JP2018505414A - サンプルバックグラウンドからの標的ポリヌクレオチドのナノ細孔検出方法 - Google Patents

サンプルバックグラウンドからの標的ポリヌクレオチドのナノ細孔検出方法 Download PDF

Info

Publication number
JP2018505414A
JP2018505414A JP2017540801A JP2017540801A JP2018505414A JP 2018505414 A JP2018505414 A JP 2018505414A JP 2017540801 A JP2017540801 A JP 2017540801A JP 2017540801 A JP2017540801 A JP 2017540801A JP 2018505414 A JP2018505414 A JP 2018505414A
Authority
JP
Japan
Prior art keywords
sample
target polynucleotide
amplification
nanopore
payload
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2017540801A
Other languages
English (en)
Japanese (ja)
Inventor
トレバー ジェイ. モリン
トレバー ジェイ. モリン
タイラー シュロップシャー
タイラー シュロップシャー
ウィリアム ビー. ダンバー
ウィリアム ビー. ダンバー
ダニエル エイ. ヘラー
ダニエル エイ. ヘラー
ホンユン ワン
ホンユン ワン
Original Assignee
ツー ポア ガイズ インコーポレイテッド
ツー ポア ガイズ インコーポレイテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ツー ポア ガイズ インコーポレイテッド, ツー ポア ガイズ インコーポレイテッド filed Critical ツー ポア ガイズ インコーポレイテッド
Publication of JP2018505414A publication Critical patent/JP2018505414A/ja
Pending legal-status Critical Current

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6848Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00Reactions of nucleic acids characterised by
    • C12Q2531/10Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113PCR
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/131Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a member of a cognate binding pair, i.e. extends to antibodies, haptens, avidin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60Detection means characterised by use of a special device
    • C12Q2565/631Detection means characterised by use of a special device being a biochannel or pore

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2017540801A 2015-02-02 2016-02-02 サンプルバックグラウンドからの標的ポリヌクレオチドのナノ細孔検出方法 Pending JP2018505414A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562111075P 2015-02-02 2015-02-02
US62/111,075 2015-02-02
PCT/US2016/016233 WO2016126746A1 (en) 2015-02-02 2016-02-02 Nanopore detection of target polynucleotides from sample background

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2018230605A Division JP2019092509A (ja) 2015-02-02 2018-12-10 サンプルバックグラウンドからの標的ポリヌクレオチドのナノ細孔検出方法

Publications (1)

Publication Number Publication Date
JP2018505414A true JP2018505414A (ja) 2018-02-22

Family

ID=56564617

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2017540801A Pending JP2018505414A (ja) 2015-02-02 2016-02-02 サンプルバックグラウンドからの標的ポリヌクレオチドのナノ細孔検出方法
JP2018230605A Pending JP2019092509A (ja) 2015-02-02 2018-12-10 サンプルバックグラウンドからの標的ポリヌクレオチドのナノ細孔検出方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2018230605A Pending JP2019092509A (ja) 2015-02-02 2018-12-10 サンプルバックグラウンドからの標的ポリヌクレオチドのナノ細孔検出方法

Country Status (12)

Country Link
US (1) US11098348B2 (https=)
EP (1) EP3253889A4 (https=)
JP (2) JP2018505414A (https=)
KR (2) KR20190127983A (https=)
CN (1) CN107250380A (https=)
AU (3) AU2016215453A1 (https=)
CA (1) CA2973753A1 (https=)
HK (1) HK1245348A1 (https=)
IL (1) IL253272B (https=)
MX (1) MX2017009766A (https=)
RU (1) RU2017127990A (https=)
WO (1) WO2016126746A1 (https=)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2020183933A (ja) * 2019-04-26 2020-11-12 ジ ユニバーシティ オブ オタワ 制御されたカウンティングを用いるナノポア捕獲率の校正
JP2021093991A (ja) * 2019-12-19 2021-06-24 株式会社日立製作所 プローブ、プローブセット、および溶液中の任意のdna配列を同定する方法
JP2023513128A (ja) * 2020-02-06 2023-03-30 エフ. ホフマン-ラ ロシュ アーゲー ナノポアへの鋳型の貫通を低減する組成物

