JP2015518735A5 - - Google Patents
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- Publication number
- JP2015518735A5 JP2015518735A5 JP2015516255A JP2015516255A JP2015518735A5 JP 2015518735 A5 JP2015518735 A5 JP 2015518735A5 JP 2015516255 A JP2015516255 A JP 2015516255A JP 2015516255 A JP2015516255 A JP 2015516255A JP 2015518735 A5 JP2015518735 A5 JP 2015518735A5
- Authority
- JP
- Japan
- Prior art keywords
- temperature
- mixture
- polynucleotide
- amplification
- egta
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 38
- 239000000203 mixture Substances 0.000 claims 15
- 239000003153 chemical reaction reagent Substances 0.000 claims 14
- 230000003321 amplification Effects 0.000 claims 12
- 238000003199 nucleic acid amplification method Methods 0.000 claims 12
- 239000002157 polynucleotide Substances 0.000 claims 12
- 102000040430 polynucleotide Human genes 0.000 claims 12
- 108091033319 polynucleotide Proteins 0.000 claims 12
- DEFVIWRASFVYLL-UHFFFAOYSA-N ethylene glycol bis(2-aminoethyl)tetraacetic acid Chemical compound OC(=O)CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O DEFVIWRASFVYLL-UHFFFAOYSA-N 0.000 claims 10
- 150000002500 ions Chemical class 0.000 claims 10
- 239000011230 binding agent Substances 0.000 claims 8
- 239000002738 chelating agent Substances 0.000 claims 7
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims 5
- 239000000872 buffer Substances 0.000 claims 4
- 239000011541 reaction mixture Substances 0.000 claims 4
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims 2
- 230000000694 effects Effects 0.000 claims 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 1
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 claims 1
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 claims 1
- 102100031780 Endonuclease Human genes 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims 1
- 101710147059 Nicking endonuclease Proteins 0.000 claims 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims 1
- 229910052793 cadmium Inorganic materials 0.000 claims 1
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 claims 1
- 229910052791 calcium Inorganic materials 0.000 claims 1
- 239000011575 calcium Substances 0.000 claims 1
- 229910052802 copper Inorganic materials 0.000 claims 1
- 239000010949 copper Substances 0.000 claims 1
- 238000001514 detection method Methods 0.000 claims 1
- 229910052742 iron Inorganic materials 0.000 claims 1
- 229910052749 magnesium Inorganic materials 0.000 claims 1
- 239000011777 magnesium Substances 0.000 claims 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 claims 1
- 239000002773 nucleotide Substances 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
- 239000000243 solution Substances 0.000 claims 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims 1
- 239000011701 zinc Substances 0.000 claims 1
- 229910052725 zinc Inorganic materials 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261657227P | 2012-06-08 | 2012-06-08 | |
| US61/657,227 | 2012-06-08 | ||
| US201361782199P | 2013-03-14 | 2013-03-14 | |
| US61/782,199 | 2013-03-14 | ||
| PCT/US2013/044796 WO2013185081A1 (en) | 2012-06-08 | 2013-06-07 | Nucleic acid amplifications |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019017903A Division JP2019115344A (ja) | 2012-06-08 | 2019-02-04 | 核酸増幅 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015518735A JP2015518735A (ja) | 2015-07-06 |
| JP2015518735A5 true JP2015518735A5 (enExample) | 2016-07-21 |
| JP6861465B2 JP6861465B2 (ja) | 2021-04-21 |
Family
ID=49712703
Family Applications (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015516255A Active JP6861465B2 (ja) | 2012-06-08 | 2013-06-07 | 核酸増幅 |
