CA2944965A1 - Reduction of nicotine to nornicotine conversion in plants - Google Patents
Reduction of nicotine to nornicotine conversion in plants Download PDFInfo
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- CA2944965A1 CA2944965A1 CA2944965A CA2944965A CA2944965A1 CA 2944965 A1 CA2944965 A1 CA 2944965A1 CA 2944965 A CA2944965 A CA 2944965A CA 2944965 A CA2944965 A CA 2944965A CA 2944965 A1 CA2944965 A1 CA 2944965A1
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- seq
- plant
- nicotine demethylase
- mutations
- sequence
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
- C12N9/0073—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen 1.14.13
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
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- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/13—Plant traits
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- C12Q2600/156—Polymorphic or mutational markers
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- C12Y—ENZYMES
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- C12Y114/13—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen (1.14.13)
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- Nutrition Science (AREA)
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- Virology (AREA)
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- Botany (AREA)
- Immunology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
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Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP14167598 | 2014-05-08 | ||
| EP14167598.3 | 2014-05-08 | ||
| EP14001645.2 | 2014-05-09 | ||
| EP14001645 | 2014-05-09 | ||
| PCT/EP2015/060124 WO2015169927A1 (en) | 2014-05-08 | 2015-05-07 | Reduction of nicotine to nornicotine conversion in plants |
Publications (1)
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| CA2944965A1 true CA2944965A1 (en) | 2015-11-12 |
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| Application Number | Title | Priority Date | Filing Date |
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| CA2944965A Abandoned CA2944965A1 (en) | 2014-05-08 | 2015-05-07 | Reduction of nicotine to nornicotine conversion in plants |
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| Country | Link |
|---|---|
| US (1) | US10415050B2 (enExample) |
| EP (1) | EP3140404A1 (enExample) |
| JP (1) | JP6871158B2 (enExample) |
| KR (1) | KR102442802B1 (enExample) |
| CN (1) | CN106459923B (enExample) |
| BR (1) | BR112016025234A2 (enExample) |
| CA (1) | CA2944965A1 (enExample) |
| MX (1) | MX2016014635A (enExample) |
| PH (1) | PH12016501718A1 (enExample) |
| RU (1) | RU2733837C2 (enExample) |
| WO (1) | WO2015169927A1 (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112639087A (zh) * | 2018-08-02 | 2021-04-09 | 安蒂多特疗法公司 | 新型尼古丁降解酶变体 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106636146B (zh) * | 2017-02-16 | 2019-09-13 | 云南省烟草农业科学研究院 | 一种降低尼古丁转化率的cyp82e5基因突变体及其应用 |
| US11046969B2 (en) * | 2017-05-24 | 2021-06-29 | Epiplanta Biotech Ltd. | Transgenic plant and the method for producing the same |
| WO2018222667A1 (en) * | 2017-05-31 | 2018-12-06 | 22Nd Century Limited, Llc | Genome editing methods for producing low-nicotine tobacco products |
| US11773401B2 (en) | 2017-12-07 | 2023-10-03 | University Of Kentucky Research Foundation | bZIP transcription factors regulate conversion of nicotine to nornicotine |
| CN108192900B (zh) * | 2018-01-16 | 2021-11-30 | 西南大学 | 烟草烟碱去甲基化酶基因cyp82e4的编辑失活载体及其应用 |
| CN111801423A (zh) | 2018-03-28 | 2020-10-20 | 菲利普莫里斯生产公司 | 调节植物中的氨基酸含量 |
| BR112020017430A2 (pt) | 2018-03-28 | 2021-01-19 | Philip Morris Products S.A. | Modulação do teor de açúcar redutor em uma planta |
| CN108424921B (zh) * | 2018-04-20 | 2021-04-20 | 云南省烟草农业科学研究院 | 一种降低尼古丁转化率的cyp82e10基因错义突变体m594及其应用 |
| KR102851176B1 (ko) * | 2018-07-26 | 2025-08-28 | 알트리아 클라이언트 서비시즈 엘엘씨 | 알칼로이드 수준이 변경된 담배 식물 및 제품을 생산하기 위한 pmt 공학에 기초한 조성물 및 방법 |
| CN113271768B (zh) * | 2018-12-30 | 2024-02-06 | 菲利普莫里斯生产公司 | 经由硝酸盐还原酶的突变来调节植物中的硝酸盐水平 |