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8870950B2 (en) 2009-12-08 2014-10-28 Mitral Tech Ltd. Rotation-based anchoring of an implant
US11653910B2 (en) 2010-07-21 2023-05-23 Cardiovalve Ltd. Helical anchor implantation
US20150351906A1 (en) 2013-01-24 2015-12-10 Mitraltech Ltd. Ventricularly-anchored prosthetic valves
WO2014182634A1 (en) 2013-05-06 2014-11-13 Two Pore Guys, Inc. A method of biological target detection using a nanopore and a fusion protein binding agent
KR102245192B1 (ko) 2013-05-06 2021-04-29 온테라 인크. 나노포어를 이용한 표적 검출
EP3174502B1 (en) 2014-07-30 2022-04-06 Cardiovalve Ltd Apparatus for implantation of an articulatable prosthetic valve
JP6702951B2 (ja) * 2014-09-26 2020-06-03 オンテラ インコーポレイテッド 合成プローブのナノ細孔センシングによる標的配列検出
EP3253333B1 (en) 2015-02-05 2024-04-03 Cardiovalve Ltd Prosthetic valve with axially-sliding frames
JP2018537673A (ja) * 2015-11-23 2018-12-20 ツー ポア ガイズ インコーポレイテッド 追跡及び検出のための標的修飾
US10531866B2 (en) 2016-02-16 2020-01-14 Cardiovalve Ltd. Techniques for providing a replacement valve and transseptal communication
US10640822B2 (en) 2016-02-29 2020-05-05 Iridia, Inc. Systems and methods for writing, reading, and controlling data stored in a polymer
US10438662B2 (en) 2016-02-29 2019-10-08 Iridia, Inc. Methods, compositions, and devices for information storage
US10859562B2 (en) 2016-02-29 2020-12-08 Iridia, Inc. Methods, compositions, and devices for information storage
US11486873B2 (en) 2016-03-31 2022-11-01 Ontera Inc. Multipore determination of fractional abundance of polynucleotide sequences in a sample
US20190231525A1 (en) 2016-08-01 2019-08-01 Mitraltech Ltd. Minimally-invasive delivery systems
ES3018641T3 (es) 2016-08-10 2025-05-16 Cardiovalve Ltd Válvula protésica con marcos concéntricos
AU2017348009B2 (en) 2016-10-24 2019-10-10 Ontera Inc. Fractional abundance of polynucleotide sequences in a sample
KR102076592B1 (ko) * 2017-05-25 2020-02-13 가천대학교 산학협력단 나노기공 센서를 사용한 dna 농도 측정 장치 및 방법
US11793633B2 (en) 2017-08-03 2023-10-24 Cardiovalve Ltd. Prosthetic heart valve
US10888421B2 (en) 2017-09-19 2021-01-12 Cardiovalve Ltd. Prosthetic heart valve with pouch
US12064347B2 (en) 2017-08-03 2024-08-20 Cardiovalve Ltd. Prosthetic heart valve
US12458493B2 (en) 2017-09-19 2025-11-04 Cardiovalve Ltd. Prosthetic heart valve and delivery systems and methods
CN108181358B (zh) * 2017-11-15 2020-02-11 华东理工大学 基于气单胞菌溶素纳米通道检测氨基酸的方法
CN109239140B (zh) * 2018-08-16 2021-04-13 广东第二师范学院 一种纳米孔功能性控制方法及系统
CN113966403A (zh) * 2019-04-22 2022-01-21 奥特拉公司 样品中多核苷酸序列的分数丰度的多孔测定
CN110438203B (zh) * 2019-08-19 2023-11-28 上海纳米技术及应用国家工程研究中心有限公司 一种提高环介导等温扩增反应效率的方法及应用
KR102358418B1 (ko) * 2019-12-31 2022-02-04 주식회사 제노헬릭스 스몰 rna 검출 방법
US11837302B1 (en) 2020-08-07 2023-12-05 Iridia, Inc. Systems and methods for writing and reading data stored in a polymer using nano-channels
US12357459B2 (en) 2020-12-03 2025-07-15 Cardiovalve Ltd. Transluminal delivery system

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05273209A (ja) * 1992-03-25 1993-10-22 Takara Shuzo Co Ltd Dna検出用キット
JPH11346798A (ja) * 1998-06-04 1999-12-21 Makoto Tsuruoka 核酸の測定方法およびVero毒素生産菌の検出方法
JP2013506418A (ja) * 2009-09-30 2013-02-28 クアンタポール, インコーポレイテッド 標識されたナノポアを使用する生物学的なポリマーの超高速配列決定
JP2014145771A (ja) * 2007-09-04 2014-08-14 Base4 Innovation Ltd 装置及び方法
US20140329225A1 (en) * 2013-05-06 2014-11-06 Two Pore Guys, Inc. Target detection with nanopore

