNA分子対。
- 第1のDNA分子が、配列番号:5のヌクレオチド1位からヌクレオチド11305位の逆相補配列の範囲内に特異的にハイブリダイズし、第2のDNA分子が、配列番号:4のヌクレオチド21位からヌクレオチド914位の範囲内に特異的にハイブリダイズする、請求項6に記載のDNA分子対。
- 第1のDNA分子が配列番号:6を含み、第2のDNA分子が配列番号:7を含む、請求項1から8いずれかに記載のDNA分子対。
- 生物学的サンプル中の配列番号:3、配列番号:4および配列番号:5よりなる群から選択されるDNA分子の存在を検出する方法であって、
(a)生物学的サンプルを、配列番号:3もしくはその相補体、配列番号:4もしくはその相補体または配列番号:5もしくはその相補体の十分な長さの連続的なヌクレオチドであって、トウモロコシ植物MON89034またはその子孫から抽出されたDNAに特異的なDNAプライマーまたはプローブとして機能するDNA分子を含むDNAプライマー対と接触させ、ここに、前記DNAプライマー対は、DNA増幅法に共に使用されるとき、配列番号:1、配列番号:2またはその相補体を含むアンプリコンを生成し;
(b)核酸増幅反応条件に供し;
(c)核酸増幅反応を行って、DNAアンプリコン分子を生成し;次いで、
(d)前記DNAアンプリコン分子を検出することを含み、ここに、配列番号:1、配列番号:2およびそれらの相補体の少なくとも1つを含むアンプリコンの検出が、前記生物学的サンプル中の前記DNA分子の存在を示唆するものである方法。 - 前記生物学的サンプルがトウモロコシ植物から抽出されたDNAサンプルである、請求項10に記載の方法。
- 生物学的サンプル中の配列番号:3、配列番号:4および配列番号:5よりなる群から選択されるDNA分子の存在を検出する方法であって、
(a)生物学的サンプルを、高ストリンジェントな条件下において前記DNA分子とハイブリダイズし、かつ、前記高ストリンジェントな条件下において前記DNA分子を含有しない生物学的サンプルとはハイブリダイズしないDNAプローブと接触させ;
(b)前記生物学的サンプルおよびDNAプローブを高ストリンジェントなハイブリダイゼーション条件に供し;次いで、
(c)前記生物学的サンプルへの前記DNAプローブのハイブリダイゼーションを検出することを含み、ここに、ハイブリダイゼーションの検出が、前記生物学的サンプル中のDNA分子の存在を示唆するものであり、前記生物学的サンプルがトウモロコシ植物から抽出されたDNAサンプルである、方法。 - 前記DNAプローブが配列番号:1もしくは配列番号:2またはその相補体を含む、請求項12に記載の方法。
- 前記DNAプローブが少なくとも1つのフルオロフォアで標識されている、請求項12に記載の方法。
- 前記生物学的サンプルが、コーンミール、コーン油、コーンケーキ、トウモロコシ種子、トウモロコシ胚芽、コーンスターチ、トウモロコシ粉、トウモロコシ花粉、トウモロコシ花柱、コーン浸出液、コーンモルト、コーンシュガー、コーンシロップ、コーン油から生産されるマーガリン、乾燥蒸留粕(DDGS)、化粧品およびバルキング剤よりなる群から選択される、請求項10から14いずれかに記載の方法。
- 配列番号:3、配列番号:4、配列番号:5またはそれらの相補体の十分な長さの連続的なヌクレオチドであって、トウモロコシ事象MON89034および/またはその子孫に特異的なDNAプライマーまたはプローブとして機能する少なくとも1つのDNA分子を含む、DNA検出キット。
- 前記少なくとも1つのDNA分子が、配列番号:1、配列番号:2またはその相補体を含む、請求項16に記載のDNA検出キット。
- 前記少なくとも1つのDNA分子が、配列番号:1、配列番号:2またはその相補体を含む、請求項17に記載のDNA検出キット。
- 請求項1から9いずれかに記載のDNA分子対を含むDNA検出キット。
- 生物学的サンプル中のトウモロコシ事象MON89034を含むトウモロコシ植物のDNAの接合状態を決定する方法であって、
(a)前記サンプルを、(1)トウモロコシ事象MON89034 DNAを含む核酸増幅反応に用いるとき、トウモロコシ事象MON89034に対する診断である第1のアンプリコンを生成し、(2)トウモロコシ事象MON89034 DNA以外のトウモロコシゲノムDNAを含む核酸増幅反応に用いるとき、トウモロコシ事象MON89034 DNA以外のトウモロコシゲノムDNAに対する診断である第2のアンプリコンを生成する、配列番号:6、配列番号:7および配列番号:10を含むプライマーセットと接触させ;
(b)核酸増幅反応を行い;次いで、
(c)そのようにして生成されたアンプリコンを検出することを含み、ここに、両方のアンプリコンの存在の検出は、前記サンプルがトウモロコシ事象MON89034 DNAに対してヘテロ接合性であることを示唆するものであって、第1のアンプリコンのみの検出は、前記サンプルがトウモロコシ事象MON89034 DNAに対してホモ接合性であることを示唆するものである、方法。 - 前記プライマーセットが、さらに配列番号:14および配列番号:15を共に使用する、請求項20に記載の方法。
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US80883406P | 2006-05-26 | 2006-05-26 | |
US60/808,834 | 2006-05-26 |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2009513394A Division JP5513883B2 (ja) | 2006-05-26 | 2007-05-24 | トランスジェニック事象mon89034に対応するトウモロコシ植物および種子ならびにそれらを検出および使用するための方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2013150632A JP2013150632A (ja) | 2013-08-08 |
JP5529322B2 true JP5529322B2 (ja) | 2014-06-25 |
Family
ID=38779000
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2009513394A Active JP5513883B2 (ja) | 2006-05-26 | 2007-05-24 | トランスジェニック事象mon89034に対応するトウモロコシ植物および種子ならびにそれらを検出および使用するための方法 |
JP2013088193A Active JP5529322B2 (ja) | 2006-05-26 | 2013-04-19 | トランスジェニック事象mon89034に対応するトウモロコシ植物および種子ならびにそれらを検出および使用するための方法 |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2009513394A Active JP5513883B2 (ja) | 2006-05-26 | 2007-05-24 | トランスジェニック事象mon89034に対応するトウモロコシ植物および種子ならびにそれらを検出および使用するための方法 |
Country Status (33)
Country | Link |
---|---|
US (4) | US8062840B2 (ja) |
EP (1) | EP2021476B1 (ja) |
JP (2) | JP5513883B2 (ja) |
KR (2) | KR101366363B1 (ja) |
CN (2) | CN107119128B (ja) |
AP (1) | AP3797A (ja) |
AR (2) | AR061131A1 (ja) |
AU (1) | AU2007267586B2 (ja) |
BR (2) | BRPI0712921B1 (ja) |
CA (1) | CA2653338C (ja) |
CL (1) | CL2017002157A1 (ja) |
CR (1) | CR10461A (ja) |
DO (1) | DOP2007000106A (ja) |
EA (1) | EA022829B1 (ja) |
EG (1) | EG26119A (ja) |
ES (1) | ES2498976T3 (ja) |
HR (1) | HRP20140951T1 (ja) |
IL (1) | IL195451A (ja) |
MX (1) | MX2008015108A (ja) |
MY (1) | MY150655A (ja) |
NI (1) | NI200800312A (ja) |
NZ (1) | NZ573179A (ja) |
PE (1) | PE20080456A1 (ja) |
PL (1) | PL2021476T3 (ja) |
PT (1) | PT2021476E (ja) |
RS (1) | RS53560B1 (ja) |
SG (1) | SG171612A1 (ja) |
SI (1) | SI2021476T1 (ja) |
TW (1) | TWI453412B (ja) |
UA (1) | UA98770C2 (ja) |
UY (2) | UY30370A1 (ja) |
WO (1) | WO2007140256A1 (ja) |
ZA (1) | ZA200810027B (ja) |
Families Citing this family (332)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EG26529A (en) * | 2001-06-11 | 2014-01-27 | مونسانتو تكنولوجى ل ل سى | Prefixes for detection of DNA molecule in cotton plant MON15985 which gives resistance to damage caused by insect of squamous lepidoptera |
BRPI0712921B1 (pt) | 2006-05-26 | 2024-01-30 | Monsanto Technology Llc | Moléculas de dna do evento transgênico mon89034, métodos para detecção do referido evento, produção de plantas transgênicas compreendendo o mesmo, determinar sua zigosi5 dade, proteger uma planta de milho da infestação de insetos, bem como par de moléculas de dna e kit de detecção de dna |
US8716001B2 (en) | 2009-02-06 | 2014-05-06 | Cornell University | Trichoderma strains that induce resistance to plant diseases and/or increase plant growth |
ES2866126T3 (es) * | 2009-09-17 | 2021-10-19 | Monsanto Technology Llc | Evento transgénico de soja MON 87708 y procedimientos de uso del mismo |
MX358629B (es) | 2009-11-23 | 2018-08-28 | Monsanto Technology Llc | Evento transgenico de maiz mon 87427 y la escala de desarrollo relativo. |
BR112012015690A2 (pt) | 2009-12-23 | 2015-08-25 | Bayer Intelectual Property Gmbh | Plantas tolerantes a herbicidas inibidores de hppd. |
AR079882A1 (es) | 2009-12-23 | 2012-02-29 | Bayer Cropscience Ag | Plantas tolerantes a herbicidas inhibidores de las hppd |
ES2659085T3 (es) | 2009-12-23 | 2018-03-13 | Bayer Intellectual Property Gmbh | Plantas tolerantes a herbicidas inhibidores de HPPD |
CA2785208A1 (en) | 2009-12-23 | 2011-06-30 | Bayer Intellectual Property Gmbh | Plants tolerant to hppd inhibitor herbicides |
BR112012015692A2 (pt) | 2009-12-23 | 2015-08-25 | Bayer Intelectual Property Gmbh | Plantas tolerantes a herbicida inibidor de hppds. |
RS55986B1 (sr) | 2010-01-22 | 2017-09-29 | Bayer Ip Gmbh | Akaricidne i/ili insekticidne kombinacije aktivnih supstanci |
EP2669369A1 (en) | 2010-11-10 | 2013-12-04 | Bayer CropScience AG | HPPD variants and methods of use |
MX2013005643A (es) | 2010-11-29 | 2013-07-03 | Bayer Ip Gmbh | Iminas alfa, beta-insaturadas. |
CN103281900A (zh) | 2010-12-01 | 2013-09-04 | 拜耳知识产权有限责任公司 | 氟吡菌酰胺用于防治作物中的线虫以及提高产量的用途 |
EP2460407A1 (de) | 2010-12-01 | 2012-06-06 | Bayer CropScience AG | Wirkstoffkombinationen umfassend Pyridylethylbenzamide und weitere Wirkstoffe |
TWI667347B (zh) * | 2010-12-15 | 2019-08-01 | 瑞士商先正達合夥公司 | 大豆品種syht0h2及偵測其之組合物及方法 |
US20130345058A1 (en) | 2011-03-10 | 2013-12-26 | Wolfram Andersch | Use of lipochito-oligosaccharide compounds for safeguarding seed safety of treated seeds |
IN2013CN07575A (ja) | 2011-03-23 | 2015-07-31 | Bayer Ip Gmbh | |
CA2830802A1 (en) | 2011-03-25 | 2012-10-04 | Bayer Intellectual Property Gmbh | Use of n-(1,2,5-oxadiazol-3-yl)benzamides for controlling unwanted plants in areas of transgenic crop plants being tolerant to hppd inhibitor herbicides |
KR20140033354A (ko) | 2011-03-25 | 2014-03-18 | 바이엘 인텔렉쳐 프로퍼티 게엠베하 | Hppd 억제 제초제에 대해서 내성인 유전자이식 농작물의 영역에서 원치 않는 식물을 방제하기 위한 n-(테트라졸-4-일)- 또는 n-(트리아졸-3-일)아릴카복사미드 또는 그의 염의 용도 |
WO2012136581A1 (en) | 2011-04-08 | 2012-10-11 | Bayer Cropscience Ag | Fungicide hydroximoyl-tetrazole derivatives |
