JP2017517266A5 - - Google Patents

Info

Publication number

JP2017517266A5

JP2017517266A5 JP2016572447A JP2016572447A JP2017517266A5 JP 2017517266 A5 JP2017517266 A5 JP 2017517266A5 JP 2016572447 A JP2016572447 A JP 2016572447A JP 2016572447 A JP2016572447 A JP 2016572447A JP 2017517266 A5 JP2017517266 A5 JP 2017517266A5

Authority

JP

Japan

Prior art keywords

triphosphate

rna molecule

sequence

bioreactor

ntp

Prior art date

2015-06-10

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
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• 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 18
• 238000000034 method Methods 0.000 claims 16
• 239000002773 nucleotide Substances 0.000 claims 14
• 125000003729 nucleotide group Chemical group 0.000 claims 14
• 239000001226 triphosphate Substances 0.000 claims 14
• 235000011178 triphosphate Nutrition 0.000 claims 14
• 239000002342 ribonucleoside Substances 0.000 claims 10
• -1 ribonucleoside triphosphates Chemical class 0.000 claims 9
• 239000011541 reaction mixture Substances 0.000 claims 8
• UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 claims 8
• NYHBQMYGNKIUIF-UUOKFMHZSA-N Guanosine Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O NYHBQMYGNKIUIF-UUOKFMHZSA-N 0.000 claims 7
• 238000013518 transcription Methods 0.000 claims 7
• 230000035897 transcription Effects 0.000 claims 7
• 238000000338 in vitro Methods 0.000 claims 6
• 239000000203 mixture Substances 0.000 claims 6
• 238000005457 optimization Methods 0.000 claims 6
• 239000002777 nucleoside Substances 0.000 claims 5
• 230000015572 biosynthetic process Effects 0.000 claims 4
• 150000002500 ions Chemical class 0.000 claims 4
• 238000003786 synthesis reaction Methods 0.000 claims 4
• PGAVKCOVUIYSFO-XVFCMESISA-N UTP Chemical compound O[C@@H]1[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O[C@H]1N1C(=O)NC(=O)C=C1 PGAVKCOVUIYSFO-XVFCMESISA-N 0.000 claims 3
• 238000001914 filtration Methods 0.000 claims 3
• 239000012528 membrane Substances 0.000 claims 3
• 230000002194 synthesizing effect Effects 0.000 claims 3
• PGAVKCOVUIYSFO-UHFFFAOYSA-N uridine-triphosphate Natural products OC1C(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)OC1N1C(=O)NC(=O)C=C1 PGAVKCOVUIYSFO-UHFFFAOYSA-N 0.000 claims 3
• 102000004163 DNA-directed RNA polymerases Human genes 0.000 claims 2
• 108090000626 DNA-directed RNA polymerases Proteins 0.000 claims 2
• 239000004372 Polyvinyl alcohol Substances 0.000 claims 2
• 239000007983 Tris buffer Substances 0.000 claims 2
• 239000000872 buffer Substances 0.000 claims 2
• VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 claims 2
• 229920000110 poly(aryl ether sulfone) Polymers 0.000 claims 2
• 229920003229 poly(methyl methacrylate) Polymers 0.000 claims 2
• 229920002492 poly(sulfone) Polymers 0.000 claims 2
• 229920002239 polyacrylonitrile Polymers 0.000 claims 2
• 239000004926 polymethyl methacrylate Substances 0.000 claims 2
• 229920002451 polyvinyl alcohol Polymers 0.000 claims 2
• GKVHYBAWZAYQDO-XVFCMESISA-N [[(2r,3s,4r,5r)-3,4-dihydroxy-5-(2-oxo-4-sulfanylidenepyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate Chemical compound O1[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@@H](O)[C@@H]1N1C(=O)NC(=S)C=C1 GKVHYBAWZAYQDO-XVFCMESISA-N 0.000 claims 1
• KHYOUGAATNYCAZ-XVFCMESISA-N [[(2r,3s,4r,5r)-3,4-dihydroxy-5-(4-oxo-2-sulfanylidenepyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate Chemical compound O1[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@@H](O)[C@@H]1N1C(=S)NC(=O)C=C1 KHYOUGAATNYCAZ-XVFCMESISA-N 0.000 claims 1
• YIJVOACVHQZMKI-JXOAFFINSA-N [[(2r,3s,4r,5r)-5-(4-amino-5-methyl-2-oxopyrimidin-1-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate Chemical compound O=C1N=C(N)C(C)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 YIJVOACVHQZMKI-JXOAFFINSA-N 0.000 claims 1
• VEWJOCYCKIZKKV-GBNDHIKLSA-N [[(2r,3s,4r,5s)-5-(2,4-dioxo-1h-pyrimidin-5-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate Chemical compound O[C@@H]1[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O[C@H]1C1=CNC(=O)NC1=O VEWJOCYCKIZKKV-GBNDHIKLSA-N 0.000 claims 1
• 239000001913 cellulose Substances 0.000 claims 1
• 229920002678 cellulose Polymers 0.000 claims 1
• 238000006243 chemical reaction Methods 0.000 claims 1
• 239000001177 diphosphate Substances 0.000 claims 1
• 235000011180 diphosphates Nutrition 0.000 claims 1
• 230000000977 initiatory effect Effects 0.000 claims 1
• 239000007788 liquid Substances 0.000 claims 1
• 108020004999 messenger RNA Proteins 0.000 claims 1
• 108091027963 non-coding RNA Proteins 0.000 claims 1
• 102000042567 non-coding RNA Human genes 0.000 claims 1
• 230000003287 optical effect Effects 0.000 claims 1
• 239000004627 regenerated cellulose Substances 0.000 claims 1
• 239000011342 resin composition Substances 0.000 claims 1
• 239000007787 solid Substances 0.000 claims 1
• LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims 1