MX2017009766A - Detección de nanoporo de polinucleótidos blanco de fondo de muestra. - Google Patents

Detección de nanoporo de polinucleótidos blanco de fondo de muestra.

Info

Publication number
MX2017009766A
MX2017009766A MX2017009766A MX2017009766A MX2017009766A MX 2017009766 A MX2017009766 A MX 2017009766A MX 2017009766 A MX2017009766 A MX 2017009766A MX 2017009766 A MX2017009766 A MX 2017009766A MX 2017009766 A MX2017009766 A MX 2017009766A
Authority
MX
Mexico
Prior art keywords
target polynucleotides
sample background
nanopore detection
detection
nanopore
Prior art date
Application number
MX2017009766A
Other languages
English (en)
Inventor
A Heller Daniel
J Morin Trevor
b dunbar William
Wang Hongyun
Shropshire Tyler
Original Assignee
Two Pore Guys Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Two Pore Guys Inc filed Critical Two Pore Guys Inc
Publication of MX2017009766A publication Critical patent/MX2017009766A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113—PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/131—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a member of a cognate binding pair, i.e. extends to antibodies, haptens, avidin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/631—Detection means characterised by use of a special device being a biochannel or pore

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Se describen en la presente métodos y composiciones para la detección de polinucleótidos blanco en muestra mezclada por amplificación del polinucleótido blanco y la detección en un dispositivo de nanoporo.
MX2017009766A 2015-02-02 2016-02-02 Detección de nanoporo de polinucleótidos blanco de fondo de muestra. MX2017009766A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562111075P 2015-02-02 2015-02-02
PCT/US2016/016233 WO2016126746A1 (en) 2015-02-02 2016-02-02 Nanopore detection of target polynucleotides from sample background

Publications (1)

Publication Number Publication Date
MX2017009766A true MX2017009766A (es) 2017-12-11

Family

ID=56564617

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2017009766A MX2017009766A (es) 2015-02-02 2016-02-02 Detección de nanoporo de polinucleótidos blanco de fondo de muestra.

Country Status (12)

