CN103354840B - 核酸外切酶可实现的邻近延伸分析 - Google Patents
核酸外切酶可实现的邻近延伸分析 Download PDFInfo
- Publication number
- CN103354840B CN103354840B CN201280006856.9A CN201280006856A CN103354840B CN 103354840 B CN103354840 B CN 103354840B CN 201280006856 A CN201280006856 A CN 201280006856A CN 103354840 B CN103354840 B CN 103354840B
- Authority
- CN
- China
- Prior art keywords
- nucleic acid
- analyte
- proximity
- extension
- domain
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6804—Nucleic acid analysis using immunogens
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB1101621.9A GB201101621D0 (en) | 2011-01-31 | 2011-01-31 | Method and product |
| GB1101621.9 | 2011-01-31 | ||
| PCT/EP2012/051474 WO2012104261A1 (en) | 2011-01-31 | 2012-01-30 | Exonuclease enabled proximity extension assays |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103354840A CN103354840A (zh) | 2013-10-16 |
| CN103354840B true CN103354840B (zh) | 2016-06-08 |
Family
ID=43824847
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201280006856.9A Active CN103354840B (zh) | 2011-01-31 | 2012-01-30 | 核酸外切酶可实现的邻近延伸分析 |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US9777315B2 (enExample) |
| EP (2) | EP2670860B1 (enExample) |
| JP (2) | JP6180940B2 (enExample) |
| CN (1) | CN103354840B (enExample) |
| GB (1) | GB201101621D0 (enExample) |
| WO (1) | WO2012104261A1 (enExample) |
Families Citing this family (74)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2542890B1 (en) | 2010-03-01 | 2015-05-06 | Quanterix Corporation | Methods for extending dynamic range in assays for the detection of molecules or particles |
| US9952237B2 (en) | 2011-01-28 | 2018-04-24 | Quanterix Corporation | Systems, devices, and methods for ultra-sensitive detection of molecules or particles |
| GB201101621D0 (en) * | 2011-01-31 | 2011-03-16 | Olink Ab | Method and product |
| GB201201547D0 (en) | 2012-01-30 | 2012-03-14 | Olink Ab | Method and product |
| AU2014248759B2 (en) | 2013-03-13 | 2020-02-27 | Meso Scale Technologies, Llc. | Improved assay methods |
| US10114015B2 (en) | 2013-03-13 | 2018-10-30 | Meso Scale Technologies, Llc. | Assay methods |
| CN105121659A (zh) * | 2013-03-15 | 2015-12-02 | 富鲁达公司 | 单细胞中的靶蛋白和靶核酸的同时检测 |
| TWI553122B (zh) | 2013-05-21 | 2016-10-11 | Ｓｒｃ公司 | 用於分子與蛋白質基於螢光之定量偵測的方法與系統 |
| CA2922926A1 (en) | 2013-09-04 | 2015-03-12 | Fluidigm Corporation | Proximity assays for detecting nucleic acids and proteins in a single cell |
| GB201401885D0 (en) * | 2014-02-04 | 2014-03-19 | Olink Ab | Proximity assay with detection based on hybridisation chain reaction (HCR) |
| US10112194B2 (en) | 2014-04-14 | 2018-10-30 | Q-Linea Ab | Detection of microscopic objects |
| EP3143401A4 (en) | 2014-05-15 | 2017-10-11 | Meso Scale Technologies, LLC | Improved assay methods |
| US9909167B2 (en) | 2014-06-23 | 2018-03-06 | The Board Of Trustees Of The Leland Stanford Junior University | On-slide staining by primer extension |
| GB201414745D0 (en) | 2014-08-19 | 2014-10-01 | Articzymes As | Exonucleases |
| CN105483115A (zh) * | 2014-09-19 | 2016-04-13 | 深圳华大基因科技有限公司 | 一种用于单细胞全基因组扩增的试剂盒及其应用 |
