JP2020505000A5 - - Google Patents
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- JP2020505000A5 JP2020505000A5 JP2019532936A JP2019532936A JP2020505000A5 JP 2020505000 A5 JP2020505000 A5 JP 2020505000A5 JP 2019532936 A JP2019532936 A JP 2019532936A JP 2019532936 A JP2019532936 A JP 2019532936A JP 2020505000 A5 JP2020505000 A5 JP 2020505000A5
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- nucleic acid
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- 108090000623 proteins and genes Proteins 0.000 claims description 112
- 102000004169 proteins and genes Human genes 0.000 claims description 110
- 150000007523 nucleic acids Chemical group 0.000 claims description 72
- 102000040430 polynucleotide Human genes 0.000 claims description 49
- 108091033319 polynucleotide Proteins 0.000 claims description 49
- 239000002157 polynucleotide Substances 0.000 claims description 49
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 48
- 210000004027 cell Anatomy 0.000 claims description 45
- 238000000034 method Methods 0.000 claims description 34
- 238000012986 modification Methods 0.000 claims description 34
- 230000004048 modification Effects 0.000 claims description 34
- 108020004414 DNA Proteins 0.000 claims description 33
- 238000003776 cleavage reaction Methods 0.000 claims description 31
- 230000007017 scission Effects 0.000 claims description 31
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 29
- 230000027455 binding Effects 0.000 claims description 29
- 238000009739 binding Methods 0.000 claims description 29
- 238000002372 labelling Methods 0.000 claims description 29
- 230000008685 targeting Effects 0.000 claims description 29
- 102000007999 Nuclear Proteins Human genes 0.000 claims description 28
- 108010089610 Nuclear Proteins Proteins 0.000 claims description 28
- 101710163270 Nuclease Proteins 0.000 claims description 23
- 102000011931 Nucleoproteins Human genes 0.000 claims description 21
- 108010061100 Nucleoproteins Proteins 0.000 claims description 21
- 102000053602 DNA Human genes 0.000 claims description 20
- 241000894006 Bacteria Species 0.000 claims description 13
- 125000003729 nucleotide group Chemical group 0.000 claims description 13
- 108010043121 Green Fluorescent Proteins Proteins 0.000 claims description 10
- 102000004144 Green Fluorescent Proteins Human genes 0.000 claims description 10
- 239000005090 green fluorescent protein Substances 0.000 claims description 10
- 210000005260 human cell Anatomy 0.000 claims description 10
- 239000003550 marker Substances 0.000 claims description 9
- 102000039446 nucleic acids Human genes 0.000 claims description 9
- 108020004707 nucleic acids Proteins 0.000 claims description 9
- 108010013043 Acetylesterase Proteins 0.000 claims description 8
- 108091033409 CRISPR Proteins 0.000 claims description 8
- 102100025169 Max-binding protein MNT Human genes 0.000 claims description 8
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 8
- 108091006107 transcriptional repressors Proteins 0.000 claims description 8
- 230000000295 complement effect Effects 0.000 claims description 7
- 230000000694 effects Effects 0.000 claims description 7
- 230000030279 gene silencing Effects 0.000 claims description 7
- 108060004795 Methyltransferase Proteins 0.000 claims description 6