BR112013027091B1 (pt) | 2011-04-22 | 2020-12-01 | Bayer Cropscience Aktiengesellschaft | combinação de composto ativo, composição para controle de fungos nocivos fitopatogênicos, método para controle de fungos nocivos fitopatogênicos, processo para produção de composições para controle de fungos nocivos fitopatogênicos e usos de uma combinação de composto ativo |
CN102776268A (zh) * | 2011-05-09 | 2012-11-14 | 广州迪澳生物科技有限公司 | 恒温基因扩增检测转基因玉米Mon89034及其衍生品种的试剂盒及方法 |
PL2720543T3 (pl) | 2011-06-14 | 2019-03-29 | Bayer Cropscience Ag | Zastosowanie związku enaminokarbonylowego w kombinacji ze środkiem kontroli biologicznej |
CN103717076B (zh) | 2011-08-10 | 2016-04-13 | 拜耳知识产权股份有限公司 | 含有特定特特拉姆酸衍生物的活性化合物组合物 |
AU2012299691B2 (en) | 2011-08-22 | 2015-01-29 | BASF Agricultural Solutions Seed US LLC | Methods and means to modify a plant genome |
CN102952862B (zh) * | 2011-08-26 | 2014-12-31 | 深圳出入境检验检疫局动植物检验检疫技术中心 | 转基因玉米品系mon89034 pcr-dhplc检测引物及检测方法 |
EP2561759A1 (en) | 2011-08-26 | 2013-02-27 | Bayer Cropscience AG | Fluoroalkyl-substituted 2-amidobenzimidazoles and their effect on plant growth |
US9090600B2 (en) | 2011-09-12 | 2015-07-28 | Bayer Intellectual Property Gmbh | Fungicidal 4-substituted-3-{phenyl[(heterocyclylmethoxy)imino]methyl}-1,2,4-oxadizol-5(4H)-one derivatives |
CN107897194A (zh) | 2011-09-16 | 2018-04-13 | 拜耳知识产权有限责任公司 | 5‑苯基‑或5‑苄基‑2‑异噁唑啉‑3‑甲酸酯用于改善植物产量的用途 |
MX362112B (es) | 2011-09-16 | 2019-01-07 | Bayer Ip Gmbh | Uso de fenilpirazolin-3-carboxilatos para mejorar el rendimiento de las plantas. |
BR112014006217B1 (pt) | 2011-09-16 | 2019-01-15 | Bayer Intellectual Property Gmbh | utilização de acilsulfonamidas para melhorar o rendimento de plantas,método para induzir respostas de regulação de crescimento em plantas úteis ou plantas de cultura e composição. |
EA028662B1 (ru) | 2011-10-04 | 2017-12-29 | Байер Интеллекчуал Проперти Гмбх | Рнк-интерференция для борьбы с грибами и оомицетами путем ингибирования гена сахаропиндегидрогеназы |
CA2854327A1 (en) | 2011-11-02 | 2013-05-10 | University Of North Texas | Mtnip regulated plants with significantly increased size and biomass |
JP2014533666A (ja) | 2011-11-21 | 2014-12-15 | バイエル・インテレクチユアル・プロパテイー・ゲー・エム・ベー・ハー | 殺菌剤n−[(トリ置換シリル)メチル]−カルボキサミド誘導体 |
BR112014013031A2 (pt) | 2011-11-30 | 2017-06-13 | Bayer Ip Gmbh | composto, composição fungicida e método para o controle dos fungos |
WO2013092519A1 (en) | 2011-12-19 | 2013-06-27 | Bayer Cropscience Ag | Use of anthranilic acid diamide derivatives for pest control in transgenic crops |
CN104470896B (zh) | 2011-12-29 | 2016-11-09 | 拜耳知识产权有限责任公司 | 杀真菌的3-[(吡啶-2-基甲氧基亚氨基)(苯基)甲基]-2-取代的-1,2,4-噁二唑-5(2h)-酮衍生物 |
TWI558701B (zh) | 2011-12-29 | 2016-11-21 | 拜耳知識產權公司 | 殺真菌之3-[(1,3-噻唑-4-基甲氧基亞胺)(苯基)甲基]-2-經取代之-1,2,4-二唑-5(2h)-酮衍生物 |
EP2806740B1 (en) | 2012-01-25 | 2018-01-10 | Bayer Intellectual Property GmbH | Active compounds combination containing fluopyram bacillus and biologically control agent |
US20150011389A1 (en) | 2012-01-25 | 2015-01-08 | Bayer Intellectual Property Gmbh | Active Compound Combinations Containing Fluopyram and Biological Control Agent |
BR122019010667B1 (pt) | 2012-02-27 | 2020-12-22 | Bayer Intellectual Property Gmbh | combinação, método para controle de fungos fitopatogênicos prejudiciais e uso da referida combinação |
WO2013139949A1 (en) | 2012-03-23 | 2013-09-26 | Bayer Intellectual Property Gmbh | Compositions comprising a strigolactame compound for enhanced plant growth and yield |
WO2013153143A1 (en) | 2012-04-12 | 2013-10-17 | Bayer Cropscience Ag | N-acyl- 2 - (cyclo) alkylpyrrolidines and piperidines useful as fungicides |
BR112014025976B1 (pt) | 2012-04-20 | 2019-10-29 | Bayer Cropscience Ag | composto, processo para preparar um composto, composição fungicida, método para controlar fungos, uso de compostos e processo para produzir composições para controlar fungos |
CN104244717A (zh) | 2012-04-20 | 2014-12-24 | 拜尔农科股份公司 | N-环烷基-n-[(三取代的甲硅烷基苯基)亚甲基]-(硫代)羧酰胺衍生物 |
AR090558A1 (es) * | 2012-04-24 | 2014-11-19 | Pioneer Hi Bred Int | Evento de maiz dp-004114-3 y metodos para su deteccion |
US20130295674A1 (en) * | 2012-05-01 | 2013-11-07 | Pioneer Hi-Bred International, Inc. | Method of extracting embryos from kernels of corn |
UA126903C2 (uk) | 2012-05-08 | 2023-02-22 | Монсанто Текнолоджи Ллс | Об'єкт кукурудзи mon 87411 |
EP2662370A1 (en) | 2012-05-09 | 2013-11-13 | Bayer CropScience AG | 5-Halogenopyrazole benzofuranyl carboxamides |
JP6262208B2 (ja) | 2012-05-09 | 2018-01-17 | バイエル・クロップサイエンス・アクチェンゲゼルシャフト | ピラゾールインダニルカルボキサミド類 |
EP2662363A1 (en) | 2012-05-09 | 2013-11-13 | Bayer CropScience AG | 5-Halogenopyrazole biphenylcarboxamides |
EP2662361A1 (en) | 2012-05-09 | 2013-11-13 | Bayer CropScience AG | Pyrazol indanyl carboxamides |
EP2662364A1 (en) | 2012-05-09 | 2013-11-13 | Bayer CropScience AG | Pyrazole tetrahydronaphthyl carboxamides |
EP2662360A1 (en) | 2012-05-09 | 2013-11-13 | Bayer CropScience AG | 5-Halogenopyrazole indanyl carboxamides |
EP2662362A1 (en) | 2012-05-09 | 2013-11-13 | Bayer CropScience AG | Pyrazole indanyl carboxamides |
CN104364236B (zh) | 2012-05-09 | 2018-01-16 | 拜尔农作物科学股份公司 | 5‑卤代吡唑二氢茚基甲酰胺 |
AR091104A1 (es) | 2012-05-22 | 2015-01-14 | Bayer Cropscience Ag | Combinaciones de compuestos activos que comprenden un derivado lipo-quitooligosacarido y un compuesto nematicida, insecticida o fungicida |
WO2013178653A1 (en) | 2012-05-30 | 2013-12-05 | Bayer Cropscience Ag | Composition comprising a biological control agent and a fungicide selected from inhibitors of amino acid or protein biosynthesis, inhibitors of atp production and inhibitors of the cell wall synthesis |
AU2013269725B2 (en) | 2012-05-30 | 2016-10-13 | Bayer Cropscience Ag | Compositions comprising a biological control agent and an insecticide |
ES2694201T3 (es) | 2012-05-30 | 2018-12-19 | Bayer Cropscience Ag | Composición que comprende un agente de control biológico y trifloxiestrobina |
HUE040336T2 (hu) | 2012-05-30 | 2019-03-28 | Bayer Cropscience Ag | Biológiai hatóanyagot és fluopikolidot tartalmazó készítmény |
AR091196A1 (es) | 2012-05-30 | 2015-01-21 | Bayer Cropscience Ag | Composicion que comprende un agente de control biologico y un fungicida |
PT2854548T (pt) | 2012-05-30 | 2018-12-06 | Bayer Cropscience Ag | Composição compreendendo um agente de controlo biológico e um fungicida selecionado a partir de metalaxilo e metalaxil-m |
EP2854535A1 (en) | 2012-05-30 | 2015-04-08 | Bayer Cropscience AG | Compositions comprising a biological control agent and an insecticide |
EP2854550B1 (en) | 2012-05-30 | 2018-07-04 | Bayer Cropscience AG | Composition comprising a biological control agent and a fungicide |
WO2014004458A2 (en) * | 2012-06-25 | 2014-01-03 | Dow Agrosciences Llc | Insect resistant and herbicide tolerant soybean event pdab9582.816.15.1 |
CN104602520A (zh) | 2012-07-31 | 2015-05-06 | 拜尔农作物科学股份公司 | 包括杀虫萜烯混合物和杀虫剂的组合物 |
MY196630A (en) | 2012-09-14 | 2023-04-23 | BASF Agricultural Solutions Seed US LLC | Hppd Variants and Methods of use |
EP2719280A1 (en) | 2012-10-11 | 2014-04-16 | Bayer CropScience AG | Use of N-phenylethylpyrazole carboxamide derivatives or salts thereof for resistance management of phytopathogenic fungi |
US20150250176A1 (en) | 2012-10-19 | 2015-09-10 | Bayer Cropscience Ag | Method for enhancing tolerance to abiotic stress in plants using carboxamide or thiocarboxamide derivatives |