Country Link
US (1) US11098348B2 (es)
EP (1) EP3253889A4 (es)
JP (2) JP2018505414A (es)
KR (2) KR20170106487A (es)
CN (1) CN107250380A (es)
AU (3) AU2016215453A1 (es)
CA (1) CA2973753A1 (es)
HK (1) HK1245348A1 (es)
IL (1) IL253272B (es)
MX (1) MX2017009766A (es)
RU (1) RU2017127990A (es)
WO (1) WO2016126746A1 (es)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8870950B2 (en) 2009-12-08 2014-10-28 Mitral Tech Ltd. Rotation-based anchoring of an implant
US11653910B2 (en) 2010-07-21 2023-05-23 Cardiovalve Ltd. Helical anchor implantation
EP2948103B1 (en) 2013-01-24 2022-12-07 Cardiovalve Ltd Ventricularly-anchored prosthetic valves
KR102245192B1 (ko) 2013-05-06 2021-04-29 온테라 인크. 나노포어를 이용한 표적 검출
ES2704902T3 (es) 2013-05-06 2019-03-20 Two Pore Guys Inc Un método de detección de objetivos biológicos usando un nanoporo y un agente de unión a proteínas de fusión
CN107205818B (zh) 2015-02-05 2019-05-10 卡迪尔维尔福股份有限公司 带有轴向滑动框架的人工瓣膜
MX2018006146A (es) * 2015-11-23 2019-05-27 Two Pore Guys Inc Modificación de diana para seguimiento y detección.
US10531866B2 (en) 2016-02-16 2020-01-14 Cardiovalve Ltd. Techniques for providing a replacement valve and transseptal communication
US10438662B2 (en) 2016-02-29 2019-10-08 Iridia, Inc. Methods, compositions, and devices for information storage
US10859562B2 (en) 2016-02-29 2020-12-08 Iridia, Inc. Methods, compositions, and devices for information storage
US10640822B2 (en) 2016-02-29 2020-05-05 Iridia, Inc. Systems and methods for writing, reading, and controlling data stored in a polymer
US11486873B2 (en) 2016-03-31 2022-11-01 Ontera Inc. Multipore determination of fractional abundance of polynucleotide sequences in a sample
CN114587712A (zh) 2016-08-10 2022-06-07 卡迪尔维尔福股份有限公司 具有同轴框架的人工瓣膜
KR102137178B1 (ko) 2016-10-24 2020-08-31 온테라 인크. 시료 내 폴리뉴클레오타이드 서열의 분포 분율
KR102076592B1 (ko) * 2017-05-25 2020-02-13 가천대학교 산학협력단 나노기공 센서를 사용한 dna 농도 측정 장치 및 방법
US10888421B2 (en) 2017-09-19 2021-01-12 Cardiovalve Ltd. Prosthetic heart valve with pouch
CN108181358B (zh) * 2017-11-15 2020-02-11 华东理工大学 基于气单胞菌溶素纳米通道检测氨基酸的方法
CN109239140B (zh) * 2018-08-16 2021-04-13 广东第二师范学院 一种纳米孔功能性控制方法及系统
JP2022530016A (ja) * 2019-04-22 2022-06-27 オンテラ インコーポレイテッド サンプル中のポリヌクレオチド配列の部分存在量の多孔決定
US20200340943A1 (en) * 2019-04-26 2020-10-29 The University Of Ottawa Calibrating Nanopore Capture Rates Using Controlled Counting
CN110438203B (zh) * 2019-08-19 2023-11-28 上海纳米技术及应用国家工程研究中心有限公司 一种提高环介导等温扩增反应效率的方法及应用
JP7344786B2 (ja) * 2019-12-19 2023-09-14 株式会社日立製作所 溶液中の任意のｄｎａ配列を同定する方法
KR102358418B1 (ko) * 2019-12-31 2022-02-04 주식회사 제노헬릭스 스몰 rna 검출 방법
EP4100415A1 (en) * 2020-02-06 2022-12-14 F. Hoffmann-La Roche AG Compositions that reduce template threading into a nanopore
US11837302B1 (en) 2020-08-07 2023-12-05 Iridia, Inc. Systems and methods for writing and reading data stored in a polymer using nano-channels