- -1 optionally Proteins 0.000 claims description 6
- 241000894007 species Species 0.000 claims description 6
- 241000203069 Archaea Species 0.000 claims description 5
- 102000052510 DNA-Binding Proteins Human genes 0.000 claims description 5
- 101710096438 DNA-binding protein Proteins 0.000 claims description 5
- 108010036115 Histone Methyltransferases Proteins 0.000 claims description 5
- 102000011787 Histone Methyltransferases Human genes 0.000 claims description 5
- 108700019535 Phosphoprotein Phosphatases Proteins 0.000 claims description 5
- 102000045595 Phosphoprotein Phosphatases Human genes 0.000 claims description 5
- 102000001253 Protein Kinase Human genes 0.000 claims description 5
- 108010076504 Protein Sorting Signals Proteins 0.000 claims description 5
- 108091028113 Trans-activating crRNA Proteins 0.000 claims description 5
- 241000700605 Viruses Species 0.000 claims description 5
- 239000012190 activator Substances 0.000 claims description 5
- 238000001261 affinity purification Methods 0.000 claims description 5
- 102000023732 binding proteins Human genes 0.000 claims description 5
- 108091008324 binding proteins Proteins 0.000 claims description 5
- 108091006047 fluorescent proteins Proteins 0.000 claims description 5
- 102000034287 fluorescent proteins Human genes 0.000 claims description 5
- 238000012226 gene silencing method Methods 0.000 claims description 5
- 239000003446 ligand Substances 0.000 claims description 5
- 108060006633 protein kinase Proteins 0.000 claims description 5
- 230000002103 transcriptional effect Effects 0.000 claims description 5
- 238000010354 CRISPR gene editing Methods 0.000 claims description 4
- 239000013604 expression vector Substances 0.000 claims description 4
- 210000001236 prokaryotic cell Anatomy 0.000 claims description 4
- 241001468175 Geobacillus thermodenitrificans Species 0.000 claims description 2
- 125000006850 spacer group Chemical group 0.000 claims description 2
- 230000030570 cellular localization Effects 0.000 claims 1
- 239000002773 nucleotide Substances 0.000 description 7
- 230000014509 gene expression Effects 0.000 description 6
- 241000626621 Geobacillus Species 0.000 description 5
- 210000004899 c-terminal region Anatomy 0.000 description 5
- 230000005782 double-strand break Effects 0.000 description 4
- 230000003834 intracellular effect Effects 0.000 description 4
- 230000007018 DNA scission Effects 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 230000037430 deletion Effects 0.000 description 3
- 238000012217 deletion Methods 0.000 description 3
- 230000037431 insertion Effects 0.000 description 3
- 238000003780 insertion Methods 0.000 description 3
- 230000005783 single-strand break Effects 0.000 description 3
- 108091006106 transcriptional activators Proteins 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 2
- 210000003527 eukaryotic cell Anatomy 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 108010008532 Deoxyribonuclease I Proteins 0.000 description 1
- 102000007260 Deoxyribonuclease I Human genes 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 230000009918 complex formation Effects 0.000 description 1
