JP2020530760A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2020530760A5 JP2020530760A5 JP2020500127A JP2020500127A JP2020530760A5 JP 2020530760 A5 JP2020530760 A5 JP 2020530760A5 JP 2020500127 A JP2020500127 A JP 2020500127A JP 2020500127 A JP2020500127 A JP 2020500127A JP 2020530760 A5 JP2020530760 A5 JP 2020530760A5
- Authority
- JP
- Japan
- Prior art keywords
- antibody
- cell
- domain
- heavy chain
- locus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000004027 cell Anatomy 0.000 claims 122
- 238000000034 method Methods 0.000 claims 43
- 239000000427 antigen Substances 0.000 claims 32
- 108091007433 antigens Proteins 0.000 claims 32
- 102000036639 antigens Human genes 0.000 claims 32
- 239000002773 nucleotide Substances 0.000 claims 24
- 125000003729 nucleotide group Chemical group 0.000 claims 24
- 239000003623 enhancer Substances 0.000 claims 21
- 241000251539 Vertebrata <Metazoa> Species 0.000 claims 19
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 10
- 108090000623 proteins and genes Proteins 0.000 claims 9
- 230000014509 gene expression Effects 0.000 claims 8
- 108010076504 Protein Sorting Signals Proteins 0.000 claims 7
- 210000004602 germ cell Anatomy 0.000 claims 6
- 238000004519 manufacturing process Methods 0.000 claims 6
- 230000035772 mutation Effects 0.000 claims 6
- 108020004707 nucleic acids Proteins 0.000 claims 6
- 150000007523 nucleic acids Chemical class 0.000 claims 6
- 102000039446 nucleic acids Human genes 0.000 claims 6
- 108700019146 Transgenes Proteins 0.000 claims 5
- 238000012258 culturing Methods 0.000 claims 5
- 241000894007 species Species 0.000 claims 5
- 230000006798 recombination Effects 0.000 claims 4
- 238000005215 recombination Methods 0.000 claims 4
- 210000003719 b-lymphocyte Anatomy 0.000 claims 3
- 210000004899 c-terminal region Anatomy 0.000 claims 3
- 230000001419 dependent effect Effects 0.000 claims 3
- 239000013604 expression vector Substances 0.000 claims 3
- 230000006801 homologous recombination Effects 0.000 claims 3
- 238000002744 homologous recombination Methods 0.000 claims 3
- 101000998950 Homo sapiens Immunoglobulin heavy variable 1-18 Proteins 0.000 claims 2
- 101000998952 Homo sapiens Immunoglobulin heavy variable 1-3 Proteins 0.000 claims 2
- 101000998947 Homo sapiens Immunoglobulin heavy variable 1-46 Proteins 0.000 claims 2
- 101000998951 Homo sapiens Immunoglobulin heavy variable 1-8 Proteins 0.000 claims 2
- 101001037138 Homo sapiens Immunoglobulin heavy variable 3-11 Proteins 0.000 claims 2
- 101001037136 Homo sapiens Immunoglobulin heavy variable 3-15 Proteins 0.000 claims 2
- 101001037142 Homo sapiens Immunoglobulin heavy variable 3-20 Proteins 0.000 claims 2
- 101001037141 Homo sapiens Immunoglobulin heavy variable 3-21 Proteins 0.000 claims 2
- 101001037140 Homo sapiens Immunoglobulin heavy variable 3-23 Proteins 0.000 claims 2
- 101001037139 Homo sapiens Immunoglobulin heavy variable 3-30 Proteins 0.000 claims 2
- 101001037143 Homo sapiens Immunoglobulin heavy variable 3-33 Proteins 0.000 claims 2
- 101000839662 Homo sapiens Immunoglobulin heavy variable 3-48 Proteins 0.000 claims 2
- 101000839660 Homo sapiens Immunoglobulin heavy variable 3-53 Proteins 0.000 claims 2
- 101001037153 Homo sapiens Immunoglobulin heavy variable 3-7 Proteins 0.000 claims 2
- 101001037144 Homo sapiens Immunoglobulin heavy variable 3-9 Proteins 0.000 claims 2
- 101000839682 Homo sapiens Immunoglobulin heavy variable 4-34 Proteins 0.000 claims 2
- 101000839679 Homo sapiens Immunoglobulin heavy variable 4-39 Proteins 0.000 claims 2
- 101000839686 Homo sapiens Immunoglobulin heavy variable 4-4 Proteins 0.000 claims 2
- 101000839781 Homo sapiens Immunoglobulin heavy variable 4-59 Proteins 0.000 claims 2
- 101000989076 Homo sapiens Immunoglobulin heavy variable 4-61 Proteins 0.000 claims 2
- 101000989062 Homo sapiens Immunoglobulin heavy variable 5-51 Proteins 0.000 claims 2