Family Cites Families (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05273209A (ja) 1992-03-25 1993-10-22 Takara Shuzo Co Ltd Ｄｎａ検出用キット
JPH11346798A (ja) 1998-06-04 1999-12-21 Makoto Tsuruoka 核酸の測定方法およびＶｅｒｏ毒素生産菌の検出方法
US20020197618A1 (en) * 2001-01-20 2002-12-26 Sampson Jeffrey R. Synthesis and amplification of unstructured nucleic acids for rapid sequencing
US20030143604A1 (en) * 2001-11-30 2003-07-31 Storhoff James J. Real-time monitoring of PCR amplification using nanoparticle probes
US6952651B2 (en) 2002-06-17 2005-10-04 Intel Corporation Methods and apparatus for nucleic acid sequencing by signal stretching and data integration
KR20050010956A (ko) * 2002-06-20 2005-01-28 더 리전츠 오브 더 유니버시티 오브 캘리포니아 집광 다발색단을 사용하여 폴리뉴클레오티드를 검출 및분석하기 위한 방법 및 조성물
US20040022764A1 (en) * 2002-07-31 2004-02-05 Hanan Polansky Inhibition of microcompetition with a foreign polynucleotide as treatment of chronic disease
WO2004092324A2 (en) 2002-08-26 2004-10-28 The Regents Of The University Of California Methods and compositions for detection and analysis of polynucleotides using light harvesting multichromophores
CN1894423A (zh) 2003-05-20 2007-01-10 因维斯蒂恩有限公司 检测多核苷酸的系统
US20050186576A1 (en) 2004-02-19 2005-08-25 Intel Corporation Polymer sequencing using selectively labeled monomers and data integration
TWI291416B (en) * 2005-07-19 2007-12-21 Benq Corp Business equipment
KR100691806B1 (ko) 2005-08-04 2007-03-12 삼성전자주식회사 비드 및 나노포어를 이용한 핵산 검출방법 및 검출장치
US20070048745A1 (en) * 2005-08-30 2007-03-01 Joyce Timothy H Systems and methods for partitioned nanopore analysis of polymers
EP1954838B1 (en) * 2005-11-14 2014-02-26 Life Technologies Corporation Coded molecules for detecting target analytes
US20100291548A1 (en) * 2006-03-12 2010-11-18 Applera Corporation Methods of Detecting Target Nucleic Acids
US8889348B2 (en) 2006-06-07 2014-11-18 The Trustees Of Columbia University In The City Of New York DNA sequencing by nanopore using modified nucleotides
EP3798317B1 (en) 2007-04-04 2024-01-03 The Regents of the University of California Compositions, devices, systems, and methods for using a nanopore
US20080268440A1 (en) * 2007-04-26 2008-10-30 Liu Timothy Z Biomolecule immobilization on surface via hydrophobic interactions
GB0717150D0 (en) 2007-09-04 2007-10-17 Univ Warwick Apparatus and method
EP2321647B1 (en) * 2008-07-17 2013-09-04 IKFE Institut für klinische Forschung und Entwicklung GmbH Biomarkers for insulin resistance and beta-cell dysfunction
US8262879B2 (en) 2008-09-03 2012-09-11 Nabsys, Inc. Devices and methods for determining the length of biopolymers and distances between probes bound thereto
JP2013506418A (ja) 2009-09-30 2013-02-28 クアンタポール， インコーポレイテッド 標識されたナノポアを使用する生物学的なポリマーの超高速配列決定
KR101267789B1 (ko) 2010-06-25 2013-06-04 서울대학교산학협력단 나노 포어 구조를 이용한 ｄｎａ 분석용 장치, 분석 방법 및 ｐｃｒ 정량 검출 장치
WO2012088339A2 (en) 2010-12-22 2012-06-28 Genia Technologies, Inc. Nanopore-based single dna molecule characterization using speed bumps
WO2012135658A2 (en) 2011-03-30 2012-10-04 Noblegen Biosciences, Inc. Sequence preserved dna conversion for optical nanopore sequencing
US9926596B2 (en) * 2011-05-27 2018-03-27 Genapsys, Inc. Systems and methods for genetic and biological analysis
KR101820834B1 (ko) 2011-07-20 2018-01-22 더 리전트 오브 더 유니버시티 오브 캘리포니아 이중-포어 디바이스
JP6298404B2 (ja) 2011-07-25 2018-03-20 オックスフォード ナノポール テクノロジーズ リミテッド 膜貫通ポアを用いる二重鎖ポリヌクレオチド配列決定のためのヘアピンループ方法
US20130040827A1 (en) * 2011-08-14 2013-02-14 Stephen C. Macevicz Method and compositions for detecting and sequencing nucleic acids
EP2864502B1 (en) * 2012-06-20 2019-10-23 The Trustees of Columbia University in the City of New York Nucleic acid sequencing by nanopore detection of tag molecules
WO2014024041A1 (en) 2012-08-06 2014-02-13 Quantumdx Group Limited Method and kit for nucleic acid sequencing
US20140099726A1 (en) 2012-10-10 2014-04-10 Two Pore Guys, Inc. Device for characterizing polymers
JP2016517275A (ja) 2013-03-15 2016-06-16 ザ キュレイターズ オブ ザ ユニバーシティ オブ ミズーリ 多重核酸検出のためのコード化ナノポアセンサー
ES2704902T3 (es) 2013-05-06 2019-03-20 Two Pore Guys Inc Un método de detección de objetivos biológicos usando un nanoporo y un agente de unión a proteínas de fusión
US20150011925A1 (en) * 2013-07-08 2015-01-08 Robert F. Buckman, Jr. Method and device for temporary emergency vessel anastomoses
CN107110817B (zh) * 2014-12-31 2019-09-24 卡尤迪生物科技（北京）有限公司 使用纳米孔和标签的核酸分子检测
CA2986921A1 (en) 2015-06-02 2016-12-08 Nanopore Diagnostics, Llc Nucleic acid detection
WO2018093976A1 (en) 2016-11-16 2018-05-24 Nanopore Diagnostics, Llc Analysis of nucleic acids using probe with non-linear tag

