JP2014504880A5 - - Google Patents
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- JP2014504880A5 JP2014504880A5 JP2013550902A JP2013550902A JP2014504880A5 JP 2014504880 A5 JP2014504880 A5 JP 2014504880A5 JP 2013550902 A JP2013550902 A JP 2013550902A JP 2013550902 A JP2013550902 A JP 2013550902A JP 2014504880 A5 JP2014504880 A5 JP 2014504880A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- exonuclease activity
- polymerase
- extension product
- extension
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 238000000034 method Methods 0.000 claims 33
- 239000000523 sample Substances 0.000 claims 23
- 108020004707 nucleic acids Proteins 0.000 claims 19
- 102000039446 nucleic acids Human genes 0.000 claims 19
- 150000007523 nucleic acids Chemical class 0.000 claims 18
- 230000000694 effects Effects 0.000 claims 14
- 108091034117 Oligonucleotide Proteins 0.000 claims 11
- 108010068698 spleen exonuclease Proteins 0.000 claims 11
- 239000012491 analyte Substances 0.000 claims 9
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 6
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 6
- 230000003321 amplification Effects 0.000 claims 6
- 238000003199 nucleic acid amplification method Methods 0.000 claims 6
- 108010017826 DNA Polymerase I Proteins 0.000 claims 4
- 102000004594 DNA Polymerase I Human genes 0.000 claims 4
- 125000003729 nucleotide group Chemical group 0.000 claims 4
- 108060002716 Exonuclease Proteins 0.000 claims 3
- 239000003153 chemical reaction reagent Substances 0.000 claims 3
- 102000013165 exonuclease Human genes 0.000 claims 3
- 238000003753 real-time PCR Methods 0.000 claims 3
- 238000005096 rolling process Methods 0.000 claims 3
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 2
- 239000012634 fragment Substances 0.000 claims 2
- 239000002773 nucleotide Substances 0.000 claims 2
- 238000003752 polymerase chain reaction Methods 0.000 claims 2
- 108010071146 DNA Polymerase III Proteins 0.000 claims 1
- 102000007528 DNA Polymerase III Human genes 0.000 claims 1
- 108010007577 Exodeoxyribonuclease I Proteins 0.000 claims 1
- 102100029075 Exonuclease 1 Human genes 0.000 claims 1
- 108091093037 Peptide nucleic acid Proteins 0.000 claims 1
- 108010019653 Pwo polymerase Proteins 0.000 claims 1
- 108010006785 Taq Polymerase Proteins 0.000 claims 1
- 238000004458 analytical method Methods 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 230000000903 blocking effect Effects 0.000 claims 1
- 239000003795 chemical substances by application Substances 0.000 claims 1
- 238000003505 heat denaturation Methods 0.000 claims 1
- 230000002779 inactivation Effects 0.000 claims 1
- 230000003993 interaction Effects 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB1101621.9 | 2011-01-31 | ||
| GBGB1101621.9A GB201101621D0 (en) | 2011-01-31 | 2011-01-31 | Method and product |
| PCT/EP2012/051474 WO2012104261A1 (en) | 2011-01-31 | 2012-01-30 | Exonuclease enabled proximity extension assays |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017019754A Division JP6457564B2 (ja) | 2011-01-31 | 2017-02-06 | エキソヌクレアーゼを用いた近接伸長アッセイ |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2014504880A JP2014504880A (ja) | 2014-02-27 |