| CN104293930B (zh) * | 2014-09-28 | 2016-07-06 | 南京诺唯赞生物科技有限公司 | 一种3’-5’外切酶活性测定方法 |
| GB201518655D0 (en) * | 2015-10-21 | 2015-12-02 | Olink Ab | Method for generating proximity probes |
| KR102741603B1 (ko) | 2016-05-02 | 2024-12-16 | 엔코디아, 인코포레이티드 | 암호화 핵산을 사용한 거대분자 분석 |
| EP3464633A4 (en) * | 2016-05-25 | 2020-01-01 | Bio-Rad Laboratories, Inc. | DIGITAL PROXIMITY TEST |
| CN109312337B (zh) | 2016-05-27 | 2022-10-04 | 哈佛学院院长及董事 | 用于单分子检测的条件性引物延伸 |
| CN116064727A (zh) | 2016-07-27 | 2023-05-05 | 斯坦福大学托管董事会 | 高度复用荧光成像 |
| GB201614023D0 (en) | 2016-08-16 | 2016-09-28 | Olink Bioscience Ab | Double-stranded circle probes |
| EP3548652B1 (en) | 2016-12-01 | 2024-04-03 | Nautilus Subsidiary, Inc. | Methods of assaying proteins |
| GB201700567D0 (en) | 2017-01-12 | 2017-03-01 | Genagon Therapeutics Ab | Therapeutic agents |
| US20200264188A1 (en) | 2017-09-13 | 2020-08-20 | Progenity, Inc. | Preeclampsia biomarkers and related systems and methods |
| SG11202003923PA (en) | 2017-10-31 | 2020-05-28 | Encodia Inc | Methods and kits using nucleic acid encoding and/or label |
| KR102556494B1 (ko) | 2017-10-31 | 2023-07-18 | 엔코디아, 인코포레이티드 | 핵산 암호화 및/또는 표지를 이용한 분석용 키트 |
| CA3086915A1 (en) | 2017-12-29 | 2019-07-04 | Sujal M. Patel | Decoding approaches for protein identification |
| JP7096893B2 (ja) * | 2018-02-05 | 2022-07-06 | エフ．ホフマン－ラ ロシュ アーゲー | 単一分子のための一本鎖環状ｄｎａ鋳型の作製 |
| CN112941155B (zh) * | 2018-05-14 | 2023-07-14 | 北京艾克伦医疗科技有限公司 | 具有茎环结构的dna引物对及其应用 |
| CN112243462B (zh) * | 2018-06-06 | 2025-02-25 | 加利福尼亚大学董事会 | 产生核酸文库的方法以及用于实践所述方法的组合物和试剂盒 |
| WO2019238944A1 (en) | 2018-06-15 | 2019-12-19 | Olink Proteomics Ab | Biomarker panel for ovarian cancer |
| JP2022512058A (ja) * | 2018-12-21 | 2022-02-02 | イルミナ インコーポレイテッド | ヌクレアーゼを利用したｒｎａ枯渇 |
| CN113302301A (zh) * | 2019-01-09 | 2021-08-24 | 凯杰科学有限责任公司 | 检测分析物的方法及其组合物 |
| EP3998349A3 (en) | 2019-03-01 | 2022-08-24 | Mercy Bioanalytics, Inc. | Compositions and methods for target protein detection on exosomes using proximal ligation |
| WO2020220013A1 (en) * | 2019-04-26 | 2020-10-29 | Arizona Board Of Regents On Behalf Of Arizona State University | Proximity-driven activation of crispr-cas systems for detection of diverse molecular analytes |
| EP3963070A4 (en) | 2019-04-30 | 2023-02-22 | Encodia, Inc. | METHODS OF PREPARATION OF ANALYTES AND RELATED KITS |
| EP4069731A4 (en) | 2019-12-03 | 2024-05-29 | Alamar Biosciences, Inc. | Nucleic acid linked immune-sandwich assay (nulisa) |
| EP4070113A4 (en) | 2019-12-04 | 2023-12-20 | Biora Therapeutics, Inc. | ASSESSMENT OF PREECAMPSIA USING FREE AND DISSOCIATE PLACENTAL GROWTH FACTOR ASSAYS |
| GB202004469D0 (en) | 2020-03-27 | 2020-05-13 | Olink Proteomics Ab | Controls for proximity detection assays |
| CA3182266A1 (en) | 2020-06-11 | 2021-12-16 | Vadim Lobanov | Methods and systems for computational decoding of biological, chemical, and physical entities |
| EP4225946A4 (en) * | 2020-10-06 | 2024-10-30 | President And Fellows Of Harvard College | METHODS, COMPOSITIONS, KITS AND SYSTEMS FOR SIGNAL-BASED DETECTION OF NEUTRALIZATION TARGETS |
