JP3961570B2 - 植物プロトポルフィリノーゲンオキシダーゼをコードするdna分子およびその阻害物質耐性突然変異体 - Google Patents
植物プロトポルフィリノーゲンオキシダーゼをコードするdna分子およびその阻害物質耐性突然変異体 Download PDFInfo
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8214—Plastid transformation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
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- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Applications Claiming Priority (7)
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| US1361296P | 1996-02-28 | 1996-02-28 | |
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| US2000396P | 1996-06-21 | 1996-06-21 | |
| US60/020,003 | 1996-06-21 | ||
| PCT/US1997/003313 WO1997032011A1 (en) | 1996-02-28 | 1997-02-27 | Dna molecules encoding plant protoporphyrinogen oxidase and inhibitor-resistant mutants thereof |
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| JP9531213A Ceased JP2000506011A (ja) | 1996-02-28 | 1997-02-27 | 植物のプロトポルフィリノーゲンオキシダーゼ遺伝子由来のプロモーター |
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| JP9531213A Ceased JP2000506011A (ja) | 1996-02-28 | 1997-02-27 | 植物のプロトポルフィリノーゲンオキシダーゼ遺伝子由来のプロモーター |
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| US (1) | US6018105A (enExample) |
| EP (2) | EP0885305A1 (enExample) |
| JP (2) | JP3961570B2 (enExample) |
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| US5767373A (en) | 1994-06-16 | 1998-06-16 | Novartis Finance Corporation | Manipulation of protoporphyrinogen oxidase enzyme activity in eukaryotic organisms |
| US6084155A (en) | 1995-06-06 | 2000-07-04 | Novartis Ag | Herbicide-tolerant protoporphyrinogen oxidase ("protox") genes |
| WO1997004088A1 (en) | 1995-07-20 | 1997-02-06 | Sumitomo Chemical Company, Ltd. | Porphyrin-accumulating type herbicide resistance gene |
| WO1998029554A1 (en) * | 1996-12-27 | 1998-07-09 | Sumitomo Chemical Co., Ltd. | Methods of conferring ppo-inhibiting herbicide resistance to plants by gene manipulation |
| ZA98371B (en) * | 1997-01-31 | 1999-07-16 | Du Pont | Genetically transformed plants demonstrating resistance to porphyrinogen biosynthesis-inhibiting herbicides. |
| EP1020525A4 (en) * | 1997-09-11 | 2004-08-25 | Nihon Nohyaku Co Ltd | PROTOPORPHYRINOGEN OXIDASE, WHICH IS TOLERANT TO HERBICIDES NEEDING LIGHT |
| US6362398B1 (en) | 1998-03-11 | 2002-03-26 | Syngenta Participations Ag | ClpP plastid promoter sequence |
| BR9908624A (pt) * | 1998-03-11 | 2000-11-14 | Novartis Ag | Sequência de promotor de plastìdio de planta |
| AU769868B2 (en) | 1998-04-10 | 2004-02-05 | Sumitomo Chemical Company, Limited | A method for evaluating the ability of a compound to inhibit the protoporphyrinogen oxidase activity |
| JP4788011B2 (ja) * | 1998-04-30 | 2011-10-05 | 住友化学株式会社 | 雑草防除剤耐性の付与方法 |
| AU753020B2 (en) | 1998-04-30 | 2002-10-03 | Sumitomo Chemical Company, Limited | Method for giving resistance to weed control compounds to plants |
| US6906245B1 (en) | 1998-04-30 | 2005-06-14 | Sumitomo Chemical Company, Limited | Method for producing transgenic plants resistant to weed control compounds which disrupt the porphyrin pathways of plants |
| AR020078A1 (es) | 1998-05-26 | 2002-04-10 | Syngenta Participations Ag | Metodo para alterar la expresion de un gen objetivo en una celula de planta |
| ES2281183T3 (es) * | 1998-07-10 | 2007-09-16 | Calgene Llc | Expresion de genes de tolerancia a herbicida en plastidios vegetales. |
| US6492578B1 (en) | 1998-07-10 | 2002-12-10 | Calgene Llc | Expression of herbicide tolerance genes in plant plastids |
| PL347628A1 (en) * | 1998-11-10 | 2002-04-22 | Syngenta Participations Ag | Herbicidal composition |