Family Cites Families (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020197618A1 (en) 2001-01-20 2002-12-26 Sampson Jeffrey R. Synthesis and amplification of unstructured nucleic acids for rapid sequencing
AU2002359510A1 (en) * 2001-11-30 2003-06-17 Nanosphere, Inc. Real-time monitoring of pcr amplification using nanoparticle probes
US6952651B2 (en) 2002-06-17 2005-10-04 Intel Corporation Methods and apparatus for nucleic acid sequencing by signal stretching and data integration
WO2004001379A2 (en) 2002-06-20 2003-12-31 The Regents Of The University Of California Methods and compositions for detection and analysis of polynucleotides using light harvesting multichromophores
US7270956B2 (en) 2002-08-26 2007-09-18 The Regents Of The University Of California Methods and compositions for detection and analysis of polynucleotides using light harvesting multichromophores
US20040022764A1 (en) 2002-07-31 2004-02-05 Hanan Polansky Inhibition of microcompetition with a foreign polynucleotide as treatment of chronic disease
DE602004030114D1 (de) 2003-05-20 2010-12-30 Investigen Inc System zum nachweis von polynukleotiden
US20050186576A1 (en) 2004-02-19 2005-08-25 Intel Corporation Polymer sequencing using selectively labeled monomers and data integration
TWI291416B (en) * 2005-07-19 2007-12-21 Benq Corp Business equipment
KR100691806B1 (ko) * 2005-08-04 2007-03-12 삼성전자주식회사 비드 및 나노포어를 이용한 핵산 검출방법 및 검출장치
US20070048745A1 (en) 2005-08-30 2007-03-01 Joyce Timothy H Systems and methods for partitioned nanopore analysis of polymers
US20070190543A1 (en) * 2005-11-14 2007-08-16 Applera Corporation Coded Molecules for Detecting Target Analytes
WO2007117832A2 (en) * 2006-03-12 2007-10-18 Applera Corporation Methods of detecting target nucleic acids
US8889348B2 (en) 2006-06-07 2014-11-18 The Trustees Of Columbia University In The City Of New York DNA sequencing by nanopore using modified nucleotides
AU2008236694B2 (en) 2007-04-04 2014-01-23 The Regents Of The University Of California Compositions, devices, systems, and methods for using a nanopore
US20080268440A1 (en) * 2007-04-26 2008-10-30 Liu Timothy Z Biomolecule immobilization on surface via hydrophobic interactions
US8329647B2 (en) * 2008-07-17 2012-12-11 Ikfe Institut Fur Klinische Forschung Und Entwicklung Gmbh Method of treating a subject according to biomarkers for insulin resistance
US8262879B2 (en) 2008-09-03 2012-09-11 Nabsys, Inc. Devices and methods for determining the length of biopolymers and distances between probes bound thereto
KR101267789B1 (ko) 2010-06-25 2013-06-04 서울대학교산학협력단 나노 포어 구조를 이용한 dna 분석용 장치, 분석 방법 및 pcr 정량 검출 장치
WO2012088339A2 (en) 2010-12-22 2012-06-28 Genia Technologies, Inc. Nanopore-based single dna molecule characterization using speed bumps
WO2012135658A2 (en) 2011-03-30 2012-10-04 Noblegen Biosciences, Inc. Sequence preserved dna conversion for optical nanopore sequencing
US9926596B2 (en) * 2011-05-27 2018-03-27 Genapsys, Inc. Systems and methods for genetic and biological analysis
EP2734840B3 (en) 2011-07-20 2017-06-07 The Regents of the University of California Dual-pore device
AU2012288629B2 (en) 2011-07-25 2017-02-02 Oxford Nanopore Technologies Limited Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores
US20130040827A1 (en) * 2011-08-14 2013-02-14 Stephen C. Macevicz Method and compositions for detecting and sequencing nucleic acids
EP3674412A1 (en) * 2012-06-20 2020-07-01 The Trustees of Columbia University in the City of New York Nucleic acid sequencing by nanopore detection of tag molecules
WO2014024041A1 (en) 2012-08-06 2014-02-13 Quantumdx Group Limited Method and kit for nucleic acid sequencing
US20140099726A1 (en) 2012-10-10 2014-04-10 Two Pore Guys, Inc. Device for characterizing polymers
US9732379B2 (en) 2013-03-15 2017-08-15 The Curators Of The University Of Missouri Encoded nanopore sensor for multiplex nucleic acids detection
US20150011925A1 (en) * 2013-07-08 2015-01-08 Robert F. Buckman, Jr. Method and device for temporary emergency vessel anastomoses
CN107110817B (zh) * 2014-12-31 2019-09-24 卡尤迪生物科技(北京)有限公司 使用纳米孔和标签的核酸分子检测
CA2986921A1 (en) 2015-06-02 2016-12-08 Nanopore Diagnostics, Llc Nucleic acid detection
WO2018093976A1 (en) 2016-11-16 2018-05-24 Nanopore Diagnostics, Llc Analysis of nucleic acids using probe with non-linear tag