| GB202018503D0 (en) | 2020-11-25 | 2021-01-06 | Olink Proteomics Ab | Analyte detection method employing concatamers |
| KR102573998B1 (ko) * | 2020-12-30 | 2023-09-04 | 주식회사 스타노스 | 위양성 신호가 제거된 항원 검출방법 및 키트 |
| US12092642B2 (en) | 2021-01-20 | 2024-09-17 | Nautilus Subsidiary, Inc. | Systems and methods for biomolecule quantitation |
| AU2022209365A1 (en) | 2021-01-21 | 2023-07-20 | Nautilus Subsidiary, Inc. | Systems and methods for biomolecule preparation |
| IL305336B2 (en) | 2021-03-11 | 2025-09-01 | Nautilus Subsidiary Inc | Systems and methods for preserving biomolecules |
| US20230035685A1 (en) * | 2021-07-30 | 2023-02-02 | 10X Genomics, Inc. | One step in situ rolling circle amplification assay |
| CA3227872A1 (en) | 2021-09-09 | 2023-03-16 | James Henry JOLY | Characterization and localization of protein modifications |
| EP4405683A1 (en) | 2021-09-22 | 2024-07-31 | Nautilus Subsidiary, Inc. | Methods and systems for determining polypeptide interactions |
| WO2023081728A1 (en) | 2021-11-03 | 2023-05-11 | Nautilus Biotechnology, Inc. | Systems and methods for surface structuring |
| AU2023241893A1 (en) | 2022-03-29 | 2024-09-26 | Nautilus Subsidiary, Inc. | Integrated arrays for single-analyte processes |
| WO2023212490A1 (en) | 2022-04-25 | 2023-11-02 | Nautilus Subsidiary, Inc. | Systems and methods for assessing and improving the quality of multiplex molecular assays |
| US20250376716A1 (en) | 2022-06-21 | 2025-12-11 | Nautilus Subsidiary, Inc. | Method for detecting analytes at sites of optically non-resolvable distances |
| WO2024059655A1 (en) | 2022-09-15 | 2024-03-21 | Nautilus Subsidiary, Inc. | Characterizing accessibility of macromolecule structures |
| WO2024073599A1 (en) | 2022-09-29 | 2024-04-04 | Nautilus Subsidiary, Inc. | Preparation of array surfaces for single-analyte processes |
| EP4382534A1 (en) | 2022-12-06 | 2024-06-12 | Biomérieux | Synthetic peptide for use in an immunomolecular assay |
| WO2024124073A1 (en) | 2022-12-09 | 2024-06-13 | Nautilus Subsidiary, Inc. | A method comprising performing on a single-analyte array at least 50 cycles of a process |
| US20240201182A1 (en) | 2022-12-15 | 2024-06-20 | Nautilus Subsidiary, Inc. | Inhibition of photon phenomena on single molecule arrays |
| CN120858284A (zh) | 2023-01-27 | 2025-10-28 | 生物梅里埃公司 | 用于更快生物标志物检测的改良的免疫分子测定 |
| WO2024206122A1 (en) | 2023-03-24 | 2024-10-03 | Nautilus Subsidiary, Inc. | Improved transfer of nanoparticles to array surfaces |
| WO2024243237A1 (en) | 2023-05-24 | 2024-11-28 | Nautilus Subsidiary, Inc. | Systems and methods for machine vision |
| WO2025012398A1 (en) | 2023-07-11 | 2025-01-16 | Olink Proteomics Ab | Dried reagents |
| US20250066841A1 (en) | 2023-08-24 | 2025-02-27 | Nautilus Subsidiary, Inc. | Compositions and methods for detecting binding interactions under equilibrium or non-equilibrium conditions |
| WO2025111398A1 (en) | 2023-11-20 | 2025-05-30 | Alamar Biosciences, Inc. | Reduction of non-cognate signal in multiplex proximity ligation assays |
| WO2025111397A1 (en) | 2023-11-20 | 2025-05-30 | Alamar Biosciences, Inc. | Use of autoinhibition standard curves in proximity assays |