| JP2014504880A5 true JP2014504880A5 (enExample) | 2015-03-12 |
| JP6180940B2 JP6180940B2 (ja) | 2017-08-16 |
Family
ID=43824847
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013550902A Active JP6180940B2 (ja) | 2011-01-31 | 2012-01-30 | エキソヌクレアーゼを用いた近接伸長アッセイ |
| JP2017019754A Active JP6457564B2 (ja) | 2011-01-31 | 2017-02-06 | エキソヌクレアーゼを用いた近接伸長アッセイ |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017019754A Active JP6457564B2 (ja) | 2011-01-31 | 2017-02-06 | エキソヌクレアーゼを用いた近接伸長アッセイ |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US9777315B2 (enExample) |
| EP (2) | EP2670860B1 (enExample) |
| JP (2) | JP6180940B2 (enExample) |
| CN (1) | CN103354840B (enExample) |
| GB (1) | GB201101621D0 (enExample) |
| WO (1) | WO2012104261A1 (enExample) |
Families Citing this family (75)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2791655C (en) | 2010-03-01 | 2014-11-04 | Quanterix Corporation | Methods and systems for extending dynamic range in assays for the detection of molecules or particles |
| US9952237B2 (en) | 2011-01-28 | 2018-04-24 | Quanterix Corporation | Systems, devices, and methods for ultra-sensitive detection of molecules or particles |
| GB201101621D0 (en) * | 2011-01-31 | 2011-03-16 | Olink Ab | Method and product |
| GB201201547D0 (en) * | 2012-01-30 | 2012-03-14 | Olink Ab | Method and product |
| KR20230169418A (ko) * | 2013-03-13 | 2023-12-15 | 메소 스케일 테크놀러지즈, 엘엘시 | 개선된 분석 방법 |
| US10114015B2 (en) | 2013-03-13 | 2018-10-30 | Meso Scale Technologies, Llc. | Assay methods |
| RU2015123459A (ru) * | 2013-03-15 | 2017-04-20 | Флуидигм Корпорейшн | Одновременная детекция белка-мишени и нуклеиновых кислот-мишеней в одной клетке |
| IE86624B1 (en) | 2013-05-21 | 2016-02-10 | Src Inc | Methods and systems for quantitative fluorescence-based detection of molecules and proteins |
| CA2922926A1 (en) | 2013-09-04 | 2015-03-12 | Fluidigm Corporation | Proximity assays for detecting nucleic acids and proteins in a single cell |
| GB201401885D0 (en) * | 2014-02-04 | 2014-03-19 | Olink Ab | Proximity assay with detection based on hybridisation chain reaction (HCR) |
| US10112194B2 (en) | 2014-04-14 | 2018-10-30 | Q-Linea Ab | Detection of microscopic objects |
| CN119709943A (zh) | 2014-05-15 | 2025-03-28 | 中尺度技术有限责任公司 | 改进的测定方法 |
| US9909167B2 (en) * | 2014-06-23 | 2018-03-06 | The Board Of Trustees Of The Leland Stanford Junior University | On-slide staining by primer extension |
| GB201414745D0 (en) | 2014-08-19 | 2014-10-01 | Articzymes As | Exonucleases |
| CN105483115A (zh) * | 2014-09-19 | 2016-04-13 | 深圳华大基因科技有限公司 | 一种用于单细胞全基因组扩增的试剂盒及其应用 |
| CN104293930B (zh) * | 2014-09-28 | 2016-07-06 | 南京诺唯赞生物科技有限公司 | 一种3’-5’外切酶活性测定方法 |
| GB201518655D0 (en) | 2015-10-21 | 2015-12-02 | Olink Ab | Method for generating proximity probes |
| CN119574873A (zh) | 2016-05-02 | 2025-03-07 | Encodia公司 | 采用核酸编码的大分子分析 |
| US20170342463A1 (en) * | 2016-05-25 | 2017-11-30 | Bio-Rad Laboratories, Inc. | Digital proximity assay |
| WO2017205719A1 (en) | 2016-05-27 | 2017-11-30 | President And Fellows Of Harvard College | Conditional primer extension for single-molecule detection |