- 238000010362 genome editing Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2022121297A JP2022166045A (ja) | 2016-12-14 | 2022-07-29 | 熱安定性ｃａｓ９ヌクレアーゼ |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EPPCT/EP2016/081077 | 2016-12-14 | ||
| PCT/EP2016/081077 WO2018108272A1 (en) | 2016-12-14 | 2016-12-14 | Thermostable cas9 nucleases |
| PCT/EP2017/070796 WO2018108338A1 (en) | 2016-12-14 | 2017-08-16 | Thermostable cas9 nucleases |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022121297A Division JP2022166045A (ja) | 2016-12-14 | 2022-07-29 | 熱安定性ｃａｓ９ヌクレアーゼ |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2020505000A JP2020505000A (ja) | 2020-02-20 |
| JP2020505000A5 true JP2020505000A5 (enExample) | 2020-09-24 |
| JP7223376B2 JP7223376B2 (ja) | 2023-02-16 |
Family
ID=57755254
Family Applications (6)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019533096A Active JP7182545B2 (ja) | 2016-12-14 | 2016-12-14 | 熱安定性ｃａｓ９ヌクレアーゼ |
| JP2019533103A Active JP7223377B2 (ja) | 2016-12-14 | 2017-08-16 | 熱安定性ｃａｓ９ヌクレアーゼ |
| JP2019532936A Active JP7223376B2 (ja) | 2016-12-14 | 2017-08-16 | 熱安定性ｃａｓ９ヌクレアーゼ |
| JP2019533098A Active JP7283698B2 (ja) | 2016-12-14 | 2017-12-14 | 熱安定性ｃａｓ９ヌクレアーゼ |
| JP2022121297A Withdrawn JP2022166045A (ja) | 2016-12-14 | 2022-07-29 | 熱安定性ｃａｓ９ヌクレアーゼ |
| JP2022128902A Withdrawn JP2022166170A (ja) | 2016-12-14 | 2022-08-12 | 熱安定性ｃａｓ９ヌクレアーゼ |
Family Applications Before (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019533096A Active JP7182545B2 (ja) | 2016-12-14 | 2016-12-14 | 熱安定性ｃａｓ９ヌクレアーゼ |
| JP2019533103A Active JP7223377B2 (ja) | 2016-12-14 | 2017-08-16 | 熱安定性ｃａｓ９ヌクレアーゼ |
Family Applications After (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019533098A Active JP7283698B2 (ja) | 2016-12-14 | 2017-12-14 | 熱安定性ｃａｓ９ヌクレアーゼ |
| JP2022121297A Withdrawn JP2022166045A (ja) | 2016-12-14 | 2022-07-29 | 熱安定性ｃａｓ９ヌクレアーゼ |
| JP2022128902A Withdrawn JP2022166170A (ja) | 2016-12-14 | 2022-08-12 | 熱安定性ｃａｓ９ヌクレアーゼ |
Country Status (11)
| Country | Link |
|---|---|
| US (5) | US11242513B2 (enExample) |
| EP (4) | EP3555275A1 (enExample) |
| JP (6) | JP7182545B2 (enExample) |
| KR (3) | KR102523543B1 (enExample) |
| CN (4) | CN110312792B (enExample) |
| AU (4) | AU2016432443B2 (enExample) |
| BR (3) | BR112019012155A2 (enExample) |
| CA (4) | CA3046824A1 (enExample) |
| EA (4) | EA201991443A1 (enExample) |
| PH (4) | PH12019501346A1 (enExample) |
| WO (3) | WO2018108272A1 (enExample) |
Families Citing this family (67)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10323236B2 (en) | 2011-07-22 | 2019-06-18 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| US9637739B2 (en) * | 2012-03-20 | 2017-05-02 | Vilnius University | RNA-directed DNA cleavage by the Cas9-crRNA complex |
| US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9340800B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | Extended DNA-sensing GRNAS |
| US9737604B2 (en) | 2013-09-06 | 2017-08-22 | President And Fellows Of Harvard College | Use of cationic lipids to deliver CAS9 |
| US9388430B2 (en) | 2013-09-06 | 2016-07-12 | President And Fellows Of Harvard College | Cas9-recombinase fusion proteins and uses thereof |
| US20150166984A1 (en) | 2013-12-12 | 2015-06-18 | President And Fellows Of Harvard College | Methods for correcting alpha-antitrypsin point mutations |
