JP5221516B2 - フィターゼ変異体 - Google Patents

フィターゼ変異体 Download PDF

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Publication number
JP5221516B2
JP5221516B2 JP2009503408A JP2009503408A JP5221516B2 JP 5221516 B2 JP5221516 B2 JP 5221516B2 JP 2009503408 A JP2009503408 A JP 2009503408A JP 2009503408 A JP2009503408 A JP 2009503408A JP 5221516 B2 JP5221516 B2 JP 5221516B2
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Japan
Prior art keywords
phytase
seq
activity
animal feed
protein
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Japanese (ja)
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JP2009532043A5 (enExample
JP2009532043A (ja
Inventor
マリア，レオナルド デ
アンデルセン，カルステン
コベレー スコウ，ラース
ブロム セレンセン，ミカエル
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ノボザイムス アクティーゼルスカブ
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Publication of JP2009532043A5 publication Critical patent/JP2009532043A5/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/03—Phosphoric monoester hydrolases (3.1.3)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Animal Husbandry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Fodder In General (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Detergent Compositions (AREA)
JP2009503408A 2006-04-04 2007-03-19 フィターゼ変異体 Active JP5221516B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
DKPA200600484 2006-04-04
DKPA200600484 2006-04-04
DKPA200600581 2006-04-25
DKPA200600581 2006-04-25
PCT/DK2007/000135 WO2007112739A1 (en) 2006-04-04 2007-03-19 Phytase variants

Publications (3)

Publication Number Publication Date
JP2009532043A JP2009532043A (ja) 2009-09-10
JP2009532043A5 JP2009532043A5 (enExample) 2013-01-24
JP5221516B2 true JP5221516B2 (ja) 2013-06-26

Family

ID=38123737

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2009503408A Active JP5221516B2 (ja) 2006-04-04 2007-03-19 フィターゼ変異体

Country Status (11)

Country Link
US (4) US8460656B2 (enExample)
EP (2) EP2365064B1 (enExample)
JP (1) JP5221516B2 (enExample)
AU (1) AU2007234177B2 (enExample)
BR (1) BRPI0709732B1 (enExample)
CA (1) CA2647476C (enExample)
DK (1) DK2365064T3 (enExample)
ES (2) ES2387203T3 (enExample)
MX (1) MX2008012632A (enExample)
PL (1) PL2365064T3 (enExample)
WO (1) WO2007112739A1 (enExample)

