JP5523327B2 - 統合型マイクロ流体デバイスおよび方法 - Google Patents

統合型マイクロ流体デバイスおよび方法 Download PDF

Info

Publication number
JP5523327B2
JP5523327B2 JP2010529130A JP2010529130A JP5523327B2 JP 5523327 B2 JP5523327 B2 JP 5523327B2 JP 2010529130 A JP2010529130 A JP 2010529130A JP 2010529130 A JP2010529130 A JP 2010529130A JP 5523327 B2 JP5523327 B2 JP 5523327B2
Authority
JP
Japan
Prior art keywords
nucleic acid
microfluidic device
sample
reservoir
region
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2010529130A
Other languages
English (en)
Japanese (ja)
Other versions
JP2011501665A5 (enExample
JP2011501665A (ja
Inventor
チョウ，パン
シー． ヤング，リンカーン
ロスウェチ，トッド
スピッズ，グウェンドリン
チェン，ツォンユアン
ダブリュ． トーマス，ベンジャミン
リー，トラヴィス
Original Assignee
レオニックス，インコーポレイテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by レオニックス，インコーポレイテッド filed Critical レオニックス，インコーポレイテッド
Publication of JP2011501665A publication Critical patent/JP2011501665A/ja
Publication of JP2011501665A5 publication Critical patent/JP2011501665A5/ja
Application granted granted Critical
Publication of JP5523327B2 publication Critical patent/JP5523327B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F25/00—Flow mixers; Mixers for falling materials, e.g. solid particles
    • B01F25/40—Static mixers
    • B01F25/45—Mixers in which the materials to be mixed are pressed together through orifices or interstitial spaces, e.g. between beads
    • B01F25/451—Mixers in which the materials to be mixed are pressed together through orifices or interstitial spaces, e.g. between beads characterised by means for moving the materials to be mixed or the mixture
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F25/00—Flow mixers; Mixers for falling materials, e.g. solid particles
    • B01F25/40—Static mixers
    • B01F25/45—Mixers in which the materials to be mixed are pressed together through orifices or interstitial spaces, e.g. between beads
    • B01F25/452—Mixers in which the materials to be mixed are pressed together through orifices or interstitial spaces, e.g. between beads characterised by elements provided with orifices or interstitial spaces
    • B01F25/4521—Mixers in which the materials to be mixed are pressed together through orifices or interstitial spaces, e.g. between beads characterised by elements provided with orifices or interstitial spaces the components being pressed through orifices in elements, e.g. flat plates or cylinders, which obstruct the whole diameter of the tube
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/65—Mixers with shaking, oscillating, or vibrating mechanisms the materials to be mixed being directly submitted to a pulsating movement, e.g. by means of an oscillating piston or air column
    • B01F31/651—Mixing by successively aspirating a part of the mixture in a conduit, e.g. a piston, and reinjecting it through the same conduit into the receptacle
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0621—Control of the sequence of chambers filled or emptied
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1816—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using induction heating
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1822—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using Peltier elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1838—Means for temperature control using fluid heat transfer medium
    • B01L2300/1844—Means for temperature control using fluid heat transfer medium using fans
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1838—Means for temperature control using fluid heat transfer medium
    • B01L2300/185—Means for temperature control using fluid heat transfer medium using a liquid as fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B01L2400/0638—Valves, specific forms thereof with moving parts membrane valves, flap valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples

Landscapes

  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
JP2010529130A 2007-10-12 2008-10-10 統合型マイクロ流体デバイスおよび方法 Active JP5523327B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US97951507P 2007-10-12 2007-10-12
US60/979,515 2007-10-12
PCT/US2008/079659 WO2009049268A1 (en) 2007-10-12 2008-10-10 Integrated microfluidic device and methods

Publications (3)

Publication Number Publication Date
JP2011501665A JP2011501665A (ja) 2011-01-13
JP2011501665A5 JP2011501665A5 (enExample) 2011-11-24
JP5523327B2 true JP5523327B2 (ja) 2014-06-18

Family

ID=40251703

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2010529130A Active JP5523327B2 (ja) 2007-10-12 2008-10-10 統合型マイクロ流体デバイスおよび方法

Country Status (5)

Country Link
US (1) US9132398B2 (enExample)
EP (1) EP2265375A1 (enExample)
JP (1) JP5523327B2 (enExample)
CN (1) CN101903104B (enExample)
WO (1) WO2009049268A1 (enExample)

