JP2018506307A5 - - Google Patents
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- Publication number
- JP2018506307A5 JP2018506307A5 JP2017560886A JP2017560886A JP2018506307A5 JP 2018506307 A5 JP2018506307 A5 JP 2018506307A5 JP 2017560886 A JP2017560886 A JP 2017560886A JP 2017560886 A JP2017560886 A JP 2017560886A JP 2018506307 A5 JP2018506307 A5 JP 2018506307A5
- Authority
- JP
- Japan
- Prior art keywords
- target
- specific amplification
- amplification products
- resolvase
- specific
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 230000003321 amplification Effects 0.000 claims description 103
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 103
- 238000000034 method Methods 0.000 claims description 68
- 102000018120 Recombinases Human genes 0.000 claims description 37
- 108010091086 Recombinases Proteins 0.000 claims description 37
- 230000002159 abnormal effect Effects 0.000 claims description 17
- 238000006243 chemical reaction Methods 0.000 claims description 14
- 108020004707 nucleic acids Proteins 0.000 claims description 14
- 102000039446 nucleic acids Human genes 0.000 claims description 14
- 150000007523 nucleic acids Chemical class 0.000 claims description 14
- 108060002716 Exonuclease Proteins 0.000 claims description 12
- 102000013165 exonuclease Human genes 0.000 claims description 12
- 108010064144 endodeoxyribonuclease VII Proteins 0.000 claims description 11
- 230000001419 dependent effect Effects 0.000 claims description 9
- 108091033319 polynucleotide Proteins 0.000 claims description 7
- 102000040430 polynucleotide Human genes 0.000 claims description 7
- 239000002157 polynucleotide Substances 0.000 claims description 7
- 108010008532 Deoxyribonuclease I Proteins 0.000 claims description 4
- 102000007260 Deoxyribonuclease I Human genes 0.000 claims description 4
- 238000003752 polymerase chain reaction Methods 0.000 claims description 4
- 108010007577 Exodeoxyribonuclease I Proteins 0.000 claims description 3
- 239000002299 complementary DNA Substances 0.000 claims description 3
- 238000007403 mPCR Methods 0.000 claims description 3
- 108020004414 DNA Proteins 0.000 description 30
- 239000000203 mixture Substances 0.000 description 9
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 2
- RYYWUUFWQRZTIU-UHFFFAOYSA-N Thiophosphoric acid Chemical class OP(O)(S)=O RYYWUUFWQRZTIU-UHFFFAOYSA-N 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000007481 next generation sequencing Methods 0.000 description 2
- 108091093088 Amplicon Proteins 0.000 description 1
- 102000012410 DNA Ligases Human genes 0.000 description 1
- 108010061982 DNA Ligases Proteins 0.000 description 1
- 102100029075 Exonuclease 1 Human genes 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562114788P | 2015-02-11 | 2015-02-11 | |
| US62/114,788 | 2015-02-11 | ||
| US201562150600P | 2015-04-21 | 2015-04-21 | |
| US62/150,600 | 2015-04-21 | ||
| PCT/US2016/017454 WO2016130746A1 (en) | 2015-02-11 | 2016-02-11 | Method and compositions for reducing non-specific amplification products |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2018506307A JP2018506307A (ja) | 2018-03-08 |
| JP2018506307A5 true JP2018506307A5 (cg-RX-API-DMAC7.html) | 2018-12-27 |
| JP6755890B2 JP6755890B2 (ja) | 2020-09-16 |
Family
ID=56566583
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017560886A Active JP6755890B2 (ja) | 2015-02-11 | 2016-02-11 | 非特異的増幅産物を減少させる為の方法及び組成物 |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US9464318B2 (cg-RX-API-DMAC7.html) |
| EP (1) | EP3256584B1 (cg-RX-API-DMAC7.html) |
| JP (1) | JP6755890B2 (cg-RX-API-DMAC7.html) |
| KR (1) | KR20170110721A (cg-RX-API-DMAC7.html) |
| CN (3) | CN107532166A (cg-RX-API-DMAC7.html) |
| AU (1) | AU2016219288B2 (cg-RX-API-DMAC7.html) |
| BR (1) | BR112017017142B1 (cg-RX-API-DMAC7.html) |
| CA (1) | CA2976251C (cg-RX-API-DMAC7.html) |
| ES (1) | ES2795404T3 (cg-RX-API-DMAC7.html) |
| WO (1) | WO2016130746A1 (cg-RX-API-DMAC7.html) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10421993B2 (en) | 2015-02-11 | 2019-09-24 | Paragon Genomics, Inc. | Methods and compositions for reducing non-specific amplification products |