Families Citing this family (62)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6451572B1 (en) 1998-06-25 2002-09-17 Cornell Research Foundation, Inc. Overexpression of phytase genes in yeast systems
GB0422052D0 (en) 2004-10-04 2004-11-03 Dansico As Enzymes
DK2365064T3 (en) 2006-04-04 2015-03-30 Novozymes As Phytasevarianter
WO2008017066A2 (en) 2006-08-03 2008-02-07 Cornell Research Foundation, Inc. Phytases with improved thermal stability
EP2617823B1 (en) 2006-09-21 2015-07-01 BASF Enzymes LLC Phytases, nucleic acids encoding them and methods for making and using them
ES2426717T3 (es) 2008-09-26 2013-10-24 Novozymes A/S Variantes de fitasa de Hafnia
BRPI1008803A2 (pt) 2009-03-20 2015-08-25 Novozymes As Método para fabricar uma bebida não alcoólica
AU2010249500B2 (en) 2009-05-21 2016-03-24 Basf Enzymes Llc Phytases, nucleic acids encoding them and methods for making and using them
AU2013204082B2 (en) * 2009-05-21 2016-03-31 Basf Enzymes Llc Phytases, nucleic acids encoding them and methods for making and using them
DK2491120T3 (en) 2009-10-22 2016-03-21 Basf Se Synthetic phytasevarianter
RU2472855C2 (ru) * 2009-12-15 2013-01-20 Федеральное государственное унитарное предприятие "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов" (ФГУП ГосНИИгенетика) МУТАНТНАЯ РЕКОМБИНАНТНАЯ ТЕРМОСТАБИЛЬНАЯ ФИТАЗА (ВАРИАНТЫ), ФРАГМЕНТ ДНК, КОДИРУЮЩИЙ УКАЗАННУЮ ФИТАЗУ (ВАРИАНТЫ), ШТАММ Pichia pastoris - ПРОДУЦЕНТ УКАЗАННОЙ ФИТАЗЫ (ВАРИАНТЫ)
EP3101126B1 (en) * 2010-03-26 2019-10-09 Novozymes A/S Thermostable phytase variants
CA3105079A1 (en) * 2010-03-26 2011-09-29 Novozymes A/S Thermostable phytase variants
WO2011117406A1 (en) * 2010-03-26 2011-09-29 Novozymes A/S Thermostable phytase variants
CN103429093A (zh) * 2011-02-18 2013-12-04 杜邦营养生物科学有限公司 饲料添加剂组合物
GB201102865D0 (en) 2011-02-18 2011-04-06 Danisco Feed additive composition
GB201102857D0 (en) 2011-02-18 2011-04-06 Danisco Feed additive composition
ES2553458T3 (es) 2011-04-21 2015-12-09 Basf Se Variantes sintéticas de fitasa
DK2699674T3 (da) 2011-04-21 2016-03-21 Basf Se Syntetiske fytasevarianter
US20140322727A1 (en) 2011-07-07 2014-10-30 Dupont Nutrition Biosciences Aps Assay
GB201213801D0 (en) 2012-08-03 2012-09-12 Dupont Nutrition Biosci Aps Feed additive composition
CN105358694B (zh) 2013-03-08 2019-06-18 凯克应用生命科学研究生院 来自巴斯德毕赤酵母的酵母启动子
PT2964775T (pt) * 2013-03-08 2019-05-06 Biogrammatics Inc Promotores de leveduras para a expressão proteica
ES2800477T3 (es) 2013-09-11 2020-12-30 Novozymes As Procesos para producir productos de fermentación
AU2015279057B2 (en) 2014-06-27 2019-02-28 Dsm Ip Assets B.V. A method for improving the nutritional value of animal feed
MX385715B (es) * 2015-07-02 2025-03-18 Novozymes As Composiciones de pienso para animales y usos de las mismas.
WO2017112539A1 (en) 2015-12-22 2017-06-29 Novozymes A/S Process of extracting oil from thin stillage
TWI616532B (zh) * 2016-02-18 2018-03-01 基酵生物科技股份有限公司 提升耐熱性的植酸酶
TWI591180B (zh) 2016-02-18 2017-07-11 基酵生物科技股份有限公司 提升耐熱性的植酸酶
TWI607088B (zh) * 2016-02-18 2017-12-01 基酵生物科技股份有限公司 提升耐熱性的植酸酶
TWI615471B (zh) * 2016-02-18 2018-02-21 基酵生物科技股份有限公司 提升耐熱性的植酸酶
CN111164214A (zh) 2017-09-15 2020-05-15 诺维信公司 用于改进动物饲料营养质量的酶共混物和方法
BR112020007814A2 (pt) 2017-10-23 2020-10-20 Novozymes A/S processos para reduzir e/ou prevenir um aumento nos níveis de ácido lático em um sistema de fermentação de biocombustível e para produção de um produto de fermentação a partir de um material contendo amido, e, uso de um polipeptídeo, ou uma composição enzimática