CA2888556C (en) | 2012-10-19 | 2020-07-07 | Bayer Cropscience Ag | Method of plant growth promotion using carboxamide derivatives |
CA2888562C (en) | 2012-10-19 | 2020-10-27 | Bayer Cropscience Ag | Method for treating plants against fungi resistant to fungicides using carboxamide or thiocarboxamide derivatives |
EA026839B1 (ru) | 2012-10-19 | 2017-05-31 | Байер Кропсайенс Аг | Комбинации активных соединений, содержащие карбоксамидные соединения |
EP2735231A1 (en) | 2012-11-23 | 2014-05-28 | Bayer CropScience AG | Active compound combinations |
BR112015012519A2 (pt) | 2012-11-30 | 2017-07-11 | Bayer Cropscience Ag | misturas ternárias fungicidas e pesticidas |
US9615578B2 (en) | 2012-11-30 | 2017-04-11 | Bayer Cropscience Ag | Binary fungicidal mixtures |
EA031510B1 (ru) | 2012-11-30 | 2019-01-31 | Байер Кропсайенс Акциенгезельшафт | Двойная фунгицидная смесь |
EA030020B1 (ru) | 2012-11-30 | 2018-06-29 | Байер Кропсайенс Акциенгезельшафт | Двойные фунгицидные смеси |
PL2925134T3 (pl) | 2012-11-30 | 2020-06-29 | Bayer Cropscience Ag | Trójskładnikowe mieszaniny grzybobójcze |
WO2014086753A2 (en) | 2012-12-03 | 2014-06-12 | Bayer Cropscience Ag | Composition comprising biological control agents |
ES2667555T3 (es) | 2012-12-03 | 2018-05-11 | Bayer Cropscience Ag | Composición que comprende un agente de control biológico y un insecticida |
MX2015006631A (es) | 2012-12-03 | 2015-08-05 | Bayer Cropscience Ag | Composicion que comprende un agente de control biologico y un insecticida. |
MA38142A1 (fr) | 2012-12-03 | 2016-02-29 | Bayer Cropscience Ag | Composition comprenant un agent de lutte biologique et un fongicide |
BR112015012781A2 (pt) | 2012-12-03 | 2018-06-26 | Bayer Cropscience Ag | composição compreendendo agentes de controle biológico |
EP2925144A2 (en) | 2012-12-03 | 2015-10-07 | Bayer CropScience AG | Composition comprising a biological control agent and an insecticide |
WO2014086747A2 (en) | 2012-12-03 | 2014-06-12 | Bayer Cropscience Ag | Composition comprising a biological control agent and a fungicide |
US9763454B2 (en) | 2012-12-03 | 2017-09-19 | Bayer Cropscience Ag | Composition comprising a biological control agent and a fungicide |
EP2929055A4 (en) * | 2012-12-10 | 2016-07-27 | Agrigenetics Inc | RECOVERY OF GENOMIC DNA FROM REMAINING EXTRACTED SEED SAMPLES |
AR093909A1 (es) | 2012-12-12 | 2015-06-24 | Bayer Cropscience Ag | Uso de ingredientes activos para controlar nematodos en cultivos resistentes a nematodos |
AR093996A1 (es) | 2012-12-18 | 2015-07-01 | Bayer Cropscience Ag | Combinaciones bactericidas y fungicidas binarias |
US9428459B2 (en) | 2012-12-19 | 2016-08-30 | Bayer Cropscience Ag | Difluoromethyl-nicotinic- tetrahydronaphtyl carboxamides |
CA2899490A1 (en) | 2013-02-11 | 2014-08-14 | Bayer Cropscience Lp | Compositions comprising gougerotin and a fungicide |
JP2016506973A (ja) | 2013-02-11 | 2016-03-07 | バイエル クロップサイエンス エルピーBayer Cropscience Lp | グーゲロチンおよび殺虫剤を含む組成物 |
EP2953467A1 (en) | 2013-02-11 | 2015-12-16 | Bayer Cropscience LP | Compositions comprising gougerotin and a biological control agent |
WO2014138339A2 (en) | 2013-03-07 | 2014-09-12 | Athenix Corp. | Toxin genes and methods for their use |
JP2016519687A (ja) | 2013-04-19 | 2016-07-07 | バイエル・クロップサイエンス・アクチェンゲゼルシャフト | バイナリー殺虫または農薬混合物 |
CN105555135B (zh) | 2013-04-19 | 2018-06-15 | 拜耳作物科学股份公司 | 涉及邻苯二甲酰胺衍生物应用的用于改善对转基因植物生产潜能的利用的方法 |
TW201507722A (zh) | 2013-04-30 | 2015-03-01 | Bayer Cropscience Ag | 做為殺線蟲劑及殺體內寄生蟲劑的n-(2-鹵素-2-苯乙基)-羧醯胺類 |
WO2014177514A1 (en) | 2013-04-30 | 2014-11-06 | Bayer Cropscience Ag | Nematicidal n-substituted phenethylcarboxamides |
US9365863B2 (en) | 2013-05-08 | 2016-06-14 | Monsanto Technology Llc | Compositions and methods for deploying a transgenic refuge seed blend |
UA124407C2 (uk) * | 2013-06-14 | 2021-09-15 | Монсанто Текнолоджі Елелсі | Рекомбінантна молекула днк, яка надає рослині стійкості до лускокрилої комахи-шкідника, і спосіб її виявлення та використання |
US9770022B2 (en) | 2013-06-26 | 2017-09-26 | Bayer Cropscience Ag | N-cycloalkyl-N-[(bicyclylphenyl)methylene]-(thio)carboxamide derivatives |
AR097995A1 (es) | 2013-10-14 | 2016-04-27 | Syngenta Participations Ag | Método para sembrar filas de cultivos |
CN103636653B (zh) * | 2013-11-21 | 2015-07-15 | 北京大北农科技集团股份有限公司 | 控制害虫的方法 |
CN105873907B (zh) | 2013-12-05 | 2019-03-12 | 拜耳作物科学股份公司 | N-环烷基-n-{[2-(1-取代的环烷基)苯基]亚甲基}-(硫代)甲酰胺衍生物 |
ES2705577T3 (es) | 2013-12-05 | 2019-03-26 | Bayer Cropscience Ag | Derivados de N-ciclopropil-N-{[2-(1-ciclopropil sustituido)fenil]metileno}-(tio)carboxamida |
EP2885970A1 (en) | 2013-12-21 | 2015-06-24 | Bayer CropScience AG | Fungicide compositions comprising compound I, at least one succinate dehydrogenase (SDH) inhibitor and at least one triazole fungicide |
ES2806473T3 (es) * | 2014-02-07 | 2021-02-17 | Pioneer Hi Bred Int | Proteínas insecticidas y métodos para su uso |
BR112016020889B1 (pt) | 2014-03-11 | 2022-10-04 | BASF Agricultural Solutions Seed US LLC | Molécula de ácido nucleico recombinante, célula hospedeira bacteriana, proteína hppd recombinante, uso do ácido nucleico recombinante e produto de base |
WO2015160618A1 (en) | 2014-04-16 | 2015-10-22 | Bayer Cropscience Lp | Compositions comprising ningnanmycin and a biological control agent |
WO2015160619A1 (en) | 2014-04-16 | 2015-10-22 | Bayer Cropscience Lp | Compositions comprising ningnanmycin and a fungicide |
WO2015160620A1 (en) | 2014-04-16 | 2015-10-22 | Bayer Cropscience Lp | Compositions comprising ningnanmycin and an insecticide |
CN105446890B (zh) * | 2014-09-18 | 2020-08-25 | 三星电子株式会社 | 智能数据部署 |
ES2914178T3 (es) | 2014-10-15 | 2022-06-07 | Monsanto Technology Llc | Genes de tolerancia a herbicidas y métodos para usar los mismos |
US9510533B2 (en) | 2014-11-13 | 2016-12-06 | Seminis Vegetable Seeds, Inc. | Sweet corn hybrid SV1446SD and parents thereof |
US9374962B2 (en) | 2014-11-13 | 2016-06-28 | Seminis Vegetable Seeds, Inc. | Sweet corn hybrid SV9014SB and parents thereof |
PE20170955A1 (es) | 2014-11-20 | 2017-07-13 | Monsanto Technology Llc | Nuevas proteinas inhibidoras de insectos |
US11130964B2 (en) | 2014-11-20 | 2021-09-28 | Monsanto Technology Llc | Insect inhibitory proteins |
CN104522033B (zh) * | 2014-12-22 | 2016-09-14 | 北京大北农科技集团股份有限公司 | 杀虫蛋白的用途 |
US10214510B2 (en) | 2015-04-13 | 2019-02-26 | Bayer Cropscience Aktiengesellschaft | N-cycloalkyl-N-(biheterocyclylethylene)-(thio)carboxamide derivatives |
CN104830847B (zh) * | 2015-04-30 | 2019-01-11 | 北京大北农科技集团股份有限公司 | 用于检测玉米植物dbn9936的核酸序列及其检测方法 |
CN104878094B (zh) * | 2015-04-30 | 2019-01-15 | 北京大北农科技集团股份有限公司 | 用于检测除草剂耐受性玉米植物dbn9888的核酸序列及其检测方法 |
CN104878091B (zh) * | 2015-04-30 | 2019-05-17 | 北京大北农科技集团股份有限公司 | 用于检测玉米植物dbn9978的核酸序列及其检测方法 |
CN104878096B (zh) * | 2015-04-30 | 2019-01-15 | 北京大北农科技集团股份有限公司 | 用于检测除草剂耐受性玉米植物dbn9868的核酸序列及其检测方法 |
CN104846084B (zh) * | 2015-04-30 | 2019-02-01 | 北京大北农科技集团股份有限公司 | 用于检测玉米植物dbn9927的核酸序列及其检测方法 |
CN104878095B (zh) * | 2015-04-30 | 2018-10-26 | 北京大北农科技集团股份有限公司 | 用于检测除草剂耐受性玉米植物dbn9858的核酸序列及其检测方法 |
CN104920425B (zh) * | 2015-05-20 | 2018-06-19 | 北京大北农科技集团股份有限公司 | 杀虫蛋白的用途 |
EP3097782A1 (en) | 2015-05-29 | 2016-11-30 | Bayer CropScience Aktiengesellschaft | Methods for controlling phytopathogenic nematodes by combination of fluopyram and biological control agents |
AU2016319093B2 (en) | 2015-09-11 | 2022-11-03 | BASF Agricultural Solutions Seed US LLC | HPPD variants and methods of use |