| CN107058573B (zh) * | 2016-06-13 | 2020-07-10 | 艾吉泰康生物科技(北京)有限公司 | 一种利用Cas9/gRNA系统构建扩增子文库的方法 |
| CN110382716A (zh) * | 2017-01-10 | 2019-10-25 | 佰隆基因公司 | 用于减少引物延伸反应中产生的冗余分子条形码的方法和组合物 |
| EP3574109B1 (en) * | 2017-01-24 | 2023-07-26 | Vastogen, Inc. | Methods for constructing copies of nucleic acid molecules |
| CN111936635B (zh) * | 2018-03-02 | 2024-08-23 | 豪夫迈·罗氏有限公司 | 用于单分子测序的单链环状dna模板的产生 |
| US11976275B2 (en) | 2018-06-15 | 2024-05-07 | Kapa Biosystems, Inc. | Generation of double-stranded DNA templates for single molecule sequencing |
| JP7626430B2 (ja) * | 2019-01-09 | 2025-02-04 | 国立研究開発法人理化学研究所 | 薬物動態関連遺伝子の網羅的配列解析法とそれに使用されるプライマーセット |
| US11396674B2 (en) * | 2019-02-22 | 2022-07-26 | Gianluca ROMA | Multiplex nucleic acid amplification and library preparation |
| US10941453B1 (en) | 2020-05-20 | 2021-03-09 | Paragon Genomics, Inc. | High throughput detection of pathogen RNA in clinical specimens |
| CN114875119B (zh) * | 2021-02-05 | 2025-11-18 | 广东菲鹏生物有限公司 | 一种提高核酸扩增产物浓度的方法及其应用 |
| CN114369649A (zh) * | 2022-02-08 | 2022-04-19 | 山东见微生物科技有限公司 | 一种特异的选择扩增及多重pcr方法和应用 |
| US12091715B2 (en) | 2022-04-21 | 2024-09-17 | Paragon Genomics, Inc. | Methods and compositions for reducing base errors of massive parallel sequencing using triseq sequencing |
| US11680293B1 (en) | 2022-04-21 | 2023-06-20 | Paragon Genomics, Inc. | Methods and compositions for amplifying DNA and generating DNA sequencing results from target-enriched DNA molecules |
| CN114774526B (zh) * | 2022-04-25 | 2025-12-12 | 陕西科技大学 | 一种dna长度的熔解测定方法及试剂盒 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5750341A (en) | 1995-04-17 | 1998-05-12 | Lynx Therapeutics, Inc. | DNA sequencing by parallel oligonucleotide extensions |
| US20040002156A1 (en) * | 2002-06-26 | 2004-01-01 | Stratagene | Selective cloning of homoduplex nucleic acids |
| DE602004026033D1 (de) * | 2003-01-29 | 2010-04-29 | 454 Corp | Zweiendige sequenzierung |
| EP1574570A1 (en) * | 2004-03-12 | 2005-09-14 | Universität Regensburg | Process for reducing the number of mismatches in double stranded polynucleotides |
| DE602005024987D1 (de) * | 2004-08-24 | 2011-01-05 | Cornell Res Foundation Inc | Detektion von nucleinsäureunterschieden mit hilfe von endonucleasespaltungs-/ligaseabgabereaktionen und kapillarelektrophoresen oder mikroarrays |
| US20060281113A1 (en) | 2005-05-18 | 2006-12-14 | George Church | Accessible polynucleotide libraries and methods of use thereof |
| US8716190B2 (en) | 2007-09-14 | 2014-05-06 | Affymetrix, Inc. | Amplification and analysis of selected targets on solid supports |
| WO2012044847A1 (en) | 2010-10-01 | 2012-04-05 | Life Technologies Corporation | Nucleic acid adaptors and uses thereof |
| US8586310B2 (en) | 2008-09-05 | 2013-11-19 | Washington University | Method for multiplexed nucleic acid patch polymerase chain reaction |
| US9957558B2 (en) | 2011-04-28 | 2018-05-01 | Life Technologies Corporation | Methods and compositions for multiplex PCR |
| EP2943590A4 (en) | 2013-01-13 | 2017-02-15 | Unitaq Bio | Methods and compositions for pcr using blocked and universal primers |
| WO2015063154A1 (en) | 2013-10-31 | 2015-05-07 | Multiplicom N.V. | Blocking primers in multiplex pcr based assays |
-
2016
- 2016-02-11 CN CN201680021382.3A patent/CN107532166A/zh active Pending
- 2016-02-11 EP EP16749846.8A patent/EP3256584B1/en active Active
- 2016-02-11 WO PCT/US2016/017454 patent/WO2016130746A1/en not_active Ceased
- 2016-02-11 CN CN202510844827.9A patent/CN120700118A/zh active Pending
- 2016-02-11 CN CN202510844889.XA patent/CN120700119A/zh active Pending
- 2016-02-11 BR BR112017017142-2A patent/BR112017017142B1/pt active IP Right Grant
- 2016-02-11 US US15/041,644 patent/US9464318B2/en active Active
- 2016-02-11 AU AU2016219288A patent/AU2016219288B2/en active Active
- 2016-02-11 JP JP2017560886A patent/JP6755890B2/ja active Active
- 2016-02-11 CA CA2976251A patent/CA2976251C/en active Active
- 2016-02-11 ES ES16749846T patent/ES2795404T3/es active Active
- 2016-02-11 KR KR1020177025384A patent/KR20170110721A/ko not_active Ceased
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