EP3749107A1 (en) 2018-02-08 2020-12-16 Danisco US Inc. Thermally-resistant wax matrix particles for enzyme encapsulation
CN112367847A (zh) 2018-04-26 2021-02-12 丹尼斯科美国公司 增加固体组合物中植酸酶的稳定性的方法及包含磷酸盐和植酸酶的颗粒组合物
BR112020024347A2 (pt) 2018-05-31 2021-02-23 Novozymes A/S processos para aumentar o crescimento e a produtividade de levedura
RU2706086C1 (ru) * 2018-10-29 2019-11-13 Акционерное Общество "Биоамид" Мутантная рекомбинантная термостабильная фитаза
US12234464B2 (en) 2018-11-09 2025-02-25 Ginkgo Bioworks, Inc. Biosynthesis of mogrosides
US12365886B2 (en) 2018-11-20 2025-07-22 International N&H Denmark Aps Engineered robust high Tm-phytase clade polypeptides and fragments thereof
CN113302294A (zh) 2019-01-31 2021-08-24 诺维信公司 具有木聚糖酶活性的多肽及其用于改善动物饲料营养质量的用途
WO2021007379A1 (en) 2019-07-09 2021-01-14 Dupont Nutrition Biosciences Aps Fat coated particulate enzyme compositions
MX2022000831A (es) 2019-08-05 2022-02-10 Novozymes As Mezclas de enzimas y procesos para producir un ingrediente alimenticio de alto contenido proteico a partir de un subproducto de la vinaza entera.
US20220330576A1 (en) 2019-08-16 2022-10-20 Dupont Nutrition Biosciences Aps Compositions for gut health
WO2021046073A1 (en) 2019-09-05 2021-03-11 Dupont Nutrition Biosciences Aps Feed composition
BR112022007613A2 (pt) 2019-10-21 2022-08-23 Dupont Nutrition Biosci Aps Composição para a saúde do intestino
US20230023446A1 (en) 2019-12-16 2023-01-26 Novozymes A/S Processes for producing fermentation products
CN115867146B (zh) 2020-02-07 2025-01-17 国际N&H丹麦有限公司 用于动物健康的饲料组合物
CN115915956A (zh) 2020-02-28 2023-04-04 杜邦营养生物科学有限公司 饲料组合物
WO2022034211A1 (en) 2020-08-13 2022-02-17 Novozymes A/S Phytase variants and polynucleotides encoding same
CN117241678A (zh) 2020-10-16 2023-12-15 杜邦营养生物科学有限公司 用于动物健康的饲料组合物
WO2022169933A2 (en) 2021-02-03 2022-08-11 Dupont Nutrition Biosciences Aps Compositions for gut health
US20250120417A1 (en) 2021-09-27 2025-04-17 International N&H Denmark Aps Feed additive compositions and methods for using the same
WO2024137250A1 (en) 2022-12-19 2024-06-27 Novozymes A/S Carbohydrate esterase family 3 (ce3) polypeptides having acetyl xylan esterase activity and polynucleotides encoding same
EP4638768A2 (en) 2022-12-19 2025-10-29 Novozymes A/S Processes for producing fermentation products using fiber-degrading enzymes with engineered yeast
EP4638724A1 (en) 2022-12-19 2025-10-29 Novozymes A/S Carbohydrate esterase family 1 (ce1) polypeptides having ferulic acid esterase and/or acetyl xylan esterase activity and polynucleotides encoding same
WO2024137248A1 (en) 2022-12-19 2024-06-27 Novozymes A/S Compositions comprising arabinofuranosidases and a xylanase, and use thereof for increasing hemicellulosic fiber solubilization
WO2024137252A1 (en) 2022-12-19 2024-06-27 Novozymes A/S Process for reducing syrup viscosity in the backend of a process for producing a fermentation product
AU2024262752A1 (en) 2023-04-28 2025-10-16 International N&H Denmark Aps Ruminant feed additive compositions
WO2024258820A2 (en) 2023-06-13 2024-12-19 Novozymes A/S Processes for producing fermentation products using engineered yeast expressing a beta-xylosidase
WO2025059013A1 (en) 2023-09-11 2025-03-20 International N&H Denmark Aps Bacillus-based components for inhibiting or delaying the growth of enterococcus spp. in animals
WO2025128568A1 (en) 2023-12-11 2025-06-19 Novozymes A/S Composition and use thereof for increasing hemicellulosic fiber solubilization
WO2025217017A1 (en) 2024-04-08 2025-10-16 Novozymes A/S Compositions and methods for increasing phosphorous availability