CN105994169B (zh) * | 2016-05-31 | 2019-04-23 | 中国农业科学院植物保护研究所 | 一种利用嫩玉米穗饲养大螟的方法 |
US9877451B2 (en) | 2016-06-09 | 2018-01-30 | Seminis Vegetable Seeds, Inc. | Sweet corn hybrid SV7538SK and parents thereof |
US9888649B2 (en) | 2016-06-09 | 2018-02-13 | Seminis Vegetable Seeds, Inc. | Sweet corn hybrid SVSK0391 and parents thereof |
US9980452B2 (en) | 2016-06-09 | 2018-05-29 | Seminis Vegetable Seeds, Inc. | Sweet corn line SHY-6RLAN014 |
US9980451B2 (en) | 2016-06-09 | 2018-05-29 | Seminis Vegetable Seeds, Inc. | Sweet corn line SHY-6RLAL196 |
US10028463B2 (en) | 2016-07-11 | 2018-07-24 | Seminis Vegetable Seeds, Inc. | Sweet corn line SYW-6SSLM804 |
WO2018019676A1 (en) | 2016-07-29 | 2018-02-01 | Bayer Cropscience Aktiengesellschaft | Active compound combinations and methods to protect the propagation material of plants |
MX2019005835A (es) | 2016-11-23 | 2019-10-30 | BASF Agricultural Solutions Seed US LLC | Genes de toxinas axmi669 y axmi991 y metodos para su uso. |
US20190322631A1 (en) | 2016-12-19 | 2019-10-24 | Basf Se | Substituted oxadiazoles for combating phytopathogenic fungi |
JP2020504609A (ja) | 2016-12-22 | 2020-02-13 | ビーエーエスエフ アグリカルチュラル ソリューションズ シード ユーエス エルエルシー | 有害線虫の防除のためのcry14の使用 |
AR110756A1 (es) | 2017-01-18 | 2019-05-02 | Bayer Cropscience Lp | Uso de bp005 para el control de patógenos de planta |
CN110431234B (zh) | 2017-01-18 | 2024-04-16 | 巴斯夫农业种子解决方案美国有限责任公司 | Bp005毒素基因及其使用方法 |
WO2018153730A1 (en) | 2017-02-21 | 2018-08-30 | Basf Se | Substituted oxadiazoles for combating phytopathogenic fungi |
US11708565B2 (en) | 2017-03-07 | 2023-07-25 | BASF Agricultural Solutions Seesi US LLC | HPPD variants and methods of use |
AU2018247768A1 (en) | 2017-04-07 | 2019-10-03 | Basf Se | Substituted oxadiazoles for combating phytopathogenic fungi |
WO2018188962A1 (en) | 2017-04-11 | 2018-10-18 | Basf Se | Substituted oxadiazoles for combating phytopathogenic fungi |
EP3612029A1 (en) | 2017-04-21 | 2020-02-26 | Bayer CropScience LP | Method of improving crop safety |
WO2018202491A1 (en) | 2017-05-04 | 2018-11-08 | Basf Se | Substituted trifluoromethyloxadiazoles for combating phytopathogenic fungi |
CN110621669A (zh) | 2017-05-04 | 2019-12-27 | 巴斯夫欧洲公司 | 防除植物病原性真菌的取代5-卤代烷基-5-羟基异噁唑类 |
WO2018219797A1 (en) | 2017-06-02 | 2018-12-06 | Basf Se | Substituted oxadiazoles for combating phytopathogenic fungi |
US10212900B2 (en) | 2017-06-08 | 2019-02-26 | Seminis Vegetable Seeds, Inc. | Sweet corn hybrid SVSC0111 and parents thereof |
US10244701B2 (en) | 2017-06-08 | 2019-04-02 | Seminis Vegetable Seeds, Inc. | Sweet corn hybrid SVSD7749 and parents thereof |
US10188058B2 (en) | 2017-06-08 | 2019-01-29 | Seminis Vegetable Seeds, Inc. | Sweet corn hybrid SVSK6143 and parents thereof |
US10194611B2 (en) | 2017-06-08 | 2019-02-05 | Seminis Vegetable Seeds, Inc. | Sweet corn hybrid SVSB3847 and parents thereof |
EP3642187A1 (en) | 2017-06-19 | 2020-04-29 | Basf Se | 2-[[5-(trifluoromethyl)-1,2,4-oxadiazol-3-yl]aryloxy](thio)acetamides for combating phytopathogenic fungi |
WO2019025250A1 (en) | 2017-08-04 | 2019-02-07 | Basf Se | SUBSTITUTED TRIFLUOROMETHYLOXADIAZOLES FOR COMBATING PHYTOPATHOGENIC FUNGI |
WO2019038042A1 (en) | 2017-08-21 | 2019-02-28 | Basf Se | SUBSTITUTED TRIFLUOROMETHYLOXADIAZOLES FOR THE CONTROL OF PHYTOPATHOGENIC FUNGI |
US11076596B2 (en) | 2017-09-18 | 2021-08-03 | Basf Se | Substituted trifluoromethyloxadiazoles for combating phytopathogenic fungi |
WO2019068811A1 (en) | 2017-10-06 | 2019-04-11 | Bayer Aktiengesellschaft | COMPOSITIONS COMPRISING FLUOPYRAM AND TIOXAZAFENE |
BR112020008096A2 (pt) | 2017-10-24 | 2020-11-03 | Basf Se | método para conferir tolerância a um herbicida e planta de soja transgênica |
WO2019083810A1 (en) | 2017-10-24 | 2019-05-02 | Basf Se | IMPROVING HERBICIDE TOLERANCE FOR 4-HYDROXYPHENYLPYRUVATE DIOXYGENASE (HPPD) INHIBITORS BY NEGATIVE REGULATION OF HPPD EXPRESSION IN SOYBEANS |
US11147275B2 (en) | 2017-11-23 | 2021-10-19 | Basf Se | Substituted trifluoromethyloxadiazoles for combating phytopathogenic fungi |
US11834466B2 (en) | 2017-11-30 | 2023-12-05 | 5Metis, Inc. | Benzoxaborole compounds and formulations thereof |
WO2019121143A1 (en) | 2017-12-20 | 2019-06-27 | Basf Se | Substituted cyclopropyl derivatives |
WO2019137995A1 (en) | 2018-01-11 | 2019-07-18 | Basf Se | Novel pyridazine compounds for controlling invertebrate pests |
BR112020013680A2 (pt) | 2018-01-29 | 2020-12-01 | BASF Agro B.V. | formulações agroquímicas, uso de formulações e método para controlar insetos |
JP2021512887A (ja) | 2018-02-07 | 2021-05-20 | ビーエイエスエフ・ソシエタス・エウロパエアBasf Se | 新規ピリジンカルボキサミド |
WO2019154665A1 (en) | 2018-02-07 | 2019-08-15 | Basf Se | New pyridine carboxamides |
WO2019166257A1 (en) | 2018-03-01 | 2019-09-06 | BASF Agro B.V. | Fungicidal compositions of mefentrifluconazole |
WO2019219464A1 (en) | 2018-05-15 | 2019-11-21 | Basf Se | Substituted trifluoromethyloxadiazoles for combating phytopathogenic fungi |
WO2019224092A1 (en) | 2018-05-22 | 2019-11-28 | Basf Se | Pesticidally active c15-derivatives of ginkgolides |
US20210323950A1 (en) | 2018-06-04 | 2021-10-21 | Bayer Aktiengesellschaft | Herbicidally active bicyclic benzoylpyrazoles |
CR20210046A (es) | 2018-07-30 | 2021-06-10 | Monsanto Technology Llc | Evento transgénico de maíz mon 95379 y métodos para su detección y usos |
CA3148168A1 (en) | 2018-08-18 | 2020-02-27 | Chun Yu Liu | Solid forms of substituted benzoxaborole and compositions thereof |
EP3613736A1 (en) | 2018-08-22 | 2020-02-26 | Basf Se | Substituted glutarimide derivatives |
EP3628158A1 (en) | 2018-09-28 | 2020-04-01 | Basf Se | Pesticidal mixture comprising a mesoionic compound and a biopesticide |
UA127747C2 (uk) | 2018-10-23 | 2023-12-20 | Басф Се | Трициклічні пестицидні сполуки |
EP3643705A1 (en) | 2018-10-24 | 2020-04-29 | Basf Se | Pesticidal compounds |
EP3670501A1 (en) | 2018-12-17 | 2020-06-24 | Basf Se | Substituted [1,2,4]triazole compounds as fungicides |
FI3908584T3 (fi) | 2019-01-11 | 2023-06-19 | Basf Se | 1-(1,2-dimetyylipropyyli)-N-etyyli-5-metyyli-N-pyridatsin-4-yylipyratsoli-4-karboksamidin kiteisiä muotoja |
EP3696177A1 (en) | 2019-02-12 | 2020-08-19 | Basf Se | Heterocyclic compounds for the control of invertebrate pests |
CN109735605A (zh) * | 2019-02-18 | 2019-05-10 | 上海市计量测试技术研究院(中国上海测试中心、华东国家计量测试中心、上海市计量器具强制检定中心) | 转基因玉米mon89034品系定量检测试剂盒及数字pcr检测方法 |
CA3139524A1 (en) | 2019-05-10 | 2020-11-19 | Bayer Cropscience Lp | Active compound combinations |
EP3769623A1 (en) | 2019-07-22 | 2021-01-27 | Basf Se | Mesoionic imidazolium compounds and derivatives for combating animal pests |
BR112021019416A2 (pt) | 2019-05-29 | 2021-12-07 | Basf Se | Compostos, composição, métodos de proteção de safras e de combate, controle, prevenção ou proteção contra infestações, método não terapêutico de tratamento de animais infestados, semente e uso |
WO2020244970A1 (en) | 2019-06-06 | 2020-12-10 | Basf Se | New carbocyclic pyridine carboxamides |
AU2020286573A1 (en) | 2019-06-06 | 2021-12-23 | Basf Se | Fungicidal n-(pyrid-3-yl)carboxamides |
WO2020244969A1 (en) | 2019-06-06 | 2020-12-10 | Basf Se | Pyridine derivatives and their use as fungicides |
CN110317896B (zh) * | 2019-06-19 | 2023-05-26 | 许昌学院 | 用于检测玉米源成分的lamp引物组及其用途 |
EP3766879A1 (en) | 2019-07-19 | 2021-01-20 | Basf Se | Pesticidal pyrazole derivatives |
