CA3007447C - Methods and kits for joining fragmented nucleic acids together - Google Patents
Methods and kits for joining fragmented nucleic acids together Download PDFInfo
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- CA3007447C CA3007447C CA3007447A CA3007447A CA3007447C CA 3007447 C CA3007447 C CA 3007447C CA 3007447 A CA3007447 A CA 3007447A CA 3007447 A CA3007447 A CA 3007447A CA 3007447 C CA3007447 C CA 3007447C
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- 150000002540 isothiocyanates Chemical class 0.000 description 1
- 229910021644 lanthanide ion Inorganic materials 0.000 description 1
- 238000001638 lipofection Methods 0.000 description 1
- 238000011528 liquid biopsy Methods 0.000 description 1
- DLBFLQKQABVKGT-UHFFFAOYSA-L lucifer yellow dye Chemical compound [Li+].[Li+].[O-]S(=O)(=O)C1=CC(C(N(C(=O)NN)C2=O)=O)=C3C2=CC(S([O-])(=O)=O)=CC3=C1N DLBFLQKQABVKGT-UHFFFAOYSA-L 0.000 description 1
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- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
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- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
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- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 1
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- GZCRRIHWUXGPOV-UHFFFAOYSA-N terbium atom Chemical compound [Tb] GZCRRIHWUXGPOV-UHFFFAOYSA-N 0.000 description 1
- ABZLKHKQJHEPAX-UHFFFAOYSA-N tetramethylrhodamine Chemical compound C=12C=CC(N(C)C)=CC2=[O+]C2=CC(N(C)C)=CC=C2C=1C1=CC=CC=C1C([O-])=O ABZLKHKQJHEPAX-UHFFFAOYSA-N 0.000 description 1
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- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
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- 230000003612 virological effect Effects 0.000 description 1
- 238000011179 visual inspection Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2531/00—Reactions of nucleic acids characterised by
- C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
- C12Q2531/113—PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2533/00—Reactions characterised by the enzymatic reaction principle used
- C12Q2533/10—Reactions characterised by the enzymatic reaction principle used the purpose being to increase the length of an oligonucleotide strand
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/101—Homogeneous assay format, e.g. one pot reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
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- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US14/965,343 | 2015-12-10 | ||
| US14/965,343 US10119161B2 (en) | 2015-12-10 | 2015-12-10 | Methods and kits for joining fragmented nucleic acids together |
| PCT/EP2016/080225 WO2017097887A1 (en) | 2015-12-10 | 2016-12-08 | Methods and kits for joining fragmented nucleic acids together |
Publications (2)
| Publication Number | Publication Date |
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| CA3007447A1 CA3007447A1 (en) | 2017-06-15 |
| CA3007447C true CA3007447C (en) | 2022-04-19 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3007447A Active CA3007447C (en) | 2015-12-10 | 2016-12-08 | Methods and kits for joining fragmented nucleic acids together |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US10119161B2 (enExample) |
| EP (1) | EP3387146B1 (enExample) |
| JP (1) | JP6986015B2 (enExample) |
| CN (1) | CN108291255B (enExample) |
| CA (1) | CA3007447C (enExample) |
| ES (1) | ES2886602T3 (enExample) |
| WO (1) | WO2017097887A1 (enExample) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP7077992B2 (ja) * | 2019-02-22 | 2022-05-31 | 横河電機株式会社 | 核酸配列計測用デバイス、核酸配列計測方法、および核酸配列計測装置 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| DE69837711T2 (de) * | 1997-09-29 | 2008-01-10 | City Of Hope, Duarte | Leistungstarke verknüpfung von nukleinsäuresegmenten |
| CN100569956C (zh) * | 2006-09-11 | 2009-12-16 | 中山大学达安基因股份有限公司 | 检测结核分枝杆菌及其耐药基因突变的方法和试剂盒 |
| CN102242211A (zh) * | 2011-07-08 | 2011-11-16 | 无锡锐奇基因生物科技有限公司 | 检测突变核酸序列的方法及试剂盒 |
| ITMI20112177A1 (it) * | 2011-11-29 | 2013-05-30 | Genefast S R L | Metodo per rilevare la sintesi e/o l'amplificazione di un acido nucleico |
| CN103255227B (zh) * | 2013-05-30 | 2015-02-25 | 上海快灵生物科技有限公司 | 引物介导环化的恒温核酸滚环扩增方法及试剂盒 |
-
2015
- 2015-12-10 US US14/965,343 patent/US10119161B2/en active Active
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2016
- 2016-12-08 ES ES16808648T patent/ES2886602T3/es active Active
- 2016-12-08 EP EP16808648.6A patent/EP3387146B1/en active Active
- 2016-12-08 JP JP2018529612A patent/JP6986015B2/ja active Active
- 2016-12-08 CN CN201680069953.0A patent/CN108291255B/zh active Active
- 2016-12-08 WO PCT/EP2016/080225 patent/WO2017097887A1/en not_active Ceased
- 2016-12-08 CA CA3007447A patent/CA3007447C/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN108291255A (zh) | 2018-07-17 |
| JP2019502374A (ja) | 2019-01-31 |
| ES2886602T3 (es) | 2021-12-20 |
| WO2017097887A1 (en) | 2017-06-15 |
| EP3387146A1 (en) | 2018-10-17 |
| CN108291255B (zh) | 2022-03-11 |
| CA3007447A1 (en) | 2017-06-15 |
| JP6986015B2 (ja) | 2021-12-22 |
| EP3387146B1 (en) | 2021-06-30 |
| US20170166953A1 (en) | 2017-06-15 |
| US10119161B2 (en) | 2018-11-06 |
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Effective date: 20210715 |