CN108504651B - 基于高通量测序的pcr产物大样本量混合建库的文库构建方法和试剂 - Google Patents
基于高通量测序的pcr产物大样本量混合建库的文库构建方法和试剂 Download PDFInfo
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- CN108504651B CN108504651B CN201710107944.2A CN201710107944A CN108504651B CN 108504651 B CN108504651 B CN 108504651B CN 201710107944 A CN201710107944 A CN 201710107944A CN 108504651 B CN108504651 B CN 108504651B
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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Abstract
Description
名称 | 序列 | 名称 | 序列 |
标签01 | ACAGTG | 标签25 | CTATAC |
标签02 | ACTGAT | 标签26 | CTCAGA |
标签03 | ACTTGA | 标签27 | CTTGTA |
标签04 | AGTCAA | 标签28 | GACGAC |
标签05 | AGTTCC | 标签29 | GAGTGG |
标签06 | ATCACG | 标签30 | GATCAG |
标签07 | ATGAGC | 标签31 | GCCAAT |
标签08 | ATGTCA | 标签32 | GGCTAC |
标签09 | ATTCCT | 标签33 | GGTAGC |
标签10 | CAAAAG | 标签34 | GTAGAG |
标签11 | CAACTA | 标签35 | GTCCGC |
标签12 | CACCGG | 标签36 | GTGAAA |
标签13 | CACGAT | 标签37 | GTGGCC |
标签14 | CACTCA | 标签38 | GTTTCG |
标签15 | CAGATC | 标签39 | TAATCG |
标签16 | CAGGCG | 标签40 | TACAGC |
标签17 | CATGGC | 标签41 | TAGCTT |
标签18 | CATTTT | 标签42 | TATAAT |
标签19 | CCAACA | 标签43 | TCATTC |
标签20 | CCGTCC | 标签44 | TCCCGA |
标签21 | CGATGT | 标签45 | TCGAAG |
标签22 | CGGAAT | 标签46 | TCGGCA |
标签23 | CGTACG | 标签47 | TGACCA |
标签24 | CTAGCT | 标签48 | TTAGGC |
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CN108796054A (zh) * | 2018-09-14 | 2018-11-13 | 华大生物科技(武汉)有限公司 | 用于同时检测地中海贫血基因突变型与缺失型的试剂盒及其应用 |
CN109135762A (zh) * | 2018-10-30 | 2019-01-04 | 上海理工大学 | 一种用于促进蜜桔生长的微生物菌肥及其制备方法 |
CN113774121B (zh) * | 2021-09-13 | 2024-02-20 | 武汉大学 | 一种基于RNA连接标签的低样本量m6A高通量测序方法 |
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CN102796808B (zh) * | 2011-05-23 | 2014-06-18 | 深圳华大基因科技服务有限公司 | 甲基化高通量检测方法 |
CN104975081B (zh) * | 2015-06-01 | 2019-03-01 | 南京市妇幼保健院 | 检测pkd1基因突变的扩增引物、试剂盒及其检测方法 |
CN105200530A (zh) * | 2015-10-13 | 2015-12-30 | 北京百迈客生物科技有限公司 | 一种适用于高通量全基因组测序的多样品混合文库的构建方法 |
CN105297144A (zh) * | 2015-10-27 | 2016-02-03 | 北京百迈客生物科技有限公司 | 一种原核生物小rna的高通量文库构建方法 |
CN111621548A (zh) * | 2016-04-26 | 2020-09-04 | 序康医疗科技(苏州)有限公司 | 扩增dna的方法 |
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Effective date of registration: 20190220 Address after: 518000 Guangdong Province Dapeng New District Kwai Chung Street Life Science Industrial Park A11 Building 201 Applicant after: SHENZHEN LETU BIOLOGICAL TECHNOLOGY Co.,Ltd. Address before: 518000 Shenzhen Nanshan District, Shenzhen City, Guangdong Province, Shahexi Road, Shenzhen Bay Science and Technology Eco-Park, 2 C Blocks, 9 Floors Applicant before: SHENZHEN CHEERLAND PRECISION MEDICAL TECHNOLOGY CO.,LTD. |
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Denomination of invention: Library construction method and reagents for constructing a large sample size mixed library of PCR products based on high-throughput sequencing Effective date of registration: 20230925 Granted publication date: 20200908 Pledgee: Luohu sub branch of Shenzhen Rural Commercial Bank Co.,Ltd. Pledgor: SHENZHEN LETU BIOLOGICAL TECHNOLOGY Co.,Ltd. Registration number: Y2023980058624 |