CN111094572B - 一种密码子优化的人胰岛素类似物前体基因和信号肽基因 - Google Patents

一种密码子优化的人胰岛素类似物前体基因和信号肽基因 Download PDF

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CN111094572B
CN111094572B CN201980004477.8A CN201980004477A CN111094572B CN 111094572 B CN111094572 B CN 111094572B CN 201980004477 A CN201980004477 A CN 201980004477A CN 111094572 B CN111094572 B CN 111094572B
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nucleic acid
acid molecule
human insulin
molecule encoding
precursor
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CN111094572A (zh
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王菲菲
陈磊
王宏伟
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Jiangsu Hengrui Medicine Co Ltd
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Jiangsu Hengrui Medicine Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/84—Pichia

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
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  • General Engineering & Computer Science (AREA)
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  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
CN201980004477.8A 2018-02-09 2019-02-01 一种密码子优化的人胰岛素类似物前体基因和信号肽基因 Active CN111094572B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN201810131794 2018-02-09
CN2018101317943 2018-02-09
PCT/CN2019/074384 WO2019154311A1 (zh) 2018-02-09 2019-02-01 一种密码子优化的人胰岛素类似物前体基因和信号肽基因

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CN111094572A CN111094572A (zh) 2020-05-01
CN111094572B true CN111094572B (zh) 2023-04-04

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US (1) US20210032307A1 (https=)
EP (1) EP3750998A4 (https=)
JP (1) JP2021513330A (https=)
KR (1) KR20200119237A (https=)
CN (1) CN111094572B (https=)
AU (1) AU2019218315A1 (https=)
BR (1) BR112020015238A2 (https=)
CA (1) CA3086618A1 (https=)
MX (1) MX2020007929A (https=)
RU (1) RU2020128190A (https=)
TW (1) TW201934752A (https=)
WO (1) WO2019154311A1 (https=)

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Publication number Priority date Publication date Assignee Title
CN111560379B (zh) * 2020-05-26 2022-09-13 北京惠之衡生物科技有限公司 密码子优化的门冬胰岛素前体基因、重组载体、基因工程菌及其应用
CN116751817B (zh) * 2023-05-29 2024-07-09 广东东阳光药业股份有限公司 检测胰岛素或其类似物的生物学活性的方法及试剂盒
CN117384275B (zh) * 2023-12-13 2024-03-15 北京科为博生物科技有限公司 胰岛素样生长因子突变体igf1m及其应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998028429A1 (en) * 1996-12-20 1998-07-02 Novo Nordisk A/S N-terminally extended proteins expressed in yeast
CN1873006A (zh) * 2005-05-30 2006-12-06 上海新生源医药研究有限公司 一种重组人胰岛素原的生产方法
CN102439154A (zh) * 2009-03-12 2012-05-02 比格科技私人有限公司 一种多核苷酸和多肽链序列及其方法
CN103889444A (zh) * 2011-08-08 2014-06-25 默沙东公司 N-糖基化的胰岛素类似物

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CA1340772C (en) * 1987-12-30 1999-09-28 Patricia Tekamp-Olson Expression and secretion of heterologous protiens in yeast employing truncated alpha-factor leader sequences
EP1132404A3 (en) 1993-09-17 2002-03-27 Novo Nordisk A/S Acylated insulin
US5639642A (en) * 1994-06-16 1997-06-17 Novo Nordisk A/S Synthetic leader peptide sequences
CN1302333A (zh) * 1998-01-23 2001-07-04 诺沃挪第克公司 在酵母中制备目的多肽的方法
AR025646A1 (es) * 2000-09-13 2002-12-04 Beta Lab Sa Cepa de levaduras metilotroficas recombinantes productoras de un precursor de insulina, construcciones de adn y metodo para obtener la cepa.
PL1633876T3 (pl) * 2003-06-17 2009-01-30 Sembiosys Genetics Inc Sposoby wytwarzania insuliny w roślinach
CA2531988C (en) 2003-08-05 2016-06-28 Novo Nordisk A/S Novel insulin derivatives
KR20140104994A (ko) * 2011-12-15 2014-08-29 샹하이 헨그루이 파마수티컬 컴퍼니 리미티드 인간 인슐린 유사체 및 이의 아실화 유도체
WO2018024186A1 (zh) 2016-08-02 2018-02-08 江苏恒瑞医药股份有限公司 一种人胰岛素或其类似物的酰化衍生物

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998028429A1 (en) * 1996-12-20 1998-07-02 Novo Nordisk A/S N-terminally extended proteins expressed in yeast
CN1873006A (zh) * 2005-05-30 2006-12-06 上海新生源医药研究有限公司 一种重组人胰岛素原的生产方法
CN102439154A (zh) * 2009-03-12 2012-05-02 比格科技私人有限公司 一种多核苷酸和多肽链序列及其方法
CN103889444A (zh) * 2011-08-08 2014-06-25 默沙东公司 N-糖基化的胰岛素类似物

Non-Patent Citations (1)

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Title
陈频等.全科医师糖尿病临床诊疗手册.《全科医师糖尿病临床诊疗手册》.福建科学技术出版社,2017,第114页. *

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JP2021513330A (ja) 2021-05-27
EP3750998A4 (en) 2022-02-23
RU2020128190A3 (https=) 2022-03-09
MX2020007929A (es) 2020-10-01
BR112020015238A2 (pt) 2020-12-29
CA3086618A1 (en) 2019-08-15
KR20200119237A (ko) 2020-10-19
TW201934752A (zh) 2019-09-01
AU2019218315A1 (en) 2020-07-09
US20210032307A1 (en) 2021-02-04
CN111094572A (zh) 2020-05-01
WO2019154311A1 (zh) 2019-08-15
RU2020128190A (ru) 2022-03-09
EP3750998A1 (en) 2020-12-16

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