JP2016187340A - Klk3、psca、またはfolh1抗原を含む組成物および方法 - Google Patents
Klk3、psca、またはfolh1抗原を含む組成物および方法 Download PDFInfo
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Abstract
Description
MWVPVVFLTLSVTWIGAAPLILSRIVGGWECEKHSQPWQVLVASRGRAVCGGVLVHPQWVLTAAHCIRNKSVILLGRHSLFHPEDTGQVFQVSHSFPHPLYDMSLLKNRFLRPGDDSSHDLMLLRLSEPAELTDAVKVMDLPTQEPALGTTCYASGWGSIEPEEFLTPKKLQCVDLHVISNDVCAQVHPQKVTKFMLCAGRWTGGKSTCSGDSGGPLVCNGVLQGITSWGSEPCALPERPSLYTKVVHYRKWIKDTIVANP(配列番号:25;GenBankアクセス番号X14810)。もう1つの実施態様において、KLK3蛋白質は配列番号:25のホモログである。もう1つの実施態様において、KLK3蛋白質は配列番号:25の変種である。もう1つの実施態様において、KLK3蛋白質は配列番号:25のイソ形態である。もう1つの実施態様において、KLK3蛋白質は配列番号:25の断片である。各可能性は本発明の別の実施態様を表わす。
ggtgtcttaggcacactggtcttggagtgcaaaggatctaggcacgtgaggctttgtatgaagaatcggggatcgtacccaccccctgtttctgtttcatcctgggcatgtctcctctgcctttgtcccctagatgaagtctccatgagctacaagggcctggtgcatccagggtgatctagtaattgcagaacagcaagtgctagctctccctccccttccacagctctgggtgtgggagggggttgtccagcctccagcagcatggggagggccttggtcagcctctgggtgccagcagggcaggggcggagtcctggggaatgaaggttttatagggctcctgggggaggctccccagccccaagcttaccacctgcacccggagagctgtgtcaccatgtgggtcccggttgtcttcctcaccctgtccgtgacgtggattggtgagaggggccatggttggggggatgcaggagagggagccagccctgactgtcaagctgaggctctttcccccccaacccagcaccccagcccagacagggagctgggctcttttctgtctctcccagccccacttcaagcccatacccccagtcccctccatattgcaacagtcctcactcccacaccaggtccccgctccctcccacttaccccagaactttcttcccatttgcccagccagctccctgctcccagctgctttactaaaggggaagttcctgggcatctccgtgtttctctttgtggggctcaaaacctccaaggacctctctcaatgccattggttccttggaccgtatcactggtccatctcctgagcccctcaatcctatcacagtctactgacttttcccattcagctgtgagtgtccaaccctatcccagagaccttgatgcttggcctcccaatcttgccctaggatacccagatgccaaccagacacctccttctttcctagccaggctatctggcctgagacaacaaatgggtccctcagtctggcaatgggactctgagaactcctcattccctgactcttagccccagactcttcattcagtggcccacattttccttaggaaaaacatgagcatccccagccacaactgccagctctctgagtccccaaatctgcatccttttcaaaacctaaaaacaaaaagaaaaacaaataaaacaaaaccaactcagaccagaactgttttctcaacctgggacttcctaaactttccaaaaccttcctcttccagcaactgaacctcgccataaggcacttatccctggttcctagcaccccttatcccctcagaatccacaacttgtaccaagtttcccttctcccagtccaagaccccaaatcaccacaaaggacccaatccccagactcaagatatggtctgggcgctgtcttgtgtctcctaccctgatccctgggttcaactctgctcccagagcatgaagcctctccaccagcaccagccaccaacctgcaaacctagggaagattgacagaattcccagcctttcccagctccccctgcccatgtcccaggactcccagccttggttctctgcccccgtgtcttttcaaacccacatcctaaatccatctcctatccgagtcccccagttccccctgtcaaccctgattcccctgatctagcaccccctctgcaggcgctgcgcccctcatcctgtctcggattgtgggaggctgggagtgcgagaagcattcccaaccctggcaggtgcttgtggcctctcgtggcagggcagtctgcggcggtgttctggtgcacccccagtgggtcctcacagctgcccactgcatcaggaagtgagtaggggcctggggtctggggagcaggtgtctgtgtcccagaggaataacagctgggcattttccccaggataacctctaaggccagccttgggactgggggagagagggaaagttctggttcaggtcacatggggaggcagggttggggctggaccaccctccccatggctgcctgggtctccatctgtgtccctctatgtctctttgtgtcgctttcattatgtctcttggtaactggcttcggttgtgtctctccgtgtgactattttgttctctctctccctctcttctctgtcttcagtctccatatctccccctctctctgtccttctctggtccctctctagccagtgtgtctcaccctgtatctctctgccaggctctgtctctcggtctctgtctcacctgtgccttctccctactgaacacacgcacgggatgggcctgggggaccctgagaaaaggaagggctttggctgggcgcggtggctcacacctgtaatcccagcactttgggaggccaaggcaggtagatcacctgaggtcaggagttcgagaccagcctggccaactggtgaaaccccatctctactaaaaatacaaaaaattagccaggcgtggtggcgcatgcctgtagtcccagctactcaggagctgagggaggagaattgcattgaacctggaggttgaggttgcagtgagccgagaccgtgccactgcactccagcctgggtgacagagtgagactccgcctcaaaaaaaaaaaaaaaaaaaaaaaaaaaaaagaaaagaaaagaaaagaaaaggaagtgttttatccctgatgtgtgtgggtatgagggtatgagagggcccctctcactccattccttctccaggacatccctccactcttgggagacacagagaagggctggttccagctggagctgggaggggcaattgagggaggaggaaggagaagggggaaggaaaacagggtatgggggaaaggaccctggggagcgaagtggaggatacaaccttgggcctgcaggcaggctacctacccacttggaaacccacgccaaagccgcatctacagctgagccactctgaggcctcccctccccggcggtccccactcagctccaaagtctctctcccttttctctcccacactttatcatcccccggattcctctctacttggttctcattcttcctttgacttcctgcttccctttctcattcatctgtttctcactttctgcctggttttgttcttctctctctctttctctggcccatgtctgtttctctatgtttctgtcttttctttctcatcctgtgtattttcggctcaccttgtttgtcactgttctcccctctgccctttcattctctctgcccttttaccctcttccttttcccttggttctctcagttctgtatctgcccttcaccctctcacactgctgtttcccaactcgttgtctgtattttggcctgaactgtgtcttcccaaccctgtgttttctcactgtttctttttctcttttggagcctcctccttgctcctctgtcccttctctctttccttatcatcctcgctcctcattcctgcgtctgcttcctccccagcaaaagcgtgatcttgctgggtcggcacagcctgtttcatcctgaagacacaggccaggtatttcaggtcagccacagcttcccacacccgctctacgatatgagcctcctgaagaatcgattcctcaggccaggtgatgactccagccacgacctcatgctgctccgcctgtcagagcctgccgagctcacggatgctgtgaaggtcatggacctgcccacccaggagccagcactggggaccacctgctacgcctcaggctggggcagcattgaaccagaggagtgtacgcctgggccagatggtgcagccgggagcccagatgcctgggtctgagggaggaggggacaggactcctgggtctgagggaggagggccaaggaaccaggtggggtccagcccacaacagtgtttttgcctggcccgtagtcttgaccccaaagaaacttcagtgtgtggacctccatgttatttccaatgacgtgtgtgcgcaagttcaccctcagaaggtgaccaagttcatgctgtgtgctggacgctggacagggggcaaaagcacctgctcggtgagtcatccctactcccaagatcttgagggaaaggtgagtgggaccttaattctgggctggggtctagaagccaacaaggcgtctgcctcccctgctccccagctgtagccatgccacctccccgtgtctcatctcattccctccttccctcttctttgactccctcaaggcaataggttattcttacagcacaactcatctgttcctgcgttcagcacacggttactaggcacctgctatgcacccagcactgccctagagcctgggacatagcagtgaacagacagagagcagcccctcccttctgtagcccccaagccagtgaggggcacaggcaggaacagggaccacaacacagaaaagctggagggtgtcaggaggtgatcaggctctcggggagggagaaggggtggggagtgtgactgggaggagacatcctgcagaaggtgggagtgagcaaacacctgcgcaggggaggggagggcctgcggcacctgggggagcagagggaacagcatctggccaggcctgggaggaggggcctagagggcgtcaggagcagagaggaggttgcctggctggagtgaaggatcggggcagggtgcgagagggaacaaaggacccctcctgcagggcctcacctgggccacaggaggacactgcttttcctctgaggagtcaggaactgtggatggtgctggacagaagcaggacagggcctggctcaggtgtccagaggctgcgctggcctcctatgggatcagactgcagggagggagggcagcagggatgtggagggagtgatgatggggctgacctgggggtggctccaggcattgtccccacctgggcccttacccagcctccctcacaggctcctggccctcagtctctcccctccactccattctccacctacccacagtgggtcattctgatcaccgaactgaccatgccagccctgccgatggtcctccatggctccctagtgccctggagaggaggtgtctagtcagagagtagtcctggaaggtggcctctgtgaggagccacggggacagcatcctgcagatgg
tcctggcccttgtcccaccgacctgtctacaaggactgtcctcgtggaccctcccctctgcacaggagctggaccctgaagtcccttcctaccggccaggactggagcccctacccctctgttggaatccctgcccaccttcttctggaagtcggctctggagacatttctctcttcttccaaagctgggaactgctatctgttatctgcctgtccaggtctgaaagataggattgcccaggcagaaactgggactgacctatctcactctctccctgcttttacccttagggtgattctgggggcccacttgtctgtaatggtgtgcttcaaggtatcacgtcatggggcagtgaaccatgtgccctgcccgaaaggccttccctgtacaccaaggtggtgcattaccggaagtggatcaaggacaccatcgtggccaacccctgagcacccctatcaagtccctattgtagtaaacttggaaccttggaaatgaccaggccaagactcaagcctccccagttctactgacctttgtccttaggtgtgaggtccagggttgctaggaaaagaaatcagcagacacaggtgtagaccagagtgtttcttaaatggtgtaattttgtcctctctgtgtcctggggaatactggccatgcctggagacatatcactcaatttctctgaggacacagttaggatggggtgtctgtgttatttgtgggatacagagatgaaagaggggtgggatcc(配列番号:26;GenBankアクセス番号X14810)。もう1つの実施態様において、KLK3蛋白質は配列番号:26の残基401ないし446、1688ないし1847、3477ないし3763、3907ないし4043、5413ないし5568、またはその組合せによってコードされる。もう1つの実施態様において、KLK3蛋白質は配列番号:26のホモログによってコードされる。もう1つの実施態様において、KLK3蛋白質は配列番号:26の変種によってコードされる。もう1つの実施態様において、KLK3蛋白質は配列番号:26のイソ形態によってコードされる。もう1つの実施態様において、KLK3蛋白質は配列番号:26の断片によってコードされる。各可能性は本発明の別の実施態様を表わす。
MWVPVVFLTLSVTWIGAAPLILSRIVGGWECEKHSQPWQVLVASRGRAVCGGVLVHPQWVLTAAHCIRNKSVILLGRHSLFHPEDTGQVFQVSHSFPHPLYDMSLLKNRFLRPGDDSSHDLMLLRLSEPAELTDAVKVMDLPTQEPALGTTCYASGWGSIEPEEFLTPKKLQCVDLHVISNDVCAQVHPQKVTKFMLCAGRWTGGKSTCSWVILITELTMPALPMVLHGSLVPWRGGV(配列番号:27;GenBankアクセス番号NM_001030047)。もう1つの実施態様において、KLK3蛋白質は配列番号:27のホモログである。もう1つの実施態様において、KLK3蛋白質は配列番号:27の変種である。もう1つの実施態様において、KLK3蛋白質は配列番号:27のイソ形態である。もう1つの実施態様において、KLK3蛋白質は配列番号:27の断片である。各可能性は本発明の別の実施態様を表わす。
agccccaagcttaccacctgcacccggagagctgtgtcaccatgtgggtcccggttgtcttcctcaccctgtccgtgacgtggattggtgctgcacccctcatcctgtctcggattgtgggaggctgggagtgcgagaagcattcccaaccctggcaggtgcttgtggcctctcgtggcagggcagtctgcggcggtgttctggtgcacccccagtgggtcctcacagctgcccactgcatcaggaacaaaagcgtgatcttgctgggtcggcacagcctgtttcatcctgaagacacaggccaggtatttcaggtcagccacagcttcccacacccgctctacgatatgagcctcctgaagaatcgattcctcaggccaggtgatgactccagccacgacctcatgctgctccgcctgtcagagcctgccgagctcacggatgctgtgaaggtcatggacctgcccacccaggagccagcactggggaccacctgctacgcctcaggctggggcagcattgaaccagaggagttcttgaccccaaagaaacttcagtgtgtggacctccatgttatttccaatgacgtgtgtgcgcaagttcaccctcagaaggtgaccaagttcatgctgtgtgctggacgctggacagggggcaaaagcacctgctcgtgggtcattctgatcaccgaactgaccatgccagccctgccgatggtcctccatggctccctagtgccctggagaggaggtgtctagtcagagagtagtcctggaaggtggcctctgtgaggagccacggggacagcatcctgcagatggtcctggcccttgtcccaccgacctgtctacaaggactgtcctcgtggaccctcccctctgcacaggagctggaccctgaagtcccttccccaccggccaggactggagcccctacccctctgttggaatccctgcccaccttcttctggaagtcggctctggagacatttctctcttcttccaaagctgggaactgctatctgttatctgcctgtccaggtctgaaagataggattgcccaggcagaaactgggactgacctatctcactctctccctgcttttacccttagggtgattctgggggcccacttgtctgtaatggtgtgcttcaaggtatcacgtcatggggcagtgaaccatgtgccctgcccgaaaggccttccctgtacaccaaggtggtgcattaccggaagtggatcaaggacaccatcgtggccaacccctgagcacccctatcaaccccctattgtagtaaacttggaaccttggaaatgaccaggccaagactcaagcctccccagttctactgacctttgtccttaggtgtgaggtccagggttgctaggaaaagaaatcagcagacacaggtgtagaccagagtgtttcttaaatggtgtaattttgtcctctctgtgtcctggggaatactggccatgcctggagacatatcactcaatttctctgaggacacagataggatggggtgtctgtgttatttgtggggtacagagatgaaagaggggtgggatccacactgagagagtggagagtgacatgtgctggacactgtccatgaagcactgagcagaagctggaggcacaacgcaccagacactcacagcaaggatggagctgaaaacataacccactctgtcctggaggcactgggaagcctagagaaggctgtgagccaaggagggagggtcttcctttggcatgggatggggatgaagtaaggagagggactggaccccctggaagctgattcactatggggggaggtgtattgaagtcctccagacaaccctcagatttgatgatttcctagtagaactcacagaaataaagagctgttatactgtg(配列番号:28;GenBankアクセス番号NM 001030047)。もう1つの実施態様において、KLK3蛋白質は配列番号:28の残基42ないし758によってコードされる。もう1つの実施態様において、KLK3蛋白質は配列番号:28のホモログによってコードされる。もう1つの実施態様において、KLK3蛋白質は配列番号:28の変種によってコードされる。もう1つの実施態様において、KLK3蛋白質は配列番号:28のイソ形態によってコードされる。もう1つの実施態様において、KLK3蛋白質は配列番号:28の断片によってコードされる。各可能性は本発明の別の実施態様を表わす。
MWVPVVFLTLSVTWIGAAPLILSRIVGGWECEKHSQPWQVLVASRGRAVCGGVLVHPQWVLTAAHCIRK(配列番号:29;GenBankアクセス番号NM_001030050)。もう1つの実施態様において、KLK3蛋白質は配列番号:29のホモログである。もう1つの実施態様において、KLK3蛋白質は配列番号:29の変種である。もう1つの実施態様において、KLK3蛋白質の配列は配列番号:29を含む。もう1つの実施態様において、KLK3蛋白質は配列番号:29のイソ形態である。もう1つの実施態様において、KLK3蛋白質は配列番号:29の断片である。各可能性は本発明の別の実施態様を表わす。
MWVPVVFLTLSVTWIGAAPLILSRIVGGWECEKHSQPWQVLVASRGRAVCGGVLVHPQWVLTAAHCIRNKSVILLGRHSLFHPEDTGQVFQVSHSFPHPLYDMSLLKNRFLRPGDDSSIEPEEFLTPKKLQCVDLHVISNDVCAQVHPQKVTKFMLCAGRWTGGKSTCSGDSGGPLVCNGVLQGITSWGSEPCALPERPSLYTKVVHYRKWIKDTIVANP(配列番号:30;GenBankアクセス番号NM_001030049)。もう1つの実施態様において、KLK3蛋白質は配列番号:30のホモログである。もう1つの実施態様において、KLK3蛋白質は配列番号:30の変種である。もう1つの実施態様において、KLK3蛋白質は配列番号:30のイソ形態である。もう1つの実施態様において、KLK3蛋白質は配列番号:30の断片である。各可能性は本発明の別の実施態様を表わす。
MWVPVVFLTLSVTWIGAAPLILSRIVGGWECEKHSQPWQVLVASRGRAVCGGVLVHPQWVLTAAHCIRKPGDDSSHDLMLLRLSEPAELTDAVKVMDLPTQEPALGTTCYASGWGSIEPEEFLTPKKLQCVDLHVISNDVCAQVHPQKVTKFMLCAGRWTGGKSTCSGDSGGPLVCNGVLQGITSWGSEPCALPERPSLYTKVVHYRKWIKDTIVANP(配列番号:31;GenBankアクセス番号NM_001030048)。もう1つの実施態様において、KLK3蛋白質は配列番号:31のホモログである。もう1つの実施態様において、KLK3蛋白質は配列番号:31の変種である。もう1つの実施態様において、KLK3蛋白質は配列番号:31のイソ形態である。もう1つの実施態様において、KLK3蛋白質は配列番号:31の断片である。各可能性は本発明の別の実施態様を表わす。
MWVPVVFLTLSVTWIGAAPLILSRIVGGWECEKHSQPWQVLVASRGRAVCGGVLVHPQWVLTAAHCIRNKSVILLGRHSLFHPEDTGQVFQVSHSFPHPLYDMSLLKNRFLRPGDDSSHDLMLLRLSEPAELTDAVKVMDLPTQEPALGTTCYASGWGSIEPEEFLTPKKLQCVDLHVISNDVCAQVHPQKVTKFMLCAGRWTGGKSTCSGDSGGPLVCNGVLQGITSWGSEPCALPERPSLYTKVVHYRKWIKDTIVANP(配列番号:32;GenBankアクセス番号NM_001648)。もう1つの実施態様において、KLK3蛋白質は配列番号:32のホモログである。もう1つの実施態様において、KLK3蛋白質は配列番号:32の変種である。もう1つの実施態様において、KLK3蛋白質は配列番号:32のイソ形態である。もう1つの実施態様において、KLK3蛋白質は配列番号:32の断片である。各可能性は本発明の別の実施態様を表わす。
agccccaagcttaccacctgcacccggagagctgtgtcaccatgtgggtcccggttgtcttcctcaccctgtccgtgacgtggattggtgctgcacccctcatcctgtctcggattgtgggaggctgggagtgcgagaagcattcccaaccctggcaggtgcttgtggcctctcgtggcagggcagtctgcggcggtgttctggtgcacccccagtgggtcctcacagctgcccactgcatcaggaacaaaagcgtgatcttgctgggtcggcacagcctgtttcatcctgaagacacaggccaggtatttcaggtcagccacagcttcccacacccgctctacgatatgagcctcctgaagaatcgattcctcaggccaggtgatgactccagccacgacctcatgctgctccgcctgtcagagcctgccgagctcacggatgctgtgaaggtcatggacctgcccacccaggagccagcactggggaccacctgctacgcctcaggctggggcagcattgaaccagaggagttcttgaccccaaagaaacttcagtgtgtggacctccatgttatttccaatgacgtgtgtgcgcaagttcaccctcagaaggtgaccaagttcatgctgtgtgctggacgctggacagggggcaaaagcacctgctcgggtgattctgggggcccacttgtctgtaatggtgtgcttcaaggtatcacgtcatggggcagtgaaccatgtgccctgcccgaaaggccttccctgtacaccaaggtggtgcattaccggaagtggatcaaggacaccatcgtggccaacccctgagcacccctatcaaccccctattgtagtaaacttggaaccttggaaatgaccaggccaagactcaagcctccccagttctactgacctttgtccttaggtgtgaggtccagggttgctaggaaaagaaatcagcagacacaggtgtagaccagagtgtttcttaaatggtgtaattttgtcctctctgtgtcctggggaatactggccatgcctggagacatatcactcaatttctctgaggacacagataggatggggtgtctgtgttatttgtggggtacagagatgaaagaggggtgggatccacactgagagagtggagagtgacatgtgctggacactgtccatgaagcactgagcagaagctggaggcacaacgcaccagacactcacagcaaggatggagctgaaaacataacccactctgtcctggaggcactgggaagcctagagaaggctgtgagccaaggagggagggtcttcctttggcatgggatggggatgaagtaaggagagggactggaccccctggaagctgattcactatggggggaggtgtattgaagtcctccagacaaccctcagatttgatgatttcctagtagaactcacagaaataaagagctgttatactgtg(配列番号:33;GenBankアクセス番号NM_001648)。もう1つの実施態様において、KLK3蛋白質は配列番号:33の残基42ないし827によってコードされる。もう1つの実施態様において、KLK3蛋白質は配列番号:33のホモログによってコードされる。もう1つの実施態様において、KLK3蛋白質は配列番号:33の変種によってコードされる。もう1つの実施態様において、KLK3蛋白質は配列番号:33のイソ形態によってコードされる。もう1つの実施態様において、KLK3蛋白質は配列番号:33の断片によってコードされる。各可能性は本発明の別の実施態様を表わす。
MWVPVVFLTLSVTWIGAAPLILSRIVGGWECEKHSQPWQVLVASRGRAVCGGVLVHPQWVLTAAHCIRNKSVILLGRHSLFHPEDTGQVFQVSHSFPHPLYDMSLLKNRFLRPGDDSSHDLMLLRLSEPAELTDAVKVMDLPTQEPALGTTCYASGWGSIEPEEFLTPKKLQCVDLHVISNDVCAQVHPQKVTKFMLCAGRWTGGKSTCSGDSGGPLVCNGVLQGITSWGSEPCALPERPSLYTKVVHYRKWIKDTIVANP(配列番号:34;GenBankアクセス番号BC056665)。もう1つの実施態様において、KLK3蛋白質は配列番号:34のホモログである。もう1つの実施態様において、KLK3蛋白質は配列番号:34の変種である。もう1つの実施態様において、KLK3蛋白質は配列番号:34のイソ形態である。もう1つの実施態様において、KLK3蛋白質は配列番号:34の断片である。各可能性は本発明の別の実施態様を表わす。
gggggagccccaagcttaccacctgcacccggagagctgtgtcaccatgtgggtcccggttgtcttcctcaccctgtccgtgacgtggattggtgctgcacccctcatcctgtctcggattgtgggaggctgggagtgcgagaagcattcccaaccctggcaggtgcttgtggcctctcgtggcagggcagtctgcggcggtgttctggtgcacccccagtgggtcctcacagctgcccactgcatcaggaacaaaagcgtgatcttgctgggtcggcacagcctgtttcatcctgaagacacaggccaggtatttcaggtcagccacagcttcccacacccgctctacgatatgagcctcctgaagaatcgattcctcaggccaggtgatgactccagccacgacctcatgctgctccgcctgtcagagcctgccgagctcacggatgctgtgaaggtcatggacctgcccacccaggagccagcactggggaccacctgctacgcctcaggctggggcagcattgaaccagaggagttcttgaccccaaagaaacttcagtgtgtggacctccatgttatttccaatgacgtgtgtgcgcaagttcaccctcagaaggtgaccaagttcatgctgtgtgctggacgctggacagggggcaaaagcacctgctcgggtgattctgggggcccacttgtctgtaatggtgtgcttcaaggtatcacgtcatggggcagtgaaccatgtgccctgcccgaaaggccttccctgtacaccaaggtggtgcattaccggaagtggatcaaggacaccatcgtggccaacccctgagcacccctatcaactccctattgtagtaaacttggaaccttggaaatgaccaggccaagactcaggcctccccagttctactgacctttgtccttaggtgtgaggtccagggttgctaggaaaagaaatcagcagacacaggtgtagaccagagtgtttcttaaatggtgtaattttgtcctctctgtgtcctggggaatactggccatgcctggagacatatcactcaatttctctgaggacacagataggatggggtgtctgtgttatttgtggggtacagagatgaaagaggggtgggatccacactgagagagtggagagtgacatgtgctggacactgtccatgaagcactgagcagaagctggaggcacaacgcaccagacactcacagcaaggatggagctgaaaacataacccactctgtcctggaggcactgggaagcctagagaaggctgtgagccaaggagggagggtcttcctttggcatgggatggggatgaagtagggagagggactggaccccctggaagctgattcactatggggggaggtgtattgaagtcctccagacaaccctcagatttgatgatttcctagtagaactcacagaaataaagagctgttatactgcgaaaaaaaaaaaaaaaaaaaaaaaaaa(配列番号:35;GenBankアクセス番号BC056665)。もう1つの実施態様において、KLK3蛋白質は配列番号:35の残基47ないし832によってコードされる。もう1つの実施態様において、KLK3蛋白質は配列番号:35のホモログによってコードされる。もう1つの実施態様において、KLK3蛋白質は配列番号:35の変種によってコードされる。もう1つの実施態様において、KLK3蛋白質は配列番号:35のイソ形態によってコードされる。もう1つの実施態様において、KLK3蛋白質は配列番号:35の断片によってコードされる。各可能性は本発明の別の実施態様を表わす。
MWVPVVFLTLSVTWIGAAPLILSRIVGGWECEKHSQPWQVLVASRGRAVCGGVLVHPQWVLTAAHCIRNKSVILLGRHSLFHPEDTGQVFQVSHSFPHPLYDMSLLKNRFLRPGDDSSIEPEEFLTPKKLQCVDLHVISNDVCAQVHPQKVTKFMLCAGRWTGGKSTCSGDSGGPLVCNGVLQGITSWGSEPCALPERPSLYTKVVHYRKWIKDTIVA(配列番号:36;GenBankアクセス番号AJ459782)。もう1つの実施態様において、KLK3蛋白質は配列番号:36のホモログである。もう1つの実施態様において、KLK3蛋白質は配列番号:36の変種である。もう1つの実施態様において、KLK3蛋白質は配列番号:36のイソ形態である。もう1つの実施態様において、KLK3蛋白質は配列番号:36の断片である。各可能性は本発明の別の実施態様を表わす。
MWVPVVFLTLSVTWIGAAPLILSRIVGGWECEKHSQPWQVLVASRGRAVCGGVLVHPQWVLTAAHCIRNKSVILLGRHSLFHPEDTGQVFQVSHSFPHPLYDMSLLKNRFLRPGDDSSHDLMLLRLSEPAELTDAVKVMDLPTQEPALGTTCYASGWGSIEPEEFLTPKKLQCVDLHVISNDVCAQVHPQKVTKFMLCAGRWTGGKSTCSVSHPYSQDLEGKGEWGP(配列番号:37;GenBankアクセス番号AJ512346)。もう1つの実施態様において、KLK3蛋白質は配列番号:37のホモログである。もう1つの実施態様において、KLK3蛋白質は配列番号:37の変種である。もう1つの実施態様において、KLK3蛋白質は配列番号:37のイソ形態である。もう1つの実施態様において、KLK3蛋白質の配列は配列番号:37を含む。もう1つの実施態様において、KLK3蛋白質は配列番号:37の断片である。各可能性は本発明の別の実施態様を表わす。
MWVPVVFLTLSVTWIGERGHGWGDAGEGASPDCQAEALSPPTQHPSPDRELGSFLSLPAPLQAHTPSPSILQQSSLPHQVPAPSHLPQNFLPIAQPAPCSQLLY(配列番号:38;GenBankアクセス番号AJ459784)。もう1つの実施態様において、KLK3蛋白質は配列番号:38のホモログである。もう1つの実施態様において、KLK3蛋白質は配列番号:38の変種である。もう1つの実施態様において、KLK3蛋白質の配列は配列番号:38を含む。もう1つの実施態様において、KLK3蛋白質は配列番号:38のイソ形態である。もう1つの実施態様において、KLK3蛋白質は配列番号:38の断片である。各可能性は本発明の別の実施態様を表わす。
MWVPVVFLTLSVTWIGAAPLILSRIVGGWECEKHSQPWQVLVASRGRAVCGGVLVHPQWVLTAAHCIRNKSVILLGRHSLFHPEDTGQVFQVSHSFPHPLYDMSLLKNRFLRPGDDSSHDLMLLRLSEPAELTDAVKVMDLPTQEPALGTTCYASGWGSIEPEEFLTPKKLQCVDLHVISNDVCAQVHPQKVTKFMLCAGRWTGGKSTCSGDSGGPLVCNGVLQGITSWGSEPCALPERPSLYTKVVHYRKWIKDTIVANP(配列番号:39;GenBankアクセス番号AJ459783)。もう1つの実施態様において、KLK3蛋白質は配列番号:39のホモログである。もう1つの実施態様において、KLK3蛋白質は配列番号:39の変種である。もう1つの実施態様において、KLK3蛋白質は配列番号:39のイソ形態である。もう1つの実施態様において、KLK3蛋白質は配列番号:39の断片である。各可能性は本発明の別の実施態様を表わす。
aagtttcccttctcccagtccaagaccccaaatcaccacaaaggacccaatccccagactcaagatatggtctgggcgctgtcttgtgtctcctaccctgatccctgggttcaactctgctcccagagcatgaagcctctccaccagcaccagccaccaacctgcaaacctagggaagattgacagaattcccagcctttcccagctccccctgcccatgtcccaggactcccagccttggttctctgcccccgtgtcttttcaaacccacatcctaaatccatctcctatccgagtcccccagttcctcctgtcaaccctgattcccctgatctagcaccccctctgcaggtgctgcacccctcatcctgtctcggattgtgggaggctgggagtgcgagaagcattcccaaccctggcaggtgcttgtagcctctcgtggcagggcagtctgcggcggtgttctggtgcacccccagtgggtcctcacagctacccactgcatcaggaacaaaagcgtgatcttgctgggtcggcacagcctgtttcatcctgaagacacaggccaggtatttcaggtcagccacagcttcccacacccgctctacgatatgagcctcctgaagaatcgattcctcaggccaggtgatgactccagccacgacctcatgctgctccgcctgtcagagcctgccgagctcacggatgctatgaaggtcatggacctgcccacccaggagccagcactggggaccacctgctacgcctcaggctggggcagcattgaaccagaggagttcttgaccccaaagaaacttcagtgtgtggacctccatgttatttccaatgacgtgtgtgcgcaagttcaccctcagaaggtgaccaagttcatgctgtgtgctggacgctggacagggggcaaaagcacctgctcgggtgattctgggggcccacttgtctgtaatggtgtgcttcaaggtatcacgtcatggggcagtgaaccatgtgccctgcccgaaaggccttccctgtacaccaaggtggtgcattaccggaagtggatcaaggacaccatcgtggccaacccctgagcacccctatcaactccctattgtagtaaacttggaaccttggaaatgaccaggccaagactcaggcctccccagttctactgacctttgtccttaggtgtgaggtccagggttgctaggaaaagaaatcagcagacacaggtgtagaccagagtgtttcttaaatggtgtaattttgtcctctctgtgtcctggggaatactggccatgcctggagacatatcactcaatttctctgaggacacagataggatggggtgtctgtgttatttgtggggtacagagatgaaagaggggtgggatccacactgagagagtggagagtgacatgtgctggacactgtccatgaagcactgagcagaagctggaggcacaacgcaccagacactcacagcaaggatggagctgaaaacataacccactctgtcctggaggcactgggaagcctagagaaggctgtgaaccaaggagggagggtcttcctttggcatgggatggggatgaagtaaggagagggactgaccccctggaagctgattcactatggggggaggtgtattgaagtcctccagacaaccctcagatttgatgatttcctagtagaactcacagaaataaagagctgttatactgtgaa(配列番号:40;GenBankアクセス番号X07730)。もう1つの実施態様において、KLK3蛋白質は配列番号:40の残基67ないし1088によってコードされる。もう1つの実施態様において、KLK3蛋白質は配列番号:40のホモログによってコードされる。もう1つの実施態様において、KLK3蛋白質は配列番号:40の変種によってコードされる。もう1つの実施態様において、KLK3蛋白質は配列番号:40のイソ形態によってコードされる。もう1つの実施態様において、KLK3蛋白質は配列番号:40の断片によってコードされる。各可能性は本発明の別の実施態様を表わす。
MKAVLLALLM AGLALQPGTA LLCYSCKAQV SNEDCLQVEN CTQLGEQCWT ARIRAVGLLT VISKGCSLNC VDDSQDYYVG KKNITCCDTD LCNASGAHAL QPAAAILALL PALGLLLWGP GQL(配列番号:63)。
agggagaggcagtgaccatgaaggctgtgctgcttgccctgttgatggcaggcttggccctgcagccaggcactgccctgctgtgctactcctgcaaagcccaggtgagcaacgaggactgcctgcaggtggagaactgcacccagctgggggagcagtgctggaccgcgcgcatccgcgcagttggcctcctgaccgtcatcagcaaaggctgcagcttgaactgcgtggatgactcacaggactactacgtgggcaagaagaacatcacgtgctgtgacaccgacttgtgcaacgccagcggggcccatgccctgcagccggctgccgccatccttgcgctgctccctgcactcggcctgctgctctggggacccggccagctataggctctggggggccccgctgcagcccacactgggtgtggtgccccaggcctttgtgccactcctcacagaacctggcccagtgggagcctgtcctggttcctgaggcacatcctaacgcaagtttgaccatgtatgtttgcaccccttttccccnaaccctgaccttcccatgggccttttccaggattccnaccnggcagatcagttttagtganacanatccgcntgcagatggcccctccaaccntttntgttgntgtttccatggcccagcattttccacccttaaccctgtgttcaggcacttnttcccccaggaagccttccctgcccaccccatttatgaattgagccaggtttggtccgtggtgtcccccgcacccagcaggggacaggcaatcaggagggcccagtaaaggctgagatgaagtggactgagtagaactggaggacaagagttgacgtgagttcctgggagtttccagagatggggcctggaggcctggaggaaggggccaggcctcacatttgtggggntcccgaatggcagcctgagcacagcgtaggcccttaataaacacctgttggataagccaaaaaa(配列番号:64)。
MWNLLHETDSAVATARRPRWLCAGALVLAGGFFLLGFLFGWFIKSSNEATNITPKHNMKAFLDELKAENIKKFLYNFTQIPHLAGTEQNFQLAKQIQSQWKEFGLDSVELAHYDVLLSYPNKTHPNYISIINEDGNEIFNTSLFEPPPPGYENVSDIVPPFSAFSPQGMPEGDLVYVNYARTEDFFKLERDMKINCSGKIVIARYGKVFRGNKVKNAQLAGAKGVILYSDPADYFAPGVKSYPDGWNLPGGGVQRGNILNLNGAGDPLTPGYPANEYAYRRGIAEAVGLPSIPVHPIGYYDAQKLLEKMGGSAPPDSSWRGSLKVPYNVGPGFTGNFSTQKVKMHIHSTNEVTRIYNVIGTLRGAVEPDRYVILGGHRDSWVFGGIDPQSGAAVVHEIVRSFGTLKKEGWRPRRTILFASWDAEEFGLLGSTEWAEENSRLLQERGVAYINADSSIEGNYTLRVDCTPLMYSLVHNLTKELKSPDEGFEGKSLYESWTKKSPSPEFSGMPRISKLGSGNDFEVFFQRLGIASGRARYTKNWETNKFSGYPLYHSVYETYELVEKFYDPMFKYHLTVAQVRGGMVFELANSIVLPFDCRDYAVVLRKYADKIYSISMKHPQEMKTYSVSFDSLFSAVKNFTEIASKFSERLQDFDKSKHVIYAPSSHNKYAGESFPGIYDALFDIESKVDPSKAWGEVKRQIYVAAFTVQAAAETLSEVA(配列番号:41;GenBankアクセス番号:BC025672)。もう1つの実施態様において、FOLH1蛋白質は配列番号:41のホモログである。もう1つの実施態様において、FOLH1蛋白質は配列番号:41の変種である。もう1つの実施態様において、FOLH1蛋白質は配列番号:41のイソ形態である。もう1つの実施態様において、FOLH1蛋白質は配列番号:41の断片である。各可能性は本発明の別の実施態様を表わす。
ctggaccccaggtctggagcgaattccagcctgcagggctgataagcgaggcattagtgagattgagagagactttaccccgccgtggtggttggagggcgcgcagtagagcagcagcacaggcgcgggtcccgggaggccggctctgctcgcgccgagatgtggaatctccttcacgaaaccgactcggctgtggccaccgcgcgccgcccgcgctggctgtgcgctggggcgctggtgctggcgggtggcttctttctcctcggcttcctcttcgggtggtttataaaatcctccaatgaagctactaacattactccaaagcataatatgaaagcatttttggatgaattgaaagctgagaacatcaagaagttcttatataattttacacagataccacatttagcaggaacagaacaaaactttcagcttgcaaagcaaattcaatcccagtggaaagaatttggcctggattctgttgagctagcacattatgatgtcctgttgtcctacccaaataagactcatcccaactacatctcaataattaatgaagatggaaatgagattttcaacacatcattatttgaaccacctcctccaggatatgaaaatgtttcggatattgtaccacctttcagtgctttctctcctcaaggaatgccagagggcgatctagtgtatgttaactatgcacgaactgaagacttctttaaattggaacgggacatgaaaatcaattgctctgggaaaattgtaattgccagatatgggaaagttttcagaggaaataaggttaaaaatgcccagctggcaggggccaaaggagtcattctctactccgaccctgctgactactttgctcctggggtgaagtcctatccagatggttggaatcttcctggaggtggtgtccagcgtggaaatatcctaaatctgaatggtgcaggagaccctctcacaccaggttacccagcaaatgaatatgcttataggcgtggaattgcagaggctgttggtcttccaagtattcctgttcatccaattggatactatgatgcacagaagctcctagaaaaaatgggtggctcagcaccaccagatagcagctggagaggaagtctcaaagtgccctacaatgttggacctggctttactggaaacttttctacacaaaaagtcaagatgcacatccactctaccaatgaagtgacaagaatttacaatgtgataggtactctcagaggagcagtggaaccagacagatatgtcattctgggaggtcaccgggactcatgggtgtttggtggtattgaccctcagagtggagcagctgttgttcatgaaattgtgaggagctttggaacactgaaaaaggaagggtggagacctagaagaacaattttgtttgcaagctgggatgcagaagaatttggtcttcttggttctactgagtgggcagaggagaattcaagactccttcaagagcgtggcgtggcttatattaatgctgactcatctatagaaggaaactacactctgagagttgattgtacaccgctgatgtacagcttggtacacaacctaacaaaagagctgaaaagccctgatgaaggctttgaaggcaaatctctttatgaaagttggactaaaaaaagtccttccccagagttcagtggcatgcccaggataagcaaattgggatctggaaatgattttgaggtgttcttccaacgacttggaattgcttcaggcagagcacggtatactaaaaattgggaaacaaacaaattcagcggctatccactgtatcacagtgtctatgaaacatatgagttggtggaaaagttttatgatccaatgtttaaatatcacctcactgtggcccaggttcgaggagggatggtgtttgagctagccaattccatagtgctcccttttgattgtcgagattatgctgtagttttaagaaagtatgctgacaaaatctacagtatttctatgaaacatccacaggaaatgaagacatacagtgtatcatttgattcacttttttctgcagtaaagaattttacagaaattgcttccaagttcagtgagagactccaggactttgacaaaagcaagcatgtcatctatgctccaagcagccacaacaagtatgcaggggagtcattcccaggaatttatgatgctctgtttgatattgaaagcaaagtggacccttccaaggcctggggagaagtgaagagacagatttatgttgcagccttcacagtgcaggcagctgcagagactttgagtgaagtagcctaagaggattctttagagaatccgtattgaatttgtgtggtatgtcactcagaaagaatcgtaatgggtatattgataaattttaaaattggtatatttgaaataaagttgaatattatatataaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa(配列番号:42;GenBankアクセス番号:BC025672)。もう1つの実施態様において、FOLH1蛋白質は配列番号:42の残基160ないし2319によってコードされる。もう1つの実施態様において、FOLH1蛋白質は配列番号:42のホモログによってコードされる。もう1つの実施態様において、FOLH1蛋白質は配列番号:42の変種によってコードされる。もう1つの実施態様において、FOLH1蛋白質は配列番号:42のイソ形態によってコードされる。もう1つの実施態様において、FOLH1蛋白質は配列番号:42の断片によってコードされる。各可能性は本発明の別の実施態様を表わす。
MWNLLHETDSAVATARRPRWLCAGALVLAGGFFLLGFLFGWFIKSSNEATNITPKHNMKAFLDELKAENIKKFLYNFTQIPHLAGTEQNFQLAKQIQSQWKEFGLDSVELAHYDVLLSYPNKTHPNYISIINEDGNEIFNTSLFEPPPPGYENVSDIVPPFSAFSPQGMPEGDLVYVNYARTEDFFKLERDMKINCSGKIVIARYGKVFRGNKVKNAQLAGAKGVILYSDPADYFAPGVKSYPDGWNLPGGGVQRGNILNLNGAGDPLTPGYPANEYAYRRGIAEAVGLPSIPVHPIGYYDAQKLLEKMGGSAPPDSSWRGSLKVPYNVGPGFTGNFSTQKVKMHIHSTNEVTRIYNVIGTLRGAVEPDRYVILGGHRDSWVFGGIDPQSGAAVVHEIVRSFGTLKKEGWRPRRTILFASWDAEEFGLLGSTEWAEENSRLLQERGVAYINADSSIEGNYTLRVDCTPLMYSLVHNLTKELKSPDEGFEGKSLYESWTKKSPSPEFSGMPRISKLGSGNDFEVFFQRLGIASGRARYTKNWETNKFSGYPLYHSVYETYELVEKFYDPMFKYHLTVAQVRGGMVFELANSIVLPFDCRDYAVVLRKYADKIYSISMKHPQEMKTYSVSFDSLFSAVKNFTEIASKFSERLQDFDKSKHVIYAPSSHNKYAGESFPGIYDALFDIESKVDPSKAWGEVKRQIYVAAFTVQAAAETLSEVA(配列番号:43;GenBankアクセス番号:NM_001014986)。もう1つの実施態様において、FOLH1蛋白質は配列番号:43のホモログである。もう1つの実施態様において、FOLH1蛋白質は配列番号:43の変種である。もう1つの実施態様において、FOLH1蛋白質は配列番号:43のイソ形態である。もう1つの実施態様において、FOLH1蛋白質は配列番号:43の断片である。各可能性は本発明の別の実施態様を表わす。
MWNLLHETDSAVATARRPRWLCAGALVLAGGFFLLGFLFGWFIKSSNEATNITPKHNMKAFLDELKAENIKKFLYNFTQIPHLAGTEQNFQLAKQIQSQWKEFGLDSVELAHYDVLLSYPNKTHPNYISIINEDGNEIFNTSLFEPPPPGYENVSDIVPPFSAFSPQGMPEGDLVYVNYARTEDFFKLERDMKINCSGKIVIARYGKVFRGNKVKNAQLAGAKGVILYSDPADYFAPGVKSYPDGWNLPGGGVQRGNILNLNGAGDPLTPGYPANEYAYRRGIAEAVGLPSIPVHPIGYYDAQKLLEKMGGSAPPDSSWRGSLKVPYNVGPGFTGNFSTQKVKMHIHSTNEVTRIYNVIGTLRGAVEPDRYVILGGHRDSWVFGGIDPQSGAAVVHEIVRSFGTLKKEGWRPRRTILFASWDAEEFGLLGSTEWAEENSRLLQERGVAYINADSSIEGNYTLRVDCTPLMYSLVHNLTKELKSPDEGFEGKSLYESWTKKSPSPEFSGMPRISKLGSGNDFEVFFQRLGIASGRARYTKNWETNKFSGYPLYHSVYETYELVEKFYDPMFKYHLTVAQVRGGMVFELANSIVLPFDCRDYAVVLRKYADKIYSISMKHPQEMKTYSVSFDSLFSAVKNFTEIASKFSERLQDFDKSNPIVLRMMNDQLMFLERAFIDPLGLPDRPFYRHVIYAPSSHNKYAGESFPGIYDALFDIESKVDPSKAWGEVKRQIYVAAFTVQAAAETLSEVA(配列番号:44;GenBankアクセス番号:NM_004476)。もう1つの実施態様において、FOLH1蛋白質は配列番号:44のホモログである。もう1つの実施態様において、FOLH1蛋白質は配列番号:44の変種である。もう1つの実施態様において、FOLH1蛋白質は配列番号:44のイソ形態である。もう1つの実施態様において、FOLH1蛋白質は配列番号:44の断片である。各可能性は本発明の別の実施態様を表わす。
IPHLAGTEQNFQLAKQIQSQWKEFGLDSVELAHYDVLLSYPNKTHPNYISIINEDGNEIFNTSLFEPPPPGYENVSDIVPPFSAFSPQGMPEGDLVYVNYARTEDFFKLERDMKINCSGKIVIARYGKVFRGNKVKNAQLAGAKGVILYSDPADYFAPGVKSYPDGWNLPGGGVQRGNILNLNGAGDPLTPGYPANEYAYRRGIAEAVGLPSIPVHPIGYYDAQKLLEKMGGSAPPDSSWRGSLKVPYNVGPGFTGNFSTQKVKMHIHSTNEVTRIYNVIGTLRGAVEPDRYVILGGHRDSWVFGGIDPQSGAAVVHEIVRSFGTLKKEGWRPRRTILFASWDAEEFGLLGSTEWAEENSRLLQERGVAYINADSSIEGNYTLRVDCTPLMYSLVHNLTKELKSPDEGFEGKSLYESWTKKSPSPEFSGMPRISKLGSGNDFEVFFQRLGIASGRARYTKNWETNKFSGYPLYHSVYETYELVEKFYDPMFKYHLTVAQVRGGMVFELANSIVLPFDCRDYAVVLRKYADKIYSISMKHPQEMKTYSVSFDSLFSAVKNFTEIASKFSERLQDFDKSNPIVLRMMNDQLMFLERAFIDPLGLPDRPFYRHVIYAPSSHNKYAGESFPGIYDALFDIESKVDPSKAWGEVKRQIYVAAFTVQAAAETLSEVA(配列番号:45;GenBankアクセス番号:BC108719)。もう1つの実施態様において、FOLH1蛋白質は配列番号:45のホモログである。もう1つの実施態様において、FOLH1蛋白質は配列番号:45の変種である。もう1つの実施態様において、FOLH1蛋白質は配列番号:45のイソ形態である。もう1つの実施態様において、FOLH1蛋白質は配列番号:45の断片である。各可能性は本発明の別の実施態様を表わす。
MKKIMLVFITLILVSLPIAQQTEAKDASAFNKENSISSMAPPASPPASPKTPIEKKHADEIDKYIQGLDYNKNNVLVYHGDAVTNVPPRKGYKDGNEYIVVEKKKKSINQNNADIQVVNAISSLTYPGALVKANSELVENQPDVLPVKRDSLTLSIDLPGMTNQDNKIVVKNATKSNVNNAVNTLVERWNEKYAQAYPNVSAKIDYDDEMAYSESQLIAKFGTAFKAVNNSLNVNFGAISEGKMQEEVISFKQIYYNVNVNEPTRPSRFFGKAVTKEQLQALGVNAENPPAYISSVAYGRQVYLKLSTNSHSTKVKAAFDAAVSGKSVSGDVELTNIIKNSSFKAVIYGGSAKDEVQIIDGNLGDLRDILKKGATFNRETPGVPIAYTTNFLKDNELAVIKNNSEYIETTSKAYTDGKINIDHSGGYVAQFNISWDEVNYDPEGNEIVQHKNWSENNKSKLAHFTSSIYLPGNARNINVYAKECTGLAWEWWRTVIDDRNLPLVKNRNISIWGTTLYPKYSNKVDNPIE(GenBankアクセス番号P13128;配列番号:17;核酸配列はGenBankアクセス番号X15127に記載されている)。この配列に対応するプロ蛋白質の最初の25のアミノ酸はシグナル配列であって、それが細菌によって分泌されると、LLOから切断される。かくして、この実施態様において、全長活性LLO蛋白質は504残基長である。もう1つの実施態様において、LLO蛋白質は配列番号:17のホモログである。もう1つの実施態様において、LLO蛋白質は配列番号:17の変種である。もう1つの実施態様において、LLO蛋白質は配列番号:17のイソ形態である。もう1つの実施態様において、LLO蛋白質は配列番号:17の断片である。各可能性は本発明の別の実施態様を表わす。
MKKIMLVFITLILVSLPIAQQTEAKDASAFNKENSISSVAPPASPPASPKTPIEKKHADEIDKYIQGLDYNKNNVLVYHGDAVTNVPPRKGYKDGNEYIVVEKKKKSINQNNADIQVVNAISSLTYPGALVKANSELVENQPDVLPVKRDSLTLSIDLPGMTNQDNKIVVKNATKSNVNNAVNTLVERWNEKYAQAYSNVSAKIDYDDEMAYSESQLIAKFGTAFKAVNNSLNVNFGAISEGKMQEEVISFKQIYYNVNVNEPTRPSRFFGKAVTKEQLQALGVNAENPPAYISSVAYGRQVYLKLSTNSHSTKVKAAFDAAVSGKSVSGDVELTNIIKNSSFKAVIYGGSAKDEVQIIDGNLGDLRDILKKGATFNRETPGVPIAYTTNFLKDNELAVIKNNSEYIETTSKAYTDGKINIDHSGGYVAQFNISWDEVNYD(配列番号:18)を有する。もう1つの実施態様において、LLO断片は配列番号:18のホモログである。もう1つの実施態様において、LLO断片は配列番号:18の変種である。もう1つの実施態様において、LLO断片は配列番号:18のイソ形態である。もう1つの実施態様において、LLO断片は配列番号:18の断片である。各可能性は本発明の別の実施態様を表す。
MKKIMLVFITLILVSLPIAQQTEAKDASAFNKENSISSVAPPASPPASPKTPIEKKHADEIDKYIQGLDYNKNNVLVYHGDAVTNVPPRKGYKDGNEYIVVEKKKKSINQNNADIQVVNAISSLTYPGALVKANSELVENQPDVLPVKRDSLTLSIDLPGMTNQDNKIVVKNATKSNVNNAVNTLVERWNEKYAQAYSNVSAKIDYDDEMAYSESQLIAKFGTAFKAVNNSLNVNFGAISEGKMQEEVISFKQIYYNVNVNEPTRPSRFFGKAVTKEQLQALGVNAENPPAYISSVAYGRQVYLKLSTNSHSTKVKAAFDAAVSGKSVSGDVELTNIIKNSSFKAVIYGGSAKDEVQIIDGNLGDLRDILKKGATFNRETPGVPIAYTTNFLKDNELAVIKNNSEYIETTSKAYTD(配列番号:19)を有する。もう1つの実施態様において、LLO断片は配列番号:19のホモログである。もう1つの実施態様において、LLO断片は配列番号:19の変種である。もう1つの実施態様において、LLO断片は配列番号:19のイソ形態である。もう1つの実施態様において、LLO断片は配列番号:19の断片である。各可能性は本発明の別の実施態様を表わす。
MRAMMVVFITANCITINPDIIFAATDSEDSSLNTDEWEEEKTEEQPSEVNTGPRYETAREVSSRDIKELEKSNKVRNTNKADLIAMLKEKAEKGPNINNNNSEQTENAAINEEASGADRPAIQVERRHPGLPSDSAAEIKKRRKAIASSDSELESLTYPDKPTKVNKKKVAKESVADASESDLDSSMQSADESSPQPLKANQQPFFPKVFKKIKDAGKWVRDKIDENPEVKKAIVDKSAGLIDQLLTKKKSEEVNASDFPPPPTDEELRLALPETPMLLGFNAPATSEPSSFEFPPPPTDEELRLALPETPMLLGFNAPATSEPSSFEFPPPPTEDELEIIRETASSLDSSFTRGDLASLRNAINRHSQNFSDFPPIPTEEELNGRGGRP(配列番号:15)を有する。もう1つの実施態様において、ActA断片は配列番号:15を含む。もう1つの実施態様において、ActA断片は当該分野で公知のいずれかの他のActA断片である。もう1つの実施態様において、ActA蛋白質は配列番号:15のホモログである。もう1つの実施態様において、ActA蛋白質は配列番号:15の変種である。もう1つの実施態様において、ActA蛋白質は配列番号:15のイソ形態である。もう1つの実施態様において、ActA蛋白質は配列番号:15の断片である。もう1つの実施態様において、ActA蛋白質は配列番号:15のホモログの断片である。もう1つの実施態様において、ActA蛋白質は配列番号:15のホモログの断片である。もう1つの実施態様において、ActA蛋白質は配列番号:15の変種の断片である。もう1つの実施態様において、ActA蛋白質は配列番号:15のイソ形態の断片である。各可能性は本発明の別の実施態様を表わす。
MRAMMVVFITANCITINPDIIFAATDSEDSSLNTDEWEEEKTEEQPSEVNTGPRYETAREVSSRDIKELEKSNKVRNTNKADLIAMLKEKAEKGPNINNN(配列番号:14)を有する。もう1つの実施態様において、ActA断片は配列番号:14のホモログである。もう1つの実施態様において、ActA断片は配列番号:14の変種である。もう1つの実施態様において、ActA断片は配列番号:14のイソ形態である。各可能性は本発明の別の実施態様を表わす。
atgcgtgcgatgatggtggttttcattactgccaattgcattacgattaaccccgacataatatttgcagcgacagatagcgaagattctagtctaaacacagatgaatgggaagaagaaaaaacagaagagcaaccaagcgaggtaaatacgggaccaagatacgaaactgcacgtgaagtaagttcacgtgatattaaagaactagaaaaatcgaataaagtgagaaatacgaacaaagcagacctaatagcaatgttgaaagaaaaagcagaaaaaggtccaaatatcaataataacaacagtgaacaaactgagaatgcggctataaatgaagaggcttcaggagccgaccgaccagctatacaagtggagcgtcgtcatccaggattgccatcggatagcgcagcggaaattaaaaaaagaaggaaagccatagcatcatcggatagtgagcttgaaagccttacttatccggataaaccaacaaaagtaaataagaaaaaagtggcgaaagagtcagttgcggatgcttctgaaagtgacttagattctagcatgcagtcagcagatgagtcttcaccacaacctttaaaagcaaaccaacaaccatttttccctaaagtatttaaaaaaataaaagatgcggggaaatgggtacgtgataaaatcgacgaaaatcctgaagtaaagaaagcgattgttgataaaagtgcagggttaattgaccaattattaaccaaaaagaaaagtgaagaggtaaatgcttcggacttcccgccaccacctacggatgaagagttaagacttgctttgccagagacaccaatgcttcttggttttaatgctcctgctacatcagaaccgagctcattcgaatttccaccaccacctacggatgaagagttaagacttgctttgccagagacgccaatgcttcttggttttaatgctcctgctacatcggaaccgagctcgttcgaatttccaccgcctccaacagaagatgaactagaaatcatccgggaaacagcatcctcgctagattctagttttacaagaggggatttagctagtttgagaaatgctattaatcgccatagtcaaaatttctctgatttcccaccaatcccaacagaagaagagttgaacgggagaggcggtagacca(配列番号:16)、これは、1つの実施態様においては、リステリア・モノサイトゲネスにおけるActAをコードする最初の1170ヌクレオチドである。
MRAMMVVFITANCITINPDIIFAATDSEDSSLNTDEWEEEKTEEQPSEVNTGPRYETAREVSSRDIEELEKSNKVKNTNKADLIAMLKAKAEKGPNNNNNNGEQTGNVAINEEASGVDRPTLQVERRHPGLSSDSAAEIKKRRKAIASSDSELESLTYPDKPTKANKRKVAKESVVDASESDLDSSMQSADESTPQPLKANQKPFFPKVFKKIKDAGKWVRDKIDENPEVKKAIVDKSAGLIDQLLTKKKSEEVNASDFPPPPTDEELRLALPETPMLLGFNAPTPSEPSSFEFPPPPTDEELRLALPETPMLLGFNAPATSEPSSFEFPPPPTEDELEIMRETAPSLDSSFTSGDLASLRSAINRHSENFSDFPLIPTEEELNGRGGRP(配列番号:61)を有し、これは、1つの実施態様においては、リステリア・モノサイトゲネス株10403SからのActAについての最初の390AAである、である。もう1つの実施態様において、ActA断片は配列番号:61に記載された配列を含む。もう1つの実施態様において、ActA断片は当該分野で公知のいずれかの他のActA断片である。もう1つの実施態様において、ActA蛋白質は配列番号:61のホモログである。もう1つの実施態様において、ActA蛋白質は配列番号:61の変種である。もう1つの実施態様において、ActA蛋白質は配列番号:61のイソ形態である。もう1つの実施態様において、ActA蛋白質は配列番号:61の断片である。もう1つの実施態様において、ActA蛋白質は配列番号:61のホモログの断片である。もう1つの実施態様において、ActA蛋白質は配列番号:61の変種の断片である。もう1つの実施態様において、ActA蛋白質は配列番号:61のイソ形態の断片である。各可能性は本明細書中で提供される方法および組成物の別の実施態様を表わす。
atgcgtgcgatgatggtagttttcattactgccaactgcattacgattaaccccgacataatatttgcagcgacagatagcgaagattccagtctaaacacagatgaatgggaagaagaaaaaacagaagagcagccaagcgaggtaaatacgggaccaagatacgaaactgcacgtgaagtaagttcacgtgatattgaggaactagaaaaatcgaataaagtgaaaaatacgaacaaagcagacctaatagcaatgttgaaagcaaaagcagagaaaggtccgaataacaataataacaacggtgagcaaacaggaaatgtggctataaatgaagaggcttcaggagtcgaccgaccaactctgcaagtggagcgtcgtcatccaggtctgtcatcggatagcgcagcggaaattaaaaaaagaagaaaagccatagcgtcgtcggatagtgagcttgaaagccttacttatccagataaaccaacaaaagcaaataagagaaaagtggcgaaagagtcagttgtggatgcttctgaaagtgacttagattctagcatgcagtcagcagacgagtctacaccacaacctttaaaagcaaatcaaaaaccatttttccctaaagtatttaaaaaaataaaagatgcggggaaatgggtacgtgataaaatcgacgaaaatcctgaagtaaagaaagcgattgttgataaaagtgcagggttaattgaccaattattaaccaaaaagaaaagtgaagaggtaaatgcttcggacttcccgccaccacctacggatgaagagttaagacttgctttgccagagacaccgatgcttctcggttttaatgctcctactccatcggaaccgagctcattcgaatttccgccgccacctacggatgaagagttaagacttgctttgccagagacgccaatgcttcttggttttaatgctcctgctacatcggaaccgagctcattcgaatttccaccgcctccaacagaagatgaactagaaattatgcgggaaacagcaccttcgctagattctagttttacaagcggggatttagctagtttgagaagtgctattaatcgccatagcgaaaatttctctgatttcccactaatcccaacagaagaagagttgaacgggagaggcggtagacca(配列番号:62)を含み、これは、1つの実施態様においては、リステリア・モノサイトゲネス 10403S株においてActAをコードする最初の1170ヌクレオチドである。もう1つの実施態様において、組換えヌクレオチドは配列番号:62に記載された配列を有する。もう1つの実施態様において、組換えヌクレオチドはActA蛋白質の断片をコードするいずれかの他の配列を含む。各可能性は本明細書中に提供された方法および組成物の別の実施態様を表わす。
[001]MVTGWHRPTWIEIDRAAIRENIKNEQNKLPESVDLWAVVKANAYGHGIIEVARTAKEAGAKGFCVAILDEALALREAGFQDDFILVLGATRKEDANLAAKNHISLTVFREDWLENLTLEATLRIHLKVDSGMGRLGIRTTEEARRIEATSTNDHQLQLEGIYTHFATADQLETSYFEQQLAKFQTILTSLKKRPTYVHTANSAASLLQPQIGFDAIRFGISMYGLTPSTEIKTSLPFELKPALALYTEMVHVKELAPGDSVSYGATYTATEREWVATLPIGYADGLIRHYSGFHVLVDGEPAPIIGRVCMDQTIIKLPREFQTGSKVTIIGKDHGNTVTADDAAQYLDTINYEVTCLLNERIPRKYIH(配列番号:56;GenBankアクセス番号:AF038438)を有する。もう1つの実施態様において、dal蛋白質は配列番号:56に対して相同である。もう1つの実施態様において、dal蛋白質は配列番号:56の変種である。もう1つの実施態様において、dal蛋白質は配列番号:56のイソ形態である。もう1つの実施態様において、dal蛋白質は配列番号:56の断片である。もう1つの実施態様において、dal蛋白質は配列番号:56のホモログの断片である。もう1つの実施態様において、dal蛋白質は配列番号:56の変種の断片である。もう1つの実施態様において、dal蛋白質は配列番号:56のイソ形態の断片である。
MKVLVNNHLVEREDATVDIEDRGYQFGDGVYEVVRLYNGKFFTYNEHIDRLYASAAKIDLVIPYSKEELRELLEKLVAENNINTGNVYLQVTRGVQNPRNHVIPDDFPLEGVLTAAAREVPRNERQFVEGGTAITEEDVRWLRCDIKSLNLLGNILAKNKAHQQNALEAILHRGEQVTECSASNVSIIKDGVLWTHAADNLILNGITRQVIIDVAKKNGIPVKEADFTLTDLREADEVFISSTTIEITPITHIDGVQVADGKRGPITAQLHQYFVEEITRACGELEFAK(配列番号:57;GenBankアクセス番号:AF038439)によってコードされる。もう1つの実施態様において、dat蛋白質は配列番号:57に対して相同である。もう1つの実施態様において、dat蛋白質は配列番号:57の変種である。もう1つの実施態様において、dat蛋白質は配列番号:57のイソ形態である。もう1つの実施態様において、dat蛋白質は配列番号:57の断片である。もう1つの実施態様において、dat蛋白質は配列番号:57のホモログの断片である。もう1つの実施態様において、dat蛋白質は配列番号:57の変種の断片である。もう1つの実施態様において、dat蛋白質は配列番号:57のイソ形態の断片である。
マウス、細胞系および培地
Lm−LLO−PSAのイン・ビボ継代
プラスミド配列決定
TTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCATTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGCTTTCGATTTGTTTTTGAATGGTTTATCCGATAAAGAAGTTGAAGAACAAACTGGAATCAATCGCCGAACGTTTAGAAGGTATCGAGCAAGATATAACGTGACAGTCGATCAAAGAAAAAACAATGAAAAGAGGGATAGTTAATGAGTACGGTTATTTTAGCTGAAAAACCAAGCCAGGCATTAGCCTACGCAAGTGCTTTAAAACAAAGCACCAAAAAAGACGGTTATTTTGAGATCAAAGACCCACTATTTACAGATGAAACGTTTATCACCTTTGGTTTTGGGCATTTAGTGGAATTAGCAGAACCAGGTCATTATGACGAAAAGTGGCAAAATTGGAAACTTGAATCTTTGCCGATTTTTCCTGATCGATACGATTTTGAAGTTGCAAAAGATAAGGGAAAGCAGTTTAAAATTGTTGCAGAACTTCTCAAAAAGGCAAATACAATTATTGTTGCAACAGATAGCGACAGAGAAGGTGAAAATATCGCCTGGTCGATTATCCATAAAGCAAATGCCTTTTCAAAAGATAAAACATTTAAAAGACTATGGATCAATAGCTTAGAAAAAGATGTAATCCGAAGCGGTTTTCAAAATTTGCAACCTGGAATGAATTACTATCCCTTTTATCAAGAAGCGCAAACACGCCAAATTGCCGATTGGTTGATCGGCATGAACGCAAGCCCTTTGTATACGTTAAATTTACAACAGAAGGGCGTACAAGGTACATTTTCACTAGGACGTGTTCAAACGCCCACCTTATACCTTATTTTTCAGCGCCAGGAAGCCATAGAGAATTTTAAAAAAGAACCTTTTTTCGAGGTGGAAGCTAGTATAAAAGTAAACCAAGGGTCGTTTAAGGGCGTTCTAAGCCCCACACAGCGTTTTAAAACCCAAGAGGAGCTTTTAGCTTTTGTTTCTTCTAAACAAGCTAAAATAGGCAATCAAGAGGGGATAATTGCTGATGTTCAAACCAAAGAGAAGAAAACGAATAGTCCGAGTTTGTTTTCTTTAAGTAGTTTGCAATCAAAAGTCAATCAGCTTTATAAAGCGACAGCGAGCCAAACTTTAAAAGCTATTTCTTTTTTAATAACTTAAAAATAAACTTAATGTAACAGCAAGCACAGTCAAGGTATACACCTTTGACAAAAAATAGCACATTCTCTATCGAAAATTTTTGCTTATTTTTTAAATTATTTTGGGAAATTTTCCCAATCCCTTTTTCTAACTCAAAAAATATAATCACTCAAAATTTAAAAGGGCGCACTTATACATCATTTTAAAAAATTGATGTAACGTGCTAAGTTCAAAACAAAGGGCGCACTTATACACGATTTTCAATCTTGTATATTTCTAACGAAAAGCGTGCGCCAAAAAACCCCCTTCGTCAATTTTGACAGGGGGCTTTTTGATGTAAAAATTTCTATCGAAATTTAAAAATTCGCTTCACTCATGTTATAAAGACTTAAAATAAAATAACTCTTTAAAATCTTTTGCTAGTTGTTCTTCAATATTTTTTATTCGGTGCATCTTCCAAGTAAAGTATAACACACTAGACTTATTTACTACGTTTCATAAGTCATTAATGCGTGTGCTCTGCGAGGCTAGTTTTTGTGCAAGCACAAAAAATGGACTGAATAAATCAGTCCATAAGTTCAAAACCAAATTCAAAATCAAAACCACAAGCAACCAAAAAATGTGGTTGTTATACGTTCATAAATTTTATGATCACTTACGTGTATAAAATTAAATTCACTTTCAAAATCTAAAAACTAAATCCAATCATCTACCCTATGAATTATATCTTGAAATTCATTCATAAATAGTGAAGCATGGTAACCATCACATACAGAATGATGAAGTTGCAGAGCAACTGGTATATAAATTTTATTATTCTCACTATAAAATTTACCTATCGTAATAATAGGCAATAAAAAGCTGCTATTGTTACCAATATTTAAATTAAATGAACTAAAATCAATCCAAGGAATCATTGAAATCGGTATGGTGTTTTCAGGTATCGGTTTTTTAGGAAACATTTCTTCTTTATCTTTATATTCAAGCAAGTCATTTTTATAATTATTATAAAAAGAAATGAAGTTTTTATCAGATTCAGTCCAAATGTTAGTAAATTTTTCAGTTTGCTTATTAAAAACTGTATACAAAGGATTTAACTTATCCCAATAACCTAATTTATTCTCACTATTAATTCCTGTTCTAAACACTTTATTTTTATTTACAACTTCCATAATTGCATAAATTAAAGAGGGATAAATTTCATATCCTTTCTTTTTTATCATATCTTTAAACAAAGTAATATCAATTTCTTTAGTAATGCTATAAGTAGTTTGCTGATTAAAATAGTGTTCAAAATATTCTTTTCTATCCCAATTTTCTAATTCAATAATATTAAAAGTCATATATAACTTCCTCCTAAATTTTAAATTTTTATATTTAGGAGGAATAATCCTCTGATTTTTTCATACGTTATGTCACCTCGTAAATATTAATTATACTGAATTAGCAATTTTTATCAAATAAAACTTATTTTACTTCCAAAACCTAAATTCACGTTGCCAAAAATCAATCTGCTTTTGCAATTGTTTTTCGTTCGCTTTTAAAGTCGATTTCATTAATTCCGTTAAATCAATTGGAGATATTTCTCTAATCAATTTTTTAAATTTAGTCTTAGTATTCTTACTTAGCTTTCCCCACATACTTTCTTCATGCAACAAAGTATAAACCATAGCTTGCTCATTAATTTTTTCTAAAGTAGCCCACGCAGGTTTCAAGATGTGTAAATCATTAAAACAATCATTCCAGTAATCAACCATATCTCTTTTTAATTCAACTTCTACACGCCATAAATGTTCAGACACAACTTCAACATCTGCGTTATCTTTACGTTCTTGTTTTTTATTATAAATTCTAATAAATCTATCACTATCACGGACACCAAAATATTTTGTTTCTGGCTTGCCATTACGACCATAAAAAACAGTTTTCTTAACTGCTTTATCAGTCATTGCATAGTAATCGCTCAAATCATCTTCAAAATCAAAAGCTAAGTCTAATCTTGTAAAACCGTCATCTTCCATGTAGTCGATAATATTTTGTTTTAACCAAATCATTTCTTCATGTGTGAGTTTATTGGGATTAAATTCAACACGCATATTACGTCTATCCCAAGTATCTGCTTTTACTTTGTCATATTCGATATAAACTTTTTCTTGAAGTGCCTTAGCTTTAAACTTTGTTTGAAGTATATCCCAAAGTCGTATTTGTGGCTCTACACTCATAAAGTCAGATAGCTTTTTAGCATTAGTTTTGTTCAAATTTCCAACGATTGTCATGGCATCAAAACTTAATGCGGGTTGAGATTTTCCCAAAGTTTGACCACTTAACCGGCTATTACTTAACCGGCTATTAGAGACGGAACTAACTCAACGCTAGTAGTGGATTTAATCCCAAATGAGCCAACAGAACCAGAACCAGAAACAGAACAAGTAACATTGGAGTTAGAAATGGAAGAAGAAAAAAGCAATGATTTCGTGTGAATAATGCACGAAATCATTGCTTATTTTTTTAAAAAGCGATATACTAGATATAACGAAACAACGAACTGAATAAAGAATACAAAAAAAGAGCCACGACCAGTTAAAGCCTGAGAAACTTTAACTGCGAGCCTTAATTGATTACCACCAATCAATTAAAGAAGTCGAGACCCAAAATTTGGTAAAGTATTTAATTACTTTATTAATCAGATACTTAAATATCTGTAAACCCATTATATCGGGTTTTTGAGGGGATTTCAAGTCTTTAAGAAGATACCAGGCAATCAATTAAGAAAAACTTAGTTGATTGCCTTTTTTGTTGTGATTCAACTTTGATCGTAGCTTCTAACTAATTAATTTTCGTAAGAAAGGAGAACAGCTGAATGAATATCCCTTTTGTTGTAGAAACTGTGCTTCATGACGGCTTGTTAAAGTACAAATTTAAAAATAGTAAAATTCGCTCAATCACTACCAAGCCAGGTAAAAGTAAAGGGGCTATTTTTGCGTATCGCTCAAAAAAAAGCATGATTGGCGGACGTGGCGTTGTTCTGACTTCCGAAGAAGCGATTCACGAAAATCAAGATACATTTACGCATTGGACACCAAACGTTTATCGTTATGGTACGTATGCAGACGAAAACCGTTCATACACTAAAGGACATTCTGAAAACAATTTAAGACAAATCAATACCTTCTTTATTGATTTTGATATTCACACGGAAAAAGAAACTATTTCAGCAAGCGATATTTTAACAACAGCTATTGATTTAGGTTTTATGCCTACGTTAATTATCAAATCTGATAAAGGTTATCAAGCATATTTTGTTTTAGAAACGCCAGTCTATGTGACTTCAAAATCAGAATTTAAATCTGTCAAAGCAGCCAAAATAATCTCGCAAAATATCCGAGAATATTTTGGAAAGTCTTTGCCAGTTGATCTAACGTGCAATCATTTTGGGATTGCTCGTATACCAAGAACGGACAATGTAGAATTTTTTGATCCCAATTACCGTTATTCTTTCAAAGAATGGCAAGATTGGTCTTTCAAACAAACAGATAATAAGGGCTTTACTCGTTCAAGTCTAACGGTTTTAAGCGGTACAGAAGGCAAAAAACAAGTAGATGAACCCTGGTTTAATCTCTT