| WO2025111396A1 (en) | 2023-11-20 | 2025-05-30 | Alamar Biosciences, Inc. | Molecular barcode set and use thereof in multiplex proximity detection assays |
| CN117343999B (zh) * | 2023-12-05 | 2024-03-01 | 上海锐赛循益生物技术有限公司 | 基于左侧探针退火和右侧探针退火延伸的核酸扩增方法 |
| US20250189519A1 (en) | 2023-12-11 | 2025-06-12 | Nautilus Subsidiary, Inc. | Methods and compositions of particle-based arrays |
| WO2025137166A1 (en) | 2023-12-20 | 2025-06-26 | Nautilus Subsidiary, Inc. | Fluidic devices for single-analyte assays |
| WO2025168224A1 (en) | 2024-02-08 | 2025-08-14 | Leica Microsystems Cms Gmbh | Marker and method for analysing a biological sample |
| WO2025199005A1 (en) | 2024-03-20 | 2025-09-25 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Biomarkers for identifying cancer patients at high risk for chemotherapy-induced cardiotoxicity |
| WO2025199236A1 (en) | 2024-03-22 | 2025-09-25 | Nautilus Subsidiary, Inc. | Plasmonic particle systems for single-analyte assays |
| WO2025212338A1 (en) | 2024-04-01 | 2025-10-09 | Nautilus Subsidiary, Inc. | Nanostructures for modulation of analyte conformation |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6558928B1 (en) * | 1998-03-25 | 2003-05-06 | Ulf Landegren | Rolling circle replication of padlock probes |
| WO2006137932A2 (en) * | 2004-11-03 | 2006-12-28 | Leucadia Technologies, Inc. | Homogeneous analyte detection |
Family Cites Families (63)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU530410B2 (en) | 1978-02-21 | 1983-07-14 | Sintef | Preparing aqueous emulsions |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US4883867A (en) | 1985-11-01 | 1989-11-28 | Becton, Dickinson And Company | Detection of reticulocytes, RNA or DNA |
| US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
| US5266684A (en) | 1988-05-02 | 1993-11-30 | The Reagents Of The University Of California | Peptide mixtures |
| US5223409A (en) | 1988-09-02 | 1993-06-29 | Protein Engineering Corp. | Directed evolution of novel binding proteins |
| US5449603A (en) | 1989-10-24 | 1995-09-12 | Stratagene | Method for hybridizing nucleic acids using single-stranded nucleic acid binding protein |
| US5733523A (en) | 1990-12-10 | 1998-03-31 | Akzo Nobel N.V. | Targeted delivery of a therapeutic entity using complementary oligonucleotides |
| AU672367B2 (en) | 1991-09-13 | 1996-10-03 | British Biocell International Limited | Nucleic acids extension assay |
| ATE152831T1 (de) | 1991-09-16 | 1997-05-15 | Molecular Probes Inc | Dimere unsymmetrische cyaninfarbstoffe |
| CA2118806A1 (en) | 1991-09-18 | 1993-04-01 | William J. Dower | Method of synthesizing diverse collections of oligomers |
| US5639603A (en) | 1991-09-18 | 1997-06-17 | Affymax Technologies N.V. | Synthesizing and screening molecular diversity |
| AU3595493A (en) | 1992-01-28 | 1993-09-01 | President And Fellors Of Harvard College | Method for nucleic acid hybridization using single-stranded DNA binding protein |
| US5321130A (en) | 1992-02-10 | 1994-06-14 | Molecular Probes, Inc. | Unsymmetrical cyanine dyes with a cationic side chain |