| RU2240001C2 (ru) * | 1998-11-10 | 2004-11-20 | Зингента Партисипейшнс Аг | Гербицидная композиция и способ борьбы с нежелательной растительностью в посевах культурных растений с использованием этой композиции |
| GB9828201D0 (en) * | 1998-12-21 | 1999-02-17 | Zenco No 4 Ltd | Genetic modification of compositae |
| WO2001007590A2 (en) * | 1999-07-27 | 2001-02-01 | Syngenta Participations Ag | Chimeric genes for plastid expression |
| CA2381927A1 (en) * | 1999-08-13 | 2001-02-22 | Syngenta Participations Ag | Herbicide-tolerant protoporphyrinogen oxidase |
| US6617498B1 (en) * | 1999-09-03 | 2003-09-09 | Pioneer-Hi-Bred International, Inc. | Inducible promoters |
| BR0014681A (pt) * | 1999-10-11 | 2002-08-20 | Processo para aumentar o rendimento ou a biomassa da colheita usando o gene para protoporfirinógeno oxidase | |
| JP4821036B2 (ja) * | 1999-10-29 | 2011-11-24 | 住友化学株式会社 | 除草剤耐性植物 |
| WO2001036606A2 (de) * | 1999-11-16 | 2001-05-25 | Basf Plant Science Gmbh | Protoporphyrinogen-ix-oxidase und seine verwendung |
| WO2001044457A2 (en) | 1999-12-16 | 2001-06-21 | Monsanto Technology Llc | Dna constructs for expression of heterologous polypeptides in plants |
| WO2001068826A2 (en) * | 2000-03-14 | 2001-09-20 | Syngenta Participations Ag | Protoporphyrinogen oxidase ('protox') genes |
| US6713259B2 (en) * | 2000-09-13 | 2004-03-30 | Monsanto Technology Llc | Corn event MON810 and compositions and methods for detection thereof |
| AR037413A1 (es) * | 2001-11-27 | 2004-11-10 | Valent Biosciences Corp | Composicion herbicida intensificada |
| CN101018864A (zh) | 2002-12-26 | 2007-08-15 | 先正达合作有限公司 | 细胞增殖相关多肽及其应用 |
| ATE495264T1 (de) | 2003-10-06 | 2011-01-15 | Syngenta Participations Ag | In pflanzenplastiden funktionsfähiger promotor |
| EP1723245A4 (en) | 2004-03-08 | 2010-03-03 | Syngenta Participations Ag | GLUTINAUTIC MAIZE PROTEIN AND PROMOTER |
| JP4720223B2 (ja) | 2004-05-18 | 2011-07-13 | 住友化学株式会社 | 除草活性化合物耐性植物 |
| CA2584934A1 (en) | 2007-04-17 | 2008-10-17 | University Of Guelph | Nitrogen-regulated sugar sensing gene and protein and modulation thereof |
| US9347046B2 (en) | 2009-01-22 | 2016-05-24 | Syngenta Participations Ag | Hydroxyphenylpyruvate dioxygenase polypeptides and methods of use |
| AU2010206619A1 (en) | 2009-01-22 | 2011-07-28 | Syngenta Participations Ag | Mutant hydroxyphenylpyruvate dioxygenase polypeptides and methods of use |
| AU2010210645A1 (en) | 2009-02-06 | 2011-07-07 | Syngenta Participations Ag | Modification of multidomain enzyme for expression in plants |
| UA112969C2 (uk) * | 2010-08-03 | 2016-11-25 | Сібас Юс Ллс | Рослина, стійка до одного або більше ррх-інгібуючих гербіцидів, яка містить мутантний ген протопорфіриноген ix оксидази (ррх) |
| JP2012056817A (ja) * | 2010-09-10 | 2012-03-22 | Kochi Univ Of Technology | 単細胞藻類の細胞破砕液を利用したアミノ酸含有有機液肥 |
| MX347362B (es) * | 2010-12-16 | 2017-04-10 | Basf Agro Bv | Plantas que tienen mayor tolerancia a herbicidas. |
| AR091489A1 (es) | 2012-06-19 | 2015-02-11 | Basf Se | Plantas que tienen una mayor tolerancia a herbicidas inhibidores de la protoporfirinogeno oxidasa (ppo) |
| US10041087B2 (en) | 2012-06-19 | 2018-08-07 | BASF Agro B.V. | Plants having increased tolerance to herbicides |
| CN104107437B (zh) * | 2013-06-09 | 2015-08-26 | 厦门成坤生物技术有限公司 | 一种用于治疗乙型病毒性肝炎的rna干扰组合物及其制备方法 |
| BR112016002835A2 (pt) * | 2013-08-12 | 2017-09-19 | Basf Agro Bv | Plantas, semente, células vegetais, produtos vegetais, prole ou planta descendente derivada de uma planta, método de controle de ervas, método de produção de planta, método de produção de plantas de prole, método de produção de produtos vegetais, método de cultivo da planta, combinação útil para controle de ervas, processos de preparação da combinação útil para controle de ervas e uso da combinação |