| BR112022005552A2 (pt) | 2019-10-01 | 2022-06-28 | Philip Morris Products Sa | Modulação da redução do teor de açúcar em uma planta (inv) |
| US12419264B2 (en) | 2019-10-01 | 2025-09-23 | Philip Morris Products S.A. | Modulating sugar and amino acid content in a plant (SULTR3) |
| US20240218386A1 (en) * | 2019-12-03 | 2024-07-04 | Altria Client Services Llc | Compositions and Methods for Producing Tobacco Plants and Products Having Altered Alkaloid Levels |
| US11536685B2 (en) | 2020-02-06 | 2022-12-27 | Trustees Of Boston University | High throughput assay for identifying microbial redox enzymes |
| WO2021158973A1 (en) | 2020-02-06 | 2021-08-12 | Trustees Of Boston University | Enzyme-based electrochemical nicotine biosensor |
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| CN111505158B (zh) * | 2020-05-09 | 2022-09-23 | 云南省烟草农业科学研究院 | 一种基于孵化处理的尼古丁转化烟株色质快速筛查方法 |
| WO2023036691A1 (en) | 2021-09-10 | 2023-03-16 | Philip Morris Products S.A. | Modulating alkaloid profiles in nicotiana tabacum |
| CN113980978B (zh) * | 2021-11-22 | 2023-12-26 | 云南中烟工业有限责任公司 | 一种烟草烟碱转运蛋白相关的基因及其应用 |
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| WO2023117701A1 (en) | 2021-12-21 | 2023-06-29 | Philip Morris Products S.A. | Modulation of nicotine production by alteration of nicotinamidase expression or function in plants |
| CN114540382B (zh) * | 2022-02-18 | 2023-07-18 | 贵州省烟草科学研究院 | 烟草镉转运基因NtPLA1及应用 |
| JP2025533986A (ja) | 2022-10-13 | 2025-10-09 | フィリップ・モーリス・プロダクツ・ソシエテ・アノニム | Ntp2を調節することによって葉バイオマスおよび窒素利用効率を増加させる |
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| KR20250139371A (ko) | 2023-02-02 | 2025-09-23 | 필립모리스 프로덕츠 에스.에이. | 당 수송체의 조절 |
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| EP2087123B1 (en) | 2006-10-13 | 2011-08-10 | North Carolina State University | Alteration of tobacco alkaloid content through modification of specific cytochrome p450 genes |
| RU2324737C1 (ru) * | 2006-10-18 | 2008-05-20 | Институт цитологии и генетики Сибирского отделения Российской академии наук (СО РАН) | Способ получения трансгенных растений табака с повышенным содержанием пролина |
| US8319011B2 (en) | 2006-12-15 | 2012-11-27 | U.S. Smokeless Tobacco Company Llc | Tobacco plants having reduced nicotine demethylase activity |
| BRPI0820042B1 (pt) * | 2007-11-12 | 2020-05-19 | North Carolina State University | método de obtenção de uma planta de tabaco, ou célula ou parte desta, tendo níveis reduzidos de nornicotina, produto de tabaco, método para fabricar um produto de tabaco, polinucleotídeo isolado, cassete de expressão, polipetpídeo isolado e método de obtenção de uma planta, ou parte de planta desta, do gênero nicotiana |
| EP2231861B1 (en) | 2007-12-13 | 2014-10-29 | Philip Morris Products S.A. | Transgenic plants modified for reduced cadmium transport, derivative products, and related methods |
| US7883371B1 (en) | 2009-07-22 | 2011-02-08 | Hon Hai Precision Ind. Co., Ltd. | Electrical connector with improved contact footprints |
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| EP3447065A1 (en) | 2010-09-03 | 2019-02-27 | Philip Morris Products S.A. | Heavy metal reduction in planta |
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- 2015-05-07 MX MX2016014635A patent/MX2016014635A/es unknown
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112639087A (zh) * | 2018-08-02 | 2021-04-09 | 安蒂多特疗法公司 | 新型尼古丁降解酶变体 |
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|---|---|
| RU2733837C2 (ru) | 2020-10-07 |
| CN106459923B (zh) | 2020-08-11 |
| MX2016014635A (es) | 2017-02-23 |
| US20170130239A1 (en) | 2017-05-11 |
| PH12016501718A1 (en) | 2016-11-07 |
| JP2017519519A (ja) | 2017-07-20 |
| RU2016144409A3 (enExample) | 2018-11-29 |
| RU2016144409A (ru) | 2018-06-13 |
| BR112016025234A2 (pt) | 2017-12-12 |
| KR102442802B1 (ko) | 2022-09-14 |
| KR20170002444A (ko) | 2017-01-06 |
| WO2015169927A1 (en) | 2015-11-12 |
| CN106459923A (zh) | 2017-02-22 |
| US10415050B2 (en) | 2019-09-17 |
| JP6871158B2 (ja) | 2021-05-12 |
| EP3140404A1 (en) | 2017-03-15 |
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