| US5541061A (en) | 1992-04-29 | 1996-07-30 | Affymax Technologies N.V. | Methods for screening factorial chemical libraries |
| EP0651805B1 (en) | 1992-07-17 | 2006-12-13 | Dana Farber Cancer Institute | Method of intracellular binding of target molecules |
| US5288514A (en) | 1992-09-14 | 1994-02-22 | The Regents Of The University Of California | Solid phase and combinatorial synthesis of benzodiazepine compounds on a solid support |
| US5565324A (en) | 1992-10-01 | 1996-10-15 | The Trustees Of Columbia University In The City Of New York | Complex combinatorial chemical libraries encoded with tags |
| US5721099A (en) | 1992-10-01 | 1998-02-24 | Trustees Of Columbia University In The City Of New York | Complex combinatorial chemical libraries encoded with tags |
| US5271099A (en) | 1992-11-02 | 1993-12-21 | Lin Ming Huei | Sunshade cap with a double layer visor |
| US5410030A (en) | 1993-04-05 | 1995-04-25 | Molecular Probes, Inc. | Dimers of unsymmetrical cyanine dyes containing pyridinium moieties |
| US5436134A (en) | 1993-04-13 | 1995-07-25 | Molecular Probes, Inc. | Cyclic-substituted unsymmetrical cyanine dyes |
| US5658751A (en) | 1993-04-13 | 1997-08-19 | Molecular Probes, Inc. | Substituted unsymmetrical cyanine dyes with selected permeability |
| US5440016A (en) | 1993-06-18 | 1995-08-08 | Torrey Pines Institute For Molecular Studies | Peptides of the formula (KFmoc) ZZZ and their uses |
| US5851829A (en) | 1993-07-16 | 1998-12-22 | Dana-Farber Cancer Institute | Method of intracellular binding of target molecules |
| US5635602A (en) | 1993-08-13 | 1997-06-03 | The Regents Of The University Of California | Design and synthesis of bispecific DNA-antibody conjugates |
| US5593853A (en) | 1994-02-09 | 1997-01-14 | Martek Corporation | Generation and screening of synthetic drug libraries |
| US5512462A (en) | 1994-02-25 | 1996-04-30 | Hoffmann-La Roche Inc. | Methods and reagents for the polymerase chain reaction amplification of long DNA sequences |
| US5688997A (en) | 1994-05-06 | 1997-11-18 | Pharmacopeia, Inc. | Process for preparing intermediates for a combinatorial dihydrobenzopyran library |
| US5525735A (en) | 1994-06-22 | 1996-06-11 | Affymax Technologies Nv | Methods for synthesizing diverse collections of pyrrolidine compounds |
| US5549974A (en) | 1994-06-23 | 1996-08-27 | Affymax Technologies Nv | Methods for the solid phase synthesis of thiazolidinones, metathiazanones, and derivatives thereof |
| US5463564A (en) | 1994-09-16 | 1995-10-31 | 3-Dimensional Pharmaceuticals, Inc. | System and method of automatically generating chemical compounds with desired properties |
| US5688696A (en) | 1994-12-12 | 1997-11-18 | Selectide Corporation | Combinatorial libraries having a predetermined frequency of each species of test compound |
| SE504798C2 (sv) | 1995-06-16 | 1997-04-28 | Ulf Landegren | Immunanalys och testkit med två reagens som kan tvärbindas om de adsorberats till analyten |
| EP0839152B1 (en) | 1995-06-21 | 2002-03-13 | Martek Biosciences Corporation | Combinatorial libraries of labeled biochemical compounds and methods for producing same |
