JP2015518735A5 - - Google Patents

Download PDF

Info

Publication number
JP2015518735A5
JP2015518735A5 JP2015516255A JP2015516255A JP2015518735A5 JP 2015518735 A5 JP2015518735 A5 JP 2015518735A5 JP 2015516255 A JP2015516255 A JP 2015516255A JP 2015516255 A JP2015516255 A JP 2015516255A JP 2015518735 A5 JP2015518735 A5 JP 2015518735A5
Authority
JP
Japan
Prior art keywords
temperature
mixture
polynucleotide
amplification
egta
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2015516255A
Other languages
English (en)
Japanese (ja)
Other versions
JP2015518735A (ja
JP6861465B2 (ja
Filing date
Publication date
Application filed filed Critical
Priority claimed from PCT/US2013/044796 external-priority patent/WO2013185081A1/en
Publication of JP2015518735A publication Critical patent/JP2015518735A/ja
Publication of JP2015518735A5 publication Critical patent/JP2015518735A5/ja
Application granted granted Critical
Publication of JP6861465B2 publication Critical patent/JP6861465B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

JP2015516255A 2012-06-08 2013-06-07 核酸増幅 Active JP6861465B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261657227P 2012-06-08 2012-06-08
US61/657,227 2012-06-08
US201361782199P 2013-03-14 2013-03-14
US61/782,199 2013-03-14
PCT/US2013/044796 WO2013185081A1 (en) 2012-06-08 2013-06-07 Nucleic acid amplifications

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2019017903A Division JP2019115344A (ja) 2012-06-08 2019-02-04 核酸増幅

Publications (3)

Publication Number Publication Date
JP2015518735A JP2015518735A (ja) 2015-07-06
JP2015518735A5 true JP2015518735A5 (enExample) 2016-07-21
JP6861465B2 JP6861465B2 (ja) 2021-04-21

Family

ID=49712703

Family Applications (4)

Application Number Title Priority Date Filing Date
JP2015516255A Active JP6861465B2 (ja) 2012-06-08 2013-06-07 核酸増幅
JP2019017903A Pending JP2019115344A (ja) 2012-06-08 2019-02-04 核酸増幅
JP2021012803A Pending JP2021072833A (ja) 2012-06-08 2021-01-29 核酸増幅
JP2024082357A Pending JP2024109739A (ja) 2012-06-08 2024-05-21 核酸増幅

Family Applications After (3)

Application Number Title Priority Date Filing Date
JP2019017903A Pending JP2019115344A (ja) 2012-06-08 2019-02-04 核酸増幅
JP2021012803A Pending JP2021072833A (ja) 2012-06-08 2021-01-29 核酸増幅
JP2024082357A Pending JP2024109739A (ja) 2012-06-08 2024-05-21 核酸増幅

Country Status (8)

