ES2531434T3 - Variantes de fitasa - Google Patents

Variantes de fitasa Download PDF

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Publication number
ES2531434T3
ES2531434T3 ES11155829.2T ES11155829T ES2531434T3 ES 2531434 T3 ES2531434 T3 ES 2531434T3 ES 11155829 T ES11155829 T ES 11155829T ES 2531434 T3 ES2531434 T3 ES 2531434T3
Authority
ES
Spain
Prior art keywords
activity
phytase
seq
incubated
determined
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES11155829.2T
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English (en)
Inventor
Leonardo De Maria
Carsten Andersen
Lars Kobberoee Skov
Mikael Blom Soerensen
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Novozymes AS
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Novozymes AS
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Publication date
Application filed by Novozymes AS filed Critical Novozymes AS
Application granted granted Critical
Publication of ES2531434T3 publication Critical patent/ES2531434T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/03—Phosphoric monoester hydrolases (3.1.3)

Abstract

Fitasa que tiene al menos 85% de identidad a la SEC ID NO:2, que tiene una termoestabilidad mejorada indicada como actividad residual determinada por división de un sobrenadante de fermentación en dos partes, una parte se incuba durante 30 minutos a 60°C, y la otra parte durante 30 minutos a 5°C, tras lo cual la actividad de ambas se determina en el fosfato de p-nitrofenilo a 37°C y pH 5.5, donde la actividad residual de la fitasa es la actividad de la muestra que ha sido incubada a 60°C dividido por la actividad de la misma muestra que ha sido incubada a 5°C, donde la actividad residual de la fitasa es al menos 120% de la actividad residual de la SEC ID NO: 2 de fitasa de referencia, medido en las mismas condiciones, y que comprende al menos una alteración en comparación con la SEC ID NO:2 en al menos una posición seleccionada de las siguientes: G52C/A99C, K141C/V199C, G59C/F100C, Q91C/W46C, N31C/E176C, N31C/T177C, y/o S162C/S247C, donde el grado de identidad entre secuencias, se determina por el programa "Needle" utilizando la matriz de sustitución BLOSUM62, la penalización de abertura de gap es 10, y la penalización de extensión de gap es 0.5.

Description

imagen1

Claims (1)

  1. imagen1
ES11155829.2T 2006-04-04 2007-03-19 Variantes de fitasa Active ES2531434T3 (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DK200600484 2006-04-04
DKPA200600484 2006-04-04
DKPA200600581 2006-04-25
DK200600581 2006-04-25

Publications (1)

Publication Number Publication Date
ES2531434T3 true ES2531434T3 (es) 2015-03-16

Family

ID=38123737

Family Applications (2)

Application Number Title Priority Date Filing Date
ES07711278T Active ES2387203T3 (es) 2006-04-04 2007-03-19 Variantes de fitasa
ES11155829.2T Active ES2531434T3 (es) 2006-04-04 2007-03-19 Variantes de fitasa

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES07711278T Active ES2387203T3 (es) 2006-04-04 2007-03-19 Variantes de fitasa

Country Status (11)

Country Link
US (4) US8460656B2 (es)
EP (2) EP2365064B1 (es)
JP (1) JP5221516B2 (es)
AU (1) AU2007234177B2 (es)
BR (1) BRPI0709732B1 (es)
CA (1) CA2647476C (es)
DK (1) DK2365064T3 (es)
ES (2) ES2387203T3 (es)
MX (1) MX2008012632A (es)
PL (1) PL2365064T3 (es)
WO (1) WO2007112739A1 (es)

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Also Published As

Publication number Publication date
BRPI0709732B1 (pt) 2017-06-06
US20110258714A1 (en) 2011-10-20
EP2001999A1 (en) 2008-12-17
WO2007112739A1 (en) 2007-10-11
EP2365064B1 (en) 2014-12-24
AU2007234177A1 (en) 2007-10-11
US20150024084A1 (en) 2015-01-22
JP5221516B2 (ja) 2013-06-26
AU2007234177B2 (en) 2012-11-08
MX2008012632A (es) 2008-10-13
US10041052B2 (en) 2018-08-07
CA2647476C (en) 2016-08-16
JP2009532043A (ja) 2009-09-10
US9451783B2 (en) 2016-09-27
US20160362666A1 (en) 2016-12-15
CA2647476A1 (en) 2007-10-11
ES2387203T3 (es) 2012-09-17
DK2365064T3 (en) 2015-03-30
US8877471B2 (en) 2014-11-04
BRPI0709732A2 (pt) 2011-07-26
PL2365064T3 (pl) 2015-05-29
EP2001999B1 (en) 2012-05-16
US8460656B2 (en) 2013-06-11
EP2365064A1 (en) 2011-09-14
US20130224332A1 (en) 2013-08-29

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