MX337123B - Composiciones y metodos para minimizar la sintesis de nornicotina en el tabaco. - Google Patents
Composiciones y metodos para minimizar la sintesis de nornicotina en el tabaco.Info
- Publication number
- MX337123B MX337123B MX2012008060A MX2012008060A MX337123B MX 337123 B MX337123 B MX 337123B MX 2012008060 A MX2012008060 A MX 2012008060A MX 2012008060 A MX2012008060 A MX 2012008060A MX 337123 B MX337123 B MX 337123B
- Authority
- MX
- Mexico
- Prior art keywords
- tobacco
- nicotine
- nornicotine
- plant
- methods
- Prior art date
Links
- 235000002637 Nicotiana tabacum Nutrition 0.000 title abstract 8
- MYKUKUCHPMASKF-VIFPVBQESA-N (S)-nornicotine Chemical compound C1CCN[C@@H]1C1=CC=CN=C1 MYKUKUCHPMASKF-VIFPVBQESA-N 0.000 title abstract 7
- MYKUKUCHPMASKF-UHFFFAOYSA-N Nornicotine Natural products C1CCNC1C1=CC=CN=C1 MYKUKUCHPMASKF-UHFFFAOYSA-N 0.000 title abstract 7
- 238000000034 method Methods 0.000 title abstract 5
- 239000000203 mixture Substances 0.000 title abstract 4
- 241000208125 Nicotiana Species 0.000 title 1
- 230000015572 biosynthetic process Effects 0.000 title 1
- 238000003786 synthesis reaction Methods 0.000 title 1
- SNICXCGAKADSCV-JTQLQIEISA-N (-)-Nicotine Chemical compound CN1CCC[C@H]1C1=CC=CN=C1 SNICXCGAKADSCV-JTQLQIEISA-N 0.000 abstract 10
- SNICXCGAKADSCV-UHFFFAOYSA-N nicotine Natural products CN1CCCC1C1=CC=CN=C1 SNICXCGAKADSCV-UHFFFAOYSA-N 0.000 abstract 10
- 229960002715 nicotine Drugs 0.000 abstract 10
- 101000615488 Homo sapiens Methyl-CpG-binding domain protein 2 Proteins 0.000 abstract 7
- 244000061176 Nicotiana tabacum Species 0.000 abstract 7
- 241000196324 Embryophyta Species 0.000 abstract 6
- 102100021299 Methyl-CpG-binding domain protein 2 Human genes 0.000 abstract 4
- 230000035772 mutation Effects 0.000 abstract 4
- 238000006243 chemical reaction Methods 0.000 abstract 3
- 235000019505 tobacco product Nutrition 0.000 abstract 3
- 230000000711 cancerogenic effect Effects 0.000 abstract 2
- 231100000315 carcinogenic Toxicity 0.000 abstract 2
- 230000002503 metabolic effect Effects 0.000 abstract 2
- 108700028369 Alleles Proteins 0.000 abstract 1
- 239000002207 metabolite Substances 0.000 abstract 1
- XKABJYQDMJTNGQ-VIFPVBQESA-N n-nitrosonornicotine Chemical compound O=NN1CCC[C@H]1C1=CC=CN=C1 XKABJYQDMJTNGQ-VIFPVBQESA-N 0.000 abstract 1
- 102000040430 polynucleotide Human genes 0.000 abstract 1
- 108091033319 polynucleotide Proteins 0.000 abstract 1
- 239000002157 polynucleotide Substances 0.000 abstract 1
- 229920001184 polypeptide Polymers 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 1
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 239000000779 smoke Substances 0.000 abstract 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
- A01H5/12—Leaves
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
- A01H6/82—Solanaceae, e.g. pepper, tobacco, potato, tomato or eggplant
- A01H6/823—Nicotiana, e.g. tobacco
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B13/00—Tobacco for pipes, for cigars, e.g. cigar inserts, or for cigarettes; Chewing tobacco; Snuff
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
- C12N15/8223—Vegetative tissue-specific promoters
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
- C12N9/0073—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen 1.14.13
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/13—Plant traits
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Abstract
La presente invención se refiere a composiciones y métodos para reducir el nivel de nornicotina y N´-nitrosonornicotina (NNN) en plantas de tabaco y partes de planta de las mismas. Las composiciones comprenden polinucleótidos y polipéptidos aislados para nicotina demetilasas, CYP82E10, específicas de raíz y variantes de las mismas, que están involucradas en la conversión metabólica de nicotina a nornicotina en estas plantas. Las composiciones de la invención también incluyen planta de tabaco, o partes de planta de las mismas, que comprenden una mutación en un gen que codifica una nicotina demetilasa CYPS2E10, en donde la mutación da por resultado la expresión o función reducida de la nicotina demetilasa CYP82E10. También se proporcionan semilla de estas plantas de tabaco, o progenie de las mismas, y productos de tabaco preparados a partir de las plantas de tabaco de la invención, o a partir de partes de planta o progenie de las mismas. También se proporcionan métodos para reducir el nivel de nornicotina, o reducir la tasa de conversión de nicotina a nornicotina, en una planta de tabaco, o parte de planta de la misma. Los métodos comprenden introducir en el genoma de una planta de