KR20170106487A - 시료 배경으로부터의 표적 폴리뉴클레오티드의 나노포어 검출 - Google Patents

시료 배경으로부터의 표적 폴리뉴클레오티드의 나노포어 검출 Download PDF

Info

Publication number
KR20170106487A
KR20170106487A KR1020177024505A KR20177024505A KR20170106487A KR 20170106487 A KR20170106487 A KR 20170106487A KR 1020177024505 A KR1020177024505 A KR 1020177024505A KR 20177024505 A KR20177024505 A KR 20177024505A KR 20170106487 A KR20170106487 A KR 20170106487A
Authority
KR
South Korea
Prior art keywords
target polynucleotide
sample
nanopore
amplification
payload
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
KR1020177024505A
Other languages
English (en)
Korean (ko)
Inventor
트레버 제이. 모린
타일러 슈롭셔
윌리엄 비. 던바
대니얼 에이. 헬러
홍연 왕
Original Assignee
투 포어 가이즈, 인코포레이티드
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 투 포어 가이즈, 인코포레이티드 filed Critical 투 포어 가이즈, 인코포레이티드
Publication of KR20170106487A publication Critical patent/KR20170106487A/ko
Ceased legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113—PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/131—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a member of a cognate binding pair, i.e. extends to antibodies, haptens, avidin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/631—Detection means characterised by use of a special device being a biochannel or pore

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
KR1020177024505A 2015-02-02 2016-02-02 시료 배경으로부터의 표적 폴리뉴클레오티드의 나노포어 검출 Ceased KR20170106487A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562111075P 2015-02-02 2015-02-02
US62/111,075 2015-02-02
PCT/US2016/016233 WO2016126746A1 (en) 2015-02-02 2016-02-02 Nanopore detection of target polynucleotides from sample background

Related Child Applications (1)

Application Number Title Priority Date Filing Date
KR1020197032635A Division KR20190127983A (ko) 2015-02-02 2016-02-02 시료 배경으로부터의 표적 폴리뉴클레오티드의 나노포어 검출

Publications (1)

Publication Number Publication Date
KR20170106487A true KR20170106487A (ko) 2017-09-20

Family

ID=56564617

Family Applications (2)

Application Number Title Priority Date Filing Date
KR1020177024505A Ceased KR20170106487A (ko) 2015-02-02 2016-02-02 시료 배경으로부터의 표적 폴리뉴클레오티드의 나노포어 검출
KR1020197032635A Withdrawn KR20190127983A (ko) 2015-02-02 2016-02-02 시료 배경으로부터의 표적 폴리뉴클레오티드의 나노포어 검출

Family Applications After (1)

Application Number Title Priority Date Filing Date
KR1020197032635A Withdrawn KR20190127983A (ko) 2015-02-02 2016-02-02 시료 배경으로부터의 표적 폴리뉴클레오티드의 나노포어 검출

Country Status (12)