Also Published As

Publication number Publication date
KR20190127983A (ko) 2019-11-13
RU2017127990A (ru) 2019-03-04
KR20170106487A (ko) 2017-09-20
AU2016215453A1 (en) 2017-08-10
AU2020239812A1 (en) 2020-10-29
EP3253889A4 (en) 2018-09-26
JP2018505414A (ja) 2018-02-22
CA2973753A1 (en) 2016-08-11
JP2019092509A (ja) 2019-06-20
IL253272A0 (en) 2017-09-03
IL253272B (en) 2018-08-30
US11098348B2 (en) 2021-08-24
EP3253889A1 (en) 2017-12-13
RU2017127990A3 (es) 2019-03-04
US20180023115A1 (en) 2018-01-25
WO2016126746A1 (en) 2016-08-11
HK1245348A1 (zh) 2018-08-24
AU2018208656A1 (en) 2018-08-09
CN107250380A (zh) 2017-10-13

Similar Documents

Publication Publication Date Title
MX2017009766A (es) Detección de nanoporo de polinucleótidos blanco de fondo de muestra.
IL265396A (en) Methods and compositions for detecting analytes
MX2017003790A (es) Detección de secuencias diana por percepción de nanoporos de sondas sintéticas.
IL249618A0 (en) Methods and preparations for analyzing samples
EP3389481A4 (en) PROCESSES, COMPOSITIONS, KITS AND DEVICES FOR FAST LANALYSIS OF BIOLOGICAL MARKERS
SG10202108763UA (en) Methods and compositions for nucleic acid analysis
EP3158085A4 (en) Methods, compositions, and devices for rapid analysis of biological markers
EP3156498A4 (en) Breast cancer detection kit or device, and method for detecting breast cancer
PL3110975T3 (pl) Metody analizy somatycznych elementów ruchomych i ich zastosowania
EP3420083A4 (en) METHOD AND COMPOSITIONS FOR TARGET RECOGNITION
EP3159398A4 (en) Liver cancer detection kit or device, and detection method
MX2017001405A (es) Deteccion de acidos nucleicos diana mediante el uso de hibridacion.
MX2021006891A (es) Prueba de resistencia a patogenos y antimicrobiana.
EP3382396A4 (en) SAMPLE ANALYSIS DEVICE
EP3232203A4 (en) Substrate for sample analysis, sample analysis device, sample analysis system, and program for sample analysis system
GB2550006B (en) Establishing presence by identifying audio sample and position
EP3203216A4 (en) Luminescence measuring device and automatic analysis device
MX2017005888A (es) Dispositivos, sistemas y metodos para la deteccion de analitos.
EP3399319A4 (en) Specimen analysis substrate, specimen analysis device, specimen analysis system, and program for specimen analysis system
EP3163306A4 (en) Substrate for sample analysis, and sample analysis apparatus
EP3108045A4 (en) Compositions and methods for detecting microorganisms
WO2016153880A3 (en) Methods for detection of rnase activity
ZA201607571B (en) Compositions and methods for detecting huanglongbing
EP3264099A4 (en) Automatic analysis apparatus
AP2017009731A0 (en) Compositions and methods for the detection and analysis of mycobacterium tuberculosis