CN107250380A - 来自样品背景的靶多核苷酸的纳米孔隙检测 - Google Patents

来自样品背景的靶多核苷酸的纳米孔隙检测 Download PDF

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Publication number
CN107250380A
CN107250380A CN201680008155.7A CN201680008155A CN107250380A CN 107250380 A CN107250380 A CN 107250380A CN 201680008155 A CN201680008155 A CN 201680008155A CN 107250380 A CN107250380 A CN 107250380A
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target polynucleotide
sample
nanoaperture
amplification
amplicon
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Chinese (zh)
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T·J·莫林
T·施罗普施雷
W·B·邓巴
D·A·海勒
H·王
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Double Man Co
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Double Man Co
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113—PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/131—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a member of a cognate binding pair, i.e. extends to antibodies, haptens, avidin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/631—Detection means characterised by use of a special device being a biochannel or pore

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  • Chemical & Material Sciences (AREA)
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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN201680008155.7A 2015-02-02 2016-02-02 来自样品背景的靶多核苷酸的纳米孔隙检测 Pending CN107250380A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562111075P 2015-02-02 2015-02-02
US62/111,075 2015-02-02
PCT/US2016/016233 WO2016126746A1 (en) 2015-02-02 2016-02-02 Nanopore detection of target polynucleotides from sample background

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CN107250380A true CN107250380A (zh) 2017-10-13

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US (1) US11098348B2 (https=)
EP (1) EP3253889A4 (https=)
JP (2) JP2018505414A (https=)
KR (2) KR20190127983A (https=)
CN (1) CN107250380A (https=)
AU (3) AU2016215453A1 (https=)
CA (1) CA2973753A1 (https=)
HK (1) HK1245348A1 (https=)
IL (1) IL253272B (https=)
MX (1) MX2017009766A (https=)
RU (1) RU2017127990A (https=)
WO (1) WO2016126746A1 (https=)

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CN108181358A (zh) * 2017-11-15 2018-06-19 华东理工大学 基于气单胞菌溶素纳米通道检测氨基酸的方法
CN109239140A (zh) * 2018-08-16 2019-01-18 广东第二师范学院 一种纳米孔功能性控制方法及系统
CN110438203A (zh) * 2019-08-19 2019-11-12 上海纳米技术及应用国家工程研究中心有限公司 一种提高环介导等温扩增反应效率的方法及应用
CN113966403A (zh) * 2019-04-22 2022-01-21 奥特拉公司 样品中多核苷酸序列的分数丰度的多孔测定

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US10531866B2 (en) 2016-02-16 2020-01-14 Cardiovalve Ltd. Techniques for providing a replacement valve and transseptal communication
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US20190231525A1 (en) 2016-08-01 2019-08-01 Mitraltech Ltd. Minimally-invasive delivery systems
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KR102076592B1 (ko) * 2017-05-25 2020-02-13 가천대학교 산학협력단 나노기공 센서를 사용한 dna 농도 측정 장치 및 방법
US11793633B2 (en) 2017-08-03 2023-10-24 Cardiovalve Ltd. Prosthetic heart valve
US10888421B2 (en) 2017-09-19 2021-01-12 Cardiovalve Ltd. Prosthetic heart valve with pouch
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US12458493B2 (en) 2017-09-19 2025-11-04 Cardiovalve Ltd. Prosthetic heart valve and delivery systems and methods
US20200340943A1 (en) * 2019-04-26 2020-10-29 The University Of Ottawa Calibrating Nanopore Capture Rates Using Controlled Counting
JP7344786B2 (ja) * 2019-12-19 2023-09-14 株式会社日立製作所 溶液中の任意のｄｎａ配列を同定する方法
KR102358418B1 (ko) * 2019-12-31 2022-02-04 주식회사 제노헬릭스 스몰 rna 검출 방법
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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108181358A (zh) * 2017-11-15 2018-06-19 华东理工大学 基于气单胞菌溶素纳米通道检测氨基酸的方法
CN108181358B (zh) * 2017-11-15 2020-02-11 华东理工大学 基于气单胞菌溶素纳米通道检测氨基酸的方法
CN109239140A (zh) * 2018-08-16 2019-01-18 广东第二师范学院 一种纳米孔功能性控制方法及系统
CN109239140B (zh) * 2018-08-16 2021-04-13 广东第二师范学院 一种纳米孔功能性控制方法及系统
CN113966403A (zh) * 2019-04-22 2022-01-21 奥特拉公司 样品中多核苷酸序列的分数丰度的多孔测定
CN110438203A (zh) * 2019-08-19 2019-11-12 上海纳米技术及应用国家工程研究中心有限公司 一种提高环介导等温扩增反应效率的方法及应用
CN110438203B (zh) * 2019-08-19 2023-11-28 上海纳米技术及应用国家工程研究中心有限公司 一种提高环介导等温扩增反应效率的方法及应用

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EP3253889A4 (en) 2018-09-26
AU2016215453A1 (en) 2017-08-10
JP2019092509A (ja) 2019-06-20
MX2017009766A (es) 2017-12-11
AU2020239812A1 (en) 2020-10-29
RU2017127990A3 (https=) 2019-03-04
US20180023115A1 (en) 2018-01-25
RU2017127990A (ru) 2019-03-04
KR20170106487A (ko) 2017-09-20
HK1245348A1 (zh) 2018-08-24
US11098348B2 (en) 2021-08-24
KR20190127983A (ko) 2019-11-13
CA2973753A1 (en) 2016-08-11
IL253272B (en) 2018-08-30
AU2018208656A1 (en) 2018-08-09
EP3253889A1 (en) 2017-12-13
IL253272A0 (en) 2017-09-03
JP2018505414A (ja) 2018-02-22

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