JP2021513330A - ヒトインスリンアナログのコドン最適化前駆体遺伝子およびシグナルペプチド遺伝子 - Google Patents

ヒトインスリンアナログのコドン最適化前駆体遺伝子およびシグナルペプチド遺伝子 Download PDF

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JP2021513330A
JP2021513330A JP2020541883A JP2020541883A JP2021513330A JP 2021513330 A JP2021513330 A JP 2021513330A JP 2020541883 A JP2020541883 A JP 2020541883A JP 2020541883 A JP2020541883 A JP 2020541883A JP 2021513330 A JP2021513330 A JP 2021513330A
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nucleic acid
acid molecule
seq
human insulin
molecule encoding
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JP2021513330A5 (https=
Inventor
菲菲 王
菲菲 王
磊 陳
磊 陳
宏偉 王
宏偉 王
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Jiangsu Hengrui Pharmaceutical Co Ltd
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Jiangsu Hengrui Pharmaceutical Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/84—Pichia

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
JP2020541883A 2018-02-09 2019-02-01 ヒトインスリンアナログのコドン最適化前駆体遺伝子およびシグナルペプチド遺伝子 Withdrawn JP2021513330A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN201810131794 2018-02-09
CN201810131794.3 2018-02-09
PCT/CN2019/074384 WO2019154311A1 (zh) 2018-02-09 2019-02-01 一种密码子优化的人胰岛素类似物前体基因和信号肽基因

Publications (2)

Publication Number Publication Date
JP2021513330A true JP2021513330A (ja) 2021-05-27
JP2021513330A5 JP2021513330A5 (https=) 2022-02-07

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JP2020541883A Withdrawn JP2021513330A (ja) 2018-02-09 2019-02-01 ヒトインスリンアナログのコドン最適化前駆体遺伝子およびシグナルペプチド遺伝子

Country Status (12)

Country Link
US (1) US20210032307A1 (https=)
EP (1) EP3750998A4 (https=)
JP (1) JP2021513330A (https=)
KR (1) KR20200119237A (https=)
CN (1) CN111094572B (https=)
AU (1) AU2019218315A1 (https=)
BR (1) BR112020015238A2 (https=)
CA (1) CA3086618A1 (https=)
MX (1) MX2020007929A (https=)
RU (1) RU2020128190A (https=)
TW (1) TW201934752A (https=)
WO (1) WO2019154311A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111560379B (zh) * 2020-05-26 2022-09-13 北京惠之衡生物科技有限公司 密码子优化的门冬胰岛素前体基因、重组载体、基因工程菌及其应用
CN116751817B (zh) * 2023-05-29 2024-07-09 广东东阳光药业股份有限公司 检测胰岛素或其类似物的生物学活性的方法及试剂盒
CN117384275B (zh) * 2023-12-13 2024-03-15 北京科为博生物科技有限公司 胰岛素样生长因子突变体igf1m及其应用

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1340772C (en) * 1987-12-30 1999-09-28 Patricia Tekamp-Olson Expression and secretion of heterologous protiens in yeast employing truncated alpha-factor leader sequences
EP1132404A3 (en) 1993-09-17 2002-03-27 Novo Nordisk A/S Acylated insulin
US5639642A (en) * 1994-06-16 1997-06-17 Novo Nordisk A/S Synthetic leader peptide sequences
ES2258797T3 (es) * 1996-12-20 2006-09-01 Novo Nordisk A/S Proteinas con extension n-terminal expresadas en la levadura.
CN1302333A (zh) * 1998-01-23 2001-07-04 诺沃挪第克公司 在酵母中制备目的多肽的方法
AR025646A1 (es) * 2000-09-13 2002-12-04 Beta Lab Sa Cepa de levaduras metilotroficas recombinantes productoras de un precursor de insulina, construcciones de adn y metodo para obtener la cepa.
PL1633876T3 (pl) * 2003-06-17 2009-01-30 Sembiosys Genetics Inc Sposoby wytwarzania insuliny w roślinach
CA2531988C (en) 2003-08-05 2016-06-28 Novo Nordisk A/S Novel insulin derivatives
CN1873006A (zh) * 2005-05-30 2006-12-06 上海新生源医药研究有限公司 一种重组人胰岛素原的生产方法
MY161552A (en) * 2009-03-12 2017-04-28 Bigtec Private Ltd A polynucleotide and polypeptide sequence and methods thereof
AR087433A1 (es) * 2011-08-08 2014-03-26 Merck Sharp & Dohme Analogos de insulina n-glicosilados
KR20140104994A (ko) * 2011-12-15 2014-08-29 샹하이 헨그루이 파마수티컬 컴퍼니 리미티드 인간 인슐린 유사체 및 이의 아실화 유도체
WO2018024186A1 (zh) 2016-08-02 2018-02-08 江苏恒瑞医药股份有限公司 一种人胰岛素或其类似物的酰化衍生物

Also Published As

Publication number Publication date
EP3750998A4 (en) 2022-02-23
RU2020128190A3 (https=) 2022-03-09
MX2020007929A (es) 2020-10-01
BR112020015238A2 (pt) 2020-12-29
CA3086618A1 (en) 2019-08-15
KR20200119237A (ko) 2020-10-19
TW201934752A (zh) 2019-09-01
AU2019218315A1 (en) 2020-07-09
CN111094572B (zh) 2023-04-04
US20210032307A1 (en) 2021-02-04
CN111094572A (zh) 2020-05-01
WO2019154311A1 (zh) 2019-08-15
RU2020128190A (ru) 2022-03-09
EP3750998A1 (en) 2020-12-16

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