JP2019502374A5 - - Google Patents
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- Publication number
- JP2019502374A5 JP2019502374A5 JP2018529612A JP2018529612A JP2019502374A5 JP 2019502374 A5 JP2019502374 A5 JP 2019502374A5 JP 2018529612 A JP2018529612 A JP 2018529612A JP 2018529612 A JP2018529612 A JP 2018529612A JP 2019502374 A5 JP2019502374 A5 JP 2019502374A5
- Authority
- JP
- Japan
- Prior art keywords
- amplification product
- sense strand
- antisense strand
- pair
- absence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000003321 amplification Effects 0.000 claims 20
- 238000003199 nucleic acid amplification method Methods 0.000 claims 20
- 108091081021 Sense strand Proteins 0.000 claims 14
- 230000000692 anti-sense effect Effects 0.000 claims 14
- 239000000523 sample Substances 0.000 claims 13
- 108020004707 nucleic acids Proteins 0.000 claims 12
- 102000039446 nucleic acids Human genes 0.000 claims 12
- 150000007523 nucleic acids Chemical class 0.000 claims 12
- 238000000034 method Methods 0.000 claims 10
- 125000003729 nucleotide group Chemical group 0.000 claims 6
- 239000002773 nucleotide Substances 0.000 claims 5
- 230000000295 complement effect Effects 0.000 claims 3
- 238000000137 annealing Methods 0.000 claims 2
- 238000002866 fluorescence resonance energy transfer Methods 0.000 claims 2
- 238000009396 hybridization Methods 0.000 claims 2
- 230000037452 priming Effects 0.000 claims 2
- 108010037497 3'-nucleotidase Proteins 0.000 claims 1
- 108020004414 DNA Proteins 0.000 claims 1
- 102000053602 DNA Human genes 0.000 claims 1
- 230000004568 DNA-binding Effects 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 claims 1
- 235000010469 Glycine max Nutrition 0.000 claims 1
- 244000068988 Glycine max Species 0.000 claims 1
- 108091034117 Oligonucleotide Proteins 0.000 claims 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- 230000003993 interaction Effects 0.000 claims 1
- 238000011880 melting curve analysis Methods 0.000 claims 1
- 239000003068 molecular probe Substances 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US14/965,343 | 2015-12-10 | ||
| US14/965,343 US10119161B2 (en) | 2015-12-10 | 2015-12-10 | Methods and kits for joining fragmented nucleic acids together |
| PCT/EP2016/080225 WO2017097887A1 (en) | 2015-12-10 | 2016-12-08 | Methods and kits for joining fragmented nucleic acids together |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2019502374A JP2019502374A (ja) | 2019-01-31 |
| JP2019502374A5 true JP2019502374A5 (enExample) | 2020-01-16 |
| JP6986015B2 JP6986015B2 (ja) | 2021-12-22 |
Family
ID=57517894
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018529612A Active JP6986015B2 (ja) | 2015-12-10 | 2016-12-08 | 断片化核酸を連結するための方法及びキット |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US10119161B2 (enExample) |
| EP (1) | EP3387146B1 (enExample) |
| JP (1) | JP6986015B2 (enExample) |
| CN (1) | CN108291255B (enExample) |
| CA (1) | CA3007447C (enExample) |
| ES (1) | ES2886602T3 (enExample) |
| WO (1) | WO2017097887A1 (enExample) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP7077992B2 (ja) * | 2019-02-22 | 2022-05-31 | 横河電機株式会社 | 核酸配列計測用デバイス、核酸配列計測方法、および核酸配列計測装置 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE69837711T2 (de) * | 1997-09-29 | 2008-01-10 | City Of Hope, Duarte | Leistungstarke verknüpfung von nukleinsäuresegmenten |
| CN100569956C (zh) * | 2006-09-11 | 2009-12-16 | 中山大学达安基因股份有限公司 | 检测结核分枝杆菌及其耐药基因突变的方法和试剂盒 |
| CN102242211A (zh) * | 2011-07-08 | 2011-11-16 | 无锡锐奇基因生物科技有限公司 | 检测突变核酸序列的方法及试剂盒 |
| ITMI20112177A1 (it) * | 2011-11-29 | 2013-05-30 | Genefast S R L | Metodo per rilevare la sintesi e/o l'amplificazione di un acido nucleico |
| CN103255227B (zh) * | 2013-05-30 | 2015-02-25 | 上海快灵生物科技有限公司 | 引物介导环化的恒温核酸滚环扩增方法及试剂盒 |
-
2015
- 2015-12-10 US US14/965,343 patent/US10119161B2/en active Active
-
2016
- 2016-12-08 ES ES16808648T patent/ES2886602T3/es active Active
- 2016-12-08 EP EP16808648.6A patent/EP3387146B1/en active Active
- 2016-12-08 JP JP2018529612A patent/JP6986015B2/ja active Active
- 2016-12-08 CN CN201680069953.0A patent/CN108291255B/zh active Active
- 2016-12-08 WO PCT/EP2016/080225 patent/WO2017097887A1/en not_active Ceased
- 2016-12-08 CA CA3007447A patent/CA3007447C/en active Active
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