Families Citing this family (76)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2137523A1 (en) 2007-04-04 2009-12-30 Network Biosystems, Inc. Plastic microfluidic separation and detection platforms
WO2010147654A2 (en) 2009-06-15 2010-12-23 Netbio Inc. Improved methods for forensic dna quantitation
IN2012DN03430A (enExample) * 2009-09-21 2015-10-23 Akonni Biosystems
AU2011221244B2 (en) * 2010-02-23 2014-02-13 Rheonix, Inc. Self-contained biological assay apparatus, methods, and applications
US8720036B2 (en) 2010-03-09 2014-05-13 Netbio, Inc. Unitary biochip providing sample-in to results-out processing and methods of manufacture
EP4124856A1 (en) * 2010-03-09 2023-02-01 ANDE Corporation Biochip with valve
JP5786295B2 (ja) * 2010-06-22 2015-09-30 ソニー株式会社 核酸等温増幅反応用マイクロチップ及びその製造方法並びに核酸等温増幅方法
GB2481425A (en) 2010-06-23 2011-12-28 Iti Scotland Ltd Method and device for assembling polynucleic acid sequences
WO2012005717A2 (en) * 2010-06-29 2012-01-12 Analogic Corporation Sample carrier
CN103210079B (zh) * 2010-08-02 2015-07-22 B·L·韦特 用于聚合酶链式反应的可增压筒
FR2967362B1 (fr) 2010-11-16 2012-12-21 Genewave Cartouche microfluidique pour diagnostic moleculaire
CA2824404C (en) 2011-01-06 2023-01-03 Meso Scale Technologies, Llc Assay cartridges for pcr analysis and methods of use thereof
AU2012220825B2 (en) * 2011-02-21 2015-12-17 Rheonix, Inc. Microfluidic device-based nucleic acid purification method
JP2014533952A (ja) 2011-11-17 2014-12-18 リーアニクス・インコーポレイテッドＲｈｅｏｎｉｘ， Ｉｎｃ． 微小流体装置、方法および応用
JP5892589B2 (ja) * 2011-11-24 2016-03-23 マイクロ化学技研株式会社 マイクロデバイス及びバイオアッセイシステム
TW201326814A (zh) * 2011-12-21 2013-07-01 Nat Univ Tsing Hua 篩選細胞適合體之微流體晶片裝置及其方法
US9322054B2 (en) * 2012-02-22 2016-04-26 Lockheed Martin Corporation Microfluidic cartridge
WO2014083496A1 (en) 2012-11-29 2014-06-05 Koninklijke Philips N.V. Cartridge for uptake and processing of a sample
KR102190198B1 (ko) * 2013-02-08 2020-12-14 10엑스 제노믹스, 인크. 폴리뉴클레오티드 바코드 생성
US9857370B2 (en) * 2013-07-22 2018-01-02 National Technology & Engineering Solutions Of Sandia, Llc Amplification of biological targets via on-chip culture for biosensing
GB2516666B (en) * 2013-07-29 2015-09-09 Atlas Genetics Ltd Fluidic cartridge for nucleic acid amplification and detection
EP2878375A1 (en) * 2013-11-29 2015-06-03 Genewave Microfluidic cartridge for molecular diagnosis, docking station using such a microfluidic cartridge, and process for analyzing a biological sample
WO2015138343A1 (en) 2014-03-10 2015-09-17 Click Diagnostics, Inc. Cartridge-based thermocycler
CN106414704B (zh) * 2014-03-14 2020-11-03 生命技术公司 用于核酸扩增和检测的集成系统
