JP5484879B2 - 超解像顕微鏡 - Google Patents

超解像顕微鏡 Download PDF

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Publication number
JP5484879B2
JP5484879B2 JP2009281179A JP2009281179A JP5484879B2 JP 5484879 B2 JP5484879 B2 JP 5484879B2 JP 2009281179 A JP2009281179 A JP 2009281179A JP 2009281179 A JP2009281179 A JP 2009281179A JP 5484879 B2 JP5484879 B2 JP 5484879B2
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JP
Japan
Prior art keywords
light
illumination light
super
polarization
control element
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Expired - Fee Related
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JP2009281179A
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English (en)
Japanese (ja)
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JP2011123314A (ja
JP2011123314A5 (https=
Inventor
慶記 池滝
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Olympus Corp
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Olympus Corp
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Priority to JP2009281179A priority Critical patent/JP5484879B2/ja
Priority to US12/961,917 priority patent/US8536543B2/en
Priority to CN201010580742.8A priority patent/CN102096180B/zh
Publication of JP2011123314A publication Critical patent/JP2011123314A/ja
Publication of JP2011123314A5 publication Critical patent/JP2011123314A5/ja
Application granted granted Critical
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Expired - Fee Related legal-status Critical Current
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    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0068—Optical details of the image generation arrangements using polarisation
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0072—Optical details of the image generation details concerning resolution or correction, including general design of CSOM objectives
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/58—Optics for apodization or superresolution; Optical synthetic aperture systems

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  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Microscoopes, Condenser (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Polarising Elements (AREA)
JP2009281179A 2009-12-11 2009-12-11 超解像顕微鏡 Expired - Fee Related JP5484879B2 (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2009281179A JP5484879B2 (ja) 2009-12-11 2009-12-11 超解像顕微鏡
US12/961,917 US8536543B2 (en) 2009-12-11 2010-12-07 Super-resolution microscope
CN201010580742.8A CN102096180B (zh) 2009-12-11 2010-12-09 超分辨率显微镜

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2009281179A JP5484879B2 (ja) 2009-12-11 2009-12-11 超解像顕微鏡

Publications (3)

Publication Number Publication Date
JP2011123314A JP2011123314A (ja) 2011-06-23
JP2011123314A5 JP2011123314A5 (https=) 2013-06-20
JP5484879B2 true JP5484879B2 (ja) 2014-05-07

Family

ID=44129343

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2009281179A Expired - Fee Related JP5484879B2 (ja) 2009-12-11 2009-12-11 超解像顕微鏡

Country Status (3)

Country Link
US (1) US8536543B2 (https=)
JP (1) JP5484879B2 (https=)
CN (1) CN102096180B (https=)

