JP6612220B2 - 核酸における化学修飾の検出 - Google Patents

核酸における化学修飾の検出 Download PDF

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JP6612220B2
JP6612220B2 JP2016521773A JP2016521773A JP6612220B2 JP 6612220 B2 JP6612220 B2 JP 6612220B2 JP 2016521773 A JP2016521773 A JP 2016521773A JP 2016521773 A JP2016521773 A JP 2016521773A JP 6612220 B2 JP6612220 B2 JP 6612220B2
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rna
shape
group
nucleotides
reactivity
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JP2016535983A5 (enExample
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エム ウイークス ケビン
ジークフリード ナタン
ホマン フィリップ
ブサン スティーブン
ブイ ファボロフ オレグ
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University of North Carolina at Chapel Hill
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2016521773A 2013-10-07 2014-10-07 核酸における化学修飾の検出 Active JP6612220B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361887614P 2013-10-07 2013-10-07
US61/887,614 2013-10-07
PCT/US2014/059489 WO2015054247A1 (en) 2013-10-07 2014-10-07 Detection of chemical modifications in nucleic acids

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JP2016535983A JP2016535983A (ja) 2016-11-24
JP2016535983A5 JP2016535983A5 (enExample) 2017-11-02
JP6612220B2 true JP6612220B2 (ja) 2019-11-27

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JP2016521773A Active JP6612220B2 (ja) 2013-10-07 2014-10-07 核酸における化学修飾の検出

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US (1) US10240188B2 (enExample)
EP (1) EP3055413B1 (enExample)
JP (1) JP6612220B2 (enExample)
ES (1) ES2791873T3 (enExample)
WO (1) WO2015054247A1 (enExample)

Cited By (1)

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Publication number Priority date Publication date Assignee Title
WO2022107814A1 (ja) * 2020-11-18 2022-05-27 株式会社イクスフォレストセラピューティクス 変異プロファイリングのためのｒｎａプローブ及びその使用

Families Citing this family (11)

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MX2019005149A (es) * 2016-11-07 2019-06-24 nanoSUR LLC Arn bicatenario con modificaciones quimicas postranscripcionales.
US12195788B2 (en) * 2017-01-12 2025-01-14 University Of Central Florida Research Foundation, Inc. High-throughput and single nucleotide resolution techniques for the determination of RNA post-transcriptional modifications
RU2659025C1 (ru) * 2017-06-14 2018-06-26 Общество с ограниченной ответственностью "ЛЭНДИГРАД" Способы кодирования и декодирования информации
CN109517889B (zh) 2017-09-18 2022-04-05 苏州吉赛基因测序科技有限公司 一种基于高通量测序分析寡核苷酸序列杂质的方法及应用
RU2020112854A (ru) 2017-11-30 2021-12-30 Арракис Терапьютикс, Инк. Фотозонды, связывающие нуклеиновые кислоты, и способы их применения
CN114438168B (zh) * 2020-11-05 2024-10-22 寻鲸生科(北京)智能技术有限公司 一种全转录组水平rna结构检测方法及其应用
US20240052412A1 (en) * 2020-11-05 2024-02-15 Tsinghua University Method for detecting rna structure at whole transcriptome level and use thereof
CN114507721B (zh) * 2020-11-16 2024-04-09 寻鲸生科(北京)智能技术有限公司 一种全转录组rna结构探测的方法及其应用
WO2023023584A2 (en) 2021-08-19 2023-02-23 Eclipse Bioinnovations, Inc. Methods for detecting rna binding protein complexes
WO2023235820A1 (en) * 2022-06-03 2023-12-07 Eclipse Bioinnovations, Inc. Methods for obtaining rna secondary structure of lnp-encapsulated rna
WO2025137453A1 (en) * 2023-12-20 2025-06-26 New England Biolabs, Inc. Compositions, methods, kits, and instruments for analyzing rna structure

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Publication number Priority date Publication date Assignee Title
WO2001094626A1 (en) 1999-04-30 2001-12-13 Cyclops Genome Sciences Limited Polynucleotides
US20070196832A1 (en) * 2006-02-22 2007-08-23 Efcavitch J William Methods for mutation detection
US8318424B2 (en) 2006-06-05 2012-11-27 The University Of North Carolina At Chapel Hill High-throughput RNA structure analysis
EP2053131A1 (en) 2007-10-19 2009-04-29 Ludwig-Maximilians-Universität München Method for determining methylation at deoxycytosine residues
US20130288917A1 (en) 2010-10-21 2013-10-31 The Board Of Trustees Of The Leland Stanford Junior University Rapid High Resolution, High Throughput RNA Structure, RNA-Macromolecular Interaction, and RNA-Small Molecule Interaction Mapping

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022107814A1 (ja) * 2020-11-18 2022-05-27 株式会社イクスフォレストセラピューティクス 変異プロファイリングのためのｒｎａプローブ及びその使用
JP7141165B1 (ja) * 2020-11-18 2022-09-22 株式会社イクスフォレストセラピューティクス 変異プロファイリングのためのｒｎａプローブ及びその使用
CN116234903A (zh) * 2020-11-18 2023-06-06 xFOREST制药株式会社 用于突变谱分析的rna探针及其用途
CN116234903B (zh) * 2020-11-18 2024-06-11 xFOREST制药株式会社 用于突变谱分析的rna探针及其用途

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US10240188B2 (en) 2019-03-26
ES2791873T3 (es) 2020-11-06
EP3055413A4 (en) 2017-04-05
EP3055413A1 (en) 2016-08-17
WO2015054247A1 (en) 2015-04-16
US20160244818A1 (en) 2016-08-25
EP3055413B1 (en) 2020-01-08
JP2016535983A (ja) 2016-11-24

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