TW202118392A (zh) | 2019-07-22 | 2021-05-16 | 德商拜耳廠股份有限公司 | 作為殺蟲劑之5-胺基取代的吡唑及三唑 |
AU2020318590A1 (en) | 2019-07-23 | 2022-02-17 | Bayer Aktiengesellschaft | Novel heteroaryl-triazole compounds as pesticides |
CA3148216A1 (en) | 2019-07-23 | 2021-01-28 | Bayer Aktiengesellschaft | Novel heteroaryl-triazole compounds as pesticides |
EP4007494A1 (en) | 2019-08-01 | 2022-06-08 | Bayer CropScience LP | Method of improving cold stress tolerance and crop safety |
EP3701796A1 (en) | 2019-08-08 | 2020-09-02 | Bayer AG | Active compound combinations |
EP4034656A1 (en) | 2019-09-26 | 2022-08-03 | Bayer Aktiengesellschaft | Rnai-mediated pest control |
US20220361492A1 (en) | 2019-10-02 | 2022-11-17 | Bayer Aktiengesellschaft | Active compound combinations comprising fatty acids |
WO2021063736A1 (en) | 2019-10-02 | 2021-04-08 | Basf Se | Bicyclic pyridine derivatives |
WO2021063735A1 (en) | 2019-10-02 | 2021-04-08 | Basf Se | New bicyclic pyridine derivatives |
BR112022006791A2 (pt) | 2019-10-09 | 2022-06-28 | Bayer Ag | Novos compostos heteroaril-triazol como pesticidas |
BR112022006774A2 (pt) | 2019-10-09 | 2022-06-28 | Bayer Ag | Compostos de heteroaril-triazol inovadores como pesticidas |
WO2021089673A1 (de) | 2019-11-07 | 2021-05-14 | Bayer Aktiengesellschaft | Substituierte sulfonylamide zur bekämpfung tierischer schädlinge |
WO2021097162A1 (en) | 2019-11-13 | 2021-05-20 | Bayer Cropscience Lp | Beneficial combinations with paenibacillus |
WO2021099271A1 (en) | 2019-11-18 | 2021-05-27 | Bayer Aktiengesellschaft | Active compound combinations comprising fatty acids |
TW202134226A (zh) | 2019-11-18 | 2021-09-16 | 德商拜耳廠股份有限公司 | 作為殺蟲劑之新穎雜芳基-三唑化合物 |
TW202136248A (zh) | 2019-11-25 | 2021-10-01 | 德商拜耳廠股份有限公司 | 作為殺蟲劑之新穎雜芳基-三唑化合物 |
CN115335392A (zh) | 2020-01-31 | 2022-11-11 | 成对植物服务股份有限公司 | 植物避荫反应的抑制 |
BR112022016400A2 (pt) | 2020-02-18 | 2022-10-25 | Bayer Ag | Compostos inovadores de heteroaril-triazol como pesticidas |
EP3708565A1 (en) | 2020-03-04 | 2020-09-16 | Bayer AG | Pyrimidinyloxyphenylamidines and the use thereof as fungicides |
MX2022012878A (es) | 2020-04-16 | 2022-12-08 | Pairwise Plants Services Inc | Metodos para controlar el tama?o del meristema para la mejora de cultivos. |
WO2021209490A1 (en) | 2020-04-16 | 2021-10-21 | Bayer Aktiengesellschaft | Cyclaminephenylaminoquinolines as fungicides |
JP2023522350A (ja) | 2020-04-21 | 2023-05-30 | バイエル・アクチエンゲゼルシヤフト | 有害生物防除剤としての2-(ヘタ)アリール-置換縮合ヘテロ環誘導体 |
CA3179211A1 (en) * | 2020-04-24 | 2021-10-28 | Monsanto Technology Llc | Transgenic corn event mon95275 and methods for detection and uses thereof |
EP3903582A1 (en) | 2020-04-28 | 2021-11-03 | Basf Se | Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors ii |
EP3903581A1 (en) | 2020-04-28 | 2021-11-03 | Basf Se | Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors i |
EP4143167B1 (en) | 2020-04-28 | 2024-05-15 | Basf Se | Pesticidal compounds |
EP3903583A1 (en) | 2020-04-28 | 2021-11-03 | Basf Se | Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors iii |
EP3903584A1 (en) | 2020-04-28 | 2021-11-03 | Basf Se | Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors iv |
TW202208347A (zh) | 2020-05-06 | 2022-03-01 | 德商拜耳廠股份有限公司 | 作為殺蟲劑之新穎雜芳基三唑化合物 |
EP4146628A1 (en) | 2020-05-06 | 2023-03-15 | Bayer Aktiengesellschaft | Pyridine (thio)amides as fungicidal compounds |
WO2021228734A1 (en) | 2020-05-12 | 2021-11-18 | Bayer Aktiengesellschaft | Triazine and pyrimidine (thio)amides as fungicidal compounds |
EP3909950A1 (en) | 2020-05-13 | 2021-11-17 | Basf Se | Heterocyclic compounds for the control of invertebrate pests |
EP4153566A1 (en) | 2020-05-19 | 2023-03-29 | Bayer CropScience Aktiengesellschaft | Azabicyclic(thio)amides as fungicidal compounds |
CA3185017A1 (en) | 2020-06-02 | 2021-12-09 | Pairwise Plants Services, Inc. | Methods for controlling meristem size for crop improvement |
WO2021245087A1 (en) | 2020-06-04 | 2021-12-09 | Bayer Aktiengesellschaft | Heterocyclyl pyrimidines and triazines as novel fungicides |
CA3186659A1 (en) | 2020-06-10 | 2021-12-16 | Bayer Aktiengesellschaft | Azabicyclyl-substituted heterocycles as fungicides |
WO2021249800A1 (en) | 2020-06-10 | 2021-12-16 | Basf Se | Substituted [1,2,4]triazole compounds as fungicides |
EP3945089A1 (en) | 2020-07-31 | 2022-02-02 | Basf Se | Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors v |
BR112022025598A2 (pt) | 2020-06-17 | 2023-01-03 | Pairwise Plants Services Inc | Métodos para controlar o tamanho do meristema para melhoria da safra |
BR112022025941A2 (pt) | 2020-06-18 | 2023-01-10 | Bayer Ag | Derivados de 3-(piridazin-4-il)-5,6-di-hidro-4h-1,2,4-oxadiazina como fungicidas para proteção de cultura |
BR112022025344A2 (pt) | 2020-06-18 | 2023-01-03 | Bayer Ag | Composição para uso na agricultura |
BR112022025692A2 (pt) | 2020-06-19 | 2023-02-28 | Bayer Ag | 1,3,4-oxadiazóis e seus derivados como fungicidas |
UY39275A (es) | 2020-06-19 | 2022-01-31 | Bayer Ag | 1,3,4-oxadiazol pirimidinas como fungicidas, procesos e intermediarios para su preparación, métodos de uso y usos de los mismos |
WO2021255089A1 (en) | 2020-06-19 | 2021-12-23 | Bayer Aktiengesellschaft | 1,3,4-oxadiazole pyrimidines and 1,3,4-oxadiazole pyridines as fungicides |
UY39276A (es) | 2020-06-19 | 2022-01-31 | Bayer Ag | Uso de compuestos de 1,3,4–oxadiazol–2–ilpirimidina para controlar microorganismos fitopatógenos, métodos de uso y composiciones. |
EP3929189A1 (en) | 2020-06-25 | 2021-12-29 | Bayer Animal Health GmbH | Novel heteroaryl-substituted pyrazine derivatives as pesticides |
BR112022026904A2 (pt) | 2020-07-02 | 2023-01-24 | Bayer Ag | Derivados de heterocicleno como agentes de controle de pragas |
EP3939961A1 (en) | 2020-07-16 | 2022-01-19 | Basf Se | Strobilurin type compounds and their use for combating phytopathogenic fungi |
WO2022017836A1 (en) | 2020-07-20 | 2022-01-27 | BASF Agro B.V. | Fungicidal compositions comprising (r)-2-[4-(4-chlorophenoxy)-2-(trifluoromethyl)phenyl]-1- (1,2,4-triazol-1-yl)propan-2-ol |
US20240011042A1 (en) | 2020-07-31 | 2024-01-11 | Inari Agriculture Technology, Inc. | Excisable plant transgenic loci with signature protospacer adjacent motifs or signature guide rna recognition sites |
BR112023001804A2 (pt) * | 2020-07-31 | 2023-02-23 | Inari Agriculture Tech Inc | Célula de planta de milho transgênico, parte de planta de milho transgênico, planta de milho transgênico, método para obter uma população a granel de sementes endogâmicas, método de obtenção de uma semente de milho híbrido, molécula de dna, produto de planta de milho transgênico processado, amostra biológica, molécula de ácido nucleico adaptada para detecção de dna genômico, método para detectar uma célula de planta de milho que compreende o lócus transgênico inir6, método de excisão do lócus transgênico inir6 do genoma da célula de planta de milho |
EP3970494A1 (en) | 2020-09-21 | 2022-03-23 | Basf Se | Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors viii |
WO2022033991A1 (de) | 2020-08-13 | 2022-02-17 | Bayer Aktiengesellschaft | 5-amino substituierte triazole als schädlingsbekämpfungsmittel |
WO2022053453A1 (de) | 2020-09-09 | 2022-03-17 | Bayer Aktiengesellschaft | Azolcarboxamide als schädlingsbekämpfungsmittel |
CN112080501B (zh) * | 2020-09-10 | 2021-11-23 | 隆平生物技术(海南)有限公司 | 重组启动子、基因表达盒及其在植物育种中的应用 |
