JP2018515453A5 - - Google Patents

Info

Publication number

JP2018515453A5

JP2018515453A5 JP2017555395A JP2017555395A JP2018515453A5 JP 2018515453 A5 JP2018515453 A5 JP 2018515453A5 JP 2017555395 A JP2017555395 A JP 2017555395A JP 2017555395 A JP2017555395 A JP 2017555395A JP 2018515453 A5 JP2018515453 A5 JP 2018515453A5

Authority

JP

Japan

Prior art keywords

reducing agent

variable domain

antibody

range

seq

Prior art date

2016-04-22

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
• Global Dossier
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• 238000000034 method Methods 0.000 claims 29
• 239000003638 chemical reducing agent Substances 0.000 claims 10
• 239000000427 antigen Substances 0.000 claims 8
• 102000036639 antigens Human genes 0.000 claims 8
• 108091007433 antigens Proteins 0.000 claims 8
• DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 claims 8
• RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 claims 4
• 229940024606 amino acid Drugs 0.000 claims 3
• 150000001413 amino acids Chemical class 0.000 claims 3
• 101000679851 Homo sapiens Tumor necrosis factor receptor superfamily member 4 Proteins 0.000 claims 2
• 102000008100 Human Serum Albumin Human genes 0.000 claims 2
• 108091006905 Human Serum Albumin Proteins 0.000 claims 2
• 238000004587 chromatography analysis Methods 0.000 claims 2
• UFULAYFCSOUIOV-UHFFFAOYSA-N cysteamine Chemical compound NCCS UFULAYFCSOUIOV-UHFFFAOYSA-N 0.000 claims 2
• VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 claims 2
• 229960003180 glutathione Drugs 0.000 claims 2
• 102000050320 human TNFRSF4 Human genes 0.000 claims 2
• 229960003151 mercaptamine Drugs 0.000 claims 2
• FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 1
• 239000004475 Arginine Substances 0.000 claims 1
• 108010024636 Glutathione Proteins 0.000 claims 1
• XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 claims 1
• ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 claims 1
• ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 claims 1
• KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 claims 1
• KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims 1
• 239000004472 Lysine Substances 0.000 claims 1
• ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 claims 1
• 238000013019 agitation Methods 0.000 claims 1
• ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims 1
• 238000011210 chromatographic step Methods 0.000 claims 1
• 230000000295 complement effect Effects 0.000 claims 1
• 229960002433 cysteine Drugs 0.000 claims 1
• NVJBFARDFTXOTO-UHFFFAOYSA-N diethyl sulfite Chemical compound CCOS(=O)OCC NVJBFARDFTXOTO-UHFFFAOYSA-N 0.000 claims 1
• 238000011143 downstream manufacturing Methods 0.000 claims 1
• 108020001507 fusion proteins Proteins 0.000 claims 1
• 102000037865 fusion proteins Human genes 0.000 claims 1
• 238000004191 hydrophobic interaction chromatography Methods 0.000 claims 1
• 238000004255 ion exchange chromatography Methods 0.000 claims 1
• 239000000178 monomer Substances 0.000 claims 1
• 238000000746 purification Methods 0.000 claims 1
• 238000003756 stirring Methods 0.000 claims 1