CA2647476C - Phytase variants - Google Patents

Phytase variants Download PDF

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Publication number
CA2647476C
CA2647476C CA2647476A CA2647476A CA2647476C CA 2647476 C CA2647476 C CA 2647476C CA 2647476 A CA2647476 A CA 2647476A CA 2647476 A CA2647476 A CA 2647476A CA 2647476 C CA2647476 C CA 2647476C
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CA
Canada
Prior art keywords
phytase
seq
variant
composition
activity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2647476A
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English (en)
French (fr)
Other versions
CA2647476A1 (en
Inventor
Leonardo De Maria
Carsten Andersen
Lars Kobberoee Skov
Mikael Blom Soerensen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
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Novozymes AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes AS filed Critical Novozymes AS
Publication of CA2647476A1 publication Critical patent/CA2647476A1/en
Application granted granted Critical
Publication of CA2647476C publication Critical patent/CA2647476C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/03—Phosphoric monoester hydrolases (3.1.3)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Animal Husbandry (AREA)
  • Food Science & Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Fodder In General (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Detergent Compositions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2647476A 2006-04-04 2007-03-19 Phytase variants Active CA2647476C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
DKPA200600484 2006-04-04
DKPA200600484 2006-04-04
DKPA200600581 2006-04-25
DKPA200600581 2006-04-25
PCT/DK2007/000135 WO2007112739A1 (en) 2006-04-04 2007-03-19 Phytase variants

Publications (2)

Publication Number Publication Date
CA2647476A1 CA2647476A1 (en) 2007-10-11
CA2647476C true CA2647476C (en) 2016-08-16

Family

ID=38123737

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2647476A Active CA2647476C (en) 2006-04-04 2007-03-19 Phytase variants

Country Status (11)

Country Link
US (4) US8460656B2 (enExample)
EP (2) EP2001999B1 (enExample)
JP (1) JP5221516B2 (enExample)
AU (1) AU2007234177B2 (enExample)
BR (1) BRPI0709732B1 (enExample)
CA (1) CA2647476C (enExample)
DK (1) DK2365064T3 (enExample)
ES (2) ES2531434T3 (enExample)
MX (1) MX2008012632A (enExample)
PL (1) PL2365064T3 (enExample)
WO (1) WO2007112739A1 (enExample)

Families Citing this family (64)