| US5854033A (en) | 1995-11-21 | 1998-12-29 | Yale University | Rolling circle replication reporter systems |
| US5731423A (en) | 1996-03-21 | 1998-03-24 | Transcell Technologies, Inc. | Process for preparing sulfoxides |
| US6117635A (en) | 1996-07-16 | 2000-09-12 | Intergen Company | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
| GB9703369D0 (en) | 1997-02-18 | 1997-04-09 | Lindqvist Bjorn H | Process |
| ES2209145T5 (es) | 1997-05-28 | 2009-10-30 | Crescendo Biologics Ltd. | Complejos de ribosomas como particulas de seleccion para el desarrollo in vitro y evolucion de proteinas. |
| ES2290979T3 (es) | 1997-12-15 | 2008-02-16 | Csl Behring Gmbh | Cebador marcado para uso en la deteccion de acidos nucleicos diana. |
| US5989823A (en) | 1998-09-18 | 1999-11-23 | Nexstar Pharmaceuticals, Inc. | Homogeneous detection of a target through nucleic acid ligand-ligand beacon interaction |
| GB9812768D0 (en) | 1998-06-13 | 1998-08-12 | Zeneca Ltd | Methods |
| SE516272C2 (sv) | 2000-02-18 | 2001-12-10 | Ulf Landegren | Metoder och kit för analytdetektion mha proximitets-probning |
| US6511809B2 (en) | 2000-06-13 | 2003-01-28 | E. I. Du Pont De Nemours And Company | Method for the detection of an analyte by means of a nucleic acid reporter |
| EP1451356A1 (en) | 2001-11-23 | 2004-09-01 | Simon Fredriksson | Method and kit for proximity probing with multivalent proximity probes |
| US7553619B2 (en) | 2002-02-08 | 2009-06-30 | Qiagen Gmbh | Detection method using dissociated rolling circle amplification |
| US20050221349A1 (en) | 2002-05-30 | 2005-10-06 | Stuart Wilson | Methods of detecting target molecules and molecular interactions |
| CN101410530B (zh) | 2003-04-18 | 2013-03-27 | 贝克顿·迪金森公司 | 免疫-扩增 |
| US7914987B2 (en) | 2004-06-14 | 2011-03-29 | The Board Of Trustees Of The Leland Stanford Junior University | Methods and compositions for use in analyte detection using proximity probes |
| WO2007005649A2 (en) | 2005-06-30 | 2007-01-11 | Applera Corporation | Proximity probing of target proteins comprising restriction and/or extension field |
| US20080293051A1 (en) | 2005-08-30 | 2008-11-27 | Board Of Regents, The University Of Texas System | proximity ligation assay |
| GB0605584D0 (en) | 2006-03-20 | 2006-04-26 | Olink Ab | Method for analyte detection using proximity probes |
| US20080090238A1 (en) | 2006-10-12 | 2008-04-17 | Dan-Hui Dorothy Yang | Increased sensitivity of proximity ligation assays |
| JP2010533867A (ja) | 2007-07-17 | 2010-10-28 | アジレント テクノロジーズ インコーポレイテッド | 分析物検出アッセイ |
| US7659069B2 (en) | 2007-08-31 | 2010-02-09 | Agilent Technologies, Inc. | Binary signaling assay using a split-polymerase |
| JP2010539942A (ja) | 2007-09-28 | 2010-12-24 | アフォミックス コーポレイション | タンパク質を複合体化するためのポリヌクレオチド骨格 |
| WO2009123216A1 (ja) * | 2008-03-31 | 2009-10-08 | 独立行政法人理化学研究所 | 高選択・高効率でｐｃｒ増幅が可能な新規ｄｎａ |
| US9040287B2 (en) * | 2010-02-12 | 2015-05-26 | Mediomics, Llc | Molecular biosensors capable of signal amplification |
| GB201011971D0 (en) | 2010-07-15 | 2010-09-01 | Olink Ab | Methods and product |
| GB201101621D0 (en) * | 2011-01-31 | 2011-03-16 | Olink Ab | Method and product |