WO2022058327A1 (en) | 2020-09-15 | 2022-03-24 | Bayer Aktiengesellschaft | Substituted ureas and derivatives as new antifungal agents |
EP3974414A1 (de) | 2020-09-25 | 2022-03-30 | Bayer AG | 5-amino substituierte pyrazole und triazole als schädlingsbekämpfungsmittel |
CN116209355A (zh) | 2020-10-27 | 2023-06-02 | 巴斯夫农业公司 | 包含氯氟醚菌唑的组合物 |
WO2022090069A1 (en) | 2020-11-02 | 2022-05-05 | Basf Se | Compositions comprising mefenpyr-diethyl |
WO2022090071A1 (en) | 2020-11-02 | 2022-05-05 | Basf Se | Use of mefenpyr-diethyl for controlling phytopathogenic fungi |
CN112280743B (zh) * | 2020-11-04 | 2023-06-02 | 北京粮元生物科技有限公司 | 玉米事件2a-7及其鉴定方法 |
WO2022106304A1 (en) | 2020-11-23 | 2022-05-27 | BASF Agro B.V. | Compositions comprising mefentrifluconazole |
WO2022128524A1 (en) | 2020-12-14 | 2022-06-23 | Basf Se | Sulfoximine pesticides |
EP3915971A1 (en) | 2020-12-16 | 2021-12-01 | Bayer Aktiengesellschaft | Phenyl-s(o)n-phenylamidines and the use thereof as fungicides |
WO2022129188A1 (en) | 2020-12-18 | 2022-06-23 | Bayer Aktiengesellschaft | 1,2,4-oxadiazol-3-yl pyrimidines as fungicides |
EP4262394A1 (en) | 2020-12-18 | 2023-10-25 | Bayer Aktiengesellschaft | Use of dhodh inhibitor for controlling resistant phytopathogenic fungi in crops |
WO2022129190A1 (en) | 2020-12-18 | 2022-06-23 | Bayer Aktiengesellschaft | (hetero)aryl substituted 1,2,4-oxadiazoles as fungicides |
WO2022129196A1 (en) | 2020-12-18 | 2022-06-23 | Bayer Aktiengesellschaft | Heterobicycle substituted 1,2,4-oxadiazoles as fungicides |
CN112831584B (zh) * | 2021-01-27 | 2023-06-06 | 隆平生物技术(海南)有限公司 | 转基因玉米事件lp007-2及其检测方法 |
EP4036083A1 (de) | 2021-02-02 | 2022-08-03 | Bayer Aktiengesellschaft | 5-oxy substituierte hetereozyklen, als schädlingsbekämpfungsmittel |
EP4043444A1 (en) | 2021-02-11 | 2022-08-17 | Basf Se | Substituted isoxazoline derivatives |
US20220259612A1 (en) | 2021-02-11 | 2022-08-18 | Pairwise Plants Services, Inc. | Methods and compositions for modifying cytokinin oxidase levels in plants |
WO2022182834A1 (en) | 2021-02-25 | 2022-09-01 | Pairwise Plants Services, Inc. | Methods and compositions for modifying root architecture in plants |
CN112813189B (zh) * | 2021-03-12 | 2023-01-31 | 浙江经贸职业技术学院 | 利用四重实时荧光pcr快速鉴定转基因玉米品系的方法 |
WO2022207494A1 (en) | 2021-03-30 | 2022-10-06 | Bayer Aktiengesellschaft | 3-(hetero)aryl-5-chlorodifluoromethyl-1,2,4-oxadiazole as fungicide |
BR112023019788A2 (pt) | 2021-03-30 | 2023-11-07 | Bayer Ag | 3-(hetero)aril-5-clorodifluorometil-1,2,4-oxadiazol como fungicida |
EP4333616A1 (en) | 2021-05-03 | 2024-03-13 | Basf Se | Additives for enhancing the pesticidal effectiveness of pesticidal microorganisms |
BR112023022763A2 (pt) | 2021-05-06 | 2024-01-02 | Bayer Ag | Imidazóis anulados substituídos por alquilamida e uso dos mesmos como inseticidas |
JP2024517305A (ja) | 2021-05-12 | 2024-04-19 | バイエル・アクチエンゲゼルシヤフト | 有害生物防除剤としての2-(ヘテロ)アリール置換縮合複素環誘導体 |
EP4091451A1 (en) | 2021-05-17 | 2022-11-23 | BASF Agro B.V. | Compositions comprising mefentrifluconazole |
IL308529A (en) | 2021-05-18 | 2024-01-01 | Basf Se | New converted pyridines as fungicides |
BR112023023989A2 (pt) | 2021-05-18 | 2024-01-30 | Basf Se | Compostos, composição, método para combater fungos fitopatogênicos e semente |
JP2024519813A (ja) | 2021-05-18 | 2024-05-21 | ビーエーエスエフ ソシエタス・ヨーロピア | 殺菌剤としての新規な置換ピリジン |
AR126172A1 (es) | 2021-06-17 | 2023-09-27 | Pairwise Plants Services Inc | Modificación de factores de transcripción de la familia de factores reguladores del crecimiento en soja |
UY39827A (es) | 2021-06-24 | 2023-01-31 | Pairwise Plants Services Inc | Modificación de genes de ubiquitina ligasa e3 hect para mejorar los rasgos de rendimiento |
WO2023278651A1 (en) | 2021-07-01 | 2023-01-05 | Pairwise Plants Services, Inc. | Methods and compositions for enhancing root system development |
EP4119547A1 (en) | 2021-07-12 | 2023-01-18 | Basf Se | Triazole compounds for the control of invertebrate pests |
WO2023011958A1 (en) | 2021-08-02 | 2023-02-09 | Basf Se | (3-pirydyl)-quinazoline |
CN117794908A (zh) | 2021-08-02 | 2024-03-29 | 巴斯夫欧洲公司 | (3-喹啉基)-喹唑啉 |
US20230078990A1 (en) | 2021-08-12 | 2023-03-16 | Pairwise Plants Services, Inc. | Modification of brassinosteroid receptor genes to improve yield traits |
CN118102874A (zh) | 2021-08-13 | 2024-05-28 | 拜耳公司 | 活性化合物组合以及包含它们的杀真菌剂组合物 |
US20230063927A1 (en) | 2021-08-17 | 2023-03-02 | Pairwise Plants Services, Inc. | Methods and compositions for modifying cytokinin receptor histidine kinase genes in plants |
EP4140986A1 (en) | 2021-08-23 | 2023-03-01 | Basf Se | Pyrazine compounds for the control of invertebrate pests |
AU2022335669A1 (en) | 2021-08-25 | 2024-02-01 | Bayer Aktiengesellschaft | Novel pyrazinyl-triazole compounds as pesticides |
EP4140995A1 (en) | 2021-08-27 | 2023-03-01 | Basf Se | Pyrazine compounds for the control of invertebrate pests |
US20230074699A1 (en) | 2021-08-30 | 2023-03-09 | Pairwise Plants Services, Inc. | Modification of ubiquitin binding peptidase genes in plants for yield trait improvement |
EP4144739A1 (de) | 2021-09-02 | 2023-03-08 | Bayer Aktiengesellschaft | Anellierte pyrazole als schädlingsbekämpfungsmittel |
AR126938A1 (es) | 2021-09-02 | 2023-11-29 | Pairwise Plants Services Inc | Métodos y composiciones para mejorar la arquitectura de las plantas y los rasgos de rendimiento |
EP4151631A1 (en) | 2021-09-20 | 2023-03-22 | Basf Se | Heterocyclic compounds for the control of invertebrate pests |
US20230087522A1 (en) | 2021-09-21 | 2023-03-23 | Pairwise Plants Services, Inc. | Methods and compositions for reducing pod shatter in canola |
AR127236A1 (es) | 2021-10-04 | 2024-01-03 | Pairwise Plants Services Inc | Métodos para mejorar la fertilidad de la flor y el rendimiento de semillas |
US20230116819A1 (en) | 2021-10-07 | 2023-04-13 | Pairwise Plants Services, Inc. | Methods for improving floret fertility and seed yield |
WO2023072671A1 (en) | 2021-10-28 | 2023-05-04 | Basf Se | Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors ix |
WO2023072670A1 (en) | 2021-10-28 | 2023-05-04 | Basf Se | Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors x |
WO2023078915A1 (en) | 2021-11-03 | 2023-05-11 | Bayer Aktiengesellschaft | Bis(hetero)aryl thioether (thio)amides as fungicidal compounds |
WO2023099445A1 (en) | 2021-11-30 | 2023-06-08 | Bayer Aktiengesellschaft | Bis(hetero)aryl thioether oxadiazines as fungicidal compounds |
EP4194453A1 (en) | 2021-12-08 | 2023-06-14 | Basf Se | Pyrazine compounds for the control of invertebrate pests |
AR127904A1 (es) | 2021-12-09 | 2024-03-06 | Pairwise Plants Services Inc | Métodos para mejorar la fertilidad de floretes y el rendimiento de semillas |
EP4198033A1 (en) | 2021-12-14 | 2023-06-21 | Basf Se | Heterocyclic compounds for the control of invertebrate pests |
EP4198023A1 (en) | 2021-12-16 | 2023-06-21 | Basf Se | Pesticidally active thiosemicarbazone compounds |
AR128372A1 (es) | 2022-01-31 | 2024-04-24 | Pairwise Plants Services Inc | Supresión de la respuesta de evitación de la sombra en las plantas |
WO2023148030A1 (en) | 2022-02-01 | 2023-08-10 | Globachem Nv | Methods and compositions for controlling pests in corn |
WO2023148028A1 (en) | 2022-02-01 | 2023-08-10 | Globachem Nv | Methods and compositions for controlling pests |
WO2023156402A1 (en) | 2022-02-17 | 2023-08-24 | Basf Se | Pesticidally active thiosemicarbazone compounds |
