JP2013530705A5 - - Google Patents
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- Publication number
- JP2013530705A5 JP2013530705A5 JP2013516753A JP2013516753A JP2013530705A5 JP 2013530705 A5 JP2013530705 A5 JP 2013530705A5 JP 2013516753 A JP2013516753 A JP 2013516753A JP 2013516753 A JP2013516753 A JP 2013516753A JP 2013530705 A5 JP2013530705 A5 JP 2013530705A5
- Authority
- JP
- Japan
- Prior art keywords
- probe
- oligonucleotide
- anabolic
- reaction mixture
- oligonucleotide strand
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108091034117 Oligonucleotide Proteins 0.000 claims 26
- 239000000523 sample Substances 0.000 claims 21
- 238000007397 LAMP assay Methods 0.000 claims 16
- 238000000034 method Methods 0.000 claims 16
- 108020004414 DNA Proteins 0.000 claims 13
- 230000001195 anabolic effect Effects 0.000 claims 12
- 239000011541 reaction mixture Substances 0.000 claims 9
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 7
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 7
- WCKQPPQRFNHPRJ-UHFFFAOYSA-N 4-[[4-(dimethylamino)phenyl]diazenyl]benzoic acid Chemical compound C1=CC(N(C)C)=CC=C1N=NC1=CC=C(C(O)=O)C=C1 WCKQPPQRFNHPRJ-UHFFFAOYSA-N 0.000 claims 2
- 230000001580 bacterial effect Effects 0.000 claims 2
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 claims 2
- 238000012544 monitoring process Methods 0.000 claims 2
- 239000002096 quantum dot Substances 0.000 claims 2
- ABZLKHKQJHEPAX-UHFFFAOYSA-N tetramethylrhodamine Chemical compound C=12C=CC(N(C)C)=CC2=[O+]C2=CC(N(C)C)=CC=C2C=1C1=CC=CC=C1C([O-])=O ABZLKHKQJHEPAX-UHFFFAOYSA-N 0.000 claims 2
- 241000701959 Escherichia virus Lambda Species 0.000 claims 1
- 241000607142 Salmonella Species 0.000 claims 1
- 241000191967 Staphylococcus aureus Species 0.000 claims 1
- 230000000295 complement effect Effects 0.000 claims 1
- 238000001514 detection method Methods 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US35742810P | 2010-06-22 | 2010-06-22 | |
| US61/357,428 | 2010-06-22 | ||
| PCT/US2011/041540 WO2011163425A1 (en) | 2010-06-22 | 2011-06-22 | Sequence specific real-time monitoring of loop-mediated isothermal amplification (lamp) |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2013530705A JP2013530705A (ja) | 2013-08-01 |
| JP2013530705A5 true JP2013530705A5 (enExample) | 2014-07-31 |
| JP5984802B2 JP5984802B2 (ja) | 2016-09-07 |
Family
ID=45371810
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013516753A Expired - Fee Related JP5984802B2 (ja) | 2010-06-22 | 2011-06-22 | ループ媒介性等温増幅（ｌａｍｐ）の配列特異的実時間モニタリング |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US20130171643A1 (enExample) |
| EP (1) | EP2585617B1 (enExample) |
| JP (1) | JP5984802B2 (enExample) |
| KR (1) | KR101832011B1 (enExample) |
| CN (1) | CN103069009B (enExample) |
| AU (1) | AU2011270885B2 (enExample) |
| BR (1) | BR112012032519A2 (enExample) |
| CA (1) | CA2803161C (enExample) |
| MX (1) | MX2012014917A (enExample) |
| WO (1) | WO2011163425A1 (enExample) |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013065574A1 (ja) | 2011-10-31 | 2013-05-10 | 栄研化学株式会社 | 標的核酸の検出法 |
| US9074249B2 (en) | 2012-06-04 | 2015-07-07 | New England Biolabs, Inc. | Detection of amplification products |
| US9074243B2 (en) | 2012-07-27 | 2015-07-07 | New England Biolabs, Inc. | Detection of amplification products |
| CN103540660B (zh) * | 2013-10-10 | 2015-10-28 | 中国人民解放军疾病预防控制所 | 基于TaqMan探针的环介导恒温扩增法及其专用LAMP引物与试剂盒 |
| CN103602727B (zh) * | 2013-10-25 | 2015-03-11 | 中国林业科学研究院热带林业研究所 | 一种快速检测青枯菌的检测引物组、检测试剂盒和检测方法 |
| US10106837B1 (en) | 2014-02-05 | 2018-10-23 | Steven A Benner | Processes for point of care detection of DNA and RNA |
| KR101496835B1 (ko) | 2014-03-04 | 2015-02-27 | 서울대학교산학협력단 | 살모넬라균 검출을 위한 등온증폭반응용 프라이머 조성물 및 이를 이용한 살모넬라균의 검출 방법 |
| US10913973B2 (en) * | 2014-09-17 | 2021-02-09 | Board Of Regents, The University Texas System | Methods and devices related to toehold-based strand displacement with loop-mediated isothermal amplification |
| US10724091B1 (en) | 2015-02-10 | 2020-07-28 | National Technology & Engineering Solutions Of Sandia, Llc | Endpoint detection of amplified nucleic acids |
| KR101552159B1 (ko) | 2015-03-10 | 2015-09-10 | 경기대학교 산학협력단 | 고리-매개 등온 증폭법을 이용한 진균성 질병 검출 방법 |
