ES2886602T3 - Procedimientos y kits para unir entre sí ácidos nucleicos fragmentados - Google Patents

Procedimientos y kits para unir entre sí ácidos nucleicos fragmentados Download PDF

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Publication number
ES2886602T3
ES2886602T3 ES16808648T ES16808648T ES2886602T3 ES 2886602 T3 ES2886602 T3 ES 2886602T3 ES 16808648 T ES16808648 T ES 16808648T ES 16808648 T ES16808648 T ES 16808648T ES 2886602 T3 ES2886602 T3 ES 2886602T3
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ES
Spain
Prior art keywords
nucleic acid
amplification product
antisense strand
target nucleic
strand
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES16808648T
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English (en)
Spanish (es)
Inventor
Stephen G Will
Yuker Wang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Roche Diagnostics GmbH
Original Assignee
F Hoffmann La Roche AG
Roche Diagnostics GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG, Roche Diagnostics GmbH filed Critical F Hoffmann La Roche AG
Application granted granted Critical
Publication of ES2886602T3 publication Critical patent/ES2886602T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00Reactions of nucleic acids characterised by
    • C12Q2531/10Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113PCR
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2533/00Reactions characterised by the enzymatic reaction principle used
    • C12Q2533/10Reactions characterised by the enzymatic reaction principle used the purpose being to increase the length of an oligonucleotide strand
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/101Homogeneous assay format, e.g. one pot reaction
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
ES16808648T 2015-12-10 2016-12-08 Procedimientos y kits para unir entre sí ácidos nucleicos fragmentados Active ES2886602T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14/965,343 US10119161B2 (en) 2015-12-10 2015-12-10 Methods and kits for joining fragmented nucleic acids together
PCT/EP2016/080225 WO2017097887A1 (en) 2015-12-10 2016-12-08 Methods and kits for joining fragmented nucleic acids together

Publications (1)

Publication Number Publication Date
ES2886602T3 true ES2886602T3 (es) 2021-12-20

Family

ID=57517894

Family Applications (1)

Application Number Title Priority Date Filing Date
ES16808648T Active ES2886602T3 (es) 2015-12-10 2016-12-08 Procedimientos y kits para unir entre sí ácidos nucleicos fragmentados

Country Status (7)

Country Link
US (1) US10119161B2 (enExample)
EP (1) EP3387146B1 (enExample)
JP (1) JP6986015B2 (enExample)
CN (1) CN108291255B (enExample)
CA (1) CA3007447C (enExample)
ES (1) ES2886602T3 (enExample)
WO (1) WO2017097887A1 (enExample)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7077992B2 (ja) * 2019-02-22 2022-05-31 横河電機株式会社 核酸配列計測用デバイス、核酸配列計測方法、および核酸配列計測装置

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69837711T2 (de) * 1997-09-29 2008-01-10 City Of Hope, Duarte Leistungstarke verknüpfung von nukleinsäuresegmenten
CN100569956C (zh) * 2006-09-11 2009-12-16 中山大学达安基因股份有限公司 检测结核分枝杆菌及其耐药基因突变的方法和试剂盒
CN102242211A (zh) * 2011-07-08 2011-11-16 无锡锐奇基因生物科技有限公司 检测突变核酸序列的方法及试剂盒
ITMI20112177A1 (it) * 2011-11-29 2013-05-30 Genefast S R L Metodo per rilevare la sintesi e/o l'amplificazione di un acido nucleico
CN103255227B (zh) * 2013-05-30 2015-02-25 上海快灵生物科技有限公司 引物介导环化的恒温核酸滚环扩增方法及试剂盒

Also Published As

Publication number Publication date
CN108291255A (zh) 2018-07-17
JP2019502374A (ja) 2019-01-31
WO2017097887A1 (en) 2017-06-15
EP3387146A1 (en) 2018-10-17
CN108291255B (zh) 2022-03-11
CA3007447C (en) 2022-04-19
CA3007447A1 (en) 2017-06-15
JP6986015B2 (ja) 2021-12-22
EP3387146B1 (en) 2021-06-30
US20170166953A1 (en) 2017-06-15
US10119161B2 (en) 2018-11-06

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