CN103069009B - 环介导等温扩增(lamp)的序列特异性实时监测 - Google Patents

环介导等温扩增(lamp)的序列特异性实时监测 Download PDF

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CN103069009B
CN103069009B CN201180040896.0A CN201180040896A CN103069009B CN 103069009 B CN103069009 B CN 103069009B CN 201180040896 A CN201180040896 A CN 201180040896A CN 103069009 B CN103069009 B CN 103069009B
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probe
lamp
oligonucleotide chain
reaction
reaction mixture
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CN103069009A (zh
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久保田亮
丹尼尔·M·詹金斯
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University of Hawaii at Hilo
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
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  • Biomedical Technology (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201180040896.0A 2010-06-22 2011-06-22 环介导等温扩增(lamp)的序列特异性实时监测 Expired - Fee Related CN103069009B (zh)

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US35742810P 2010-06-22 2010-06-22
US61/357,428 2010-06-22
PCT/US2011/041540 WO2011163425A1 (en) 2010-06-22 2011-06-22 Sequence specific real-time monitoring of loop-mediated isothermal amplification (lamp)

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CN103069009A CN103069009A (zh) 2013-04-24
CN103069009B true CN103069009B (zh) 2015-01-14

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US (1) US20130171643A1 (enExample)
EP (1) EP2585617B1 (enExample)
JP (1) JP5984802B2 (enExample)
KR (1) KR101832011B1 (enExample)
CN (1) CN103069009B (enExample)
AU (1) AU2011270885B2 (enExample)
BR (1) BR112012032519A2 (enExample)
CA (1) CA2803161C (enExample)
MX (1) MX2012014917A (enExample)
WO (1) WO2011163425A1 (enExample)

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US9074249B2 (en) 2012-06-04 2015-07-07 New England Biolabs, Inc. Detection of amplification products
US9074243B2 (en) 2012-07-27 2015-07-07 New England Biolabs, Inc. Detection of amplification products
CN103540660B (zh) * 2013-10-10 2015-10-28 中国人民解放军疾病预防控制所 基于TaqMan探针的环介导恒温扩增法及其专用LAMP引物与试剂盒
CN103602727B (zh) * 2013-10-25 2015-03-11 中国林业科学研究院热带林业研究所 一种快速检测青枯菌的检测引物组、检测试剂盒和检测方法
US10106837B1 (en) 2014-02-05 2018-10-23 Steven A Benner Processes for point of care detection of DNA and RNA
KR101496835B1 (ko) 2014-03-04 2015-02-27 서울대학교산학협력단 살모넬라균 검출을 위한 등온증폭반응용 프라이머 조성물 및 이를 이용한 살모넬라균의 검출 방법
US10913973B2 (en) 2014-09-17 2021-02-09 Board Of Regents, The University Texas System Methods and devices related to toehold-based strand displacement with loop-mediated isothermal amplification
US10724091B1 (en) 2015-02-10 2020-07-28 National Technology & Engineering Solutions Of Sandia, Llc Endpoint detection of amplified nucleic acids
KR101552159B1 (ko) 2015-03-10 2015-09-10 경기대학교 산학협력단 고리-매개 등온 증폭법을 이용한 진균성 질병 검출 방법
CN104745694A (zh) * 2015-03-17 2015-07-01 苏州华麦生物科技有限公司 一种转基因水稻科丰6的恒温探针法检测引物组、检测试剂盒及检测方法
CN104946760B (zh) * 2015-06-16 2017-12-08 河南省农业科学院 一种检测肿囊腐霉的方法及专用试剂盒
KR101875662B1 (ko) * 2015-12-01 2018-08-02 에스디 바이오센서 주식회사 등온 핵산 증폭을 위한 리포터 염료, 퀀처가 포함되는 등온 기반 이중 기능성 올리고뉴클레오타이드 및 이를 이용한 핵산 증폭과 측정 방법
ITUB20159332A1 (it) * 2015-12-22 2017-06-22 Diasorin S P A Procedimento di rivelazione in fluorescenza dell?amplificazione isotermica mediata da loop (LAMP) di un acido nucleico bersaglio, relativi oligonucleotidi e kit.
WO2018027236A1 (en) * 2016-08-05 2018-02-08 Arizona Board Of Regents On Behalf Of Arizona State University Methods for digital readout quantification of nucleic acids
EP3290527B1 (en) * 2016-08-29 2019-06-19 MetaSystems Indigo GmbH Method for detecting microorganisms in a sample
US20210395810A1 (en) * 2016-11-30 2021-12-23 Steven A Benner Processes to Detect Coronaviruses
US11366116B1 (en) 2017-04-12 2022-06-21 National Technology & Engineering Solutions Of Sandia, Llc Real time autonomous surveillance of pathogens
GB201713251D0 (en) * 2017-08-18 2017-10-04 Lgc Genomics Ltd Methods and kits for detection of nucleic acid molecules
SG10201910254YA (en) 2019-11-04 2021-06-29 Denka Company Ltd Nucleic Acid Detection Method Using Lamp And Probe Detection
KR102687405B1 (ko) * 2020-10-16 2024-07-23 경북대학교 산학협력단 돼지 써코바이러스 3형 검출용 조성물 및 이를 이용한 돼지 써코바이러스 3형 검출 방법
WO2023106866A1 (ko) 2021-12-10 2023-06-15 주식회사 씨젠 단일 가닥 상호작용적 이중 표지 시스템을 포함하는 태그된 루프프라이머 및 이를 이용한 핵산 등온 증폭 방법
CN114196769B (zh) * 2021-12-27 2024-03-08 中国科学院长春应用化学研究所 一种用于检测沙门氏菌的引物、试剂盒及其使用方法
WO2024005458A1 (ko) 2022-06-27 2024-01-04 주식회사 씨젠 베타-하이드록시산을 이용한 타겟 핵산의 증폭 방법
CN119351619A (zh) * 2023-08-18 2025-01-24 福建师范大学 一种实时双重环介导等温扩增法检测猴痘病毒的引物探针组合、试剂盒及方法
CN117867152B (zh) * 2024-03-08 2024-05-24 云南英茂现代农业有限公司 一种用于检测青枯菌的核酸引物组、包含其的产品及应用

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EP2585617B1 (en) 2016-05-11
CA2803161A1 (en) 2011-12-29
EP2585617A1 (en) 2013-05-01
MX2012014917A (es) 2013-03-18
KR20130044303A (ko) 2013-05-02
BR112012032519A2 (pt) 2017-07-18
CA2803161C (en) 2019-09-24
EP2585617A4 (en) 2013-11-27
AU2011270885A1 (en) 2013-01-10
JP2013530705A (ja) 2013-08-01
AU2011270885B2 (en) 2016-05-19
HK1184830A1 (zh) 2014-01-30
CN103069009A (zh) 2013-04-24
JP5984802B2 (ja) 2016-09-07
WO2011163425A1 (en) 2011-12-29
KR101832011B1 (ko) 2018-02-23
US20130171643A1 (en) 2013-07-04

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