EP4238971A1 (en) | 2022-03-02 | 2023-09-06 | Basf Se | Substituted isoxazoline derivatives |
WO2023168217A1 (en) | 2022-03-02 | 2023-09-07 | Pairwise Plants Services, Inc. | Modification of brassinosteroid receptor genes to improve yield traits |
WO2023192838A1 (en) | 2022-03-31 | 2023-10-05 | Pairwise Plants Services, Inc. | Early flowering rosaceae plants with improved characteristics |
US20230357789A1 (en) | 2022-04-07 | 2023-11-09 | Pairwise Plants Services, Inc. | Methods and compositions for improving resistance to fusarium head blight |
WO2023205714A1 (en) | 2022-04-21 | 2023-10-26 | Pairwise Plants Services, Inc. | Methods and compositions for improving yield traits |
WO2023215704A1 (en) | 2022-05-02 | 2023-11-09 | Pairwise Plants Services, Inc. | Methods and compositions for enhancing yield and disease resistance |
WO2023213626A1 (en) | 2022-05-03 | 2023-11-09 | Bayer Aktiengesellschaft | Use of (5s)-3-[3-(3-chloro-2-fluorophenoxy)-6-methylpyridazin-4-yl]-5-(2-chloro-4-methylbenzyl)-5,6-dihydro-4h-1,2,4-oxadiazine for controlling unwanted microorganisms |
WO2023213670A1 (en) | 2022-05-03 | 2023-11-09 | Bayer Aktiengesellschaft | Crystalline forms of (5s)-3-[3-(3-chloro-2-fluorophenoxy)-6-methylpyridazin-4-yl]-5-(2-chloro-4-methylbenzyl)-5,6-dihydro-4h-1,2,4-oxadiazine |
WO2023215809A1 (en) | 2022-05-05 | 2023-11-09 | Pairwise Plants Services, Inc. | Methods and compositions for modifying root architecture and/or improving plant yield traits |
US20230416771A1 (en) | 2022-06-27 | 2023-12-28 | Pairwise Plants Services, Inc. | Methods and compositions for modifying shade avoidance in plants |
US20240000031A1 (en) | 2022-06-29 | 2024-01-04 | Pairwise Plants Services, Inc. | Methods and compositions for controlling meristem size for crop improvement |
US20240002873A1 (en) | 2022-06-29 | 2024-01-04 | Pairwise Plants Services, Inc. | Methods and compositions for controlling meristem size for crop improvement |
WO2024028243A1 (en) | 2022-08-02 | 2024-02-08 | Basf Se | Pyrazolo pesticidal compounds |
WO2024030984A1 (en) | 2022-08-04 | 2024-02-08 | Pairwise Plants Services, Inc. | Methods and compositions for improving yield traits |
US20240060081A1 (en) | 2022-08-11 | 2024-02-22 | Pairwise Plants Services, Inc. | Methods and compositions for controlling meristem size for crop improvement |
WO2024054880A1 (en) | 2022-09-08 | 2024-03-14 | Pairwise Plants Services, Inc. | Methods and compositions for improving yield characteristics in plants |
EP4342885A1 (en) | 2022-09-20 | 2024-03-27 | Basf Se | N-(3-(aminomethyl)-phenyl)-5-(4-phenyl)-5-(trifluoromethyl)-4,5-dihydroisoxazol-3-amine derivatives and similar compounds as pesticides |
WO2024068520A1 (en) | 2022-09-28 | 2024-04-04 | Bayer Aktiengesellschaft | 3-(hetero)aryl-5-chlorodifluoromethyl-1,2,4-oxadiazole as fungicide |
WO2024068517A1 (en) | 2022-09-28 | 2024-04-04 | Bayer Aktiengesellschaft | 3-(hetero)aryl-5-chlorodifluoromethyl-1,2,4-oxadiazole as fungicide |
WO2024068518A1 (en) | 2022-09-28 | 2024-04-04 | Bayer Aktiengesellschaft | 3-heteroaryl-5-chlorodifluoromethyl-1,2,4-oxadiazole as fungicide |
WO2024068519A1 (en) | 2022-09-28 | 2024-04-04 | Bayer Aktiengesellschaft | 3-(hetero)aryl-5-chlorodifluoromethyl-1,2,4-oxadiazole as fungicide |
EP4295688A1 (en) | 2022-09-28 | 2023-12-27 | Bayer Aktiengesellschaft | Active compound combination |
EP4361126A1 (en) | 2022-10-24 | 2024-05-01 | Basf Se | Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors xv |
WO2024104818A1 (en) | 2022-11-16 | 2024-05-23 | Basf Se | Substituted benzodiazepines as fungicides |
WO2024104822A1 (en) | 2022-11-16 | 2024-05-23 | Basf Se | Substituted tetrahydrobenzodiazepine as fungicides |
WO2024104815A1 (en) | 2022-11-16 | 2024-05-23 | Basf Se | Substituted benzodiazepines as fungicides |
WO2024104823A1 (en) | 2022-11-16 | 2024-05-23 | Basf Se | New substituted tetrahydrobenzoxazepine |
CN116574724B (zh) * | 2023-03-23 | 2023-11-10 | 科稷达隆(北京)生物技术有限公司 | 抗虫耐草甘膦转基因玉米事件kj1003及其检测方法 |
Family Cites Families (48)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ATE93542T1 (de) * | 1984-12-28 | 1993-09-15 | Plant Genetic Systems Nv | Rekombinante dna, die in pflanzliche zellen eingebracht werden kann. |
US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US5338544A (en) * | 1987-04-16 | 1994-08-16 | Ecogen Inc. | CryIIB protein, insecticidal compositions and methods of use thereof |
US5128130A (en) | 1988-01-22 | 1992-07-07 | Mycogen Corporation | Hybrid Bacillus thuringiensis gene, plasmid and transformed Pseudomonas fluorescens |
US5055294A (en) | 1988-03-03 | 1991-10-08 | Mycogen Corporation | Chimeric bacillus thuringiensis crystal protein gene comprising hd-73 and berliner 1715 toxin genes, transformed and expressed in pseudomonas fluorescens |
AU638438B2 (en) | 1989-02-24 | 1993-07-01 | Monsanto Technology Llc | Synthetic plant genes and method for preparation |
DK0426641T3 (da) | 1989-10-31 | 2000-10-23 | Monsanto Co | Promotor til transgene planter |
JPH0648052A (ja) * | 1992-06-01 | 1994-02-22 | Ricoh Co Ltd | 昇華型熱転写記録媒体 |
US5877012A (en) * | 1993-03-25 | 1999-03-02 | Novartis Finance Corporation | Class of proteins for the control of plant pests |
GB9318207D0 (en) | 1993-09-02 | 1993-10-20 | Sandoz Ltd | Improvements in or relating to organic compounds |
US5362865A (en) * | 1993-09-02 | 1994-11-08 | Monsanto Company | Enhanced expression in plants using non-translated leader sequences |
US5689052A (en) * | 1993-12-22 | 1997-11-18 | Monsanto Company | Synthetic DNA sequences having enhanced expression in monocotyledonous plants and method for preparation thereof |
US5545817A (en) | 1994-03-11 | 1996-08-13 | Calgene, Inc. | Enhanced expression in a plant plastid |
US5545818A (en) | 1994-03-11 | 1996-08-13 | Calgene Inc. | Expression of Bacillus thuringiensis cry proteins in plant plastids |
US5593881A (en) | 1994-05-06 | 1997-01-14 | Mycogen Corporation | Bacillus thuringiensis delta-endotoxin |
US5527883A (en) | 1994-05-06 | 1996-06-18 | Mycogen Corporation | Delta-endotoxin expression in pseudomonas fluorescens |
CN1210402C (zh) | 1996-11-20 | 2005-07-13 | 孟山都技术有限公司 | 广谱δ-内毒素 |
US6017534A (en) | 1996-11-20 | 2000-01-25 | Ecogen, Inc. | Hybrid Bacillus thuringiensis δ-endotoxins with novel broad-spectrum insecticidal activity |
EP0950109A1 (en) | 1997-10-31 | 1999-10-20 | Novartis AG | Glyphosate resistant transgenic plants |
US6060594A (en) | 1997-12-18 | 2000-05-09 | Ecogen, Inc. | Nucleic acid segments encoding modified bacillus thuringiensis coleopteran-toxic crystal proteins |
US6572830B1 (en) | 1998-10-09 | 2003-06-03 | Motorola, Inc. | Integrated multilayered microfludic devices and methods for making the same |
US6489542B1 (en) | 1998-11-04 | 2002-12-03 | Monsanto Technology Llc | Methods for transforming plants to express Cry2Ab δ-endotoxins targeted to the plastids |
JP4544748B2 (ja) | 1998-12-01 | 2010-09-15 | ダウ・アグロサイエンス・エル・エル・シー | 植物細胞内に導入された遺伝子の発現を増加するための人工マトリックス付着領域 |
US20100293669A2 (en) * | 1999-05-06 | 2010-11-18 | Jingdong Liu | Nucleic Acid Molecules and Other Molecules Associated with Plants and Uses Thereof for Plant Improvement |
US6603061B1 (en) | 1999-07-29 | 2003-08-05 | Monsanto Company | Agrobacterium-mediated plant transformation method |
WO2002014517A1 (en) | 2000-08-11 | 2002-02-21 | Monsanto Technology Llc | BROAD-SPECTRUM δ-ENDOTOXINS |