- 101000989060 Homo sapiens Immunoglobulin heavy variable 6-1 Proteins 0.000 claims 2
- 101000989065 Homo sapiens Immunoglobulin heavy variable 7-4-1 Proteins 0.000 claims 2
- 101000854886 Homo sapiens Immunoglobulin iota chain Proteins 0.000 claims 2
- 101001091242 Homo sapiens Immunoglobulin kappa joining 1 Proteins 0.000 claims 2
- 101001005365 Homo sapiens Immunoglobulin lambda variable 3-21 Proteins 0.000 claims 2
- 102100036884 Immunoglobulin heavy variable 1-18 Human genes 0.000 claims 2
- 102100036886 Immunoglobulin heavy variable 1-3 Human genes 0.000 claims 2
- 102100036888 Immunoglobulin heavy variable 1-46 Human genes 0.000 claims 2
- 102100036885 Immunoglobulin heavy variable 1-8 Human genes 0.000 claims 2
- 102100040222 Immunoglobulin heavy variable 3-11 Human genes 0.000 claims 2
- 102100040224 Immunoglobulin heavy variable 3-15 Human genes 0.000 claims 2
- 102100040218 Immunoglobulin heavy variable 3-20 Human genes 0.000 claims 2
- 102100040217 Immunoglobulin heavy variable 3-21 Human genes 0.000 claims 2
- 102100040220 Immunoglobulin heavy variable 3-23 Human genes 0.000 claims 2
- 102100040219 Immunoglobulin heavy variable 3-30 Human genes 0.000 claims 2
- 102100040236 Immunoglobulin heavy variable 3-33 Human genes 0.000 claims 2
- 102100028320 Immunoglobulin heavy variable 3-48 Human genes 0.000 claims 2
- 102100028317 Immunoglobulin heavy variable 3-53 Human genes 0.000 claims 2
- 102100040231 Immunoglobulin heavy variable 3-7 Human genes 0.000 claims 2
- 102100040234 Immunoglobulin heavy variable 3-9 Human genes 0.000 claims 2
- 102100028306 Immunoglobulin heavy variable 4-34 Human genes 0.000 claims 2
- 102100028312 Immunoglobulin heavy variable 4-39 Human genes 0.000 claims 2
- 102100028308 Immunoglobulin heavy variable 4-4 Human genes 0.000 claims 2
- 102100028405 Immunoglobulin heavy variable 4-59 Human genes 0.000 claims 2
- 102100029419 Immunoglobulin heavy variable 4-61 Human genes 0.000 claims 2
- 102100029414 Immunoglobulin heavy variable 5-51 Human genes 0.000 claims 2
- 102100029416 Immunoglobulin heavy variable 6-1 Human genes 0.000 claims 2
- 102100029420 Immunoglobulin heavy variable 7-4-1 Human genes 0.000 claims 2
- 102100020744 Immunoglobulin iota chain Human genes 0.000 claims 2
- 102100034892 Immunoglobulin kappa joining 1 Human genes 0.000 claims 2
- 102100025934 Immunoglobulin lambda variable 3-21 Human genes 0.000 claims 2
- 125000003275 alpha amino acid group Chemical group 0.000 claims 2
- 210000000601 blood cell Anatomy 0.000 claims 2
- 210000001185 bone marrow Anatomy 0.000 claims 2
- 239000003085 diluting agent Substances 0.000 claims 2
- 201000010099 disease Diseases 0.000 claims 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims 2
- 210000004408 hybridoma Anatomy 0.000 claims 2
- 239000008194 pharmaceutical composition Substances 0.000 claims 2
- 239000000546 pharmaceutical excipient Substances 0.000 claims 2
- 210000000952 spleen Anatomy 0.000 claims 2
- 238000013518 transcription Methods 0.000 claims 2
- 230000035897 transcription Effects 0.000 claims 2
- 230000009261 transgenic effect Effects 0.000 claims 2
- 239000013598 vector Substances 0.000 claims 2
- 108091026890 Coding region Proteins 0.000 claims 1
- 108020004414 DNA Proteins 0.000 claims 1
- 108700028146 Genetic Enhancer Elements Proteins 0.000 claims 1
- 101000839684 Homo sapiens Immunoglobulin heavy variable 4-31 Proteins 0.000 claims 1
- 101001138089 Homo sapiens Immunoglobulin kappa variable 1-39 Proteins 0.000 claims 1
- 101001047619 Homo sapiens Immunoglobulin kappa variable 3-20 Proteins 0.000 claims 1
- 102100028310 Immunoglobulin heavy variable 4-31 Human genes 0.000 claims 1
- 102100020910 Immunoglobulin kappa variable 1-39 Human genes 0.000 claims 1
- 102100022964 Immunoglobulin kappa variable 3-20 Human genes 0.000 claims 1