| EP4180533B1 (en) | 2016-07-27 | 2025-08-06 | The Board of Trustees of the Leland Stanford Junior University | Highly-multiplexed fluorescent imaging |
| GB201614023D0 (en) | 2016-08-16 | 2016-09-28 | Olink Bioscience Ab | Double-stranded circle probes |
| WO2018102759A1 (en) | 2016-12-01 | 2018-06-07 | Ignite Biosciences, Inc. | Methods of assaying proteins |
| GB201700567D0 (en) | 2017-01-12 | 2017-03-01 | Genagon Therapeutics Ab | Therapeutic agents |
| KR20200109293A (ko) | 2017-09-13 | 2020-09-22 | 프로제너티, 인크. | 자간전증 바이오마커 및 관련된 시스템 및 방법 |
| CN112272703B (zh) | 2017-10-31 | 2024-12-27 | Encodia公司 | 采用核酸编码和/或标签进行分析的试剂盒 |
| CA3081451A1 (en) | 2017-10-31 | 2019-05-09 | Encodia, Inc. | A method for assaying interactions between biological targets and binding agents |
| KR20250152680A (ko) | 2017-12-29 | 2025-10-23 | 노틸러스 서브시디어리, 인크. | 단백질 식별을 위한 디코딩 접근법 |
| JP7096893B2 (ja) * | 2018-02-05 | 2022-07-06 | エフ．ホフマン－ラ ロシュ アーゲー | 単一分子のための一本鎖環状ｄｎａ鋳型の作製 |
| CN112941155B (zh) * | 2018-05-14 | 2023-07-14 | 北京艾克伦医疗科技有限公司 | 具有茎环结构的dna引物对及其应用 |
| US11629345B2 (en) * | 2018-06-06 | 2023-04-18 | The Regents Of The University Of California | Methods of producing nucleic acid libraries and compositions and kits for practicing same |
| US20210255189A1 (en) | 2018-06-15 | 2021-08-19 | Olink Proteomics Ab | Biomarker panel for ovarian cancer |
| CA3114754A1 (en) * | 2018-12-21 | 2020-06-25 | Scott Kuersten | Nuclease-based rna depletion |
| WO2020146603A1 (en) * | 2019-01-09 | 2020-07-16 | Qiagen Sciences, Llc | Methods of detecting analytes and compositions thereof |
| WO2020180741A1 (en) | 2019-03-01 | 2020-09-10 | Mercy Bioanalytics, Inc. | Systems, compositions, and methods for target entity detection |
| WO2020220013A1 (en) * | 2019-04-26 | 2020-10-29 | Arizona Board Of Regents On Behalf Of Arizona State University | Proximity-driven activation of crispr-cas systems for detection of diverse molecular analytes |
| CA3138367A1 (en) | 2019-04-30 | 2020-11-05 | Encodia, Inc. | Methods for preparing analytes and related kits |
| CN115066434A (zh) | 2019-12-03 | 2022-09-16 | 阿拉玛生物科学公司 | 核酸连接的免疫-夹心测定(nulisa) |
| EP4070113A4 (en) | 2019-12-04 | 2023-12-20 | Biora Therapeutics, Inc. | ASSESSMENT OF PREECAMPSIA USING FREE AND DISSOCIATE PLACENTAL GROWTH FACTOR ASSAYS |
| GB202004469D0 (en) * | 2020-03-27 | 2020-05-13 | Olink Proteomics Ab | Controls for proximity detection assays |
| WO2021252800A1 (en) | 2020-06-11 | 2021-12-16 | Nautilus Biotechnology, Inc. | Methods and systems for computational decoding of biological, chemical, and physical entities |
| JP2023545038A (ja) * | 2020-10-06 | 2023-10-26 | プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ | 中和標的のシグナルオン検出のための方法、組成物、キット、およびシステム |
| GB202018503D0 (en) | 2020-11-25 | 2021-01-06 | Olink Proteomics Ab | Analyte detection method employing concatamers |
| KR102573998B1 (ko) * | 2020-12-30 | 2023-09-04 | 주식회사 스타노스 | 위양성 신호가 제거된 항원 검출방법 및 키트 |
| CA3203106A1 (en) | 2021-01-20 | 2022-07-28 | Parag Mallick | Systems and methods for biomolecule quantitation |
| EP4281775A1 (en) | 2021-01-21 | 2023-11-29 | Nautilus Subsidiary, Inc. | Systems and methods for biomolecule preparation |