WO2016021158A1 (ja) * 2014-08-08 2016-02-11 パナソニックＩｐマネジメント株式会社 核酸増幅デバイス
ES2898103T3 (es) 2014-12-31 2022-03-03 Visby Medical Inc Dispositivos para pruebas de diagnóstico molecular
WO2017004250A1 (en) 2015-06-29 2017-01-05 Arizona Board Of Regents, A Body Corporate Of The State Of Arizona, Acting For And On Behalf Of Arizona State University Systems and methods for continuous flow digital droplet polymerase chain reaction bioanalysis
AU2016331215B2 (en) * 2015-10-02 2022-08-11 The Board Of Trustees Of The Leland Stanford Junior University Sorting biological and non-biological moieties using magnetic levitation
CN105214744A (zh) * 2015-10-26 2016-01-06 深圳华迈兴微医疗科技有限公司 一种磁微粒化学发光微流控芯片
WO2017197040A1 (en) 2016-05-11 2017-11-16 Click Diagnostics, Inc. Devices and methods for nucleic acid extraction
JP6705502B2 (ja) * 2016-06-06 2020-06-03 株式会社ニコン 流体デバイス、システム、精製方法および検出方法
EP3467515A4 (en) * 2016-06-06 2020-04-29 Nikon Corporation FLUID DEVICE, SYSTEM, SAMPLE SUBSTANCE DETECTION METHOD, AND SAMPLE SUBSTANCE PURIFICATION METHOD
EP3478857A1 (en) 2016-06-29 2019-05-08 Click Diagnostics, Inc. Devices and methods for the detection of molecules using a flow cell
CN107198341B (zh) * 2016-09-26 2025-08-29 湖南泰克斯医疗科技有限公司 美容液可控面部美容器
EP3523048B1 (en) * 2016-10-06 2025-03-19 The Regents of The University of California Volumetric micro-injector for capillary electrophoresis
JP2020500517A (ja) 2016-11-28 2020-01-16 アリゾナ ボード オブ リージェンツ オン ビハーフ オブ アリゾナ ステート ユニバーシティ 連続流小滴反応に関連するシステムおよび方法
CN107384776A (zh) * 2017-08-04 2017-11-24 深圳市合川医疗科技有限公司 微流控芯片
CN111655866A (zh) 2017-11-09 2020-09-11 维斯比医学公司 便携式分子诊断装置和检测靶病毒的方法
CN109957492A (zh) * 2017-12-26 2019-07-02 安诺优达基因科技(北京)有限公司 一种用于二代测序dna文库构建的自动化液体处理工作站
US11224873B2 (en) * 2018-03-15 2022-01-18 Kryptos Biotechnologies, Inc. Microfluidic system incorporating light absorbing materials
US11931738B2 (en) 2018-04-24 2024-03-19 Hewlett-Packard Development Company, L.P. Sequenced droplet ejection to deliver fluids
US11925932B2 (en) 2018-04-24 2024-03-12 Hewlett-Packard Development Company, L.P. Microfluidic devices
CN110452806B (zh) * 2018-05-08 2023-03-31 国家纳米科学中心 基于环介导等温扩增的全自动化微流控核酸检测芯片、其试剂盒及方法
CN108722505A (zh) * 2018-05-11 2018-11-02 石家庄禾柏生物技术股份有限公司 一种三明治结构的试剂盘
CN108660074B (zh) * 2018-05-28 2022-04-29 嘉兴市艾科诺生物科技有限公司 核酸提取pcr扩增检测一体化解决方案
US11547993B2 (en) 2018-07-17 2023-01-10 Hewlett-Packard Development Company, L.P. Droplet ejectors with target media
US11325380B2 (en) 2018-07-17 2022-05-10 Hewlett-Packard Development Company, L.P. Droplet ejectors to provide fluids to droplet ejectors
KR101996617B1 (ko) * 2018-10-11 2019-07-04 주식회사 엘지화학 일체형 카트리지
CN111057638B (zh) * 2018-10-17 2023-06-27 北京致雨生物科技有限公司 样本处理装置及方法，以及包括该处理装置的数字pcr系统
