JP2016535983A5 - - Google Patents
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- Publication number
- JP2016535983A5 JP2016535983A5 JP2016521773A JP2016521773A JP2016535983A5 JP 2016535983 A5 JP2016535983 A5 JP 2016535983A5 JP 2016521773 A JP2016521773 A JP 2016521773A JP 2016521773 A JP2016521773 A JP 2016521773A JP 2016535983 A5 JP2016535983 A5 JP 2016535983A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- polymerase
- nucleotide
- binding site
- chemical modification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 22
- 150000007523 nucleic acids Chemical class 0.000 claims 19
- 108020004707 nucleic acids Proteins 0.000 claims 16
- 102000039446 nucleic acids Human genes 0.000 claims 16
- 239000002773 nucleotide Substances 0.000 claims 8
- 125000003729 nucleotide group Chemical group 0.000 claims 8
- 238000007385 chemical modification Methods 0.000 claims 7
- 239000003153 chemical reaction reagent Substances 0.000 claims 6
- 238000012217 deletion Methods 0.000 claims 5
- 230000037430 deletion Effects 0.000 claims 5
- 230000027455 binding Effects 0.000 claims 4
- 102000004169 proteins and genes Human genes 0.000 claims 3
- 108090000623 proteins and genes Proteins 0.000 claims 3
- 150000003384 small molecules Chemical class 0.000 claims 3
- KJMRWDHBVCNLTQ-UHFFFAOYSA-N N-methylisatoic anhydride Chemical compound C1=CC=C2C(=O)OC(=O)N(C)C2=C1 KJMRWDHBVCNLTQ-UHFFFAOYSA-N 0.000 claims 2
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 2
- 230000000295 complement effect Effects 0.000 claims 2
- 239000012039 electrophile Substances 0.000 claims 2
- 230000009149 molecular binding Effects 0.000 claims 2
- 108020004635 Complementary DNA Proteins 0.000 claims 1
- HMFHBZSHGGEWLO-SOOFDHNKSA-N D-ribofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-SOOFDHNKSA-N 0.000 claims 1
- 102100034343 Integrase Human genes 0.000 claims 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims 1
- PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 claims 1
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 claims 1
- 239000012472 biological sample Substances 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 claims 1
- 238000004590 computer program Methods 0.000 claims 1
- 238000001514 detection method Methods 0.000 claims 1
- 230000003993 interaction Effects 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 238000012163 sequencing technique Methods 0.000 claims 1
- 238000000547 structure data Methods 0.000 claims 1
- 238000003786 synthesis reaction Methods 0.000 claims 1
- 230000002194 synthesizing effect Effects 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361887614P | 2013-10-07 | 2013-10-07 | |
| US61/887,614 | 2013-10-07 | ||
| PCT/US2014/059489 WO2015054247A1 (en) | 2013-10-07 | 2014-10-07 | Detection of chemical modifications in nucleic acids |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016535983A JP2016535983A (ja) | 2016-11-24 |
| JP2016535983A5 true JP2016535983A5 (enExample) | 2017-11-02 |
| JP6612220B2 JP6612220B2 (ja) | 2019-11-27 |
Family
ID=52813579
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016521773A Active JP6612220B2 (ja) | 2013-10-07 | 2014-10-07 | 核酸における化学修飾の検出 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US10240188B2 (enExample) |
| EP (1) | EP3055413B1 (enExample) |
| JP (1) | JP6612220B2 (enExample) |
| ES (1) | ES2791873T3 (enExample) |
| WO (1) | WO2015054247A1 (enExample) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MX2019005149A (es) * | 2016-11-07 | 2019-06-24 | nanoSUR LLC | Arn bicatenario con modificaciones quimicas postranscripcionales. |
| US12195788B2 (en) * | 2017-01-12 | 2025-01-14 | University Of Central Florida Research Foundation, Inc. | High-throughput and single nucleotide resolution techniques for the determination of RNA post-transcriptional modifications |
| RU2659025C1 (ru) * | 2017-06-14 | 2018-06-26 | Общество с ограниченной ответственностью "ЛЭНДИГРАД" | Способы кодирования и декодирования информации |
| CN109517889B (zh) | 2017-09-18 | 2022-04-05 | 苏州吉赛基因测序科技有限公司 | 一种基于高通量测序分析寡核苷酸序列杂质的方法及应用 |
| RU2020112854A (ru) | 2017-11-30 | 2021-12-30 | Арракис Терапьютикс, Инк. | Фотозонды, связывающие нуклеиновые кислоты, и способы их применения |
| CN114438168B (zh) * | 2020-11-05 | 2024-10-22 | 寻鲸生科(北京)智能技术有限公司 | 一种全转录组水平rna结构检测方法及其应用 |
| US20240052412A1 (en) * | 2020-11-05 | 2024-02-15 | Tsinghua University | Method for detecting rna structure at whole transcriptome level and use thereof |
| CN114507721B (zh) * | 2020-11-16 | 2024-04-09 | 寻鲸生科(北京)智能技术有限公司 | 一种全转录组rna结构探测的方法及其应用 |
| JP7141165B1 (ja) * | 2020-11-18 | 2022-09-22 | 株式会社イクスフォレストセラピューティクス | 変異プロファイリングのためのｒｎａプローブ及びその使用 |
| WO2023023584A2 (en) | 2021-08-19 | 2023-02-23 | Eclipse Bioinnovations, Inc. | Methods for detecting rna binding protein complexes |
| WO2023235820A1 (en) * | 2022-06-03 | 2023-12-07 | Eclipse Bioinnovations, Inc. | Methods for obtaining rna secondary structure of lnp-encapsulated rna |
| WO2025137453A1 (en) * | 2023-12-20 | 2025-06-26 | New England Biolabs, Inc. | Compositions, methods, kits, and instruments for analyzing rna structure |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001094626A1 (en) | 1999-04-30 | 2001-12-13 | Cyclops Genome Sciences Limited | Polynucleotides |
| US20070196832A1 (en) * | 2006-02-22 | 2007-08-23 | Efcavitch J William | Methods for mutation detection |
| US8318424B2 (en) | 2006-06-05 | 2012-11-27 | The University Of North Carolina At Chapel Hill | High-throughput RNA structure analysis |
| EP2053131A1 (en) | 2007-10-19 | 2009-04-29 | Ludwig-Maximilians-Universität München | Method for determining methylation at deoxycytosine residues |
| US20130288917A1 (en) | 2010-10-21 | 2013-10-31 | The Board Of Trustees Of The Leland Stanford Junior University | Rapid High Resolution, High Throughput RNA Structure, RNA-Macromolecular Interaction, and RNA-Small Molecule Interaction Mapping |
-
2014
- 2014-10-07 JP JP2016521773A patent/JP6612220B2/ja active Active
- 2014-10-07 US US15/027,813 patent/US10240188B2/en active Active
- 2014-10-07 EP EP14851691.7A patent/EP3055413B1/en active Active
- 2014-10-07 ES ES14851691T patent/ES2791873T3/es active Active
- 2014-10-07 WO PCT/US2014/059489 patent/WO2015054247A1/en not_active Ceased
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