Country Link
US (1) US11098348B2 (https=)
EP (1) EP3253889A4 (https=)
JP (2) JP2018505414A (https=)
KR (2) KR20170106487A (https=)
CN (1) CN107250380A (https=)
AU (3) AU2016215453A1 (https=)
CA (1) CA2973753A1 (https=)
HK (1) HK1245348A1 (https=)
IL (1) IL253272B (https=)
MX (1) MX2017009766A (https=)
RU (1) RU2017127990A (https=)
WO (1) WO2016126746A1 (https=)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8870950B2 (en) 2009-12-08 2014-10-28 Mitral Tech Ltd. Rotation-based anchoring of an implant
US11653910B2 (en) 2010-07-21 2023-05-23 Cardiovalve Ltd. Helical anchor implantation
EP2948103B1 (en) 2013-01-24 2022-12-07 Cardiovalve Ltd Ventricularly-anchored prosthetic valves
ES2704902T3 (es) 2013-05-06 2019-03-20 Two Pore Guys Inc Un método de detección de objetivos biológicos usando un nanoporo y un agente de unión a proteínas de fusión
KR102245192B1 (ko) 2013-05-06 2021-04-29 온테라 인크. 나노포어를 이용한 표적 검출
EP3174502B1 (en) 2014-07-30 2022-04-06 Cardiovalve Ltd Apparatus for implantation of an articulatable prosthetic valve
KR20170064540A (ko) * 2014-09-26 2017-06-09 투 포어 가이즈, 인코포레이티드 합성 프로브의 나노포어 감지에 의한 표적 서열 검출
EP3253333B1 (en) 2015-02-05 2024-04-03 Cardiovalve Ltd Prosthetic valve with axially-sliding frames
JP2018537673A (ja) * 2015-11-23 2018-12-20 ツー ポア ガイズ インコーポレイテッド 追跡及び検出のための標的修飾
US10531866B2 (en) 2016-02-16 2020-01-14 Cardiovalve Ltd. Techniques for providing a replacement valve and transseptal communication
US10438662B2 (en) * 2016-02-29 2019-10-08 Iridia, Inc. Methods, compositions, and devices for information storage
US10640822B2 (en) 2016-02-29 2020-05-05 Iridia, Inc. Systems and methods for writing, reading, and controlling data stored in a polymer
US10859562B2 (en) 2016-02-29 2020-12-08 Iridia, Inc. Methods, compositions, and devices for information storage
US11486873B2 (en) 2016-03-31 2022-11-01 Ontera Inc. Multipore determination of fractional abundance of polynucleotide sequences in a sample
WO2018081178A1 (en) * 2016-10-24 2018-05-03 Two Pore Guys, Inc. Fractional abundance of polynucleotide sequences in a sample
US20190231525A1 (en) 2016-08-01 2019-08-01 Mitraltech Ltd. Minimally-invasive delivery systems
CN109789018B (zh) 2016-08-10 2022-04-26 卡迪尔维尔福股份有限公司 具有同轴框架的人工瓣膜
KR102076592B1 (ko) * 2017-05-25 2020-02-13 가천대학교 산학협력단 나노기공 센서를 사용한 dna 농도 측정 장치 및 방법
US12064347B2 (en) 2017-08-03 2024-08-20 Cardiovalve Ltd. Prosthetic heart valve
US10888421B2 (en) 2017-09-19 2021-01-12 Cardiovalve Ltd. Prosthetic heart valve with pouch
US11793633B2 (en) 2017-08-03 2023-10-24 Cardiovalve Ltd. Prosthetic heart valve
US12458493B2 (en) 2017-09-19 2025-11-04 Cardiovalve Ltd. Prosthetic heart valve and delivery systems and methods
CN108181358B (zh) * 2017-11-15 2020-02-11 华东理工大学 基于气单胞菌溶素纳米通道检测氨基酸的方法
CN109239140B (zh) * 2018-08-16 2021-04-13 广东第二师范学院 一种纳米孔功能性控制方法及系统
CN113966403A (zh) * 2019-04-22 2022-01-21 奥特拉公司 样品中多核苷酸序列的分数丰度的多孔测定
US20200340943A1 (en) * 2019-04-26 2020-10-29 The University Of Ottawa Calibrating Nanopore Capture Rates Using Controlled Counting
CN110438203B (zh) * 2019-08-19 2023-11-28 上海纳米技术及应用国家工程研究中心有限公司 一种提高环介导等温扩增反应效率的方法及应用
JP7344786B2 (ja) 2019-12-19 2023-09-14 株式会社日立製作所 溶液中の任意のｄｎａ配列を同定する方法
KR102358418B1 (ko) * 2019-12-31 2022-02-04 주식회사 제노헬릭스 스몰 rna 검출 방법
CN115052882B (zh) * 2020-02-06 2024-05-24 豪夫迈·罗氏有限公司 减少模板穿入纳米孔的组合物
US11837302B1 (en) 2020-08-07 2023-12-05 Iridia, Inc. Systems and methods for writing and reading data stored in a polymer using nano-channels
US12357459B2 (en) 2020-12-03 2025-07-15 Cardiovalve Ltd. Transluminal delivery system