GB201819417D0 (en) * 2018-11-29 2019-01-16 Quantumdx Group Ltd Vacuum-assisted drying of filters in microfluidic systems
EP3807007A4 (en) * 2018-11-30 2022-05-04 Illumina Inc SYSTEMS AND METHODS FOR ACTIVE HEATING OF A CARTRIDGE
WO2020174918A1 (ja) * 2019-02-27 2020-09-03 Ｐｈｃホールディングス株式会社 核酸増幅装置
CN110331089B (zh) * 2019-05-21 2023-07-21 四川迪亚生物科技集团有限公司 一种全自动核酸提取扩增检测微流控芯片盒及其应用
CN110257240B (zh) * 2019-07-16 2024-04-09 深圳博识诊断技术有限公司 核酸提取、扩增及检测一体试剂卡
US11008627B2 (en) 2019-08-15 2021-05-18 Talis Biomedical Corporation Diagnostic system
WO2021056005A1 (en) 2019-09-20 2021-03-25 Siemens Healthcare Diagnostics Inc. Sample preparation apparatus and multi-well plate with pcr chip
WO2021188580A1 (en) * 2020-03-17 2021-09-23 Detect, Inc. Rapid diagnostic test
US20230227888A1 (en) * 2020-05-12 2023-07-20 Godx, Inc. Point of need diagnostic device and methods of use thereof
CN111690510B (zh) * 2020-06-11 2023-07-21 上海前瞻创新研究院有限公司 一种致病菌检测反应器及其制备和应用方法
WO2022061521A1 (zh) * 2020-09-22 2022-03-31 京东方科技集团股份有限公司 核酸提取微流控芯片、核酸提取装置及提取方法
EP3978119A1 (en) 2020-09-30 2022-04-06 Levitas, Inc. Particle separator system, materials, and methods of use
CN116888273A (zh) * 2020-10-19 2023-10-13 富默乐国际控股有限公司 用于样本至结果pcr分析的具有流体通道几何形状的装置及其使用方法
US12371738B2 (en) * 2020-10-26 2025-07-29 Veritas Diagnostic Llc Microfluidic platform and a method for electrostatically-enhanced, excitation-modulated reverse transcriptase quantitative polymerase chain reaction analysis
CN114471751B (zh) * 2020-10-27 2024-12-10 京东方科技集团股份有限公司 检测芯片及其操作方法、检测装置
WO2022117053A1 (en) * 2020-12-02 2022-06-09 Suzhou Singleron Biotechnologies Co., Ltd. Reagent exchange methods, devices, and systems
KR20230169935A (ko) 2021-01-12 2023-12-18 데피니티브 바이오테크놀로지스 엘엘씨 생물학적 시료들에서 핵산을 검출하기 위한 디바이스및 방법
CN114763511B (zh) * 2021-01-14 2025-09-05 苏州思纳福医疗科技有限公司 物料转移机构、检测盒、核酸检测设备及物料转移方法
CN113145185B (zh) * 2021-02-08 2022-11-01 北京理工大学 一种模块化的微流控核酸检测芯片及系统
CN114733587B (zh) * 2022-04-01 2024-02-20 合肥诺迈基生物科技有限公司 荧光检测芯片、荧光检测系统、荧光检测方法及其应用
KR20250005312A (ko) * 2022-04-15 2025-01-09 큐리어터, 아이엔씨. 라운딩된 모서리를 구비한 마이크로유체 장치
CN115322888B (zh) * 2022-09-14 2024-01-19 翊新诊断技术(苏州)有限公司 微流控囊袋式循环pcr芯片及其应用
CN115353968B (zh) * 2022-10-20 2023-02-03 湖南冠牧生物科技有限公司 一种快速核酸检测微流控芯片、核酸检测系统及方法
CN116179287A (zh) * 2022-12-08 2023-05-30 南京仁为医药科技有限公司 一种利用连续流微通道反应装置制备的快速高效多酶清洗液
CN118685257B (zh) * 2023-04-26 2025-09-02 翊新诊断技术(苏州)有限公司 一种基于硅膜法的全集成式核酸检测芯片
CN118360128B (zh) * 2024-05-24 2025-09-16 大连大学 一种核酸扩增微流控芯片及使用方法
EP4721858A1 (en) * 2024-10-02 2026-04-08 Sartorius Lab Instruments GmbH & Co. KG Nucleic acid contamination-free cartridge for sterility testing