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RU2472855C2 (ru) * 2009-12-15 2013-01-20 Федеральное государственное унитарное предприятие "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов" (ФГУП ГосНИИгенетика) МУТАНТНАЯ РЕКОМБИНАНТНАЯ ТЕРМОСТАБИЛЬНАЯ ФИТАЗА (ВАРИАНТЫ), ФРАГМЕНТ ДНК, КОДИРУЮЩИЙ УКАЗАННУЮ ФИТАЗУ (ВАРИАНТЫ), ШТАММ Pichia pastoris - ПРОДУЦЕНТ УКАЗАННОЙ ФИТАЗЫ (ВАРИАНТЫ)
US20130017185A1 (en) * 2010-03-26 2013-01-17 Novozymes A/S Thermostable Phytase Variants
CN102917600B (zh) * 2010-03-26 2015-07-08 诺维信公司 热稳定性肌醇六磷酸酶变体
ES2723050T3 (es) * 2010-03-26 2019-08-21 Novozymes As Variantes de fitasa termoestables
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GB201213801D0 (en) 2012-08-03 2012-09-12 Dupont Nutrition Biosci Aps Feed additive composition
BR112015021753B1 (pt) 2013-03-08 2023-02-23 Keck Graduate Institute Of Applied Life Sciences Ácidos nucleicos isolados de pichia pastoris, vetor de expressão, células hospedeiras de levedura e construto de dna, bem como método para produzir uma proteína
DK2964775T3 (en) * 2013-03-08 2019-04-15 Biogrammatics Inc Yeast promoters for protein expression
US9951364B2 (en) 2013-09-11 2018-04-24 Novozymes A/S Processes for producing fermentation products
AU2015279057B2 (en) 2014-06-27 2019-02-28 Novonesis Animal Biosolutions Ag A method for improving the nutritional value of animal feed
WO2017001701A1 (en) * 2015-07-02 2017-01-05 Novozymes A/S Animal feed compositions and uses thereof
CN108699572A (zh) 2015-12-22 2018-10-23 诺维信公司 使用磷脂酶c增加发酵产物得率的方法
TWI615471B (zh) * 2016-02-18 2018-02-21 基酵生物科技股份有限公司 提升耐熱性的植酸酶
TWI616532B (zh) * 2016-02-18 2018-03-01 基酵生物科技股份有限公司 提升耐熱性的植酸酶
TWI607088B (zh) * 2016-02-18 2017-12-01 基酵生物科技股份有限公司 提升耐熱性的植酸酶
TWI591180B (zh) 2016-02-18 2017-07-11 基酵生物科技股份有限公司 提升耐熱性的植酸酶
CN111164214A (zh) 2017-09-15 2020-05-15 诺维信公司 用于改进动物饲料营养质量的酶共混物和方法
US11326187B2 (en) 2017-10-23 2022-05-10 Novozymes A/S Processes for producing a fermentation product
BR112020016068A2 (pt) 2018-02-08 2020-12-08 Danisco Us Inc. Partículas cera termicamente resistente matriz para encapsulamento de enzima
EP3784049A1 (en) 2018-04-26 2021-03-03 Danisco US Inc. Method for increasing stability of phytase in a solid composition and a granule composition comprising phosphate and phytase
WO2019231944A2 (en) 2018-05-31 2019-12-05 Novozymes A/S Processes for enhancing yeast growth and productivity
RU2706086C1 (ru) * 2018-10-29 2019-11-13 Акционерное Общество "Биоамид" Мутантная рекомбинантная термостабильная фитаза
CA3118924A1 (en) 2018-11-09 2020-05-14 Ginkgo Bioworks, Inc. Biosynthesis of mogrosides
KR20210094587A (ko) 2018-11-20 2021-07-29 듀폰 뉴트리션 바이오사이언시즈 에이피에스 조작된 견고한 고 Tm-피타제 클레이드 폴리펩타이드 및 이의 단편
WO2020160126A1 (en) 2019-01-31 2020-08-06 Novozymes A/S Polypeptides having xylanase activity and use thereof for improving the nutritional quality of animal feed
BR112022000351A2 (pt) 2019-07-09 2022-05-10 Dupont Nutrition Biosci Aps Composições de enzima particuladas revestidas de gordura
EP4009807A1 (en) 2019-08-05 2022-06-15 Novozymes A/S Enzyme blends and processes for producing a high protein feed ingredient from a whole stillage byproduct
US12610970B2 (en) 2019-08-16 2026-04-28 International N&H Denmark Aps Compositions for gut health
WO2021046073A1 (en) 2019-09-05 2021-03-11 Dupont Nutrition Biosciences Aps Feed composition
EP4048081A2 (en) 2019-10-21 2022-08-31 DuPont Nutrition Biosciences ApS Compositions for gut health
MX2022006438A (es) 2019-12-16 2022-06-22 Novozymes As Procesos para obtener productos de fermentacion.
AU2021217391B2 (en) 2020-02-07 2026-03-26 International N&H Denmark Aps Feed compositions for animal health
US20230240334A1 (en) 2020-02-28 2023-08-03 Dupont Nutrition Biosciences Aps Feed compositions
JP2023537982A (ja) 2020-08-13 2023-09-06 ノボザイムス アクティーゼルスカブ フィターゼ変異体及びそれをコードするポリヌクレオチド
CN117241678A (zh) 2020-10-16 2023-12-15 杜邦营养生物科学有限公司 用于动物健康的饲料组合物
WO2022169933A2 (en) 2021-02-03 2022-08-11 Dupont Nutrition Biosciences Aps Compositions for gut health
EP4408186A1 (en) 2021-09-27 2024-08-07 International N&H Denmark ApS Feed additive compositions and methods for using the same
WO2024137248A1 (en) 2022-12-19 2024-06-27 Novozymes A/S Compositions comprising arabinofuranosidases and a xylanase, and use thereof for increasing hemicellulosic fiber solubilization
EP4638767A1 (en) 2022-12-19 2025-10-29 Novozymes A/S Process for reducing syrup viscosity in the backend of a process for producing a fermentation product
CN120283060A (zh) 2022-12-19 2025-07-08 诺维信公司 用于使用纤维降解酶和工程化酵母生产发酵产物的工艺
CN120380140A (zh) 2022-12-19 2025-07-25 诺维信公司 具有阿魏酸酯酶和/或乙酰木聚糖酯酶活性的碳水化合物酯酶家族1(ce1)多肽和编码其的多核苷酸
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AU2024262752A1 (en) 2023-04-28 2025-10-16 International N&H Denmark Aps Ruminant feed additive compositions
AU2024303961A1 (en) 2023-06-13 2025-12-11 Novozymes A/S Processes for producing fermentation products using engineered yeast expressing a beta-xylosidase
CN122161503A (zh) 2023-09-11 2026-06-05 国际营养与健康丹麦有限公司 用于抑制或延缓动物体内肠球菌属物种的生长的基于芽孢杆菌的组分
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Also Published As

Publication number Publication date
WO2007112739A1 (en) 2007-10-11
CA2647476A1 (en) 2007-10-11
US20150024084A1 (en) 2015-01-22
ES2387203T3 (es) 2012-09-17
US10041052B2 (en) 2018-08-07
AU2007234177A1 (en) 2007-10-11
EP2365064A1 (en) 2011-09-14
EP2365064B1 (en) 2014-12-24
US8877471B2 (en) 2014-11-04
EP2001999B1 (en) 2012-05-16
US20130224332A1 (en) 2013-08-29
US20110258714A1 (en) 2011-10-20
BRPI0709732A2 (pt) 2011-07-26
MX2008012632A (es) 2008-10-13
BRPI0709732B1 (pt) 2017-06-06
ES2531434T3 (es) 2015-03-16
DK2365064T3 (en) 2015-03-30
EP2001999A1 (en) 2008-12-17
JP2009532043A (ja) 2009-09-10
JP5221516B2 (ja) 2013-06-26
US8460656B2 (en) 2013-06-11
US20160362666A1 (en) 2016-12-15
US9451783B2 (en) 2016-09-27
PL2365064T3 (pl) 2015-05-29
AU2007234177B2 (en) 2012-11-08

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