JP2019502374A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2019502374A5 JP2019502374A5 JP2018529612A JP2018529612A JP2019502374A5 JP 2019502374 A5 JP2019502374 A5 JP 2019502374A5 JP 2018529612 A JP2018529612 A JP 2018529612A JP 2018529612 A JP2018529612 A JP 2018529612A JP 2019502374 A5 JP2019502374 A5 JP 2019502374A5
- Authority
- JP
- Japan
- Prior art keywords
- amplification product
- sense strand
- antisense strand
- pair
- absence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000003321 amplification Effects 0.000 claims 20
- 238000003199 nucleic acid amplification method Methods 0.000 claims 20
- 108091081021 Sense strand Proteins 0.000 claims 14
- 230000000692 anti-sense effect Effects 0.000 claims 14
- 239000000523 sample Substances 0.000 claims 13
- 108020004707 nucleic acids Proteins 0.000 claims 12
- 102000039446 nucleic acids Human genes 0.000 claims 12
- 150000007523 nucleic acids Chemical class 0.000 claims 12
- 238000000034 method Methods 0.000 claims 10
- 125000003729 nucleotide group Chemical group 0.000 claims 6
- 239000002773 nucleotide Substances 0.000 claims 5
- 230000000295 complement effect Effects 0.000 claims 3
- 238000000137 annealing Methods 0.000 claims 2
- 238000002866 fluorescence resonance energy transfer Methods 0.000 claims 2
- 238000009396 hybridization Methods 0.000 claims 2
- 230000037452 priming Effects 0.000 claims 2
- 108010037497 3'-nucleotidase Proteins 0.000 claims 1
- 108020004414 DNA Proteins 0.000 claims 1
- 102000053602 DNA Human genes 0.000 claims 1
- 230000004568 DNA-binding Effects 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 claims 1
- 235000010469 Glycine max Nutrition 0.000 claims 1
- 244000068988 Glycine max Species 0.000 claims 1
- 108091034117 Oligonucleotide Proteins 0.000 claims 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- 230000003993 interaction Effects 0.000 claims 1
- 238000011880 melting curve analysis Methods 0.000 claims 1
- 239000003068 molecular probe Substances 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US14/965,343 US10119161B2 (en) | 2015-12-10 | 2015-12-10 | Methods and kits for joining fragmented nucleic acids together |
| US14/965,343 | 2015-12-10 | ||
| PCT/EP2016/080225 WO2017097887A1 (en) | 2015-12-10 | 2016-12-08 | Methods and kits for joining fragmented nucleic acids together |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2019502374A JP2019502374A (ja) | 2019-01-31 |
| JP2019502374A5 true JP2019502374A5 (enExample) | 2020-01-16 |
| JP6986015B2 JP6986015B2 (ja) | 2021-12-22 |
Family
ID=57517894
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018529612A Active JP6986015B2 (ja) | 2015-12-10 | 2016-12-08 | 断片化核酸を連結するための方法及びキット |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US10119161B2 (enExample) |
| EP (1) | EP3387146B1 (enExample) |
| JP (1) | JP6986015B2 (enExample) |
| CN (1) | CN108291255B (enExample) |
| CA (1) | CA3007447C (enExample) |
| ES (1) | ES2886602T3 (enExample) |
| WO (1) | WO2017097887A1 (enExample) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019200161A1 (en) * | 2018-04-11 | 2019-10-17 | Design Therapeutics Inc. | Methods and compounds for the treatment of genetic disease |
| EP3781160A1 (en) * | 2018-04-20 | 2021-02-24 | Design Therapeutics, Inc. | Methods and compounds for the treatment of genetic disease |
| JP7077992B2 (ja) * | 2019-02-22 | 2022-05-31 | 横河電機株式会社 | 核酸配列計測用デバイス、核酸配列計測方法、および核酸配列計測装置 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE69837711T2 (de) * | 1997-09-29 | 2008-01-10 | City Of Hope, Duarte | Leistungstarke verknüpfung von nukleinsäuresegmenten |
| CN100569956C (zh) * | 2006-09-11 | 2009-12-16 | 中山大学达安基因股份有限公司 | 检测结核分枝杆菌及其耐药基因突变的方法和试剂盒 |
| CN102242211A (zh) * | 2011-07-08 | 2011-11-16 | 无锡锐奇基因生物科技有限公司 | 检测突变核酸序列的方法及试剂盒 |
| ITMI20112177A1 (it) * | 2011-11-29 | 2013-05-30 | Genefast S R L | Metodo per rilevare la sintesi e/o l'amplificazione di un acido nucleico |
| CN103255227B (zh) * | 2013-05-30 | 2015-02-25 | 上海快灵生物科技有限公司 | 引物介导环化的恒温核酸滚环扩增方法及试剂盒 |
-
2015
- 2015-12-10 US US14/965,343 patent/US10119161B2/en active Active
-
2016
- 2016-12-08 EP EP16808648.6A patent/EP3387146B1/en active Active
- 2016-12-08 CA CA3007447A patent/CA3007447C/en active Active
- 2016-12-08 CN CN201680069953.0A patent/CN108291255B/zh active Active
- 2016-12-08 WO PCT/EP2016/080225 patent/WO2017097887A1/en not_active Ceased
- 2016-12-08 ES ES16808648T patent/ES2886602T3/es active Active
- 2016-12-08 JP JP2018529612A patent/JP6986015B2/ja active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Zhao et al. | Isothermal amplification of nucleic acids | |
| JP6480511B2 (ja) | 試料中の核酸配列を定量するための組成物および方法 | |
| US11371082B2 (en) | Cleavable hairpin primers | |
| JP2015156872A5 (enExample) | ||
| US20210254139A1 (en) | Probe detection of loop-mediated amplification products | |
| JP2013509871A5 (enExample) | ||
| CN103717752B (zh) | 核酸分析法 | |
| KR20120084320A (ko) | Ｔｈｄ 프라이머 타겟 검출 | |
| KR102397357B1 (ko) | 포스포로티오에이트 dna로 수식된 헤어핀 프로브 기반의 등온 핵산증폭기술을 이용한 표적핵산 검출방법 | |
| JP2023518217A (ja) | 標的核酸を検出するためのループプライマー及びループ・デ・ループ方法 | |
| JP6707072B2 (ja) | 核酸増幅のための核酸 | |
| JP6181742B2 (ja) | Ｈｅｖアッセイ | |
| JP2019502374A5 (enExample) | ||
| US11713489B2 (en) | Methods for temperature-mediated nested polymerase chain reaction | |
| JP2014533502A5 (enExample) | ||
| KR20160097193A (ko) | 다중 프로브 | |
| JP6239926B2 (ja) | Ｈｃｖ検出のための二重プローブアッセイ | |
| JP2025523984A (ja) | Ｒｎａ技術のシグナル媒介増幅及びｒｎａアプタマーを用いた核酸検出方法 | |
| CN109477139A (zh) | 使用长ssDNA多核苷酸作为PCR测定中的引物的方法 | |
| CN108026569B (zh) | 用于催化性测定的方法和组合物 | |
| JP2020178681A (ja) | 核酸検出方法、及び試薬キット | |
| JP2009533063A5 (enExample) | ||
| JP2014093996A (ja) | 核酸等温増幅方法 | |
| 홍채선 | Fluorometric detection of mutant RNA and microRNA using graphene oxide | |
| KR20240058024A (ko) | 대장균의 o115 혈청형 신속 동정용 프라이머 세트 및 이를 이용한 대장균의 혈청형 동정 방법 |