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05273209A (ja) * 1992-03-25 1993-10-22 Takara Shuzo Co Ltd Dna検出用キット
JPH11346798A (ja) * 1998-06-04 1999-12-21 Makoto Tsuruoka 核酸の測定方法およびVero毒素生産菌の検出方法
JP2014145771A (ja) * 2007-09-04 2014-08-14 Base4 Innovation Ltd 装置及び方法
JP2013506418A (ja) * 2009-09-30 2013-02-28 クアンタポール, インコーポレイテッド 標識されたナノポアを使用する生物学的なポリマーの超高速配列決定
US20140329225A1 (en) * 2013-05-06 2014-11-06 Two Pore Guys, Inc. Target detection with nanopore

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2020183933A (ja) * 2019-04-26 2020-11-12 ジ ユニバーシティ オブ オタワ 制御されたカウンティングを用いるナノポア捕獲率の校正
JP2021093991A (ja) * 2019-12-19 2021-06-24 株式会社日立製作所 プローブ、プローブセット、および溶液中の任意のdna配列を同定する方法
JP7344786B2 (ja) 2019-12-19 2023-09-14 株式会社日立製作所 溶液中の任意のdna配列を同定する方法
US12378597B2 (en) 2019-12-19 2025-08-05 Hitachi, Ltd. Probe, probe set, and method for identifying desired DNA sequence in solution
JP2023513128A (ja) * 2020-02-06 2023-03-30 エフ. ホフマン-ラ ロシュ アーゲー ナノポアへの鋳型の貫通を低減する組成物
JP7592731B2 (ja) 2020-02-06 2024-12-02 エフ. ホフマン-ラ ロシュ アーゲー ナノポアへの鋳型の貫通を低減する組成物

Also Published As

Publication number Publication date
WO2016126746A1 (en) 2016-08-11
EP3253889A4 (en) 2018-09-26
AU2016215453A1 (en) 2017-08-10
JP2019092509A (ja) 2019-06-20
MX2017009766A (es) 2017-12-11
AU2020239812A1 (en) 2020-10-29
RU2017127990A3 (https=) 2019-03-04
US20180023115A1 (en) 2018-01-25
RU2017127990A (ru) 2019-03-04
KR20170106487A (ko) 2017-09-20
HK1245348A1 (zh) 2018-08-24
US11098348B2 (en) 2021-08-24
KR20190127983A (ko) 2019-11-13
CA2973753A1 (en) 2016-08-11
IL253272B (en) 2018-08-30
AU2018208656A1 (en) 2018-08-09
EP3253889A1 (en) 2017-12-13
IL253272A0 (en) 2017-09-03
CN107250380A (zh) 2017-10-13

Similar Documents

Publication Publication Date Title
JP2018505414A (ja) サンプルバックグラウンドからの標的ポリヌクレオチドのナノ細孔検出方法
JP6541114B2 (ja) ナノポアによる標的検出法
KR20130130827A (ko) 이중-포어 디바이스
JP3844996B2 (ja) 反復性pcr産物の融解曲線解析方法
CN106471369B (zh) 利用纳米细孔的目标检测
JP7796858B2 (ja) ナノ細孔デバイス内で特徴をマッピングするための強制移動ポリヌクレオチドスキャンニング
JP6630672B2 (ja) Ngsシステム用の対照及びそれを用いる方法
Loh et al. Electric single-molecule hybridization detector for short DNA fragments
JP2021536261A (ja) ナノ細孔デバイスを使用して分子を検出するためのセンシング組成物、方法、およびデバイス
TWI539004B (zh) 擴增子長度的測量方法
EP4204585B1 (en) ULTRASENSITIVE QUANTIFICATION OF RNA USING NANOPORES
US11986823B2 (en) Microfluidic device and method for the nanostructure sequencing of nucleotide strands
US20200024650A1 (en) Target Polynucleotide Detection and Sequencing by Incorporation of Modified Nucleotides for Nanaopore Analysis
JP2021097703A (ja) 鋳型核酸の分析方法、標的物質の分析方法、鋳型核酸または標的物質の分析用キット、および鋳型核酸または標的物質の分析用装置
Sebuyoya Bioelectrochemistry in molecular oncology
HK1219511B (zh) 纳米流体中分子的表徵

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20170915

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20170915

A871 Explanation of circumstances concerning accelerated examination

Free format text: JAPANESE INTERMEDIATE CODE: A871

Effective date: 20170915

A975 Report on accelerated examination

Free format text: JAPANESE INTERMEDIATE CODE: A971005

Effective date: 20171129

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20171225

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20180308

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20180625

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20180809