Family Cites Families (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
NZ237549A (en) 1990-03-23 1993-06-25 Gist Brocades Nv Production of enhanced levels of enzymes in the seeds of transgenic plants and the use of these seeds
WO1992010755A1 (en) 1990-12-05 1992-06-25 Novo Nordisk A/S Proteins with changed epitopes and methods for the production thereof
DK91192D0 (da) 1992-07-10 1992-07-10 Novo Nordisk As Protein
JPH0795946B2 (ja) * 1993-08-19 1995-10-18 イチビキ株式会社 フィターゼ及びその製造法
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5689054A (en) 1994-03-17 1997-11-18 The United States Of America As Represented By The Secretary Of Agriculture Low phytic acid mutants and selection thereof
DE4422198C2 (de) 1994-06-24 1997-08-28 Audi Ag Verfahren zum Steuern der elektrischen Beheizung eines Katalysators
WO1996000787A1 (en) 1994-06-30 1996-01-11 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein
US5830732A (en) 1994-07-05 1998-11-03 Mitsui Toatsu Chemicals, Inc. Phytase
JP3570784B2 (ja) * 1994-07-05 2004-09-29 三井化学株式会社 新規なるフィターゼ
JPH10509324A (ja) 1994-11-24 1998-09-14 ノボ ノルディスク アクティーゼルスカブ 抑制されたアレルゲン性を有するポリペプチドの製造のための方法
AU697440B2 (en) 1994-12-07 1998-10-08 Novozymes A/S Polypeptide with reduced allergenicity
JP2002510963A (ja) 1997-01-10 2002-04-09 ノボザイムス アクティーゼルスカブ スキンケア用酵素修飾体
EP1017794A1 (en) 1997-02-06 2000-07-12 Novo Nordisk A/S Polypeptide-polymer conjugates having added and/or removed attachment groups
ES2296336T3 (es) 1997-06-25 2008-04-16 Novozymes A/S Polipeptido modificado.
NZ330940A (en) * 1997-07-24 2000-02-28 F Production of consensus phytases from fungal origin using computer programmes
US6720014B1 (en) 1997-08-13 2004-04-13 Diversa Corporation Phytase-containing foodstuffs and methods of making and using them
US5955310A (en) 1998-02-26 1999-09-21 Novo Nordisk Biotech, Inc. Methods for producing a polypeptide in a bacillus cell
AU765477B2 (en) * 1998-03-23 2003-09-18 Novozymes A/S Phytase variants
WO2000020569A1 (en) 1998-10-02 2000-04-13 Novozymes A/S Solid phytase compositions
WO2000022103A1 (en) 1998-10-13 2000-04-20 Novozymes A/S A modified polypeptide with reduced immune response
ATE390441T1 (de) 1998-10-30 2008-04-15 Novozymes As Niedrigallergene proteinvarianten
ATE365210T1 (de) 1998-10-30 2007-07-15 Novozymes As Glykosylierte proteine mit reduzierter allergenität
EP1170994A1 (en) 1999-04-23 2002-01-16 University Of Guelph Transgenic animals expressing salivary proteins
WO2001036607A1 (en) 1999-11-18 2001-05-25 Cornell Research Foundation, Inc. Site-directed mutagenesis of escherichia coli phytase
AU777210C (en) 2000-02-08 2005-08-04 Dsm Ip Assets B.V. Use of acid-stable subtilisin proteases in animal feed
AU2001254620A1 (en) 2000-04-28 2001-11-12 Novozymes A/S Lipolytic enzyme variant
US7319087B2 (en) 2001-05-04 2008-01-15 Novozymes A/S Antimicrobial polypeptide from Aspergillus niger
NZ532962A (en) 2001-11-20 2006-11-30 Novozymes As Antimicrobial polypeptides from pseudoplectania nigrella
WO2003048148A2 (en) 2001-12-03 2003-06-12 Novozymes A/S Statin-like compounds
CN1656217A (zh) 2002-05-30 2005-08-17 巴斯福股份公司 修饰的植酸酶
DE60335195D1 (de) 2002-08-12 2011-01-13 Danisco Us Inc Mutante e. coli-appa-phytaseenzyme und natürliche varianten davon, solche phytaseenzyme codierende nukleinsäuren, diese enthaltende vektoren und wirtszellen und verfahren zur herstellung und verwendung davon
WO2004085638A1 (en) * 2003-03-25 2004-10-07 Republic Of National Fisheries Research And Development Institute Phytase produced from citrobacter braakii
JP2005137293A (ja) * 2003-11-07 2005-06-02 Mitsui Chemicals Inc コドン改変と細胞外分泌の組合せにより外来フィターゼを植物で生産する方法
EP1809747B1 (en) 2004-10-04 2016-12-14 Novozymes A/S Polypeptides having phytase activity and polynucleotides encoding same
AR050895A1 (es) 2004-10-04 2006-11-29 Novozymes As Polipeptidos que tienen actividad de fitasa y polinucleotidos que los codifican
AU2005290934C1 (en) 2004-10-04 2013-05-09 Dupont Nutrition Biosciences Aps Citrobacter freundii phytase and homologues
GB0422052D0 (en) 2004-10-04 2004-11-03 Dansico As Enzymes
JP2006136314A (ja) * 2004-10-12 2006-06-01 Mitsui Chemicals Inc 新規改変フィターゼ
KR100663112B1 (ko) * 2004-10-29 2007-01-02 주식회사 기성기전 절전형 전자개폐기
WO2006063588A1 (en) 2004-12-13 2006-06-22 Novozymes A/S Polypeptides having acid phosphatase activity and polynucleotides encoding same
FR2888250B1 (fr) 2005-07-08 2007-08-17 Adisseo France Sas Soc Par Act Phytase de debaryomyces castellii
DK2365064T3 (en) 2006-04-04 2015-03-30 Novozymes As Phytasevarianter

Also Published As

Publication number Publication date
CA2647476A1 (en) 2007-10-11
PL2365064T3 (pl) 2015-05-29
US8460656B2 (en) 2013-06-11
US20130224332A1 (en) 2013-08-29
DK2365064T3 (en) 2015-03-30
BRPI0709732B1 (pt) 2017-06-06
MX2008012632A (es) 2008-10-13
CA2647476C (en) 2016-08-16
US20160362666A1 (en) 2016-12-15
US10041052B2 (en) 2018-08-07
US9451783B2 (en) 2016-09-27
EP2001999A1 (en) 2008-12-17
US20150024084A1 (en) 2015-01-22
AU2007234177A1 (en) 2007-10-11
EP2365064A1 (en) 2011-09-14
JP2009532043A (ja) 2009-09-10
BRPI0709732A2 (pt) 2011-07-26
US8877471B2 (en) 2014-11-04
ES2531434T3 (es) 2015-03-16
US20110258714A1 (en) 2011-10-20
EP2365064B1 (en) 2014-12-24
EP2001999B1 (en) 2012-05-16
AU2007234177B2 (en) 2012-11-08
WO2007112739A1 (en) 2007-10-11
ES2387203T3 (es) 2012-09-17

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