- 241000283984 Rodentia Species 0.000 claims 1
- 150000001413 amino acids Chemical class 0.000 claims 1
- 210000000628 antibody-producing cell Anatomy 0.000 claims 1
- 210000002459 blastocyst Anatomy 0.000 claims 1
- 230000001413 cellular effect Effects 0.000 claims 1
- 210000004978 chinese hamster ovary cell Anatomy 0.000 claims 1
- 239000013611 chromosomal DNA Substances 0.000 claims 1
- 230000004186 co-expression Effects 0.000 claims 1
- 230000037431 insertion Effects 0.000 claims 1
- 238000003780 insertion Methods 0.000 claims 1
- 210000001161 mammalian embryo Anatomy 0.000 claims 1
- 230000008707 rearrangement Effects 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB1710984.4 | 2017-07-07 | ||
| GBGB1710984.4A GB201710984D0 (en) | 2017-07-07 | 2017-07-07 | Cells, vertebrates, populations & methods |
| PCT/EP2018/068309 WO2019008123A2 (en) | 2017-07-07 | 2018-07-05 | CELLS, VERTEBRATES, POPULATIONS AND METHODS |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2020530760A JP2020530760A (ja) | 2020-10-29 |
| JP2020530760A5 true JP2020530760A5 (enExample) | 2021-08-12 |
| JP7288892B2 JP7288892B2 (ja) | 2023-06-08 |
Family
ID=59676641
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020500127A Active JP7288892B2 (ja) | 2017-07-07 | 2018-07-05 | 細胞、脊椎動物、集団及び方法 |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US20200214274A1 (enExample) |
| EP (2) | EP3648587B8 (enExample) |
| JP (1) | JP7288892B2 (enExample) |
| ES (1) | ES2986443T3 (enExample) |
| GB (1) | GB201710984D0 (enExample) |
| HU (1) | HUE068193T2 (enExample) |
| PL (1) | PL3648587T3 (enExample) |
| TW (1) | TWI820030B (enExample) |
| WO (1) | WO2019008123A2 (enExample) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3028565B1 (en) | 2009-07-08 | 2017-09-27 | Kymab Limited | Animal models and therapeutic molecules |
| EP2757875B2 (en) | 2011-09-19 | 2023-03-22 | Kymab Limited | Manipulation of immunoglobulin gene diversity and multi-antibody therapeutics |
| US10251377B2 (en) | 2012-03-28 | 2019-04-09 | Kymab Limited | Transgenic non-human vertebrate for the expression of class-switched, fully human, antibodies |
| GB2502127A (en) | 2012-05-17 | 2013-11-20 | Kymab Ltd | Multivalent antibodies and in vivo methods for their production |
| US9788534B2 (en) | 2013-03-18 | 2017-10-17 | Kymab Limited | Animal models and therapeutic molecules |
| US9783593B2 (en) | 2013-05-02 | 2017-10-10 | Kymab Limited | Antibodies, variable domains and chains tailored for human use |
| US11707056B2 (en) | 2013-05-02 | 2023-07-25 | Kymab Limited | Animals, repertoires and methods |
| WO2015049517A2 (en) | 2013-10-01 | 2015-04-09 | Kymab Limited | Animal models and therapeutic molecules |
| PL3720279T3 (pl) * | 2017-12-05 | 2023-01-16 | Regeneron Pharmaceuticals, Inc. | Myszy posiadające zmodyfikowany sposobami inżynierii łańcuch lekki lambda immunoglobuliny oraz ich zastosowania |
| AU2020226445B2 (en) | 2019-02-18 | 2025-05-29 | Biocytogen Pharmaceuticals (Beijing) Co., Ltd. | Genetically modified non-human animals with humanized immunoglobulin locus |
| JP2022532388A (ja) * | 2019-05-15 | 2022-07-14 | カイマブ・リミテッド | 改善されたラムダ抗体 |
| GB2590642B (en) * | 2019-12-20 | 2024-02-14 | Kymab Ltd | Improved lambda antibodies |
| CA3136478A1 (en) * | 2019-06-05 | 2020-12-10 | Regeneron Pharmaceuticals, Inc. | Non-human animals having a limited lambda light chain repertoire expressed from the kappa locus and uses thereof |
| AU2021283564B2 (en) * | 2020-06-02 | 2025-05-29 | Biocytogen Pharmaceuticals (Beijing) Co., Ltd. | Genetically modified non-human animals with common light chain immunoglobulin locus |
| EP4211155A1 (en) | 2020-09-11 | 2023-07-19 | Regeneron Pharmaceuticals, Inc. | Identification and production of antigen-specific antibodies |
| EP4262373A1 (en) | 2020-12-16 | 2023-10-25 | Regeneron Pharmaceuticals, Inc. | Mice expressing humanized fc alpha receptors |
