CN108291255B - 用于将片段化的核酸连接在一起的方法和试剂盒 - Google Patents

用于将片段化的核酸连接在一起的方法和试剂盒 Download PDF

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CN108291255B
CN108291255B CN201680069953.0A CN201680069953A CN108291255B CN 108291255 B CN108291255 B CN 108291255B CN 201680069953 A CN201680069953 A CN 201680069953A CN 108291255 B CN108291255 B CN 108291255B
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nucleic acid
sample
target nucleic
amplification product
primers
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Chinese (zh)
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CN108291255A (zh
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S.G.威尔
Y.王
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113—PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2533/00—Reactions characterised by the enzymatic reaction principle used
    • C12Q2533/10—Reactions characterised by the enzymatic reaction principle used the purpose being to increase the length of an oligonucleotide strand
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/101—Homogeneous assay format, e.g. one pot reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201680069953.0A 2015-12-10 2016-12-08 用于将片段化的核酸连接在一起的方法和试剂盒 Active CN108291255B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14/965,343 US10119161B2 (en) 2015-12-10 2015-12-10 Methods and kits for joining fragmented nucleic acids together
US14/965343 2015-12-10
PCT/EP2016/080225 WO2017097887A1 (en) 2015-12-10 2016-12-08 Methods and kits for joining fragmented nucleic acids together

Publications (2)

Publication Number Publication Date
CN108291255A CN108291255A (zh) 2018-07-17
CN108291255B true CN108291255B (zh) 2022-03-11

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CN201680069953.0A Active CN108291255B (zh) 2015-12-10 2016-12-08 用于将片段化的核酸连接在一起的方法和试剂盒

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Country Link
US (1) US10119161B2 (enExample)
EP (1) EP3387146B1 (enExample)
JP (1) JP6986015B2 (enExample)
CN (1) CN108291255B (enExample)
CA (1) CA3007447C (enExample)
ES (1) ES2886602T3 (enExample)
WO (1) WO2017097887A1 (enExample)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7077992B2 (ja) * 2019-02-22 2022-05-31 横河電機株式会社 核酸配列計測用デバイス、核酸配列計測方法、および核酸配列計測装置

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999016904A1 (en) * 1997-09-29 1999-04-08 City Of Hope Efficient linking of nucleic acid segments
CN100569956C (zh) * 2006-09-11 2009-12-16 中山大学达安基因股份有限公司 检测结核分枝杆菌及其耐药基因突变的方法和试剂盒
CN102242211A (zh) * 2011-07-08 2011-11-16 无锡锐奇基因生物科技有限公司 检测突变核酸序列的方法及试剂盒
ITMI20112177A1 (it) * 2011-11-29 2013-05-30 Genefast S R L Metodo per rilevare la sintesi e/o l'amplificazione di un acido nucleico
CN103255227B (zh) * 2013-05-30 2015-02-25 上海快灵生物科技有限公司 引物介导环化的恒温核酸滚环扩增方法及试剂盒

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Publication number Publication date
US10119161B2 (en) 2018-11-06
JP2019502374A (ja) 2019-01-31
WO2017097887A1 (en) 2017-06-15
US20170166953A1 (en) 2017-06-15
CA3007447C (en) 2022-04-19
CN108291255A (zh) 2018-07-17
ES2886602T3 (es) 2021-12-20
EP3387146B1 (en) 2021-06-30
EP3387146A1 (en) 2018-10-17
CA3007447A1 (en) 2017-06-15
JP6986015B2 (ja) 2021-12-22

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