Family Cites Families (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05273209A (ja) 1992-03-25 1993-10-22 Takara Shuzo Co Ltd Ｄｎａ検出用キット
JPH11346798A (ja) 1998-06-04 1999-12-21 Makoto Tsuruoka 核酸の測定方法およびＶｅｒｏ毒素生産菌の検出方法
US20020197618A1 (en) * 2001-01-20 2002-12-26 Sampson Jeffrey R. Synthesis and amplification of unstructured nucleic acids for rapid sequencing
WO2003048769A1 (en) * 2001-11-30 2003-06-12 Nanosphere, Inc. Real-time monitoring of pcr amplification using nanoparticle probes
US6952651B2 (en) 2002-06-17 2005-10-04 Intel Corporation Methods and apparatus for nucleic acid sequencing by signal stretching and data integration
CN1675377B (zh) * 2002-06-20 2012-10-03 加州大学评议会 利用获取光的多发色团检测和分析多核苷酸的方法和组合物
US20040022764A1 (en) * 2002-07-31 2004-02-05 Hanan Polansky Inhibition of microcompetition with a foreign polynucleotide as treatment of chronic disease
KR20050055717A (ko) * 2002-08-26 2005-06-13 더 리전츠 오브 더 유니버시티 오브 캘리포니아 집광 다발색단을 사용하여 폴리뉴클레오티드를 검출 및 분석하기 위한 방법 및 조성물
ATE488604T1 (de) 2003-05-20 2010-12-15 Investigen Inc System zum nachweis von polynukleotiden
US20050186576A1 (en) 2004-02-19 2005-08-25 Intel Corporation Polymer sequencing using selectively labeled monomers and data integration
TWI291416B (en) * 2005-07-19 2007-12-21 Benq Corp Business equipment
KR100691806B1 (ko) 2005-08-04 2007-03-12 삼성전자주식회사 비드 및 나노포어를 이용한 핵산 검출방법 및 검출장치
US20070048745A1 (en) * 2005-08-30 2007-03-01 Joyce Timothy H Systems and methods for partitioned nanopore analysis of polymers
WO2007120265A2 (en) * 2005-11-14 2007-10-25 Applera Corporation Coded molecules for detecting target analytes
US20100291548A1 (en) * 2006-03-12 2010-11-18 Applera Corporation Methods of Detecting Target Nucleic Acids
WO2007146158A1 (en) 2006-06-07 2007-12-21 The Trustees Of Columbia University In The City Of New York Dna sequencing by nanopore using modified nucleotides
EP2156179B1 (en) 2007-04-04 2021-08-18 The Regents of The University of California Methods for using a nanopore
US20080268440A1 (en) * 2007-04-26 2008-10-30 Liu Timothy Z Biomolecule immobilization on surface via hydrophobic interactions
GB0717150D0 (en) 2007-09-04 2007-10-17 Univ Warwick Apparatus and method
EP2321647B1 (en) * 2008-07-17 2013-09-04 IKFE Institut für klinische Forschung und Entwicklung GmbH Biomarkers for insulin resistance and beta-cell dysfunction
US8262879B2 (en) 2008-09-03 2012-09-11 Nabsys, Inc. Devices and methods for determining the length of biopolymers and distances between probes bound thereto
EP2483680A4 (en) 2009-09-30 2014-01-01 Quantapore Inc ULTRASOUND SEQUENCING OF BIOLOGICAL POLYMERS WITH THE HELP OF A MARKED NANOPORE
KR101267789B1 (ko) 2010-06-25 2013-06-04 서울대학교산학협력단 나노 포어 구조를 이용한 ｄｎａ 분석용 장치, 분석 방법 및 ｐｃｒ 정량 검출 장치
GB2500360B (en) 2010-12-22 2019-10-23 Genia Tech Inc Nanopore-based single DNA molecule characterization, identification and isolation using speed bumps
US20120316075A1 (en) 2011-03-30 2012-12-13 Noblegen Biosciences, Inc. Sequence preserved dna conversion for optical nanopore sequencing
US9926596B2 (en) * 2011-05-27 2018-03-27 Genapsys, Inc. Systems and methods for genetic and biological analysis
EP3318872A1 (en) 2011-07-20 2018-05-09 The Regents of the University of California Dual-pore device
JP6298404B2 (ja) * 2011-07-25 2018-03-20 オックスフォード ナノポール テクノロジーズ リミテッド 膜貫通ポアを用いる二重鎖ポリヌクレオチド配列決定のためのヘアピンループ方法
US20130040827A1 (en) * 2011-08-14 2013-02-14 Stephen C. Macevicz Method and compositions for detecting and sequencing nucleic acids
EP3674412A1 (en) * 2012-06-20 2020-07-01 The Trustees of Columbia University in the City of New York Nucleic acid sequencing by nanopore detection of tag molecules
JP2015528282A (ja) 2012-08-06 2015-09-28 クワンタムディーエックス・グループ・リミテッド 核酸シークエンシングのための方法およびキット
US20140099726A1 (en) 2012-10-10 2014-04-10 Two Pore Guys, Inc. Device for characterizing polymers
AU2014227754B2 (en) 2013-03-15 2018-03-01 The Curators Of The University Of Missouri Encoded nanopore sensor for multiplex nucleic acids detection
ES2704902T3 (es) 2013-05-06 2019-03-20 Two Pore Guys Inc Un método de detección de objetivos biológicos usando un nanoporo y un agente de unión a proteínas de fusión
US20150011925A1 (en) * 2013-07-08 2015-01-08 Robert F. Buckman, Jr. Method and device for temporary emergency vessel anastomoses
CN107110817B (zh) * 2014-12-31 2019-09-24 卡尤迪生物科技（北京）有限公司 使用纳米孔和标签的核酸分子检测
WO2016196625A1 (en) 2015-06-02 2016-12-08 Nanopore Diagnostics, Llc Nucleic acid detection
WO2018093976A1 (en) 2016-11-16 2018-05-24 Nanopore Diagnostics, Llc Analysis of nucleic acids using probe with non-linear tag