| IL305336B2 (en) | 2021-03-11 | 2025-09-01 | Nautilus Subsidiary Inc | Systems and methods for preserving biomolecules |
| US20230035685A1 (en) * | 2021-07-30 | 2023-02-02 | 10X Genomics, Inc. | One step in situ rolling circle amplification assay |
| CA3227872A1 (en) | 2021-09-09 | 2023-03-16 | James Henry JOLY | Characterization and localization of protein modifications |
| WO2023049073A1 (en) | 2021-09-22 | 2023-03-30 | Nautilus Biotechnology, Inc. | Methods and systems for determining polypeptide interactions |
| WO2023081728A1 (en) | 2021-11-03 | 2023-05-11 | Nautilus Biotechnology, Inc. | Systems and methods for surface structuring |
| EP4500157A1 (en) | 2022-03-29 | 2025-02-05 | Nautilus Subsidiary, Inc. | Integrated arrays for single-analyte processes |
| WO2023212490A1 (en) | 2022-04-25 | 2023-11-02 | Nautilus Subsidiary, Inc. | Systems and methods for assessing and improving the quality of multiplex molecular assays |
| EP4544077A1 (en) | 2022-06-21 | 2025-04-30 | Nautilus Subsidiary, Inc. | Method for detecting analytes at sites of optically non-resolvable distances |
| WO2024059655A1 (en) | 2022-09-15 | 2024-03-21 | Nautilus Subsidiary, Inc. | Characterizing accessibility of macromolecule structures |
| WO2024073599A1 (en) | 2022-09-29 | 2024-04-04 | Nautilus Subsidiary, Inc. | Preparation of array surfaces for single-analyte processes |
| EP4382534A1 (en) | 2022-12-06 | 2024-06-12 | Biomérieux | Synthetic peptide for use in an immunomolecular assay |
| WO2024124073A1 (en) | 2022-12-09 | 2024-06-13 | Nautilus Subsidiary, Inc. | A method comprising performing on a single-analyte array at least 50 cycles of a process |
| EP4634285A1 (en) | 2022-12-15 | 2025-10-22 | Nautilus Subsidiary, Inc. | Inhibition of photon phenomena on single molecule arrays |
| EP4655590A1 (en) | 2023-01-27 | 2025-12-03 | Biomerieux | Modified immuno-molecular assay for faster biomarker detection |
| US20240344116A1 (en) | 2023-03-24 | 2024-10-17 | Nautilus Subsidiary, Inc. | Transfer of nanoparticles to array surfaces |
| US20240394902A1 (en) | 2023-05-24 | 2024-11-28 | Nautilus Subsidiary, Inc. | Systems and methods for machine vision |
| WO2025012398A1 (en) | 2023-07-11 | 2025-01-16 | Olink Proteomics Ab | Dried reagents |
| WO2025043159A1 (en) | 2023-08-24 | 2025-02-27 | Nautilus Subsidiary, Inc. | Compositions and methods for detecting binding interactions under equilibrium or non-equilibrium conditions |
| WO2025111397A1 (en) | 2023-11-20 | 2025-05-30 | Alamar Biosciences, Inc. | Use of autoinhibition standard curves in proximity assays |
| WO2025111396A1 (en) | 2023-11-20 | 2025-05-30 | Alamar Biosciences, Inc. | Molecular barcode set and use thereof in multiplex proximity detection assays |
| WO2025111398A1 (en) | 2023-11-20 | 2025-05-30 | Alamar Biosciences, Inc. | Reduction of non-cognate signal in multiplex proximity ligation assays |
| CN117343999B (zh) * | 2023-12-05 | 2024-03-01 | 上海锐赛循益生物技术有限公司 | 基于左侧探针退火和右侧探针退火延伸的核酸扩增方法 |
| US20250189519A1 (en) | 2023-12-11 | 2025-06-12 | Nautilus Subsidiary, Inc. | Methods and compositions of particle-based arrays |
| WO2025137166A1 (en) | 2023-12-20 | 2025-06-26 | Nautilus Subsidiary, Inc. | Fluidic devices for single-analyte assays |