Country Link
US (3) US10927393B2 (enExample)
EP (2) EP3778915A1 (enExample)
JP (4) JP6861465B2 (enExample)
CN (2) CN104662159B (enExample)
AU (4) AU2013271404B2 (enExample)
CA (2) CA2876159C (enExample)
ES (1) ES2823551T3 (enExample)
WO (1) WO2013185081A1 (enExample)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE60322044D1 (de) 2002-02-21 2008-08-21 Asm Scient Inc Rekombinase-polymerase-amplifikation
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
JP5027126B2 (ja) 2005-07-25 2012-09-19 アリーア サン ディエゴ， インコーポレイテッド リコンビナーゼポリメラーゼ増幅を多重化するための方法
JP2009535053A (ja) 2006-05-04 2009-10-01 エーエスエム サイエンティフィック， インコーポレイテッド レコンビナーゼポリメラーゼ増幅
JP5847076B2 (ja) 2009-05-20 2016-01-20 バイオサイト インコーポレイテッド Ｄｎａグリコシラーゼ／リアーゼおよびａｐエンドヌクレアーゼ基質
EP3360974B1 (en) 2009-06-05 2025-04-09 Abbott Diagnostics Scarborough, Inc. Recombinase polymerase amplification reagents
EP3778915A1 (en) 2012-06-08 2021-02-17 Ionian Technologies, LLC Nucleic acid amplifications
WO2015116686A1 (en) 2014-01-29 2015-08-06 Agilent Technologies, Inc. Cas9-based isothermal method of detection of specific dna sequence
JP2017504336A (ja) * 2014-01-31 2017-02-09 キアゲン ゲーエムベーハー カチオンキレーターホットスタート
WO2016004333A2 (en) * 2014-07-02 2016-01-07 Promega Corporation Reversible metal ion chelators
EP3233128A4 (en) * 2014-12-18 2018-05-23 GE Healthcare UK Limited Analyte detection on a solid support by nucleic acid amplification coupled to an immunoassay
GB201515355D0 (en) * 2015-08-28 2015-10-14 Ge Healthcare Ltd A method and kit for analyte detection
US10329601B2 (en) * 2015-12-28 2019-06-25 Ionian Technologies, Inc. Nicking and extension amplification reaction (NEAR) of Streptococcus species
US11118206B2 (en) 2016-01-13 2021-09-14 The Trustees Of The University Of Pennsylvania Multiple stage isothermal enzymatic amplification
WO2017141067A1 (en) 2016-02-16 2017-08-24 The University Of Tokyo Method of eliminating background amplification of nucleic acid targets
US11299777B2 (en) 2016-04-04 2022-04-12 Nat Diagnostics, Inc. Isothermal amplification components and processes
US9617587B1 (en) 2016-04-04 2017-04-11 Nat Diagnostics, Inc. Isothermal amplification components and processes
CN106093415B (zh) * 2016-04-29 2017-12-19 浙江大学 一种蛋白实时定量检测方法
CN106053403B (zh) * 2016-04-29 2019-05-03 浙江大学 一种蛋白定量检测方法
GB201611469D0 (en) 2016-06-30 2016-08-17 Lumiradx Tech Ltd Improvements in or relating to nucleic acid amplification processes
AU2018227436A1 (en) * 2017-02-28 2019-09-12 Abbott Diagnostics Scarborough, Inc. Microfluidic devices and related methods
GB2569965A (en) 2018-01-04 2019-07-10 Lumiradx Uk Ltd Improvements in or relating to amplification of nucleic acids
CN111979303A (zh) * 2020-08-11 2020-11-24 上海奕谱生物科技有限公司 一种核酸检测试剂盒、方法及其应用
WO2025171104A1 (en) * 2024-02-08 2025-08-14 Alere San Diego, Inc. Universal hairpin probes