Family Cites Families (89)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5038852A (en) 1986-02-25 1991-08-13 Cetus Corporation Apparatus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
US5188963A (en) 1989-11-17 1993-02-23 Gene Tec Corporation Device for processing biological specimens for analysis of nucleic acids
CA1339731C (en) 1988-10-12 1998-03-17 Charles T. Caskey Multiplex genomic dna amplification for deletion detection
US5234809A (en) 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
US5427930A (en) 1990-01-26 1995-06-27 Abbott Laboratories Amplification of target nucleic acids using gap filling ligase chain reaction
US6787338B2 (en) 1990-06-04 2004-09-07 The University Of Utah Method for rapid thermal cycling of biological samples
US5935522A (en) 1990-06-04 1999-08-10 University Of Utah Research Foundation On-line DNA analysis system with rapid thermal cycling
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US5270183A (en) 1991-02-08 1993-12-14 Beckman Research Institute Of The City Of Hope Device and method for the automated cycling of solutions between two or more temperatures
US7297313B1 (en) 1991-08-31 2007-11-20 The Regents Of The University Of California Microfabricated reactor, process for manufacturing the reactor, and method of amplification
US5587128A (en) * 1992-05-01 1996-12-24 The Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification devices
US5726026A (en) 1992-05-01 1998-03-10 Trustees Of The University Of Pennsylvania Mesoscale sample preparation device and systems for determination and processing of analytes
US5498392A (en) 1992-05-01 1996-03-12 Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification device and method
WO1994003624A1 (en) 1992-08-04 1994-02-17 Auerbach Jeffrey I Methods for the isothermal amplification of nucleic acid molecules
DE69429038T2 (de) 1993-07-28 2002-03-21 Pe Corporation (Ny), Norwalk Vorrichtung und Verfahren zur Nukleinsäurevervielfältigung
US5639428A (en) 1994-07-19 1997-06-17 Becton Dickinson And Company Method and apparatus for fully automated nucleic acid amplification, nucleic acid assay and immunoassay
DE4435107C1 (de) 1994-09-30 1996-04-04 Biometra Biomedizinische Analy Miniaturisierter Fluß-Thermocycler
AU6541596A (en) 1995-06-16 1997-01-15 University Of Washington Microfabricated differential extraction device and method
US5716852A (en) 1996-03-29 1998-02-10 University Of Washington Microfabricated diffusion-based chemical sensor
DE69619400T2 (de) 1995-06-16 2002-09-26 Univ Washington Seattle Flacher mikrogefertigter querstromfilter für flüssigkeiten
US5856174A (en) 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
US20050100946A1 (en) 1995-06-29 2005-05-12 Affymetrix, Inc. Integrated nucleic acid diagnostic device and method for in-situ confocal microscopy
US6168948B1 (en) 1995-06-29 2001-01-02 Affymetrix, Inc. Miniaturized genetic analysis systems and methods
US20020022261A1 (en) 1995-06-29 2002-02-21 Anderson Rolfe C. Miniaturized genetic analysis systems and methods
US5724404A (en) 1995-07-03 1998-03-03 Garcia; Max Integrated international telephone circuit monitoring system
US5989813A (en) 1995-07-13 1999-11-23 Molecular Innovations, Inc. Detection of amplified nucleic acid sequences using bifunctional haptenization and dyed microparticles
US5726751A (en) 1995-09-27 1998-03-10 University Of Washington Silicon microchannel optical flow cytometer
US5863502A (en) 1996-01-24 1999-01-26 Sarnoff Corporation Parallel reaction cassette and associated devices
US5741647A (en) 1996-02-16 1998-04-21 Tam; Joseph Wing On Flow through nucleic acid hybridisation uses thereof and a device thereof
US5747349A (en) 1996-03-20 1998-05-05 University Of Washington Fluorescent reporter beads for fluid analysis
US5948684A (en) 1997-03-31 1999-09-07 University Of Washington Simultaneous analyte determination and reference balancing in reference T-sensor devices
DE69728269T2 (de) 1996-06-14 2005-03-10 University Of Washington, Seattle Absorbtionsverbessertes differentielles extraktionsverfahren
US5748827A (en) 1996-10-23 1998-05-05 University Of Washington Two-stage kinematic mount
US5974867A (en) 1997-06-13 1999-11-02 University Of Washington Method for determining concentration of a laminar sample stream
US5932799A (en) 1997-07-21 1999-08-03 Ysi Incorporated Microfluidic analyzer module
US5965410A (en) 1997-09-02 1999-10-12 Caliper Technologies Corp. Electrical current for controlling fluid parameters in microchannels
US6007775A (en) 1997-09-26 1999-12-28 University Of Washington Multiple analyte diffusion based chemical sensor
US6953675B2 (en) 1997-11-06 2005-10-11 Immunomedics, Inc. Landscaped antibodies and antibody fragments for clinical use
US6210882B1 (en) 1998-01-29 2001-04-03 Mayo Foundation For Medical Education And Reseach Rapid thermocycling for sample analysis
ES2322859T3 (es) 1998-05-01 2009-06-30 Gen-Probe Incorporated Analizador de diagnostico automatizado.
WO1999064836A1 (en) 1998-06-08 1999-12-16 Caliper Technologies Corp. Microfluidic devices, systems and methods for performing integrated reactions and separations