Families Citing this family (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7586583B2 (en) * 2005-09-15 2009-09-08 Franklin Mark Schellenberg Nanolithography system
GB0907965D0 (en) * 2009-05-08 2009-06-24 King S College London Imaging system
JP5703126B2 (ja) * 2010-09-30 2015-04-15 富士フイルム株式会社 生体分子検出装置および生体分子検出方法
DE102011013613A1 (de) * 2010-10-01 2012-04-05 Carl Zeiss Microimaging Gmbh Mikroskop und Mikroskopierverfahren
CN102866492A (zh) * 2011-07-06 2013-01-09 黄书伟 平衡检测共聚焦显微镜成像系统及其三维影像重建方法
US9866379B2 (en) * 2011-09-30 2018-01-09 Los Alamos National Security, Llc Polarization tracking system for free-space optical communication, including quantum communication
JP6255389B2 (ja) 2012-05-07 2017-12-27 アンセルム（アンスティチュ ナシオナル ドゥ ラ サンテ エ ドゥ ラ ルシェルシュ メディカル） 試料中の関心組織を高空間分解能で画像化するための顕微鏡
CN102735617B (zh) * 2012-06-29 2014-06-04 浙江大学 一种超分辨显微方法和装置
CA2882288C (en) 2012-08-17 2020-10-27 Los Alamos National Security, Llc Quantum communications system with integrated photonic devices
CN103048299B (zh) * 2012-11-26 2015-01-21 浙江大学 基于荧光寿命差分的超分辨显微方法和装置
WO2014087624A1 (ja) 2012-12-05 2014-06-12 パナソニック株式会社 画像生成装置及び画像生成方法
JP2014182239A (ja) 2013-03-19 2014-09-29 Olympus Corp 超解像顕微鏡
US9971136B2 (en) * 2013-03-21 2018-05-15 ETH Zürich Method and device to achieve spatially confined photointeraction at the focal volume of a microscope
JP6234105B2 (ja) * 2013-08-05 2017-11-22 オリンパス株式会社 超解像顕微鏡
CN103592278B (zh) * 2013-11-21 2016-01-20 中国计量学院 基于荧光发射抑制机理的随机定位超分辨显微方法及装置
JP6278707B2 (ja) * 2014-01-09 2018-02-14 オリンパス株式会社 光学装置
GB2541308B (en) * 2014-05-29 2021-05-19 Hitachi High Tech Corp Microscope observation container and observation device
CN104062750B (zh) * 2014-06-18 2016-07-06 浙江大学 一种双光子荧光受激发射微分超分辨率显微方法与装置
CN104515760B (zh) 2014-12-17 2017-10-31 深圳市纳观生物有限公司 双色荧光定位超分辨率生物显微方法及系统
TWI563288B (en) * 2015-03-03 2016-12-21 Southport Corp Optical image scanning component and microscope device
GB201515862D0 (en) * 2015-09-08 2015-10-21 Univ Southampton Polarisation microscope
US9645376B1 (en) * 2015-10-14 2017-05-09 Abberior Instruments Gmbh Scanner head and device with scanner head
CN105242408A (zh) * 2015-11-13 2016-01-13 上海理工大学 一种超分辨光学管道的生成方法
CN105973853B (zh) * 2016-05-10 2018-11-09 浙江大学 一种基于双模式竞争激发的超分辨显微方法和装置
CN107783267B (zh) * 2016-08-30 2024-04-26 北京大学 显微放大系统
US10852519B2 (en) * 2016-11-30 2020-12-01 Asm Technology Singapore Pte Ltd Confocal imaging of an object utilising a pinhole array
JP2018120006A (ja) * 2017-01-23 2018-08-02 オリンパス株式会社 超解像顕微鏡
CN107607203B (zh) * 2017-09-08 2019-08-06 武汉大学 基于结构相似度的显著性波段选择方法
JP2019091029A (ja) * 2017-11-10 2019-06-13 住友化学株式会社 複合位相差板、光学積層体、及び画像表示装置
CN108254340B (zh) * 2017-12-28 2021-11-16 苏州国科医工科技发展(集团)有限公司 基于线偏振调制的扫描显微镜
CN109211854B (zh) * 2018-08-10 2021-07-02 国家纳米科学中心 多光束多光子重扫描显微成像装置
DE102019101777B4 (de) * 2019-01-24 2023-11-02 Carl Zeiss Meditec Ag Mikroskopieverfahren
DE102019102330C5 (de) 2019-01-30 2023-02-23 Leica Microsystems Cms Gmbh Optisches System für ein Mikroskop, Mikroskop mit einem optischen System und Verfahren zur Abbildung eines Objekts unter Verwendung eines Mikroskops
CN111443496B (zh) * 2020-03-26 2021-12-14 上海理工大学 一种实现双光束共轴输出的耦合装置和方法
CN113252565B (zh) * 2021-05-31 2023-06-16 山东省日照市人民医院 一种基于ai人工智能的肺癌循环肿瘤细胞检测装置
CN115903216B (zh) * 2022-10-31 2025-08-22 南京大学 一种偏振选择的双波长消色差集成荧光成像器件及方法
KR20240142678A (ko) * 2023-03-22 2024-10-02 경북대학교 산학협력단 렌즈 위상 파면 변조 기능 및 파면 투과 기능을 제공하는 기하학적 위상 광학 소자

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH07234382A (ja) * 1994-02-24 1995-09-05 Matsushita Electric Ind Co Ltd 超解像走査光学装置
US5866911A (en) * 1994-07-15 1999-02-02 Baer; Stephen C. Method and apparatus for improving resolution in scanned optical system
JP3164989B2 (ja) 1994-12-28 2001-05-14 科学技術振興事業団 試料観察方法および多波長光光学顕微鏡
JPH1195120A (ja) * 1997-09-19 1999-04-09 Olympus Optical Co Ltd 顕微鏡の観察方法
JP3350442B2 (ja) * 1998-04-09 2002-11-25 科学技術振興事業団 顕微鏡システム
US6844963B2 (en) * 2000-03-23 2005-01-18 Olympus Optical Co., Ltd. Double-resonance-absorption microscope
JP4334835B2 (ja) * 2002-08-28 2009-09-30 独立行政法人科学技術振興機構 顕微鏡
JP3993553B2 (ja) * 2003-10-15 2007-10-17 独立行政法人科学技術振興機構 ３次元分析装置
JP2006047912A (ja) * 2004-08-09 2006-02-16 Olympus Corp 超解像顕微鏡
JP4258814B2 (ja) * 2004-11-11 2009-04-30 オリンパス株式会社 顕微鏡の照明装置
US7776613B2 (en) * 2006-08-07 2010-08-17 President And Fellows Of Harvard College Sub-diffraction image resolution and other imaging techniques
JP2008058003A (ja) * 2006-08-29 2008-03-13 Olympus Corp 顕微鏡
JP2010102332A (ja) * 2008-09-29 2010-05-06 Nikon Corp 光活性化限局顕微鏡及び光活性化限局観察方法

Also Published As

Publication number Publication date
CN102096180A (zh) 2011-06-15
US8536543B2 (en) 2013-09-17
JP2011123314A (ja) 2011-06-23
US20110140000A1 (en) 2011-06-16
CN102096180B (zh) 2015-10-28

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