| WO2025168224A1 (en) | 2024-02-08 | 2025-08-14 | Leica Microsystems Cms Gmbh | Marker and method for analysing a biological sample |
| WO2025199005A1 (en) | 2024-03-20 | 2025-09-25 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Biomarkers for identifying cancer patients at high risk for chemotherapy-induced cardiotoxicity |
| US20250298016A1 (en) | 2024-03-22 | 2025-09-25 | Nautilus Subsidiary, Inc. | Plasmonic particle systems for single-analyte assays |
| US20250305034A1 (en) | 2024-04-01 | 2025-10-02 | Nautilus Subsidiary, Inc. | Nanostructures for modulation of analyte conformation |
| WO2025262178A1 (en) * | 2024-06-18 | 2025-12-26 | Olink Proteomics Ab | System and method for detecting analytes in a sample |
Family Cites Families (65)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU530410B2 (en) | 1978-02-21 | 1983-07-14 | Sintef | Preparing aqueous emulsions |
| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4883867A (en) | 1985-11-01 | 1989-11-28 | Becton, Dickinson And Company | Detection of reticulocytes, RNA or DNA |
| US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
| US5266684A (en) | 1988-05-02 | 1993-11-30 | The Reagents Of The University Of California | Peptide mixtures |
| US5223409A (en) | 1988-09-02 | 1993-06-29 | Protein Engineering Corp. | Directed evolution of novel binding proteins |
| US5449603A (en) | 1989-10-24 | 1995-09-12 | Stratagene | Method for hybridizing nucleic acids using single-stranded nucleic acid binding protein |
| US5733523A (en) | 1990-12-10 | 1998-03-31 | Akzo Nobel N.V. | Targeted delivery of a therapeutic entity using complementary oligonucleotides |
| JP3403405B2 (ja) | 1991-09-13 | 2003-05-06 | サイトセル・リミテッド | 核酸伸長検定 |
| EP0605655B1 (en) | 1991-09-16 | 1997-05-07 | Molecular Probes, Inc. | Dimers of unsymmetrical cyanine dyes |
| US5639603A (en) | 1991-09-18 | 1997-06-17 | Affymax Technologies N.V. | Synthesizing and screening molecular diversity |
| DE69217497T2 (de) | 1991-09-18 | 1997-06-12 | Affymax Tech Nv | Verfahren zur synthese der verschiedenen sammlungen von oligomeren |
| WO1993015226A1 (en) | 1992-01-28 | 1993-08-05 | President And Fellows Of Harvard College | Method for nucleic acid hybridization using single-stranded dna binding protein |
| US5321130A (en) | 1992-02-10 | 1994-06-14 | Molecular Probes, Inc. | Unsymmetrical cyanine dyes with a cationic side chain |
| US5541061A (en) | 1992-04-29 | 1996-07-30 | Affymax Technologies N.V. | Methods for screening factorial chemical libraries |
| ATE348175T1 (de) | 1992-07-17 | 2007-01-15 | Dana Farber Cancer Inst Inc | Method eder intrazellularen bindung von zielgerichteten molekülen |
| US5288514A (en) | 1992-09-14 | 1994-02-22 | The Regents Of The University Of California | Solid phase and combinatorial synthesis of benzodiazepine compounds on a solid support |
| US5721099A (en) | 1992-10-01 | 1998-02-24 | Trustees Of Columbia University In The City Of New York | Complex combinatorial chemical libraries encoded with tags |
| US5565324A (en) | 1992-10-01 | 1996-10-15 | The Trustees Of Columbia University In The City Of New York | Complex combinatorial chemical libraries encoded with tags |