CA2419029A1 (en) | 2000-08-25 | 2002-02-28 | Syngenta Participations Ag | Bacillus thuringiensis crystal protein hybrids |
US6713259B2 (en) | 2000-09-13 | 2004-03-30 | Monsanto Technology Llc | Corn event MON810 and compositions and methods for detection thereof |
WO2002094008A2 (en) * | 2001-01-26 | 2002-11-28 | Virginia Tech Intellectual Properties, Inc. | Transgenic plants protected against parasitic plants |
EG26529A (en) | 2001-06-11 | 2014-01-27 | مونسانتو تكنولوجى ل ل سى | Prefixes for detection of DNA molecule in cotton plant MON15985 which gives resistance to damage caused by insect of squamous lepidoptera |
CN1134540C (zh) | 2001-06-22 | 2004-01-14 | 中国科学院微生物研究所 | 一种可使其产物分泌到细胞外的嵌合杀虫蛋白基因 |
EP1402037A1 (en) | 2001-06-22 | 2004-03-31 | Syngenta Participations AG | Plant genes involved in defense against pathogens |
US6868634B2 (en) * | 2001-10-04 | 2005-03-22 | Mississippi State University | Insect resistance management in agricultural applications |
US20030208790A1 (en) | 2002-05-03 | 2003-11-06 | Stefan Jansens | Insect resistant plants and methods for making same |
WO2004020636A1 (en) * | 2002-08-29 | 2004-03-11 | Monsanto Technology, Llc | Nucleotide sequences encoding cry1bb proteins for enhanced expression in plants |
US20040216190A1 (en) | 2003-04-28 | 2004-10-28 | Kovalic David K. | Nucleic acid molecules and other molecules associated with plants and uses thereof for plant improvement |
HUE028375T2 (en) | 2003-05-02 | 2016-12-28 | Dow Agrosciences Llc | TC 1507 corn and a method for detecting it |
MXPA05012482A (es) | 2003-05-20 | 2006-07-03 | Investigen Inc | Sistema para detectar polinucleotidos. |
TWI269809B (en) * | 2003-12-05 | 2007-01-01 | Of Food And Drug Analysis Dept | A plasmid for detection of genetically modified maize |
US7157281B2 (en) * | 2003-12-11 | 2007-01-02 | Monsanto Technology Llc | High lysine maize compositions and event LY038 maize plants |
KR101268896B1 (ko) | 2004-03-25 | 2013-05-30 | 신젠타 파티서페이션즈 아게 | 옥수수 이벤트 mir604 |
US7349043B2 (en) * | 2004-05-24 | 2008-03-25 | Nec Corporation | Light source, display device, portable terminal device, and ray direction switching element |
EP1781771A2 (en) | 2004-08-24 | 2007-05-09 | Nanomix, Inc. | Nanotube sensor devices for dna detection |
US20060068398A1 (en) | 2004-09-24 | 2006-03-30 | Cepheid | Universal and target specific reagent beads for nucleic acid amplification |
US7323556B2 (en) | 2004-09-29 | 2008-01-29 | Pioneer Hi-Bred International, Inc. | Corn event DAS-59122-7 and methods for detection thereof |
WO2007027777A2 (en) * | 2005-08-31 | 2007-03-08 | Monsanto Technology Llc | Nucleotide sequences encoding insecticidal proteins |
BRPI0712921B1 (pt) | 2006-05-26 | 2024-01-30 | Monsanto Technology Llc | Moléculas de dna do evento transgênico mon89034, métodos para detecção do referido evento, produção de plantas transgênicas compreendendo o mesmo, determinar sua zigosi5 dade, proteger uma planta de milho da infestação de insetos, bem como par de moléculas de dna e kit de detecção de dna |
-
2007
- 2007-05-24 BR BRPI0712921-1A patent/BRPI0712921B1/pt active IP Right Grant
- 2007-05-24 NZ NZ573179A patent/NZ573179A/en unknown
- 2007-05-24 EA EA200870576A patent/EA022829B1/ru unknown
- 2007-05-24 SI SI200731514T patent/SI2021476T1/sl unknown
- 2007-05-24 BR BR122016025844-0A patent/BR122016025844B1/pt active IP Right Grant
- 2007-05-24 PT PT77977361T patent/PT2021476E/pt unknown
- 2007-05-24 WO PCT/US2007/069662 patent/WO2007140256A1/en active Application Filing
- 2007-05-24 PL PL07797736T patent/PL2021476T3/pl unknown
- 2007-05-24 KR KR1020137008503A patent/KR101366363B1/ko active IP Right Grant
- 2007-05-24 EP EP07797736.1A patent/EP2021476B1/en active Active
- 2007-05-24 AU AU2007267586A patent/AU2007267586B2/en active Active
- 2007-05-24 SG SG201102918-8A patent/SG171612A1/en unknown
- 2007-05-24 CA CA2653338A patent/CA2653338C/en active Active
- 2007-05-24 JP JP2009513394A patent/JP5513883B2/ja active Active
- 2007-05-24 AR ARP070102259A patent/AR061131A1/es active IP Right Grant
- 2007-05-24 MY MYPI20084790 patent/MY150655A/en unknown
- 2007-05-24 CN CN201710333967.5A patent/CN107119128B/zh active Active
- 2007-05-24 ES ES07797736.1T patent/ES2498976T3/es active Active
- 2007-05-24 RS RS20140528A patent/RS53560B1/en unknown
- 2007-05-24 CN CN200780027882.9A patent/CN101495635B/zh active Active
- 2007-05-24 UA UAA200814980A patent/UA98770C2/ru unknown
- 2007-05-24 AP AP2008004692A patent/AP3797A/en active
- 2007-05-24 MX MX2008015108A patent/MX2008015108A/es active IP Right Grant
- 2007-05-24 KR KR1020087031482A patent/KR20090033840A/ko active Application Filing
- 2007-05-25 TW TW096118812A patent/TWI453412B/zh active
- 2007-05-25 UY UY30370A patent/UY30370A1/es not_active Application Discontinuation
- 2007-05-25 US US11/753,574 patent/US8062840B2/en active Active
- 2007-05-25 PE PE2007000654A patent/PE20080456A1/es active IP Right Grant
- 2007-05-25 DO DO2007000106A patent/DOP2007000106A/es unknown
-
2008
- 2008-11-23 IL IL195451A patent/IL195451A/en active IP Right Grant
- 2008-11-25 ZA ZA200810027A patent/ZA200810027B/xx unknown
- 2008-11-25 EG EG2008111912A patent/EG26119A/en active
- 2008-11-26 NI NI200800312A patent/NI200800312A/es unknown
- 2008-11-26 CR CR10461A patent/CR10461A/es unknown
-
2011
- 2011-05-23 US US13/114,022 patent/US8581047B2/en active Active
-
2013
- 2013-04-19 JP JP2013088193A patent/JP5529322B2/ja active Active
- 2013-11-12 US US14/078,275 patent/US9428765B2/en active Active
-
2014
- 2014-10-03 HR HRP20140951TT patent/HRP20140951T1/hr unknown
-
2015
- 2015-02-05 AR ARP150100332A patent/AR099294A2/es unknown
-
2016
- 2016-08-02 US US15/226,733 patent/US10006046B2/en active Active
-
2017
- 2017-08-24 CL CL2017002157A patent/CL2017002157A1/es unknown
-
2018
- 2018-10-01 UY UY0001037911A patent/UY37911A/es unknown
Also Published As
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP5529322B2 (ja) | トランスジェニック事象mon89034に対応するトウモロコシ植物および種子ならびにそれらを検出および使用するための方法 | |
US11767568B2 (en) | Soybean transgenic event MON87751 and methods for detection and use thereof | |
US8049071B2 (en) | Soybean plant and seed corresponding to transgenic event MON87701 and methods for detection thereof | |
US7179965B2 (en) | Cry1F and Cry1Ac transgenic cotton lines and event-specific identification thereof |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A975 | Report on accelerated examination |
Free format text: JAPANESE INTERMEDIATE CODE: A971005 Effective date: 20130521 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20130528 |
|
A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20130827 |
|
A602 | Written permission of extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A602 Effective date: 20130830 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20130927 |
|
A02 | Decision of refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 20131022 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20140220 |
|
A911 | Transfer to examiner for re-examination before appeal (zenchi) |
Free format text: JAPANESE INTERMEDIATE CODE: A911 Effective date: 20140227 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20140318 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20140416 |
|
R150 | Certificate of patent or registration of utility model |
Ref document number: 5529322 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |