JP2016135784A5 - - Google Patents
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- Publication number
- JP2016135784A5 JP2016135784A5 JP2016012103A JP2016012103A JP2016135784A5 JP 2016135784 A5 JP2016135784 A5 JP 2016135784A5 JP 2016012103 A JP2016012103 A JP 2016012103A JP 2016012103 A JP2016012103 A JP 2016012103A JP 2016135784 A5 JP2016135784 A5 JP 2016135784A5
- Authority
- JP
- Japan
- Prior art keywords
- group
- nucleic acid
- binding
- support
- ligand
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 150000007523 nucleic acids Chemical class 0.000 claims 9
- 102000039446 nucleic acids Human genes 0.000 claims 9
- 108020004707 nucleic acids Proteins 0.000 claims 9
- 238000000034 method Methods 0.000 claims 6
- 239000003446 ligand Substances 0.000 claims 4
- 238000012986 modification Methods 0.000 claims 4
- 230000004048 modification Effects 0.000 claims 4
- BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical compound [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 claims 3
- 150000001875 compounds Chemical class 0.000 claims 3
- 229910000077 silane Inorganic materials 0.000 claims 3
- UNVFWCQQWZUPLB-UHFFFAOYSA-N 3-[dimethoxy(pentan-3-yloxy)silyl]propan-1-amine Chemical compound CCC(CC)O[Si](OC)(OC)CCCN UNVFWCQQWZUPLB-UHFFFAOYSA-N 0.000 claims 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims 2
- 238000010828 elution Methods 0.000 claims 2
- 239000000463 material Substances 0.000 claims 2
- JKNCOURZONDCGV-UHFFFAOYSA-N 2-(dimethylamino)ethyl 2-methylprop-2-enoate Chemical compound CN(C)CCOC(=O)C(C)=C JKNCOURZONDCGV-UHFFFAOYSA-N 0.000 claims 1
- WIYVVIUBKNTNKG-UHFFFAOYSA-N 6,7-dimethoxy-3,4-dihydronaphthalene-2-carboxylic acid Chemical compound C1CC(C(O)=O)=CC2=C1C=C(OC)C(OC)=C2 WIYVVIUBKNTNKG-UHFFFAOYSA-N 0.000 claims 1
- 150000001252 acrylic acid derivatives Chemical class 0.000 claims 1
- 125000003277 amino group Chemical group 0.000 claims 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 150000004756 silanes Chemical class 0.000 claims 1
- 239000000377 silicon dioxide Substances 0.000 claims 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE102008063001A DE102008063001A1 (de) | 2008-12-23 | 2008-12-23 | Nukleinsäureaufreinigungsverfahren |
| DE102008063001.2 | 2008-12-23 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011541520A Division JP2012513386A (ja) | 2008-12-23 | 2009-12-23 | 核酸の精製方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016135784A JP2016135784A (ja) | 2016-07-28 |
| JP2016135784A5 true JP2016135784A5 (enExample) | 2016-09-29 |
| JP6072945B2 JP6072945B2 (ja) | 2017-02-01 |
Family
ID=42078045
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011541520A Withdrawn JP2012513386A (ja) | 2008-12-23 | 2009-12-23 | 核酸の精製方法 |
| JP2016012103A Active JP6072945B2 (ja) | 2008-12-23 | 2016-01-26 | 核酸の精製方法 |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011541520A Withdrawn JP2012513386A (ja) | 2008-12-23 | 2009-12-23 | 核酸の精製方法 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US9102935B2 (enExample) |
| EP (1) | EP2382315B1 (enExample) |
| JP (2) | JP2012513386A (enExample) |
| CN (1) | CN102264902B (enExample) |
| AU (1) | AU2009332900B2 (enExample) |
| DE (1) | DE102008063001A1 (enExample) |
| WO (1) | WO2010072834A1 (enExample) |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102008063003A1 (de) * | 2008-12-23 | 2010-06-24 | Qiagen Gmbh | Nukleinsäureaufreinigungsverfahren |
| JP5899731B2 (ja) | 2011-09-13 | 2016-04-06 | ソニー株式会社 | 核酸精製方法、核酸抽出方法、及び核酸精製用キット |
| WO2013045434A1 (en) | 2011-09-26 | 2013-04-04 | Qiagen Gmbh | Methods for separating nucleic acids by size |
| CN113388605A (zh) | 2011-09-26 | 2021-09-14 | 凯杰有限公司 | 用于分离细胞外核酸的快速方法 |
| EP2771462A4 (en) * | 2011-10-27 | 2015-04-08 | Ge Healthcare Bio Sciences Ab | PURIFICATION OF NUCLEIC ACIDS |
| KR20150023331A (ko) * | 2012-05-31 | 2015-03-05 | 에이전시 포 사이언스, 테크놀로지 앤드 리서치 | 음으로 대전된 입자들로의 폴리뉴클레오티드들의 크로마토그래피 정제 |
| CN103789297A (zh) * | 2012-10-26 | 2014-05-14 | 上海医脉赛科技有限公司 | 快速纯化核酸的方法及试剂盒 |
| WO2014090838A1 (en) | 2012-12-11 | 2014-06-19 | Qiagen Gmbh | Preparation of silica particles |
| US10590161B2 (en) | 2013-03-15 | 2020-03-17 | Modernatx, Inc. | Ion exchange purification of mRNA |