| EA201690374A1 (ru) * | 2013-08-12 | 2016-07-29 | Басф Агро Б.В. | Растения с повышенной устойчивостью к гербицидам |
| UA127470C2 (uk) * | 2015-06-17 | 2023-09-06 | Басф Агро Б.В. | Рослина, яка має підвищену толерантність до гербіцидів |
| CN111423990B (zh) * | 2020-04-10 | 2021-08-27 | 科稷达隆(北京)生物技术有限公司 | 一种乙氧氟草醚敏感型酵母菌及其制备方法 |
| MX2023006053A (es) | 2020-11-24 | 2023-06-06 | Syngenta Crop Protection Ag | Compuestos herbicidas. |
| CN118956784A (zh) * | 2021-04-02 | 2024-11-15 | 青岛清原种子科学有限公司 | 对ppo抑制剂类除草剂具有耐受性的ppo多肽及应用 |
| CN115247157A (zh) * | 2021-04-02 | 2022-10-28 | 青岛清原化合物有限公司 | 对ppo抑制剂类除草剂具有耐受性的ppo多肽及应用 |
| EP4320122A1 (en) | 2021-04-07 | 2024-02-14 | Syngenta Crop Protection AG | Herbicidal compounds |
| CN115340987B (zh) * | 2021-05-12 | 2023-12-01 | 北京大北农生物技术有限公司 | 除草剂耐受性蛋白质、其编码基因及用途 |
| CA3244844A1 (en) | 2022-03-11 | 2023-09-14 | Syngenta Crop Protection Ag | HERBICIDE COMPOUNDS |
| AU2023243482A1 (en) * | 2022-03-29 | 2024-09-12 | Qingdao Kingagroot Chemical Compound Co., Ltd. | Ppo2 polypeptide having tolerance to ppo inhibitor herbicide and application |
| IL292199B2 (en) | 2022-04-12 | 2024-02-01 | Plantarc Bio Ltd | A method for optimizing gene expression levels in plants |
| PY2334474A (es) | 2022-05-20 | 2023-11-21 | Syngenta Crop Protection Ag | Compuestos herbicidas |
| PY2352054A (es) | 2022-07-13 | 2024-01-30 | Syngenta Crop Protection Ag | Compuestos herbicidas |
| WO2024175476A1 (en) | 2023-02-24 | 2024-08-29 | Syngenta Crop Protection Ag | Herbicidal compositions |
| EP4669106A1 (en) | 2023-02-24 | 2025-12-31 | Syngenta Crop Protection AG | HERBICIDE COMPOSITIONS |
| WO2024194063A1 (en) | 2023-03-17 | 2024-09-26 | Syngenta Crop Protection Ag | Herbicidal triazine derivatives |
| CN121443146A (zh) | 2023-07-20 | 2026-01-30 | 先正达农作物保护股份公司 | 除草组合物 |
| AU2024292019A1 (en) | 2023-07-20 | 2026-01-15 | Syngenta Crop Protection Ag | Herbicidal compositions |
| CN120230766B (zh) * | 2025-05-29 | 2025-08-26 | 隆平生物技术(海南)有限公司 | 耐除草剂基因ppo的变体及其编码蛋白和应用 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0360750A3 (en) * | 1988-09-22 | 1991-01-02 | Ciba-Geigy Ag | Novel herbicide tolerant plants |
| CN1039283C (zh) * | 1988-12-12 | 1998-07-29 | Fmc公司 | 卟啉的应用 |
| NZ231658A (en) * | 1988-12-12 | 1992-05-26 | Fmc Corp | Inhibitors of protoporphyrinogen oxidase and compositions for killing tumour cells |
| US5086169A (en) * | 1989-04-20 | 1992-02-04 | The Research Foundation Of State University Of New York | Isolated pollen-specific promoter of corn |
| US5451513A (en) * | 1990-05-01 | 1995-09-19 | The State University of New Jersey Rutgers | Method for stably transforming plastids of multicellular plants |
| ATE195554T1 (de) * | 1990-05-18 | 2000-09-15 | Mycogen Plant Science Inc | Rekombinanter promoter zur genexpression in monokotylen |
| IL98405A0 (en) * | 1990-06-11 | 1992-07-15 | Fmc Corp | Pharmaceutical compositions containing enzyme inhibiting agents |
| US5290926A (en) * | 1990-09-14 | 1994-03-01 | Ciba-Geigy Corporation | Isolated DNA Encoding plant histidinol dehydrogenase |
| CA2193072A1 (en) * | 1994-06-14 | 1995-12-21 | Derek Chalmers | Corticotropin-releasing factor2 receptors |
| US5767373A (en) * | 1994-06-16 | 1998-06-16 | Novartis Finance Corporation | Manipulation of protoporphyrinogen oxidase enzyme activity in eukaryotic organisms |
| WO1997004088A1 (en) * | 1995-07-20 | 1997-02-06 | Sumitomo Chemical Company, Ltd. | Porphyrin-accumulating type herbicide resistance gene |
| ATE266093T1 (de) * | 1995-08-10 | 2004-05-15 | Univ Rutgers | Zellkern-codiertes transkriptionssystem plastiden höherer pflanzen |
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