| JP2019017903A Pending JP2019115344A (ja) | 2012-06-08 | 2019-02-04 | 核酸増幅 |
| JP2021012803A Pending JP2021072833A (ja) | 2012-06-08 | 2021-01-29 | 核酸増幅 |
| JP2024082357A Pending JP2024109739A (ja) | 2012-06-08 | 2024-05-21 | 核酸増幅 |
Family Applications After (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019017903A Pending JP2019115344A (ja) | 2012-06-08 | 2019-02-04 | 核酸増幅 |
| JP2021012803A Pending JP2021072833A (ja) | 2012-06-08 | 2021-01-29 | 核酸増幅 |
| JP2024082357A Pending JP2024109739A (ja) | 2012-06-08 | 2024-05-21 | 核酸増幅 |
Country Status (8)
| Country | Link |
|---|---|
| US (3) | US10927393B2 (enExample) |
| EP (2) | EP3778915A1 (enExample) |
| JP (4) | JP6861465B2 (enExample) |
| CN (2) | CN114214394A (enExample) |
| AU (4) | AU2013271404B2 (enExample) |
| CA (2) | CA3096342C (enExample) |
| ES (1) | ES2823551T3 (enExample) |
| WO (1) | WO2013185081A1 (enExample) |
Families Citing this family (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7399590B2 (en) | 2002-02-21 | 2008-07-15 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| AU2003215391B2 (en) | 2002-02-21 | 2007-05-24 | Abbott Diagnostics Scarborough, Inc. | Recombinase Polymerase Amplification |
| US8030000B2 (en) | 2002-02-21 | 2011-10-04 | Alere San Diego, Inc. | Recombinase polymerase amplification |
| CA2616241C (en) | 2005-07-25 | 2012-02-07 | Asm Scientific, Inc. | Methods for multiplexing recombinase polymerase amplification |
| CA2650993C (en) | 2006-05-04 | 2015-06-16 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| WO2010135310A1 (en) | 2009-05-20 | 2010-11-25 | Biosite Incorporated | Dna glycosylase/lyase and ap endonuclease substrates |
| EP2438196B1 (en) | 2009-06-05 | 2016-12-21 | Alere San Diego, Inc. | Recombinase polymerase amplification reagents and kits |
| CN114214394A (zh) | 2012-06-08 | 2022-03-22 | 爱奥尼安技术公司 | 核苷酸扩增反应 |
| US9850525B2 (en) | 2014-01-29 | 2017-12-26 | Agilent Technologies, Inc. | CAS9-based isothermal method of detection of specific DNA sequence |
| EP3099810B1 (en) * | 2014-01-31 | 2019-05-01 | Qiagen GmbH | Cation chelator hot start |
| WO2016004333A2 (en) * | 2014-07-02 | 2016-01-07 | Promega Corporation | Reversible metal ion chelators |
| US11008604B2 (en) * | 2014-12-18 | 2021-05-18 | Global Life Sciences Solutions Operations UK Ltd | Analyte detection on a solid support by nucleic acid amplification coupled to an immunoassay |
| GB201515355D0 (en) * | 2015-08-28 | 2015-10-14 | Ge Healthcare Ltd | A method and kit for analyte detection |
| US10329601B2 (en) * | 2015-12-28 | 2019-06-25 | Ionian Technologies, Inc. | Nicking and extension amplification reaction (NEAR) of Streptococcus species |
| US11118206B2 (en) | 2016-01-13 | 2021-09-14 | The Trustees Of The University Of Pennsylvania | Multiple stage isothermal enzymatic amplification |
| WO2017141067A1 (en) | 2016-02-16 | 2017-08-24 | The University Of Tokyo | Method of eliminating background amplification of nucleic acid targets |
| US9617587B1 (en) | 2016-04-04 | 2017-04-11 | Nat Diagnostics, Inc. | Isothermal amplification components and processes |
| US11299777B2 (en) | 2016-04-04 | 2022-04-12 | Nat Diagnostics, Inc. | Isothermal amplification components and processes |
| CN106093415B (zh) * | 2016-04-29 | 2017-12-19 | 浙江大学 | 一种蛋白实时定量检测方法 |
| CN106053403B (zh) * | 2016-04-29 | 2019-05-03 | 浙江大学 | 一种蛋白定量检测方法 |
| GB201611469D0 (en) | 2016-06-30 | 2016-08-17 | Lumiradx Tech Ltd | Improvements in or relating to nucleic acid amplification processes |
| AU2018227436A1 (en) * | 2017-02-28 | 2019-09-12 | Abbott Diagnostics Scarborough, Inc. | Microfluidic devices and related methods |