-
2011
- 2011-01-31 GB GBGB1101621.9A patent/GB201101621D0/en not_active Ceased
-
2012
- 2012-01-30 EP EP12702018.8A patent/EP2670860B1/en active Active
- 2012-01-30 US US13/981,943 patent/US9777315B2/en active Active
- 2012-01-30 WO PCT/EP2012/051474 patent/WO2012104261A1/en not_active Ceased
- 2012-01-30 CN CN201280006856.9A patent/CN103354840B/zh active Active
- 2012-01-30 EP EP17183834.5A patent/EP3323896B1/en active Active
- 2012-01-30 JP JP2013550902A patent/JP6180940B2/ja active Active
-
2017
- 2017-02-06 JP JP2017019754A patent/JP6457564B2/ja active Active
- 2017-09-01 US US15/693,582 patent/US10731206B2/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6558928B1 (en) * | 1998-03-25 | 2003-05-06 | Ulf Landegren | Rolling circle replication of padlock probes |
| WO2006137932A2 (en) * | 2004-11-03 | 2006-12-28 | Leucadia Technologies, Inc. | Homogeneous analyte detection |
Non-Patent Citations (3)
| Title |
|---|
| Characterizing proteins and their interactions in cells and tissues using the in situ proximity ligation assay;Ola Söderberg et al;《Methods》;20080701;第45卷(第3期);227–232 * |
| Proximity ligation assays:a recent addition to the proteomics toolbox;Irene Weibrecht et al;《Expert Rev. Proteomics)》;20100601;第7卷(第3期);401–409 * |
| 蛋白质检测新技术-邻位连接技术及初步应用;唐娜等;《中国生物工程杂志》;20090831;第29卷(第8期);第113-118页，参见第1.1节，第2.3节，第2.4节 * |
Also Published As
| Publication number | Publication date |
|---|---|
| JP6180940B2 (ja) | 2017-08-16 |
| US20180100189A1 (en) | 2018-04-12 |
| US9777315B2 (en) | 2017-10-03 |
| JP6457564B2 (ja) | 2019-01-23 |
| EP3323896A2 (en) | 2018-05-23 |
| EP3323896A3 (en) | 2018-06-13 |
| CN103354840A (zh) | 2013-10-16 |
| EP2670860B1 (en) | 2017-08-16 |
| WO2012104261A1 (en) | 2012-08-09 |
| US10731206B2 (en) | 2020-08-04 |
| JP2017108749A (ja) | 2017-06-22 |
| EP3323896B1 (en) | 2020-05-27 |
| GB201101621D0 (en) | 2011-03-16 |
| EP2670860A1 (en) | 2013-12-11 |
| US20140030721A1 (en) | 2014-01-30 |
| JP2014504880A (ja) | 2014-02-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103354840B (zh) | 核酸外切酶可实现的邻近延伸分析 | |
| CN104114718B (zh) | 超嗜热聚合酶可实现的邻近延伸分析 | |
| US8268554B2 (en) | Method for analyte detection using proximity probes | |
| CN103154266B (zh) | 阻断试剂及其使用方法 | |
| US11034995B2 (en) | Proximity assay with detection based on hybridisation chain reaction (HCR) | |
| US7914987B2 (en) | Methods and compositions for use in analyte detection using proximity probes | |
| US10669569B2 (en) | Dynamic range methods | |
| CN103703145A (zh) | 解折叠邻近探针及其使用方法 | |
| CN116829735A (zh) | 检测靶核酸序列的方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| C56 | Change in the name or address of the patentee | ||
| CP01 | Change in the name or title of a patent holder |
Address after: uppsala Patentee after: European Ling Ke biological sciences Co. Address before: uppsala Patentee before: Olink AB |
|
| TR01 | Transfer of patent right |
Effective date of registration: 20170111 Address after: uppsala Patentee after: Proteomics, Inc. Address before: uppsala Patentee before: European Ling Ke biological sciences Co. |