| CN104745694A (zh) * | 2015-03-17 | 2015-07-01 | 苏州华麦生物科技有限公司 | 一种转基因水稻科丰6的恒温探针法检测引物组、检测试剂盒及检测方法 |
| CN104946760B (zh) * | 2015-06-16 | 2017-12-08 | 河南省农业科学院 | 一种检测肿囊腐霉的方法及专用试剂盒 |
| KR101875662B1 (ko) * | 2015-12-01 | 2018-08-02 | 에스디 바이오센서 주식회사 | 등온 핵산 증폭을 위한 리포터 염료, 퀀처가 포함되는 등온 기반 이중 기능성 올리고뉴클레오타이드 및 이를 이용한 핵산 증폭과 측정 방법 |
| ITUB20159332A1 (it) | 2015-12-22 | 2017-06-22 | Diasorin S P A | Procedimento di rivelazione in fluorescenza dell?amplificazione isotermica mediata da loop (LAMP) di un acido nucleico bersaglio, relativi oligonucleotidi e kit. |
| US11345954B2 (en) | 2016-08-05 | 2022-05-31 | Arizona Board Of Regents On Behalf Of Arizona State University | Methods for digital readout quantification of nucleic acids |
| EP3290527B1 (en) * | 2016-08-29 | 2019-06-19 | MetaSystems Indigo GmbH | Method for detecting microorganisms in a sample |
| US20210395810A1 (en) * | 2016-11-30 | 2021-12-23 | Steven A Benner | Processes to Detect Coronaviruses |
| US11366116B1 (en) | 2017-04-12 | 2022-06-21 | National Technology & Engineering Solutions Of Sandia, Llc | Real time autonomous surveillance of pathogens |
| GB201713251D0 (en) * | 2017-08-18 | 2017-10-04 | Lgc Genomics Ltd | Methods and kits for detection of nucleic acid molecules |
| SG10201910254YA (en) * | 2019-11-04 | 2021-06-29 | Denka Company Ltd | Nucleic Acid Detection Method Using Lamp And Probe Detection |
| KR102687405B1 (ko) * | 2020-10-16 | 2024-07-23 | 경북대학교 산학협력단 | 돼지 써코바이러스 3형 검출용 조성물 및 이를 이용한 돼지 써코바이러스 3형 검출 방법 |
| KR20240104144A (ko) | 2021-12-10 | 2024-07-04 | 주식회사 씨젠 | 단일 가닥 상호작용적 이중 표지 시스템을 포함하는 태그된 루프프라이머 및 이를 이용한 핵산 등온 증폭 방법 |
| CN114196769B (zh) * | 2021-12-27 | 2024-03-08 | 中国科学院长春应用化学研究所 | 一种用于检测沙门氏菌的引物、试剂盒及其使用方法 |
| EP4545648A1 (en) | 2022-06-27 | 2025-04-30 | Seegene, Inc. | Method for amplifying target nucleic acid using beta-hydroxy acid |
| CN119351619A (zh) * | 2023-08-18 | 2025-01-24 | 福建师范大学 | 一种实时双重环介导等温扩增法检测猴痘病毒的引物探针组合、试剂盒及方法 |
| CN117867152B (zh) * | 2024-03-08 | 2024-05-24 | 云南英茂现代农业有限公司 | 一种用于检测青枯菌的核酸引物组、包含其的产品及应用 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080220425A1 (en) * | 1996-01-24 | 2008-09-11 | Third Wave Technologies, Inc. | Methods and Compositions for Detecting Target Sequences |
| US20080085515A1 (en) * | 2000-03-24 | 2008-04-10 | Eppendorf Array Technologies Sa (Eat) | Identification of multiple biological (micro) organisms by detection of their nucleotide sequences on arrays |
| PT1633888E (pt) * | 2003-06-17 | 2009-04-22 | Keygene Nv | Meios e método para a detecção de sequências de nucleótidos alvo utilizando ensaios de ligação com pares de sondas de oligonucleótido melhoradas |
| FI20031678A0 (fi) * | 2003-11-18 | 2003-11-18 | Teemu Korpimaeki | Näytteen käsittelymenetelmä ja järjestely siihen |
| US20060115838A1 (en) * | 2004-10-19 | 2006-06-01 | Trevigen Inc. | Real-time detection of amplicons using nucleic acid repair enzymes |
| NZ548731A (en) * | 2006-07-24 | 2008-12-24 | Zygem Corp Ltd | Isothermal detection methods and uses thereof |
| JP2009159945A (ja) * | 2007-12-13 | 2009-07-23 | Hitachi High-Technologies Corp | 核酸検出用プローブ |
| WO2009108693A2 (en) * | 2008-02-25 | 2009-09-03 | The Government Of The United States Of America , As Represented By The Secretary, Department Of Health And Human Services, | Composition and methods for rapid detection of hiv by loop- mediated isothermal amplification (lamp) |
-
2011
- 2011-06-22 US US13/805,578 patent/US20130171643A1/en not_active Abandoned
- 2011-06-22 MX MX2012014917A patent/MX2012014917A/es active IP Right Grant
- 2011-06-22 JP JP2013516753A patent/JP5984802B2/ja not_active Expired - Fee Related
- 2011-06-22 CN CN201180040896.0A patent/CN103069009B/zh not_active Expired - Fee Related
- 2011-06-22 BR BR112012032519A patent/BR112012032519A2/pt not_active Application Discontinuation
- 2011-06-22 KR KR1020137001509A patent/KR101832011B1/ko not_active Expired - Fee Related
- 2011-06-22 CA CA2803161A patent/CA2803161C/en not_active Expired - Fee Related
- 2011-06-22 EP EP11798891.5A patent/EP2585617B1/en not_active Not-in-force
- 2011-06-22 AU AU2011270885A patent/AU2011270885B2/en not_active Ceased
- 2011-06-22 WO PCT/US2011/041540 patent/WO2011163425A1/en not_active Ceased
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