| GB2569965A (en) | 2018-01-04 | 2019-07-10 | Lumiradx Uk Ltd | Improvements in or relating to amplification of nucleic acids |
| CN111979303A (zh) * | 2020-08-11 | 2020-11-24 | 上海奕谱生物科技有限公司 | 一种核酸检测试剂盒、方法及其应用 |
| WO2025171104A1 (en) * | 2024-02-08 | 2025-08-14 | Alere San Diego, Inc. | Universal hairpin probes |
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| WO1991009944A2 (en) * | 1989-12-22 | 1991-07-11 | F.Hoffmann-La Roche Ag | High temperature reverse transcriptases |
| US5455166A (en) | 1991-01-31 | 1995-10-03 | Becton, Dickinson And Company | Strand displacement amplification |
| US5556751A (en) | 1991-04-25 | 1996-09-17 | Amoco Corporation | Selective amplification system using Q-β replicase |
| US5834202A (en) | 1992-08-04 | 1998-11-10 | Replicon, Inc. | Methods for the isothermal amplification of nucleic acid molecules |
| WO1994003624A1 (en) | 1992-08-04 | 1994-02-17 | Auerbach Jeffrey I | Methods for the isothermal amplification of nucleic acid molecules |
| US5733733A (en) | 1992-08-04 | 1998-03-31 | Replicon, Inc. | Methods for the isothermal amplification of nucleic acid molecules |
| US5614389A (en) | 1992-08-04 | 1997-03-25 | Replicon, Inc. | Methods for the isothermal amplification of nucleic acid molecules |
| US5648211A (en) | 1994-04-18 | 1997-07-15 | Becton, Dickinson And Company | Strand displacement amplification using thermophilic enzymes |
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| US6197557B1 (en) * | 1997-03-05 | 2001-03-06 | The Regents Of The University Of Michigan | Compositions and methods for analysis of nucleic acids |
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| EP2464738A4 (en) * | 2009-08-12 | 2013-05-01 | Nugen Technologies Inc | METHODS, COMPOSITIONS, AND KITS FOR GENERATING NUCLEIC ACID PRODUCTS SUBSTANTIALLY FREE OF MATRIX NUCLEIC ACID |
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| KR101870311B1 (ko) | 2012-03-09 | 2018-06-25 | (주)바이오니아 | 핫스타트 역전사반응 또는 핫스타트 역전사 중합효소 연쇄반응용 조성물 |
| CN114214394A (zh) | 2012-06-08 | 2022-03-22 | 爱奥尼安技术公司 | 核苷酸扩增反应 |
| EP3099810B1 (en) | 2014-01-31 | 2019-05-01 | Qiagen GmbH | Cation chelator hot start |
| WO2016004333A2 (en) | 2014-07-02 | 2016-01-07 | Promega Corporation | Reversible metal ion chelators |
-
2013
- 2013-06-07 CN CN202111168266.3A patent/CN114214394A/zh active Pending
- 2013-06-07 CA CA3096342A patent/CA3096342C/en active Active
- 2013-06-07 AU AU2013271404A patent/AU2013271404B2/en active Active
- 2013-06-07 WO PCT/US2013/044796 patent/WO2013185081A1/en not_active Ceased
- 2013-06-07 JP JP2015516255A patent/JP6861465B2/ja active Active
- 2013-06-07 CA CA2876159A patent/CA2876159C/en active Active
- 2013-06-07 CN CN201380029231.9A patent/CN104662159B/zh active Active
- 2013-06-07 EP EP20184582.3A patent/EP3778915A1/en active Pending
- 2013-06-07 US US13/913,153 patent/US10927393B2/en active Active
- 2013-06-07 ES ES13799829T patent/ES2823551T3/es active Active
- 2013-06-07 EP EP13799829.0A patent/EP2859111B1/en active Active
-
2017
- 2017-09-15 AU AU2017228698A patent/AU2017228698B2/en active Active
-
2019
- 2019-02-04 JP JP2019017903A patent/JP2019115344A/ja active Pending
- 2019-09-27 AU AU2019236751A patent/AU2019236751A1/en not_active Abandoned
-
2020
- 2020-12-03 US US17/110,714 patent/US20210155965A1/en not_active Abandoned
-
2021
- 2021-01-29 JP JP2021012803A patent/JP2021072833A/ja active Pending
-
2022
- 2022-02-07 AU AU2022200787A patent/AU2022200787B2/en active Active
-
2024
- 2024-05-21 JP JP2024082357A patent/JP2024109739A/ja active Pending
-
2025
- 2025-01-07 US US19/012,325 patent/US20250277247A1/en active Pending
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