| GB202107372D0 (en) | 2021-05-24 | 2021-07-07 | Petmedix Ltd | Animal models and therapeutics molecules |
| GB202209247D0 (en) | 2022-06-23 | 2022-08-10 | Petmedix Ltd | Animal models and therapeutic molecules |
| GB202216503D0 (en) | 2022-11-05 | 2022-12-21 | Quadrucept Bio Ltd | Non-human vertebrates & cells |
| GB202217978D0 (en) | 2022-11-30 | 2023-01-11 | Petmedix Ltd | Rodents expressing a common light chain |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7041871B1 (en) * | 1995-10-10 | 2006-05-09 | Genpharm International, Inc. | Transgenic non-human animals capable of producing heterologous antibodies |
| US5731168A (en) | 1995-03-01 | 1998-03-24 | Genentech, Inc. | Method for making heteromultimeric polypeptides |
| EP0979281B1 (en) | 1997-05-02 | 2005-07-20 | Genentech, Inc. | A method for making multispecific antibodies having heteromultimeric and common components |
| SI1523496T1 (sl) * | 2002-07-18 | 2011-11-30 | Merus B V | Rekombinantno proizvajanje zmesi protiteles |
| EP2395017A3 (en) | 2003-05-30 | 2012-12-19 | Merus B.V. | Design and use of paired variable regions of specific binding molecules |
| WO2006117699A2 (en) * | 2005-04-29 | 2006-11-09 | Innate Pharma | Transgenic animals and methods of making recombinant antibodies |
| RU2559524C2 (ru) * | 2008-06-27 | 2015-08-10 | Мерюс Б.В. | Продуцирующие антитела млекопитающие, не являющиеся человеком |
| US20120021409A1 (en) * | 2010-02-08 | 2012-01-26 | Regeneron Pharmaceuticals, Inc. | Common Light Chain Mouse |
| SMT201900372T1 (it) * | 2010-02-08 | 2019-09-09 | Regeneron Pharma | Topo con catena leggera comune |
| US9253965B2 (en) * | 2012-03-28 | 2016-02-09 | Kymab Limited | Animal models and therapeutic molecules |
| RS57582B1 (sr) * | 2013-02-20 | 2018-11-30 | Regeneron Pharma | Nehumane životinje sa modifikovanim sekvencama imunoglobulinskog teškog lanca |
| RU2015143108A (ru) | 2013-03-13 | 2017-04-20 | Регенерон Фарматютикалз, Инк. | Мышь с общей легкой цепью |
| GB201316644D0 (en) | 2013-09-19 | 2013-11-06 | Kymab Ltd | Expression vector production & High-Throughput cell screening |
-
2017
- 2017-07-07 GB GBGB1710984.4A patent/GB201710984D0/en not_active Ceased
-
2018
- 2018-07-05 EP EP18743421.2A patent/EP3648587B8/en active Active
- 2018-07-05 JP JP2020500127A patent/JP7288892B2/ja active Active
- 2018-07-05 WO PCT/EP2018/068309 patent/WO2019008123A2/en not_active Ceased
- 2018-07-05 PL PL18743421.2T patent/PL3648587T3/pl unknown
- 2018-07-05 HU HUE18743421A patent/HUE068193T2/hu unknown
- 2018-07-05 ES ES18743421T patent/ES2986443T3/es active Active
- 2018-07-05 EP EP24178098.0A patent/EP4410986A3/en active Pending
- 2018-07-06 TW TW107123525A patent/TWI820030B/zh active
-
2019
- 2019-12-19 US US16/721,326 patent/US20200214274A1/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2020530760A5 (enExample) | ||
| US20240057572A1 (en) | Animal Models and Therapeutic Molecules | |
| KR102116296B1 (ko) | 일반적인 경쇄 마우스 | |
| HUE030208T2 (en) | Mice that make heavy chain antibodies | |
| US20150033372A1 (en) | Human VpreB & Chimaeric Surrogate Light Chains in Transgenic Non-Human Vertebrates | |
| RU2014153673A (ru) | Гуманизированные не относящиеся к человеку животные с ограниченными локусами тяжелой цепи иммуноглобулина | |
| KR20110089846A (ko) | 키메라 항체의 제조를 위한 인간 이외의 포유동물 | |
| US20250234846A1 (en) | Genetically modified non-human mammals, preparation methods therefor, and applications thereof | |
| US20240206440A1 (en) | Animal models and therapeutic molecules | |
| EP4606901A1 (en) | Animal model for producing humanized antibody, and construction method therefor | |
| US12408640B2 (en) | Transgenic animal for producing diversified antibodies that have the same light chain I | |
| KR20250022132A (ko) | 동물 모델과 치료 분자 | |
| GB2495083A (en) | Human VpreB and chimaeric surrogate light chains in transgenic non-human vertebrates |