Also Published As

Publication number Publication date
HK1245348A1 (zh) 2018-08-24
WO2016126746A1 (en) 2016-08-11
EP3253889A1 (en) 2017-12-13
RU2017127990A (ru) 2019-03-04
MX2017009766A (es) 2017-12-11
RU2017127990A3 (https=) 2019-03-04
JP2018505414A (ja) 2018-02-22
US11098348B2 (en) 2021-08-24
AU2020239812A1 (en) 2020-10-29
AU2016215453A1 (en) 2017-08-10
AU2018208656A1 (en) 2018-08-09
IL253272A0 (en) 2017-09-03
US20180023115A1 (en) 2018-01-25
JP2019092509A (ja) 2019-06-20
EP3253889A4 (en) 2018-09-26
IL253272B (en) 2018-08-30
CN107250380A (zh) 2017-10-13
CA2973753A1 (en) 2016-08-11
KR20190127983A (ko) 2019-11-13

Similar Documents

Publication Publication Date Title
KR20170106487A (ko) 시료 배경으로부터의 표적 폴리뉴클레오티드의 나노포어 검출
US12584883B2 (en) Nanopore functionality control
JP6541114B2 (ja) ナノポアによる標的検出法
KR101661297B1 (ko) 이중-포어 디바이스
Timp et al. Nanopore sequencing: electrical measurements of the code of life
US20120074925A1 (en) Assay Methods Using Nicking Endonucleases
JP2015534063A (ja) Ｄｎａおよびｒｎａ上のタンパク質およびその他修飾の解析のための方法およびシステム
JP6630672B2 (ja) Ｎｇｓシステム用の対照及びそれを用いる方法
Vu et al. Employing LiCl salt gradient in the wild-type α-hemolysin nanopore to slow down DNA translocation and detect methylated cytosine
Loh et al. Electric single-molecule hybridization detector for short DNA fragments
Zhao et al. Translocation of tetrahedral DNA nanostructures through a solid-state nanopore
JP2021536261A (ja) ナノ細孔デバイスを使用して分子を検出するためのセンシング組成物、方法、およびデバイス
US20230194500A1 (en) Ultrasensitive rna quantification using nanopores
WO2024173858A1 (en) Methods and devices for using enzymatic amplification and fragmentation to detect biomarkers with nanopores
AU2015259187A1 (en) Selective analysis of modified biological molecules with solid-state nanopores
HK1234825A1 (en) Target detection with nanopore

Legal Events

Date Code Title Description
A201 Request for examination
A302 Request for accelerated examination
AMND Amendment
PA0105 International application

Patent event date: 20170831

Patent event code: PA01051R01D

Comment text: International Patent Application

PA0201 Request for examination

Patent event code: PA02012R01D

Patent event date: 20170831

Comment text: Request for Examination of Application

PA0302 Request for accelerated examination

Patent event date: 20170831

Patent event code: PA03022R01D

Comment text: Request for Accelerated Examination

PG1501 Laying open of application
E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20180116

Patent event code: PE09021S01D

AMND Amendment
E601 Decision to refuse application
PE0601 Decision on rejection of patent

Patent event date: 20180927

Comment text: Decision to Refuse Application

Patent event code: PE06012S01D

Patent event date: 20180116

Comment text: Notification of reason for refusal

Patent event code: PE06011S01I

X091 Application refused [patent]
AMND Amendment
PX0901 Re-examination

Patent event code: PX09011S01I

Patent event date: 20180927

Comment text: Decision to Refuse Application

Patent event code: PX09012R01I

Patent event date: 20180716

Comment text: Amendment to Specification, etc.

Patent event code: PX09012R01I

Patent event date: 20170831

Comment text: Amendment to Specification, etc.

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20190124

Patent event code: PE09021S01D

AMND Amendment
PX0601 Decision of rejection after re-examination

Comment text: Decision to Refuse Application

Patent event code: PX06014S01D

Patent event date: 20190926

Comment text: Amendment to Specification, etc.

Patent event code: PX06012R01I

Patent event date: 20190715

Comment text: Notification of reason for refusal

Patent event code: PX06013S01I

Patent event date: 20190124

Comment text: Amendment to Specification, etc.

Patent event code: PX06012R01I

Patent event date: 20181221

Comment text: Decision to Refuse Application

Patent event code: PX06011S01I

Patent event date: 20180927

Comment text: Amendment to Specification, etc.

Patent event code: PX06012R01I

Patent event date: 20180716

Comment text: Notification of reason for refusal

Patent event code: PX06013S01I

Patent event date: 20180116

Comment text: Amendment to Specification, etc.

Patent event code: PX06012R01I

Patent event date: 20170831

X601 Decision of rejection after re-examination
A107 Divisional application of patent
PA0104 Divisional application for international application

Comment text: Divisional Application for International Patent

Patent event code: PA01041R01D

Patent event date: 20191104