| US10077453B2 (en) | 2014-07-30 | 2018-09-18 | President And Fellows Of Harvard College | CAS9 proteins including ligand-dependent inteins |
| US20180250424A1 (en) | 2014-10-10 | 2018-09-06 | Editas Medicine, Inc. | Compositions and methods for promoting homology directed repair |
| EP4464338A3 (en) | 2014-11-07 | 2025-02-12 | Editas Medicine, Inc. | Systems for improving crispr/cas-mediated genome-editing |
| JP7030522B2 (ja) | 2015-05-11 | 2022-03-07 | エディタス・メディシン、インコーポレイテッド | 幹細胞における遺伝子編集のための最適化ｃｒｉｓｐｒ／ｃａｓ９システムおよび方法 |
| JP7396783B2 (ja) | 2015-06-09 | 2023-12-12 | エディタス・メディシン、インコーポレイテッド | 移植を改善するためのｃｒｉｓｐｒ／ｃａｓ関連方法および組成物 |
| GB201510296D0 (en) * | 2015-06-12 | 2015-07-29 | Univ Wageningen | Thermostable CAS9 nucleases |
| EP3786294A1 (en) | 2015-09-24 | 2021-03-03 | Editas Medicine, Inc. | Use of exonucleases to improve crispr/cas-mediated genome editing |
| US12043852B2 (en) | 2015-10-23 | 2024-07-23 | President And Fellows Of Harvard College | Evolved Cas9 proteins for gene editing |
| WO2017165826A1 (en) | 2016-03-25 | 2017-09-28 | Editas Medicine, Inc. | Genome editing systems comprising repair-modulating enzyme molecules and methods of their use |
| EP4047092B1 (en) | 2016-04-13 | 2025-07-30 | Editas Medicine, Inc. | Cas9 fusion molecules, gene editing systems, and methods of use thereof |
| CA3032699A1 (en) | 2016-08-03 | 2018-02-08 | President And Fellows Of Harvard College | Adenosine nucleobase editors and uses thereof |
| CA3033327A1 (en) | 2016-08-09 | 2018-02-15 | President And Fellows Of Harvard College | Programmable cas9-recombinase fusion proteins and uses thereof |
| WO2018039438A1 (en) | 2016-08-24 | 2018-03-01 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| US11306324B2 (en) | 2016-10-14 | 2022-04-19 | President And Fellows Of Harvard College | AAV delivery of nucleobase editors |
| EA201991443A1 (ru) | 2016-12-14 | 2020-01-14 | Вагенинген Университейт | ТЕРМОСТАБИЛЬНЫЕ НУКЛЕАЗЫ Cas9 |
| US12286727B2 (en) | 2016-12-19 | 2025-04-29 | Editas Medicine, Inc. | Assessing nuclease cleavage |
| WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
| EP3565907B1 (en) | 2017-01-06 | 2022-05-04 | Editas Medicine, Inc. | Methods of assessing nuclease cleavage |
| EP3592853A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Suppression of pain by gene editing |
| JP2020510038A (ja) | 2017-03-09 | 2020-04-02 | プレジデント アンド フェローズ オブ ハーバード カレッジ | がんワクチン |
| CN110914310A (zh) | 2017-03-10 | 2020-03-24 | 哈佛大学的校长及成员们 | 胞嘧啶至鸟嘌呤碱基编辑器 |
| EP3601562A1 (en) | 2017-03-23 | 2020-02-05 | President and Fellows of Harvard College | Nucleobase editors comprising nucleic acid programmable dna binding proteins |
| EP3615672A1 (en) | 2017-04-28 | 2020-03-04 | Editas Medicine, Inc. | Methods and systems for analyzing guide rna molecules |
| US11560566B2 (en) | 2017-05-12 | 2023-01-24 | President And Fellows Of Harvard College | Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation |
| WO2018227114A1 (en) | 2017-06-09 | 2018-12-13 | Editas Medicine, Inc. | Engineered cas9 nucleases |
| US11866726B2 (en) | 2017-07-14 | 2024-01-09 | Editas Medicine, Inc. | Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites |
| JP2020534795A (ja) | 2017-07-28 | 2020-12-03 | プレジデント アンド フェローズ オブ ハーバード カレッジ | ファージによって支援される連続的進化（ｐａｃｅ）を用いて塩基編集因子を進化させるための方法および組成物 |