| US5271099A (en) | 1992-11-02 | 1993-12-21 | Lin Ming Huei | Sunshade cap with a double layer visor |
| US5410030A (en) | 1993-04-05 | 1995-04-25 | Molecular Probes, Inc. | Dimers of unsymmetrical cyanine dyes containing pyridinium moieties |
| US5436134A (en) | 1993-04-13 | 1995-07-25 | Molecular Probes, Inc. | Cyclic-substituted unsymmetrical cyanine dyes |
| US5658751A (en) | 1993-04-13 | 1997-08-19 | Molecular Probes, Inc. | Substituted unsymmetrical cyanine dyes with selected permeability |
| US5440016A (en) | 1993-06-18 | 1995-08-08 | Torrey Pines Institute For Molecular Studies | Peptides of the formula (KFmoc) ZZZ and their uses |
| US5851829A (en) | 1993-07-16 | 1998-12-22 | Dana-Farber Cancer Institute | Method of intracellular binding of target molecules |
| US5635602A (en) | 1993-08-13 | 1997-06-03 | The Regents Of The University Of California | Design and synthesis of bispecific DNA-antibody conjugates |
| US5593853A (en) | 1994-02-09 | 1997-01-14 | Martek Corporation | Generation and screening of synthetic drug libraries |
| US5512462A (en) | 1994-02-25 | 1996-04-30 | Hoffmann-La Roche Inc. | Methods and reagents for the polymerase chain reaction amplification of long DNA sequences |
| US5688997A (en) | 1994-05-06 | 1997-11-18 | Pharmacopeia, Inc. | Process for preparing intermediates for a combinatorial dihydrobenzopyran library |
| US5525735A (en) | 1994-06-22 | 1996-06-11 | Affymax Technologies Nv | Methods for synthesizing diverse collections of pyrrolidine compounds |
| US5549974A (en) | 1994-06-23 | 1996-08-27 | Affymax Technologies Nv | Methods for the solid phase synthesis of thiazolidinones, metathiazanones, and derivatives thereof |
| US5463564A (en) | 1994-09-16 | 1995-10-31 | 3-Dimensional Pharmaceuticals, Inc. | System and method of automatically generating chemical compounds with desired properties |
| US5688696A (en) | 1994-12-12 | 1997-11-18 | Selectide Corporation | Combinatorial libraries having a predetermined frequency of each species of test compound |
| SE504798C2 (sv) | 1995-06-16 | 1997-04-28 | Ulf Landegren | Immunanalys och testkit med två reagens som kan tvärbindas om de adsorberats till analyten |
| JPH11509086A (ja) | 1995-06-21 | 1999-08-17 | マーテック・バイオサイエンシズ・コーポレイション | 標識生化学化合物の組合わせライブラリーとその作成方法 |
| US5854033A (en) | 1995-11-21 | 1998-12-29 | Yale University | Rolling circle replication reporter systems |
| US5731423A (en) | 1996-03-21 | 1998-03-24 | Transcell Technologies, Inc. | Process for preparing sulfoxides |
| US6117635A (en) | 1996-07-16 | 2000-09-12 | Intergen Company | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
| GB9703369D0 (en) | 1997-02-18 | 1997-04-09 | Lindqvist Bjorn H | Process |
| CA2291721C (en) | 1997-05-28 | 2011-08-09 | Babraham Institute | Ribosome complexes as selection particles for in vitro display and evolution of proteins |
| ES2290979T3 (es) | 1997-12-15 | 2008-02-16 | Csl Behring Gmbh | Cebador marcado para uso en la deteccion de acidos nucleicos diana. |
| US5989823A (en) | 1998-09-18 | 1999-11-23 | Nexstar Pharmaceuticals, Inc. | Homogeneous detection of a target through nucleic acid ligand-ligand beacon interaction |
