JP2017519519A5 - - Google Patents

Info

Publication number

JP2017519519A5

JP2017519519A5 JP2017510762A JP2017510762A JP2017519519A5 JP 2017519519 A5 JP2017519519 A5 JP 2017519519A5 JP 2017510762 A JP2017510762 A JP 2017510762A JP 2017510762 A JP2017510762 A JP 2017510762A JP 2017519519 A5 JP2017519519 A5 JP 2017519519A5

Authority

JP

Japan

Prior art keywords

seq

nicotine demethylase

mutations

tobacco plant

demethylase

Prior art date

2015-05-07

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

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• SNICXCGAKADSCV-JTQLQIEISA-N (-)-Nicotine Chemical compound CN1CCC[C@H]1C1=CC=CN=C1 SNICXCGAKADSCV-JTQLQIEISA-N 0.000 claims 55
• SNICXCGAKADSCV-UHFFFAOYSA-N nicotine Natural products CN1CCCC1C1=CC=CN=C1 SNICXCGAKADSCV-UHFFFAOYSA-N 0.000 claims 55
• 229960002715 nicotine Drugs 0.000 claims 55
• 230000035772 mutation Effects 0.000 claims 48
• 101000615488 Homo sapiens Methyl-CpG-binding domain protein 2 Proteins 0.000 claims 47
• 102100021299 Methyl-CpG-binding domain protein 2 Human genes 0.000 claims 47
• 244000061176 Nicotiana tabacum Species 0.000 claims 37
• 235000002637 Nicotiana tabacum Nutrition 0.000 claims 37
• 238000006467 substitution reaction Methods 0.000 claims 21
• 108090000623 proteins and genes Proteins 0.000 claims 19
• 241000196324 Embryophyta Species 0.000 claims 18
• MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 claims 12
• 239000000463 material Substances 0.000 claims 12
• 125000003607 serino group Chemical group [H]N([H])[C@]([H])(C(=O)[*])C(O[H])([H])[H] 0.000 claims 12
• MYKUKUCHPMASKF-VIFPVBQESA-N (S)-nornicotine Chemical compound C1CCN[C@@H]1C1=CC=CN=C1 MYKUKUCHPMASKF-VIFPVBQESA-N 0.000 claims 11
• MYKUKUCHPMASKF-UHFFFAOYSA-N Nornicotine Natural products C1CCNC1C1=CC=CN=C1 MYKUKUCHPMASKF-UHFFFAOYSA-N 0.000 claims 11
• 125000001500 prolyl group Chemical group [H]N1C([H])(C(=O)[*])C([H])([H])C([H])([H])C1([H])[H] 0.000 claims 11
• 125000000539 amino acid group Chemical group 0.000 claims 6
• 238000004925 denaturation Methods 0.000 claims 6
• 230000036425 denaturation Effects 0.000 claims 6
• 108010058669 N-Demethylating Oxidoreductases Proteins 0.000 claims 5
• 108091033319 polynucleotide Proteins 0.000 claims 5
• 102000040430 polynucleotide Human genes 0.000 claims 5
• 239000002157 polynucleotide Substances 0.000 claims 5
• 108020004705 Codon Proteins 0.000 claims 4
• 102000006374 N-Demethylating Oxidoreductases Human genes 0.000 claims 4
• 238000000034 method Methods 0.000 claims 4
• 125000000430 tryptophan group Chemical group [H]N([H])C(C(=O)O*)C([H])([H])C1=C([H])N([H])C2=C([H])C([H])=C([H])C([H])=C12 0.000 claims 4
• 230000000694 effects Effects 0.000 claims 3
• FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 2
• DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 claims 2
• ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 claims 2
• 229960001230 asparagine Drugs 0.000 claims 2
• 235000009582 asparagine Nutrition 0.000 claims 2
• 125000000613 asparagine group Chemical group N[C@@H](CC(N)=O)C(=O)* 0.000 claims 2
• 238000001035 drying Methods 0.000 claims 2
• 125000003630 glycyl group Chemical group [H]N([H])C([H])([H])C(*)=O 0.000 claims 2
• 125000001909 leucine group Chemical group [H]N(*)C(C(*)=O)C([H])([H])C(C([H])([H])[H])C([H])([H])[H] 0.000 claims 2
• 125000003588 lysine group Chemical group [H]N([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 claims 2
• 229930182817 methionine Natural products 0.000 claims 2
• 125000001360 methionine group Chemical group N[C@@H](CCSC)C(=O)* 0.000 claims 2
• 108020004707 nucleic acids Proteins 0.000 claims 2
• 102000039446 nucleic acids Human genes 0.000 claims 2
• 150000007523 nucleic acids Chemical class 0.000 claims 2
• 229920001184 polypeptide Polymers 0.000 claims 2
• 108090000765 processed proteins & peptides Proteins 0.000 claims 2
• 102000004196 processed proteins & peptides Human genes 0.000 claims 2
• 125000002987 valine group Chemical group [H]N([H])C([H])(C(*)=O)C([H])(C([H])([H])[H])C([H])([H])[H] 0.000 claims 2
• 239000002028 Biomass Substances 0.000 claims 1
• 241001044671 Piper tuberculatum Species 0.000 claims 1
• 102000055027 Protein Methyltransferases Human genes 0.000 claims 1
• 108700040121 Protein Methyltransferases Proteins 0.000 claims 1
• QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 claims 1
• 230000033228 biological regulation Effects 0.000 claims 1
• 238000006243 chemical reaction Methods 0.000 claims 1
• 235000019504 cigarettes Nutrition 0.000 claims 1
• 239000013604 expression vector Substances 0.000 claims 1
• 238000003306 harvesting Methods 0.000 claims 1
• 238000004519 manufacturing process Methods 0.000 claims 1
• 102220106441 rs149364972 Human genes 0.000 claims 1
• 238000012216 screening Methods 0.000 claims 1
• 239000000779 smoke Substances 0.000 claims 1
• 235000019505 tobacco product Nutrition 0.000 claims 1
• 239000013598 vector Substances 0.000 claims 1