ATTGCACGAAACGAAATTTTCAGGAGAAAAGGGTTTAGTAGGGCGCAATAGCGTTATGTTTACCCTCTCTTTAGCCTACTTTAGTTCAGGCTATTCAATCGAAACGTGCGAATATAATATGTTTGAGTTTAATAATCGATTAGATCAACCCTTAGAAGAAAAAGAAGTAATCAAAATTGTTAGAAGTGCCTATTCAGAAAACTATCAAGGGGCTAATAGGGAATACATTACCATTCTTTGCAAAGCTTGGGTATCAAGTGATTTAACCAGTAAAGATTTATTTGTCCGTCAAGGGTGGTTTAAATTCAAGAAAAAAAGAAGCGAACGTCAACGTGTTCATTTGTCAGAATGGAAAGAAGATTTAATGGCTTATATTAGCGAAAAAAGCGATGTATACAAGCCTTATTTAGCGACGACCAAAAAAGAGATTAGAGAAGTGCTAGGCATTCCTGAACGGACATTAGATAAATTGCTGAAGGTACTGAAGGCGAATCAGGAAATTTTCTTTAAGATTAAACCAGGAAGAAATGGTGGCATTCAACTTGCTAGTGTTAAATCATTGTTGCTATCGATCATTAAATTAAAAAAAGAAGAACGAGAAAGCTATATAAAGGCGCTGACAGCTTCGTTTAATTTAGAACGTACATTTATTCAAGAAACTCTAAACAAATTGGCAGAACGCCCCAAAACGGACCCACAACTCGATTTGTTTAGCTACGATACAGGCTGAAAATAAAACCCGCACTATGCCATTACATTTATATCTATGATACGTGTTTGTTTTTCTTTGCTGTTTAGTGAATGATTAGCAGAAATATACAGAGTAAGATTTTAATTAATTATTAGGGGGAGAAGGAGAGAGTAGCCCGAAAACTTTTAGTTGGCTTGGACTGAACGAAGTGAGGGAAAGGCTACTAAAACGTCGAGGGGCAGTGAGAGCGAAGCGAACACTTGATCTTTTAAGTTGCTATCATTTATAGGTCAATAGAGTATACCTATTTGTCCTAATATGATTTTAGCAGTATAATTGACTTGGTGAATAGGTCATTTAAGTTGGGCATAATAGGAGGAGTAAAATGAAAAAATTTATTTATCGAGTTTTAGAAAATGACGAAGTGGTGGCTATTTTTAATGAGCAACAATATGCGCAAGATTTTATCGCTTACGAAAAGACAATTTCTGATAAGCAATTTGAAATTGAAAAAGTAGATATTGCTGATTGGTTATTGCAACCGAGAGAATTTTAGAGGTTGGTTGAAAATGGCTAAAATTGGTTATGCACGTGTCAGTAGCAAAGAACAGAACTTAGATCGGCAATTACAAGCGTTACAGGGCGTTTCTAAGGTCTTTTCAGACAAATTAAGCGGTCAATCGGTCGAACGCCCACAATTACAAGCTATGCTTAACTATATTCGTGAAGGGGATATTGTTATTGTTACTGAATTAGATCGATTAGGACGAAATAATAAAGAATTAACAGAATTGATGAATCAAATTCAAATTAAGGGGGCAACCCTGGAAGTCTTAAATTTACCCTCAATGAATGGTATTGAAGATGAAAATTTAAGGCGTTTGATTAATAGCCTTGTCATTGAATTGTACAAGTATCAAGCAGAATCAGAACGAAAAAAAATTAAGGAACGTCAGGCACAAGGAATCGAAATTGCTAAGAAAAAAGGCAAATTCAAAGGTCGTCAGCATAAATTTAAAGAAAATGATCCACGTTTAAAGTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCCCCTACGTGCTGCTGAAGTTGCCCGCAACAGAGAGTGGAACCAACCGGTGATACCACGATACTATGACTGAGAGTCAACGCCATGAGCGGCCTCATTTCTTATTCTGAGTTACAACAGTCCGCACCGCTGCCGGTAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCCCAACAGTCCCCCGGCCACGGGGCCTGCCACCATACCCACGCCGAAACAAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTAACCGTTTTTATCAGGCTCTGGGAGGCAGAATAAATGATCATATCGTCAATTATTACCTCCACGGGGAGAGCCTGAGCAAACTGGCCTCAGGCATTTGAGAAGCACACGGTCACACTGCTTCCGGTAGTCAATAAACCGGTAAACCAGCAATAGACATAAGCGGCTATTTAACGACCCTGCCCTGAACCGACGACCGGGTCGAATTTGCTTTCGAATTTCTGCCATTCATCCGCTTATTATCACTTATTCAGGCGTAGCAACCAGGCGTTTAAGGGCACCAATAACTGCCTTAAAAAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGG(配列番号:55)。該配列は、pGG55にクローン化されたPSAの正確に予測された配列にマッチする。LLO−PSAオープンリーディングフレームに下線を施し、より低い場合の文字はPSA単独の配列を示す。
Claims (23)
- カリクレイン関連ペプチダーゼ3(KLK3)融合タンパク質を発現する組換えリステリア株であって、該KLK3融合タンパク質の配列が配列番号:54に記載された配列を含む組換えリステリア株。
- 前記KLK3融合タンパク質は、さらに、非KLK3ペプチドを含み、ここで該非KLK3ペプチドが該断片の免疫原性を増強させる請求項1記載の組換えリステリア株。
- 前記非KLK3ペプチドが非溶血性リステリオリシン(LLO)ペプチド、ActAペプチドまたはPEST配列ペプチドである請求項2記載の組換えリステリア株。
- 前記KLK3融合タンパク質がKLK3シグナル配列を含有しない請求項1乃至3のいずれかに記載の組換えリステリア株。
- 前記組換えリステリア株が動物宿主を通じて継代されている請求項1乃至4のいずれかに記載の組換えリステリア株。
- 前記組換えリステリア株が組換えリステリア・モノサイトゲネス株である請求項5記載の組換えリステリア株。
- 非KLK3ペプチドに操作可能に連結されたカリクレイン関連ペプチダーゼ3(KLK3)ペプチドを含む組換えポリペプチドであって、該非KLK3ペプチドは非溶血性リステリオリシン(LLO)ペプチド、ActAペプチドおよびPEST様アミノ酸配列から選択され、前記組換えポリペプチドの配列が配列番号:54に記載された配列を含む組換えポリペプチド。
- 請求項7記載の組換えポリペプチドを製造する方法であって、
前記組換えポリペプチドをコードするヌクレオチド分子の翻訳の工程を含むことを特徴とする方法。 - 請求項7記載の組換えポリペプチドを製造する方法であって、
前記非KLK3ペプチドを含むポリペプチドに対して、該KLK3ペプチドを含むポリペプチドを化学的に共役させる工程を含むことを特徴とする方法。 - 請求項7記載の組換えポリペプチドをコードするヌクレオチド分子。
- 対象において抗カリクレイン関連ペプチダーゼ3(KLK3)免疫応答を誘導するための組成物の調製における、請求項1乃至6のいずれかに記載の組換えリステリア株、請求項7記載の組換えポリペプチド、または請求項10記載のヌクレオチド分子の使用。
- 対象においてカリクレイン関連ペプチダーゼ3(KLK3)タンパク質発現前立腺癌を治療し、それにより、該対象は該KLK3タンパク質発現前立腺癌に対する免疫応答を高めるための組成物の調製における、請求項1乃至6のいずれかに記載の組換えリステリア株、請求項7記載の組換えポリペプチド、または請求項10記載のヌクレオチド分子の使用。
- カリクレイン関連ペプチダーゼ3(KLK3)タンパク質発現前立腺癌を阻害し、または抑制し、それにより、該対象は該KLK3タンパク質に対する免疫応答を高め、それにより、KLK3タンパク質発現前立腺癌に対してヒト対象を保護するための組成物の調製における、請求項1乃至6のいずれかに記載の組換えリステリア株、請求項7記載の組換えポリペプチド、または請求項10記載のヌクレオチド分子の使用。
- 前記組換えリステリア株が栄養要求性リステリア株である請求項1乃至6のいずれかに記載の組換えリステリア株。
- 前記栄養要求性リステリア株がdal/dat突然変異体である請求項14記載の組換えリステリア株。
- 前記栄養要求性リステリア株が、内因性ActA遺伝子において欠失を含む請求項14または15に記載の組換えリステリア株。
- 前記栄養要求性リステリア株が、該栄養要求性リステリア株の栄養要求性を補充する代謝酵素を含むエピソーム発現ベクターを含む請求項14乃至16のいずれかに記載の組換えリステリア株。
- 前記代謝酵素がアラニンラセマーゼ酵素またはD−アミノ酸トランスフェラーゼ酵素である請求項17記載の組換えリステリア株。
- 請求項1乃至6及び請求項14乃至18のいずれかに記載の組換えリステリア株およびアジュバントを含むワクチン。
- 請求項7記載の組換えポリペプチドおよびアジュバントを含むワクチン。
- 請求項7記載の組換えポリペプチドをコードする組換えワクチンベクター。
- 請求項10記載のヌクレオチド分子、およびアジュバントを含むワクチン。
- 請求項10記載のヌクレオチド分子を含む組換えワクチンベクター。
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US20200069785A1 (en) | 2020-03-05 |
US9549973B2 (en) | 2017-01-24 |
HUE024509T2 (en) | 2016-01-28 |
JP5932751B2 (ja) | 2016-06-08 |
EP2155243A2 (en) | 2010-02-24 |
DK2155243T3 (en) | 2015-03-09 |
WO2008140812A2 (en) | 2008-11-20 |
EP2859899A1 (en) | 2015-04-15 |
US20070253976A1 (en) | 2007-11-01 |
JP2014064569A (ja) | 2014-04-17 |
PL2155243T4 (pl) | 2015-08-31 |
US9012141B2 (en) | 2015-04-21 |
EP2155243B8 (en) | 2015-02-18 |
ES2531967T3 (es) | 2015-03-23 |
WO2008140812A3 (en) | 2009-02-05 |
US11446369B2 (en) | 2022-09-20 |
US20170100469A1 (en) | 2017-04-13 |
EP2155243A4 (en) | 2011-07-27 |
PL2155243T3 (pl) | 2015-08-31 |
EP2155243B1 (en) | 2014-12-03 |
SI2155243T1 (sl) | 2015-06-30 |
US20150335721A1 (en) | 2015-11-26 |
PT2155243E (pt) | 2015-03-30 |
JP2010526533A (ja) | 2010-08-05 |
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