| US11319532B2 (en) | 2017-08-30 | 2022-05-03 | President And Fellows Of Harvard College | High efficiency base editors comprising Gam |
| GB201716590D0 (en) * | 2017-10-10 | 2017-11-22 | Univ Wageningen | Thermostable cas9 nucleases with reduced off-target activity |
| JP2021500036A (ja) | 2017-10-16 | 2021-01-07 | ザ ブロード インスティテュート, インコーポレーテッドThe Broad Institute, Inc. | アデノシン塩基編集因子の使用 |
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| AU2019380672A1 (en) * | 2018-11-16 | 2021-05-27 | Depixus | Optimization of In Vitro Isolation Of Nucleic Acids Using Site-Specific Nucleases |
| RU2712497C1 (ru) * | 2018-11-26 | 2020-01-29 | Автономная некоммерческая образовательная организация высшего образования Сколковский институт науки и технологий | Средство разрезания ДНК на основе Cas9 белка из биотехнологически значимой бактерии Clostridium cellulolyticum |
| WO2020154500A1 (en) | 2019-01-23 | 2020-07-30 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| SG11202108812SA (en) | 2019-02-15 | 2021-09-29 | Sigma Aldrich Co Llc | Crispr/cas fusion proteins and systems |
| KR20210143230A (ko) | 2019-03-19 | 2021-11-26 | 더 브로드 인스티튜트, 인코퍼레이티드 | 뉴클레오티드 서열을 편집하기 위한 방법 및 조성물 |
| WO2020214842A1 (en) | 2019-04-17 | 2020-10-22 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| CN112301018B (zh) * | 2019-05-14 | 2023-07-25 | 深圳华大生命科学研究院 | 新型的Cas蛋白、Crispr-Cas系统及其在基因编辑领域中的用途 |
| WO2021017200A1 (zh) * | 2019-07-30 | 2021-02-04 | 湖北大学 | 一种基于运动发酵单胞菌的CRISPR-Cas系统、基因组编辑体系及其应用 |
| CN110331158B (zh) * | 2019-07-30 | 2021-09-14 | 湖北大学 | 基于运动发酵单胞菌内源CRISPR-Cas系统的多基因位点同时编辑方法及其应用 |
| WO2021032180A1 (zh) * | 2019-08-21 | 2021-02-25 | 江南大学 | 一种多靶点编辑重组曲霉菌株的可视化筛选方法 |
| US11879134B1 (en) * | 2019-09-05 | 2024-01-23 | The Regents Of The University Of Colorado, A Body Corporate | Recombineering machinery to increase homology directed genome editing in thermophilic microbes |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
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| CN111778230A (zh) * | 2020-07-17 | 2020-10-16 | 山东舜丰生物科技有限公司 | 一种适用于Cas12蛋白的缓冲系统及其应用 |
| RU2749307C1 (ru) * | 2020-10-30 | 2021-06-08 | Федеральное государственное бюджетное научное учреждение "Всероссийский научно-исследовательский институт сельскохозяйственной биотехнологии" (ФГБНУ ВНИИСБ) | Новая компактная нуклеаза CAS9 II типа из Anoxybacillus flavithermus |
| CN116334037A (zh) * | 2020-11-11 | 2023-06-27 | 山东舜丰生物科技有限公司 | 新型Cas酶和系统以及应用 |
| CN114480347B (zh) * | 2022-02-21 | 2022-12-23 | 中国科学院地球化学研究所 | 一种纯化Cas12a蛋白的方法 |
| CN114934031B (zh) * | 2022-05-25 | 2023-08-01 | 广州瑞风生物科技有限公司 | 新型Cas效应蛋白、基因编辑系统及用途 |
| JP7152094B1 (ja) | 2022-06-30 | 2022-10-12 | リージョナルフィッシュ株式会社 | ｔｒａｃｒＲＮＡユニット、及びゲノム編集方法 |
| CN116083400B (zh) * | 2022-12-08 | 2023-12-12 | 广州瑞风生物科技有限公司 | Cas蛋白截短体、构建其的方法及其应用 |
| CN116144631B (zh) * | 2023-01-17 | 2023-09-15 | 华中农业大学 | 耐热型核酸内切酶及其介导的基因编辑系统 |
| CN118773168B (zh) * | 2023-11-24 | 2025-07-25 | 华智生物技术有限公司 | CRISPR/Cas效应蛋白HT001及系统与应用 |
| US20250250641A1 (en) * | 2024-02-06 | 2025-08-07 | Florida State University Research Foundation, Inc. | Methods and compositions related to epigenetic editing by a methlylation-dependent cas9 |
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| CN107709562A (zh) * | 2015-05-15 | 2018-02-16 | 先锋国际良种公司 | 指导rna/cas内切核酸酶系统 |
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