CN107075585A8 - 具有锁核酸的序列转换和信号扩增dna以及使用其的检测方法 - Google Patents

具有锁核酸的序列转换和信号扩增dna以及使用其的检测方法 Download PDF

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CN107075585A8
CN107075585A8 CN201580056020.3A CN201580056020A CN107075585A8 CN 107075585 A8 CN107075585 A8 CN 107075585A8 CN 201580056020 A CN201580056020 A CN 201580056020A CN 107075585 A8 CN107075585 A8 CN 107075585A8
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amplification
nucleic acid
lock nucleic
detection method
sequence conversion
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CN107075585B (zh
CN107075585A (zh
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小宫健
小森诚
吉村徹
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Abbott Laboratories
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Tokyo Institute of Technology NUC
Abbott Japan Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)

Abstract

公开了检测样品中靶核酸的方法。所述方法包括在聚合酶和核酸内切酶的存在下使所述样品与在足以减少非特异性背景信号扩增的选择位置处具有锁核酸的序列转换寡核苷酸接触。还公开了检测样品中靶核酸的方法，其中在聚合酶和核酸内切酶的存在下使所述样品与序列转换寡核苷酸和信号扩增寡核苷酸接触，序列转换寡核苷酸和信号扩增寡核苷酸二者均在足以减少非特异性背景信号扩增的选择位置处具有锁核酸。本文还提供了包含这种序列转换和信号扩增寡核苷酸的组合物和试剂盒。
CN201580056020.3A 2014-10-14 2015-10-13 具有锁核酸的序列转换和信号扩增dna以及使用其的检测方法 Active CN107075585B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201462063666P 2014-10-14 2014-10-14
US62/063,666 2014-10-14
US201462098066P 2014-12-30 2014-12-30
US62/098,066 2014-12-30
PCT/IB2015/002141 WO2016059473A2 (en) 2014-10-14 2015-10-13 Sequence conversion and signal amplifier dna having locked nucleic acids and detection methods using same

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CN107075585A CN107075585A (zh) 2017-08-18
CN107075585A8 true CN107075585A8 (zh) 2019-02-15
CN107075585B CN107075585B (zh) 2021-07-20

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CN201580056020.3A Active CN107075585B (zh) 2014-10-14 2015-10-13 具有锁核酸的序列转换和信号扩增dna以及使用其的检测方法

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US (2) US10316353B2 (zh)
EP (2) EP3207159B1 (zh)
JP (2) JP6896620B2 (zh)
CN (2) CN107109473B (zh)
ES (2) ES2759339T3 (zh)
WO (2) WO2016059473A2 (zh)

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US9845495B2 (en) 2010-12-10 2017-12-19 Abbott Laboratories Method and kit for detecting target nucleic acid
CA2936157C (en) 2014-01-15 2021-08-24 Tokyo Institute Of Technology Covered sequence conversion dna and detection methods
CN107109473B (zh) 2014-10-14 2020-11-06 雅培实验室 具有多dna间隔序列的序列转换和信号扩增dna及使用其的检测方法
ES2791280T3 (es) 2014-12-24 2020-11-03 Abbott Lab Método de detección, composición y kit basados en reacciones en cascada de ADN amplificador de señal con extensión de la diana
GB201611469D0 (en) 2016-06-30 2016-08-17 Lumiradx Tech Ltd Improvements in or relating to nucleic acid amplification processes
GB2569965A (en) 2018-01-04 2019-07-10 Lumiradx Uk Ltd Improvements in or relating to amplification of nucleic acids
WO2021252733A2 (en) * 2020-06-10 2021-12-16 Bonadea Diagnostics, Llc Sequence conversion reaction
WO2023107713A2 (en) * 2021-12-09 2023-06-15 Bonadea Diagnostics, Llc Sequence conversion reaction

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Publication number Publication date
WO2016059474A2 (en) 2016-04-21
JP2017532044A (ja) 2017-11-02
US10208333B2 (en) 2019-02-19
WO2016059473A2 (en) 2016-04-21
EP3207160B1 (en) 2019-11-20
ES2759337T3 (es) 2020-05-08
JP2017530715A (ja) 2017-10-19
WO2016059473A3 (en) 2016-06-23
EP3207159A4 (en) 2018-05-30
EP3207160A4 (en) 2018-05-23
EP3207159A2 (en) 2017-08-23
CN107109473A (zh) 2017-08-29
WO2016059474A3 (en) 2016-06-09
US20160102345A1 (en) 2016-04-14
US20160102339A1 (en) 2016-04-14
CN107075585B (zh) 2021-07-20
CN107075585A (zh) 2017-08-18
CN107109473B (zh) 2020-11-06
JP6896620B2 (ja) 2021-06-30
EP3207160A2 (en) 2017-08-23
US10316353B2 (en) 2019-06-11
JP6773651B2 (ja) 2020-10-21
EP3207159B1 (en) 2019-11-20
ES2759339T3 (es) 2020-05-08

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