JP2014504880A5 - - Google Patents
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- JP2014504880A5 JP2014504880A5 JP2013550902A JP2013550902A JP2014504880A5 JP 2014504880 A5 JP2014504880 A5 JP 2014504880A5 JP 2013550902 A JP2013550902 A JP 2013550902A JP 2013550902 A JP2013550902 A JP 2013550902A JP 2014504880 A5 JP2014504880 A5 JP 2014504880A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- exonuclease activity
- polymerase
- extension product
- extension
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 238000000034 method Methods 0.000 claims 33
- 239000000523 sample Substances 0.000 claims 23
- 108020004707 nucleic acids Proteins 0.000 claims 19
- 102000039446 nucleic acids Human genes 0.000 claims 19
- 150000007523 nucleic acids Chemical class 0.000 claims 18
- 230000000694 effects Effects 0.000 claims 14
- 108091034117 Oligonucleotide Proteins 0.000 claims 11
- 108010068698 spleen exonuclease Proteins 0.000 claims 11
- 239000012491 analyte Substances 0.000 claims 9
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 6
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 6
- 230000003321 amplification Effects 0.000 claims 6
- 238000003199 nucleic acid amplification method Methods 0.000 claims 6
- 108010017826 DNA Polymerase I Proteins 0.000 claims 4
- 102000004594 DNA Polymerase I Human genes 0.000 claims 4
- 125000003729 nucleotide group Chemical group 0.000 claims 4
- 108060002716 Exonuclease Proteins 0.000 claims 3
- 239000003153 chemical reaction reagent Substances 0.000 claims 3
- 102000013165 exonuclease Human genes 0.000 claims 3
- 238000003753 real-time PCR Methods 0.000 claims 3
- 238000005096 rolling process Methods 0.000 claims 3
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 2
- 239000012634 fragment Substances 0.000 claims 2
- 239000002773 nucleotide Substances 0.000 claims 2
- 238000003752 polymerase chain reaction Methods 0.000 claims 2
- 108010071146 DNA Polymerase III Proteins 0.000 claims 1
- 102000007528 DNA Polymerase III Human genes 0.000 claims 1
- 108010007577 Exodeoxyribonuclease I Proteins 0.000 claims 1
- 102100029075 Exonuclease 1 Human genes 0.000 claims 1
- 108091093037 Peptide nucleic acid Proteins 0.000 claims 1
- 108010019653 Pwo polymerase Proteins 0.000 claims 1
- 108010006785 Taq Polymerase Proteins 0.000 claims 1
- 238000004458 analytical method Methods 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 230000000903 blocking effect Effects 0.000 claims 1
- 239000003795 chemical substances by application Substances 0.000 claims 1
- 238000003505 heat denaturation Methods 0.000 claims 1
- 230000002779 inactivation Effects 0.000 claims 1
- 230000003993 interaction Effects 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB1101621.9 | 2011-01-31 | ||
| GBGB1101621.9A GB201101621D0 (en) | 2011-01-31 | 2011-01-31 | Method and product |
| PCT/EP2012/051474 WO2012104261A1 (en) | 2011-01-31 | 2012-01-30 | Exonuclease enabled proximity extension assays |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017019754A Division JP6457564B2 (ja) | 2011-01-31 | 2017-02-06 | エキソヌクレアーゼを用いた近接伸長アッセイ |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2014504880A JP2014504880A (ja) | 2014-02-27 |
| JP2014504880A5 true JP2014504880A5 (enExample) | 2015-03-12 |
| JP6180940B2 JP6180940B2 (ja) | 2017-08-16 |
Family
ID=43824847
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013550902A Active JP6180940B2 (ja) | 2011-01-31 | 2012-01-30 | エキソヌクレアーゼを用いた近接伸長アッセイ |
| JP2017019754A Active JP6457564B2 (ja) | 2011-01-31 | 2017-02-06 | エキソヌクレアーゼを用いた近接伸長アッセイ |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017019754A Active JP6457564B2 (ja) | 2011-01-31 | 2017-02-06 | エキソヌクレアーゼを用いた近接伸長アッセイ |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US9777315B2 (enExample) |
| EP (2) | EP2670860B1 (enExample) |
| JP (2) | JP6180940B2 (enExample) |
| CN (1) | CN103354840B (enExample) |
| GB (1) | GB201101621D0 (enExample) |
| WO (1) | WO2012104261A1 (enExample) |
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2011
- 2011-01-31 GB GBGB1101621.9A patent/GB201101621D0/en not_active Ceased
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2012
- 2012-01-30 JP JP2013550902A patent/JP6180940B2/ja active Active
- 2012-01-30 WO PCT/EP2012/051474 patent/WO2012104261A1/en not_active Ceased
- 2012-01-30 EP EP12702018.8A patent/EP2670860B1/en active Active
- 2012-01-30 CN CN201280006856.9A patent/CN103354840B/zh active Active
- 2012-01-30 EP EP17183834.5A patent/EP3323896B1/en active Active
- 2012-01-30 US US13/981,943 patent/US9777315B2/en active Active
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