| EP1066414B1 (en) | 1998-03-25 | 2009-06-10 | Olink AB | Rolling circle replication of padlock probes |
| GB9812768D0 (en) | 1998-06-13 | 1998-08-12 | Zeneca Ltd | Methods |
| SE516272C2 (sv) | 2000-02-18 | 2001-12-10 | Ulf Landegren | Metoder och kit för analytdetektion mha proximitets-probning |
| US6511809B2 (en) | 2000-06-13 | 2003-01-28 | E. I. Du Pont De Nemours And Company | Method for the detection of an analyte by means of a nucleic acid reporter |
| JP2005509444A (ja) | 2001-11-23 | 2005-04-14 | シモン・フレデリックソン | 多価近接プローブにより近接プロービングするための方法およびキット |
| US7553619B2 (en) | 2002-02-08 | 2009-06-30 | Qiagen Gmbh | Detection method using dissociated rolling circle amplification |
| US20050221349A1 (en) | 2002-05-30 | 2005-10-06 | Stuart Wilson | Methods of detecting target molecules and molecular interactions |
| JP5117719B2 (ja) | 2003-04-18 | 2013-01-16 | ベクトン・ディキンソン・アンド・カンパニー | 免疫増幅 |
| US7914987B2 (en) | 2004-06-14 | 2011-03-29 | The Board Of Trustees Of The Leland Stanford Junior University | Methods and compositions for use in analyte detection using proximity probes |
| ATE480643T1 (de) | 2004-11-03 | 2010-09-15 | Iris Molecular Diagnostics Inc | Homogener nachweis von analyten |
| WO2007005649A2 (en) | 2005-06-30 | 2007-01-11 | Applera Corporation | Proximity probing of target proteins comprising restriction and/or extension field |
| US20080293051A1 (en) | 2005-08-30 | 2008-11-27 | Board Of Regents, The University Of Texas System | proximity ligation assay |
| GB0605584D0 (en) | 2006-03-20 | 2006-04-26 | Olink Ab | Method for analyte detection using proximity probes |
| US20080090238A1 (en) | 2006-10-12 | 2008-04-17 | Dan-Hui Dorothy Yang | Increased sensitivity of proximity ligation assays |
| US7659069B2 (en) | 2007-08-31 | 2010-02-09 | Agilent Technologies, Inc. | Binary signaling assay using a split-polymerase |
| WO2009012220A2 (en) | 2007-07-17 | 2009-01-22 | Agilent Technologies Inc. | Analyte detection assays |
| AU2008308993A1 (en) * | 2007-09-28 | 2009-04-09 | Affomix Corporation | Polynucleotide backbones for complexing proteins |
| WO2009123216A1 (ja) * | 2008-03-31 | 2009-10-08 | 独立行政法人理化学研究所 | 高選択・高効率でｐｃｒ増幅が可能な新規ｄｎａ |
| WO2011100561A1 (en) * | 2010-02-12 | 2011-08-18 | Saint Louis University | Molecular biosensors capable of signal amplification |
| GB201011971D0 (en) | 2010-07-15 | 2010-09-01 | Olink Ab | Methods and product |
| GB201101621D0 (en) * | 2011-01-31 | 2011-03-16 | Olink Ab | Method and product |
-
2011
- 2011-01-31 GB GBGB1101621.9A patent/GB201101621D0/en not_active Ceased
-
2012
- 2012-01-30 EP EP12702018.8A patent/EP2670860B1/en active Active
- 2012-01-30 EP EP17183834.5A patent/EP3323896B1/en active Active
- 2012-01-30 CN CN201280006856.9A patent/CN103354840B/zh active Active
- 2012-01-30 US US13/981,943 patent/US9777315B2/en active Active
- 2012-01-30 JP JP2013550902A patent/JP6180940B2/ja active Active
- 2012-01-30 WO PCT/EP2012/051474 patent/WO2012104261A1/en not_active Ceased
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2017
- 2017-02-06 JP JP2017019754A patent/JP6457564B2/ja active Active
- 2017-09-01 US US15/693,582 patent/US10731206B2/en active Active
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