tabaco una mutación dentro de por lo menos un alelo de cada uno de los tres genes de nicotina demetilasa, en donde la mutación reduce la expresión del gen de nicotina demetilasa y en donde un primero de estos genes de nicotina demetilasa codificada una nicotina demetilasa específica de raíz involucrada en la conversión metabólica de nicotina a nornicotina en una planta de tabaco una parte de planta de la misma. Los métodos encuentran uso en la producción de productos de tabaco que tienen niveles reducidos de nornicotina y su metabolito carcinogénico, NNN, y de esta manera potencial carcinogénico reducido para individuos que consumen estos productos de tabaco o expuestos al humo secundario derivado de estos productos.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US29567110P | 2010-01-15 | 2010-01-15 | |
PCT/US2011/021088 WO2011088180A1 (en) | 2010-01-15 | 2011-01-13 | Compositions and methods for minimizing nornicotine synthesis in tobacco |
Publications (2)
Publication Number | Publication Date |
---|---|
MX2012008060A MX2012008060A (es) | 2013-01-14 |
MX337123B true MX337123B (es) | 2016-02-12 |
Family
ID=43722405
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2012008060A MX337123B (es) | 2010-01-15 | 2011-01-13 | Composiciones y metodos para minimizar la sintesis de nornicotina en el tabaco. |
Country Status (14)
Country | Link |
---|---|
US (6) | US9247706B2 (es) |
EP (1) | EP2524044A1 (es) |
JP (1) | JP6055311B2 (es) |
KR (1) | KR101611847B1 (es) |
CN (1) | CN102858983B (es) |
AR (2) | AR080636A1 (es) |
AU (1) | AU2011205282B2 (es) |
BR (1) | BR112012017565A2 (es) |
CA (1) | CA2786813C (es) |
EA (1) | EA033565B1 (es) |
MX (1) | MX337123B (es) |
PH (1) | PH12017500180B1 (es) |
WO (1) | WO2011088180A1 (es) |
ZA (1) | ZA201205426B (es) |
Families Citing this family (59)
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US10266836B2 (en) | 2001-11-13 | 2019-04-23 | U.S. Smokeless Tobacco Company Llc | Tobacco nicotine demethylase genomic clone and uses thereof |
ATE530656T1 (de) | 2005-02-23 | 2011-11-15 | Univ North Carolina State | Veränderung des alkaloidgehaltes in tabak durch modifikation spezifischer cytochrome p450 gene. |
CN101939431B (zh) | 2007-11-12 | 2015-01-28 | 北卡罗来纳州立大学 | 通过修饰特定细胞色素p450基因改变烟草生物碱含量 |
EA033565B1 (ru) | 2010-01-15 | 2019-11-05 | Univ North Carolina State | Композиции и способы, предназначенные для минимизации синтеза норникотина в растениях табака |
KR20140004760A (ko) | 2011-02-28 | 2014-01-13 | 노쓰 캐롤라이나 스테이트 유니버시티 | 담배 근교배 식물 ncbex1f, ncbex1ms 및 nc ex90 |
KR20140034160A (ko) | 2011-02-28 | 2014-03-19 | 알트리아 클라이언트 서비시스 인코포레이티드 | 담배 근친교배 식물체 albex1f 및 albex1ms |
CA2894955C (en) | 2012-12-21 | 2023-10-31 | Philip Morris Products S.A. | Tobacco specific nitrosamine reduction in plants |
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US10375910B2 (en) | 2013-03-15 | 2019-08-13 | Altria Client Services Llc | Development of tobacco varieties with no or significantly reduced anatabine content |
US9710787B2 (en) * | 2013-07-31 | 2017-07-18 | The Board Of Trustees Of The Leland Stanford Junior University | Systems and methods for representing, diagnosing, and recommending interaction sequences |
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CN106659232A (zh) * | 2014-03-03 | 2017-05-10 | 北卡罗莱纳州立大学 | 烟草近交和杂种植物以及由其制得的烟草产品 |
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US10415050B2 (en) | 2014-05-08 | 2019-09-17 | Philip Morris Products S.A. | Reduction of nicotine to nornicotine conversion in plants |
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BR112017023719A2 (pt) * | 2015-05-05 | 2018-07-17 | Univ North Carolina State | planta de tabaco, parte de planta e/ou célula de planta, semente, cultura de planta, produto de tabaco, método para reduzir pon e/ou nnk, método de produção de uma planta e método para produzir um produto de tabaco |
CN116250483B (zh) * | 2015-06-26 | 2024-01-30 | 奥驰亚客户服务公司 | 用于生产烟草植物的组合物和方法以及具有改变的生物碱含量的制品 |
CN105602961B (zh) * | 2015-09-07 | 2018-10-23 | 中国农业科学院烟草研究所 | 突变的烤烟cyp82e4基因及其应用 |
US11213004B2 (en) | 2016-01-29 | 2022-01-04 | Philip Morris Products S.A. | Reducing cadmium accumulation in field grown tobacco plants |
CN117089526A (zh) | 2016-03-11 | 2023-11-21 | 奥驰亚客户服务有限公司 | 用于生产烟杈减少或消除的烟草植物和制品的组合物和方法 |
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CN108424922B (zh) * | 2018-04-20 | 2021-04-16 | 云南省烟草农业科学研究院 | 一种降低尼古丁转化率的cyp82e10基因错义突变体m271及其应用 |
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JP2022515435A (ja) | 2018-12-30 | 2022-02-18 | フィリップ・モーリス・プロダクツ・ソシエテ・アノニム | 硝酸レダクターゼの突然変異による植物における硝酸塩レベルの調節 |
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