JP2018515449A5 - - Google Patents

Info

Publication number

JP2018515449A5

JP2018515449A5 JP2017555223A JP2017555223A JP2018515449A5 JP 2018515449 A5 JP2018515449 A5 JP 2018515449A5 JP 2017555223 A JP2017555223 A JP 2017555223A JP 2017555223 A JP2017555223 A JP 2017555223A JP 2018515449 A5 JP2018515449 A5 JP 2018515449A5

Authority

JP

Japan

Prior art keywords

variable domain

chain variable

antibody

mercaptoethylamine

seq

Prior art date

2016-04-22

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
• Global Dossier
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• 238000000034 method Methods 0.000 claims 21
• 239000000427 antigen Substances 0.000 claims 10
• 102000036639 antigens Human genes 0.000 claims 10
• 108091007433 antigens Proteins 0.000 claims 10
• UFULAYFCSOUIOV-UHFFFAOYSA-N cysteamine Chemical compound NCCS UFULAYFCSOUIOV-UHFFFAOYSA-N 0.000 claims 8
• 229960003151 mercaptamine Drugs 0.000 claims 8
• XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims 7
• 239000004202 carbamide Substances 0.000 claims 7
• 229960000789 guanidine hydrochloride Drugs 0.000 claims 4
• PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 claims 4
• 239000003638 chemical reducing agent Substances 0.000 claims 3
• 239000003398 denaturant Substances 0.000 claims 3
• 239000000178 monomer Substances 0.000 claims 3
• 101000679851 Homo sapiens Tumor necrosis factor receptor superfamily member 4 Proteins 0.000 claims 2
• 102000008100 Human Serum Albumin Human genes 0.000 claims 2
• 108091006905 Human Serum Albumin Proteins 0.000 claims 2
• 238000006243 chemical reaction Methods 0.000 claims 2
• 102000050320 human TNFRSF4 Human genes 0.000 claims 2
• 239000003607 modifier Substances 0.000 claims 2
• 241000894007 species Species 0.000 claims 2
• FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 1
• 238000013019 agitation Methods 0.000 claims 1
• 238000004587 chromatography analysis Methods 0.000 claims 1
• 230000000295 complement effect Effects 0.000 claims 1
• 238000011143 downstream manufacturing Methods 0.000 claims 1
• 238000012444 downstream purification process Methods 0.000 claims 1
• 108020001507 fusion proteins Proteins 0.000 claims 1
• 102000037865 fusion proteins Human genes 0.000 claims 1
• 238000003756 stirring Methods 0.000 claims 1