WO2000023190A1 (fr) 1998-10-16 2000-04-27 Commissariat A L'energie Atomique Dispositif d'analyse chimique et/ou biochimique avec un support d'analyse
US6171850B1 (en) 1999-03-08 2001-01-09 Caliper Technologies Corp. Integrated devices and systems for performing temperature controlled reactions and analyses
US6303343B1 (en) 1999-04-06 2001-10-16 Caliper Technologies Corp. Inefficient fast PCR
GB9922971D0 (en) 1999-09-29 1999-12-01 Secr Defence Reaction system
WO2001026813A2 (en) 1999-10-08 2001-04-19 Micronics, Inc. Microfluidics without electrically of mechanically operated pumps
EP1263533B1 (en) 2000-03-14 2010-03-03 Micronics, Inc. Microfluidic analysis cartridge
US20010048900A1 (en) 2000-05-24 2001-12-06 Bardell Ronald L. Jet vortex mixer
US7087414B2 (en) 2000-06-06 2006-08-08 Applera Corporation Methods and devices for multiplexing amplification reactions
US6627159B1 (en) 2000-06-28 2003-09-30 3M Innovative Properties Company Centrifugal filling of sample processing devices
WO2002022878A1 (en) 2000-09-14 2002-03-21 Caliper Technologies Corp. Microfluidic devices and methods for performing temperature mediated reactions
EP1334279A1 (en) 2000-11-06 2003-08-13 Nanostream, Inc. Uni-directional flow microfluidic components
WO2002055198A2 (en) 2000-11-06 2002-07-18 Nanostream Inc Microfluidic flow control devices
US6521188B1 (en) 2000-11-22 2003-02-18 Industrial Technology Research Institute Microfluidic actuator
US20020098122A1 (en) 2001-01-22 2002-07-25 Angad Singh Active disposable microfluidic system with externally actuated micropump
US6576459B2 (en) 2001-03-23 2003-06-10 The Regents Of The University Of California Sample preparation and detection device for infectious agents
EP1384022A4 (en) 2001-04-06 2004-08-04 California Inst Of Techn NUCLEIC ACID AMPLIFICATION USING MICROFLUID DEVICES
US6802342B2 (en) 2001-04-06 2004-10-12 Fluidigm Corporation Microfabricated fluidic circuit elements and applications
US20020155010A1 (en) 2001-04-24 2002-10-24 Karp Christoph D. Microfluidic valve with partially restrained element
US20030032172A1 (en) 2001-07-06 2003-02-13 The Regents Of The University Of California Automated nucleic acid assay system
US7025323B2 (en) 2001-09-21 2006-04-11 The Regents Of The University Of California Low power integrated pumping and valving arrays for microfluidic systems
US7691333B2 (en) 2001-11-30 2010-04-06 Fluidigm Corporation Microfluidic device and methods of using same
US7223371B2 (en) 2002-03-14 2007-05-29 Micronics, Inc. Microfluidic channel network device
US6843263B2 (en) 2002-06-24 2005-01-18 Industrial Technology Research Institute Partially closed microfluidic system and microfluidic driving method
ITTO20020808A1 (it) 2002-09-17 2004-03-18 St Microelectronics Srl Dispositivo integrato di analisi del dna.
US20040086872A1 (en) * 2002-10-31 2004-05-06 Childers Winthrop D. Microfluidic system for analysis of nucleic acids
US20040110132A1 (en) 2002-12-06 2004-06-10 Affymetrix, Inc. Method for concentrate nucleic acids
WO2004061085A2 (en) 2002-12-30 2004-07-22 The Regents Of The University Of California Methods and apparatus for pathogen detection and analysis
US7122153B2 (en) 2003-01-08 2006-10-17 Ho Winston Z Self-contained microfluidic biochip and apparatus
AU2004205671A1 (en) 2003-01-21 2004-08-05 Perkinelmer Health Sciences, Inc. Method and system for microfluidic manipulation, amplification and analysis of fluids, for example, bacteria assays and antiglobulin testing
TWM255251U (en) 2003-12-10 2005-01-11 Dau-Ming Guo Ozone garbage bin
CN1922317A (zh) * 2003-12-24 2007-02-28 3M创新有限公司 使用微流体装置和浓缩步骤的核酸分离用方法和试剂盒
CN102759466A (zh) 2004-09-15 2012-10-31 英特基因有限公司 微流体装置
US7832429B2 (en) * 2004-10-13 2010-11-16 Rheonix, Inc. Microfluidic pump and valve structures and fabrication methods
US20060184038A1 (en) * 2004-11-30 2006-08-17 Affymetrix, Inc. System, method, and product for analyzing images comprising small feature sizes
US20070042427A1 (en) 2005-05-03 2007-02-22 Micronics, Inc. Microfluidic laminar flow detection strip
KR20070052958A (ko) 2005-11-18 2007-05-23 주식회사 엘지생명과학 Ｏｎ－ｃｈｉｐ 폴리머 밸브를 가진 ＰＣＲ용 플라스틱 칩
US7976795B2 (en) * 2006-01-19 2011-07-12 Rheonix, Inc. Microfluidic systems
WO2008030631A2 (en) 2006-02-03 2008-03-13 Microchip Biotechnologies, Inc. Microfluidic devices
JP5254949B2 (ja) 2006-03-15 2013-08-07 マイクロニクス， インコーポレイテッド 一体型の核酸アッセイ
US7766033B2 (en) 2006-03-22 2010-08-03 The Regents Of The University Of California Multiplexed latching valves for microfluidic devices and processors
US20100216657A1 (en) 2006-05-16 2010-08-26 Arcxis Biotechnologies, Inc. Pcr-free sample preparation and detection systems for high speed biologic analysis and identification
US8383323B2 (en) 2006-09-13 2013-02-26 Samsung Austin Semiconductor, L.P. Selective imaging through dual photoresist layers
WO2008147382A1 (en) * 2006-09-27 2008-12-04 Micronics, Inc. Integrated microfluidic assay devices and methods
JP5382347B2 (ja) 2006-10-11 2014-01-08 フルイディウム コーポレーション 使い捨て可能なマイクロ精製カード、方法、およびそのシステム