Family Cites Families (53)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5693517A (en) * 1987-06-17 1997-12-02 Roche Molecular Systems, Inc. Reagents and methods for coupled high temperature reverse transcription and polymerase chain reactions
ES2100945T3 (es) * 1989-12-22 1997-07-01 Hoffmann La Roche Transcriptasas reversas de alta temperatura.
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US5556751A (en) 1991-04-25 1996-09-17 Amoco Corporation Selective amplification system using Q-β replicase
WO1994003624A1 (en) 1992-08-04 1994-02-17 Auerbach Jeffrey I Methods for the isothermal amplification of nucleic acid molecules
US5614389A (en) 1992-08-04 1997-03-25 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
US5834202A (en) 1992-08-04 1998-11-10 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
US5733733A (en) 1992-08-04 1998-03-31 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
US5942391A (en) 1994-06-22 1999-08-24 Mount Sinai School Of Medicine Nucleic acid amplification method: ramification-extension amplification method (RAM)
US5916779A (en) 1995-09-21 1999-06-29 Becton, Dickinson And Company Strand displacement amplification of RNA targets
US5731150A (en) 1995-11-01 1998-03-24 Chiron Diagnostic Corporation IS6110 based molecular detection of mycobacterium tuberculosis
US6197557B1 (en) * 1997-03-05 2001-03-06 The Regents Of The University Of Michigan Compositions and methods for analysis of nucleic acids
WO1998045474A1 (en) 1997-04-04 1998-10-15 Innogenetics N.V. Isothermal polymerase chain reaction by cycling the concentration of divalent metal ions
US6245506B1 (en) 1997-07-30 2001-06-12 Bbi Bioseq, Inc. Integrated sequencing device
CN1099464C (zh) * 2000-11-28 2003-01-22 复旦大学 一种端粒酶活性检测方法
US6448085B1 (en) * 2001-04-13 2002-09-10 Sysmex Corporation Quality control material and calibrator for nucleated red blood cell tested on hematology analyzer
US20020172972A1 (en) * 2001-05-15 2002-11-21 President And Fellows Of Harvard College Use of a selectively inactivatable enzyme to digest contaminating nucleic acid
US7112423B2 (en) 2001-07-15 2006-09-26 Keck Graduate Institute Nucleic acid amplification using nicking agents
JP2005516610A (ja) 2001-07-15 2005-06-09 ケック グラデュエイト インスティテュート ニック形成剤を用いる遺伝子発現分析
US6403341B1 (en) * 2001-08-02 2002-06-11 Wayne M. Barnes Magnesium precipitate hot start method for PCR
WO2003012066A2 (en) * 2001-08-02 2003-02-13 Barnes Wayne M Magnesium precipitate hot start method for molecular manipulation of nucleic acids
US8030000B2 (en) * 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
DE60322044D1 (de) 2002-02-21 2008-08-21 Asm Scient Inc Rekombinase-polymerase-amplifikation
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
US20040038213A1 (en) 2002-08-06 2004-02-26 Kwon Jai W. Genotyping by in situ PCR amplification of a polynucleotide in a tissue biopsy
EP1539979B1 (en) 2002-09-20 2008-11-19 New England Biolabs, Inc. Helicase dependent amplification of nucleic acids
US7662594B2 (en) 2002-09-20 2010-02-16 New England Biolabs, Inc. Helicase-dependent amplification of RNA
EP1658374A4 (en) * 2003-07-29 2007-04-11 Sigma Aldrich Co METHOD AND COMPOSITIONS FOR AMPLIFICATION OF DNA
US7700281B2 (en) * 2004-06-30 2010-04-20 Usb Corporation Hot start nucleic acid amplification
GB2416352B (en) 2004-07-21 2009-01-28 Bioline Ltd A method for performing the hot start of enzymatic reactions
JP5027126B2 (ja) 2005-07-25 2012-09-19 アリーア サン ディエゴ， インコーポレイテッド リコンビナーゼポリメラーゼ増幅を多重化するための方法
JP5106416B2 (ja) * 2006-01-06 2012-12-26 アジレント・テクノロジーズ・インク 濃縮（ｐａｃｋｅｄ）ＤＮＡポリメラーゼを含む核酸複製のための反応緩衝液組成物
EP1989324B1 (en) * 2006-02-27 2011-05-18 Roche Diagnostics GmbH Pcr hot start by magnesium sequestration
JP2009535053A (ja) 2006-05-04 2009-10-01 エーエスエム サイエンティフィック， インコーポレイテッド レコンビナーゼポリメラーゼ増幅
US9481912B2 (en) * 2006-09-12 2016-11-01 Longhorn Vaccines And Diagnostics, Llc Compositions and methods for detecting and identifying nucleic acid sequences in biological samples
US8357488B2 (en) 2007-05-18 2013-01-22 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services, Centers For Disease Control And Prevention Primers and probes for the detection of streptococcus pneumoniae
US9689031B2 (en) 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
KR101414713B1 (ko) * 2007-10-11 2014-07-03 삼성전자주식회사 리가제 및 엔도뉴클레아제의 존재하에서 롤링서클 증폭에의하여 표적 핵산을 증폭하는 방법
JP2009139487A (ja) 2007-12-04 2009-06-25 Nippon Sheet Glass Co Ltd 正立等倍レンズアレイプレート
US8911948B2 (en) * 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
JP5572943B2 (ja) * 2008-12-17 2014-08-20 株式会社島津製作所 血液検体からのｄｎａ増幅方法及びｄｎａ増幅キット
EP2418289B1 (en) * 2009-03-31 2015-01-14 Toppan Printing Co., Ltd. Method of distinguishing genotypes
EP3360974B1 (en) 2009-06-05 2025-04-09 Abbott Diagnostics Scarborough, Inc. Recombinase polymerase amplification reagents
WO2011019964A1 (en) * 2009-08-12 2011-02-17 Nugen Technologies, Inc. Methods, compositions, and kits for generating nucleic acid products substantially free of template nucleic acid
JP2013516192A (ja) * 2010-01-08 2013-05-13 キアジェン ゲイサーズバーグ インコーポレイテッド 等温核酸増幅のための材料および方法
CA2837554A1 (en) * 2011-06-03 2012-12-06 Genomic Vision Assessment of cancer risk based on rnu2 cnv and interplay between rnu2 cnv and brca1
SG11201401628WA (en) * 2011-10-19 2014-05-29 Nugen Technologies Inc Compositions and methods for directional nucleic acid amplification and sequencing
US20130217071A1 (en) * 2011-12-30 2013-08-22 Luz Montesclaros Methods and compositions for performing nucleic acid amplification reactions
KR101870311B1 (ko) 2012-03-09 2018-06-25 (주)바이오니아 핫스타트 역전사반응 또는 핫스타트 역전사 중합효소 연쇄반응용 조성물
EP3778915A1 (en) 2012-06-08 2021-02-17 Ionian Technologies, LLC Nucleic acid amplifications
JP2017504336A (ja) * 2014-01-31 2017-02-09 キアゲン ゲーエムベーハー カチオンキレーターホットスタート
WO2016004333A2 (en) * 2014-07-02 2016-01-07 Promega Corporation Reversible metal ion chelators

