CN110312792B - 热稳定的Cas9核酸酶 - Google Patents

热稳定的Cas9核酸酶 Download PDF

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CN110312792B
CN110312792B CN201680092098.5A CN201680092098A CN110312792B CN 110312792 B CN110312792 B CN 110312792B CN 201680092098 A CN201680092098 A CN 201680092098A CN 110312792 B CN110312792 B CN 110312792B
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protein
nucleic acid
double
target nucleic
sequence
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CN110312792A (zh
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约翰·万德奥斯特
理查德·范克拉嫩堡
艾勒克·芬纳·博斯马
扬尼斯·莫加科斯
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Stichting voor de Technische Wetenschappen STW
Wageningen Universiteit
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
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    • C12N15/90—Stable introduction of foreign DNA into chromosome
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

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CN201680092098.5A 2016-12-14 2016-12-14 热稳定的Cas9核酸酶 Active CN110312792B (zh)

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Application Number Priority Date Filing Date Title
PCT/EP2016/081077 WO2018108272A1 (en) 2016-12-14 2016-12-14 Thermostable cas9 nucleases

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CN110312792A CN110312792A (zh) 2019-10-08
CN110312792B true CN110312792B (zh) 2024-04-09

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CN201680092098.5A Active CN110312792B (zh) 2016-12-14 2016-12-14 热稳定的Cas9核酸酶
CN201780086541.2A Active CN110352241B (zh) 2016-12-14 2017-08-16 热稳定的Cas9核酸酶
CN201780086540.8A Active CN110382693B (zh) 2016-12-14 2017-08-16 热稳定的Cas9核酸酶
CN201780086546.5A Active CN110431229B (zh) 2016-12-14 2017-12-14 热稳定的Cas9核酸酶

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CN201780086540.8A Active CN110382693B (zh) 2016-12-14 2017-08-16 热稳定的Cas9核酸酶
CN201780086546.5A Active CN110431229B (zh) 2016-12-14 2017-12-14 热稳定的Cas9核酸酶

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US (5) US11242513B2 (enExample)
EP (4) EP3555275A1 (enExample)
JP (6) JP7182545B2 (enExample)
KR (3) KR102523543B1 (enExample)
CN (4) CN110312792B (enExample)
AU (4) AU2016432443B2 (enExample)
BR (3) BR112019012155A2 (enExample)
CA (4) CA3046824A1 (enExample)
EA (4) EA201991443A1 (enExample)
PH (4) PH12019501335A1 (enExample)
WO (3) WO2018108272A1 (enExample)

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CN113881652B (zh) * 2020-11-11 2022-11-22 山东舜丰生物科技有限公司 新型Cas酶和系统以及应用
CN114480347B (zh) * 2022-02-21 2022-12-23 中国科学院地球化学研究所 一种纯化Cas12a蛋白的方法
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JP7152094B1 (ja) * 2022-06-30 2022-10-12 リージョナルフィッシュ株式会社 ｔｒａｃｒＲＮＡユニット、及びゲノム編集方法
CN117701530A (zh) * 2022-12-08 2024-03-15 广州瑞风生物科技有限公司 Cas蛋白截短体、构建其的方法及其应用
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WO2025107617A1 (zh) * 2023-11-24 2025-05-30 华智生物技术有限公司 CRISPR/Cas效应蛋白及系统
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