Also Published As

Publication number Publication date
WO2009049268A1 (en) 2009-04-16
JP2011501665A (ja) 2011-01-13
CN101903104A (zh) 2010-12-01
EP2265375A1 (en) 2010-12-29
CN101903104B (zh) 2014-06-25
US9132398B2 (en) 2015-09-15
US20120115738A1 (en) 2012-05-10

Similar Documents

Publication Publication Date Title
US9132398B2 (en) Integrated microfluidic device and methods
Wu et al. Extraction, amplification and detection of DNA in microfluidic chip-based assays
Yin et al. Integrated microfluidic systems with sample preparation and nucleic acid amplification
CN101848765B (zh) 分析方法
EP3132073B1 (en) Portable nucleic acid analysis system and high-performance microfluidic electroactive polymer actuators
Zhang et al. Single-molecule DNA amplification and analysis using microfluidics
US6326211B1 (en) Method of manipulating a gas bubble in a microfluidic device
CN100503838C (zh) 用于分析核酸的微流系统
JP3909859B2 (ja) 中規模ポリヌクレオチド増幅装置
US20020022261A1 (en) Miniaturized genetic analysis systems and methods
US20120028311A1 (en) Cartridge with lysis chamber and droplet generator
JP2018029597A (ja) 結合要素を用いたアッセイのための装置及び方法
KR20110030415A (ko) 만능 샘플 제조 시스템 및 집적 분석 시스템에서의 용도
CN107513495B (zh) 用于核酸检测的多通道微滴检测芯片
CN1732044A (zh) 用于核酸分析的显微流体系统
McCalla et al. Microfluidic reactors for diagnostics applications
US20050100946A1 (en) Integrated nucleic acid diagnostic device and method for in-situ confocal microscopy
Phaneuf et al. Sensitive, microliter PCR with consensus degenerate primers for Epstein Barr virus amplification
CN119793555B (zh) 一种便携微流控芯片、检测方法及检测装置
Becker et al. Continuous-flow PCR using segmented flow and integrating sample preparation
Tai et al. An integrated microfluidic system capable of sample pretreatment and hybridization for microarrays

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20111005

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20111005

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20130820

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20131118

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20131125

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20131219

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20131227

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20140117

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20140325

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20140408

R150 Certificate of patent or registration of utility model

Ref document number: 5523327

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250