Similar Documents

Publication Publication Date Title
JP2015518735A5 (enExample)
Shaw et al. The use of carrier RNA to enhance DNA extraction from microfluidic-based silica monoliths
JP2018117645A5 (enExample)
AU2018234832A1 (en) CRISPR effector system based diagnostics for virus detection
HRP20160923T1 (hr) Oslobađanje amlikona višestrukom (multiplex) lančanom reakcijom polimeraze za umnožavanje više ciljeva
JP2014520544A5 (enExample)
RU2015123459A (ru) Одновременная детекция белка-мишени и нуклеиновых кислот-мишеней в одной клетке
NZ629701A (en) Target detection and signal amplification
WO2015081229A3 (en) Selective amplification of nucleic acid sequences
JP2015156872A5 (enExample)
JP2014513534A5 (enExample)
Oreskovic et al. Ultrasensitive hybridization capture: reliable detection of< 1 copy/mL short cell-free DNA from large-volume urine samples
JP2017536846A5 (enExample)
DE602006004435D1 (de) Polymorphismusnachweisverfahren
CA2934641A1 (en) Nucleic acid detection method and kit
DK1649051T3 (da) Bestemmelse af sekvensen for et polynukleotid
JP2017521086A5 (enExample)
WO2012056227A3 (en) Multiplex amplification and detection
JP2014511692A5 (enExample)
WO2014143714A3 (en) Methods for one step nucleic acid amplification of non-eluted samples
CN103571823A (zh) 用离子液体增强生物材料的核酸扩增效率和灵敏度的方法
JP2019193655A (ja) カチオンキレーターホットスタート
MX2017015094A (es) Biosensor electroquimico.
CN107075585A8 (zh) 具有锁核酸的序列转换和信号扩增dna以及使用其的检测方法
WO2009034842A1 (ja) 核酸検出方法、および核酸検出キット