| US10138507B2 (en) | 2013-03-15 | 2018-11-27 | Modernatx, Inc. | Manufacturing methods for production of RNA transcripts |
| US10077439B2 (en) | 2013-03-15 | 2018-09-18 | Modernatx, Inc. | Removal of DNA fragments in mRNA production process |
| WO2016040697A1 (en) * | 2014-09-10 | 2016-03-17 | Quantumdx Group Limited | Sorbent material for separating bio-macromolecules |
| JP2017539080A (ja) | 2014-10-23 | 2017-12-28 | コーニング インコーポレイテッド | 高分子被包磁性ナノ粒子 |
| WO2016152763A1 (ja) * | 2015-03-20 | 2016-09-29 | 東レ株式会社 | 核酸の回収方法 |
| ES2872549T3 (es) | 2015-06-10 | 2021-11-02 | Qiagen Gmbh | Método para aislar ácidos nucleicos extracelulares usando partículas de intercambio aniónico |
| US20200291385A1 (en) | 2016-03-19 | 2020-09-17 | Qiagen Gmbh | Stabilization of rna |
| US12385034B2 (en) | 2016-06-24 | 2025-08-12 | Modernatx, Inc. | Methods and apparatus for filtration |
| US11685915B2 (en) | 2017-12-27 | 2023-06-27 | Toray Industries, Inc. | Method of collecting nucleic acid |
| CN108102881A (zh) * | 2018-02-05 | 2018-06-01 | 中国科学院苏州纳米技术与纳米仿生研究所 | 具有微结构阵列的微流控芯片及核酸固相纯化提取方法 |
| CN108795929A (zh) * | 2018-07-06 | 2018-11-13 | 大连元和健路医学检验实验室有限公司 | 一种dna分子筛柱子的制备方法 |
| CN108456678A (zh) * | 2018-07-06 | 2018-08-28 | 大连元和健路医学检验实验室有限公司 | 一种快速提取基因组dna的方法 |
| CN113677432B (zh) * | 2019-03-29 | 2024-06-07 | 住友化学株式会社 | 无机多孔质担载体、及使用其的核酸的制造方法 |
| WO2021122846A1 (en) | 2019-12-16 | 2021-06-24 | Qiagen Gmbh | Enrichment method |
| US20240287498A1 (en) | 2021-06-17 | 2024-08-29 | Qiagen Gmbh | Method for isolating non-vesicular mirna |
| CN118339287A (zh) | 2021-11-12 | 2024-07-12 | 舒万诺知识产权公司 | 加工多核酸的方法 |
| CN115537416A (zh) * | 2022-09-29 | 2022-12-30 | 杭州联川生物技术股份有限公司 | 一种纯化寡核苷酸的方法和试剂盒 |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4767670A (en) * | 1987-01-21 | 1988-08-30 | E. I. Du Pont De Nemours And Company | Chromatographic supports for separation of oligonucleotides |
| US5079600A (en) * | 1987-03-06 | 1992-01-07 | Schnur Joel M | High resolution patterning on solid substrates |
| EP0755400A1 (en) * | 1994-04-08 | 1997-01-29 | HYBRIDON, Inc. | Purification of oligodeoxynucleotide phosphorothioates using anion exchange chromatography |
| US5582988A (en) | 1994-09-15 | 1996-12-10 | Johnson & Johnson Clinical Diagnostics, Inc. | Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same |
| US5652348A (en) * | 1994-09-23 | 1997-07-29 | Massey University | Chromatographic resins and methods for using same |
| JP3689896B2 (ja) * | 1995-12-04 | 2005-08-31 | Ｊｓｒ株式会社 | 核酸吸着剤 |
| US6103479A (en) * | 1996-05-30 | 2000-08-15 | Cellomics, Inc. | Miniaturized cell array methods and apparatus for cell-based screening |
| US6914137B2 (en) * | 1997-12-06 | 2005-07-05 | Dna Research Innovations Limited | Isolation of nucleic acids |
| ATE218140T1 (de) | 1997-12-06 | 2002-06-15 | Dna Res Innovations Ltd | Isolierung von nukleinsäuren |
| US6310199B1 (en) * | 1999-05-14 | 2001-10-30 | Promega Corporation | pH dependent ion exchange matrix and method of use in the isolation of nucleic acids |
| JP2003104996A (ja) * | 2001-09-28 | 2003-04-09 | Hitachi Maxell Ltd | 核酸結合用磁性担体およびその製造方法 |
| KR100714988B1 (ko) | 2005-08-09 | 2007-05-09 | 삼성전자주식회사 | 산화막이 표면에 형성된 실리콘 구조물 표면을 이용한ｄｎａ 정제 방법 및 정제장치 |
| WO2008097342A2 (en) * | 2006-07-31 | 2008-08-14 | Sigma-Aldrich Co. | Compositions and methods for isolation of biological molecules |
| WO2008035991A2 (en) * | 2006-09-19 | 2008-03-27 | Michael Ronald Cook | A nucleic acid extraction method |
-
2008
- 2008-12-23 DE DE102008063001A patent/DE102008063001A1/de not_active Withdrawn
-
2009
- 2009-12-23 EP EP09804132.0A patent/EP2382315B1/de active Active
- 2009-12-23 AU AU2009332900A patent/AU2009332900B2/en active Active
- 2009-12-23 WO PCT/EP2009/067909 patent/WO2010072834A1/de not_active Ceased
- 2009-12-23 CN CN200980152958.XA patent/CN102264902B/zh active Active
- 2009-12-23 JP JP2011541520A patent/JP2012513386A/ja not_active Withdrawn
- 2009-12-23 US US13/141,878 patent/US9102935B2/en active Active
-
2016
- 2016-01-26 JP JP2016012103A patent/JP6072945B2/ja active Active
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