BR112012032519A2 - monitoramento em tempo real de amplificação isotérmica por loop(lamp) específico para sequência. - Google Patents

monitoramento em tempo real de amplificação isotérmica por loop(lamp) específico para sequência.

Info

Publication number
BR112012032519A2
BR112012032519A2 BR112012032519A BR112012032519A BR112012032519A2 BR 112012032519 A2 BR112012032519 A2 BR 112012032519A2 BR 112012032519 A BR112012032519 A BR 112012032519A BR 112012032519 A BR112012032519 A BR 112012032519A BR 112012032519 A2 BR112012032519 A2 BR 112012032519A2
Authority
BR
Brazil
Prior art keywords
lamp
real
sequence
referred
monitoring
Prior art date
Application number
BR112012032519A
Other languages
English (en)
Inventor
m jenkins Daniel
Kubota Ryo
Original Assignee
Univ Hawaii
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Hawaii filed Critical Univ Hawaii
Publication of BR112012032519A2 publication Critical patent/BR112012032519A2/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

Abstract

monitoramento em tempo real de amplificação isotérmica por loop(lamp) específico para sequência. diagnósticos baseados em gene capazes de rapidamente discriminar cepas selecionadas de um agente patogénico escolhido de outras populações da mesma espécie são divulgados. o monitoramento em tempo real de lamp de dna pode ser conseguido através do uso de sondas de oligonucleotídeos, referidas como "sondas de assimilação". as sondas de assimilação compreendem diuas cadeias de oligonucleotídeos, uma das quais compreende um extintor (referido como a sonda de extinção) e outro que compreende um fluoróforo (referido como sonda fluorescente) . um sinal fluorescente resulta quando as duas fitas são deslocadas uma da outra durante a reação de lamp. através do monitoramento da fluorescência emitida, a amplificação específica de sequência pode ser detectada.
BR112012032519A 2010-06-22 2011-06-22 monitoramento em tempo real de amplificação isotérmica por loop(lamp) específico para sequência. BR112012032519A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US35742810P 2010-06-22 2010-06-22
PCT/US2011/041540 WO2011163425A1 (en) 2010-06-22 2011-06-22 Sequence specific real-time monitoring of loop-mediated isothermal amplification (lamp)

Publications (1)

Publication Number Publication Date
BR112012032519A2 true BR112012032519A2 (pt) 2017-07-18

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
BR112012032519A BR112012032519A2 (pt) 2010-06-22 2011-06-22 monitoramento em tempo real de amplificação isotérmica por loop(lamp) específico para sequência.

Country Status (11)

Country Link
US (1) US20130171643A1 (pt)
EP (1) EP2585617B1 (pt)
JP (1) JP5984802B2 (pt)
KR (1) KR101832011B1 (pt)
CN (1) CN103069009B (pt)
AU (1) AU2011270885B2 (pt)
BR (1) BR112012032519A2 (pt)
CA (1) CA2803161C (pt)
HK (1) HK1184830A1 (pt)
MX (1) MX2012014917A (pt)
WO (1) WO2011163425A1 (pt)

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CN103602727B (zh) * 2013-10-25 2015-03-11 中国林业科学研究院热带林业研究所 一种快速检测青枯菌的检测引物组、检测试剂盒和检测方法
US10106837B1 (en) 2014-02-05 2018-10-23 Steven A Benner Processes for point of care detection of DNA and RNA
KR101496835B1 (ko) 2014-03-04 2015-02-27 서울대학교산학협력단 살모넬라균 검출을 위한 등온증폭반응용 프라이머 조성물 및 이를 이용한 살모넬라균의 검출 방법
US10913973B2 (en) * 2014-09-17 2021-02-09 Board Of Regents, The University Texas System Methods and devices related to toehold-based strand displacement with loop-mediated isothermal amplification
US10724091B1 (en) 2015-02-10 2020-07-28 National Technology & Engineering Solutions Of Sandia, Llc Endpoint detection of amplified nucleic acids
KR101552159B1 (ko) 2015-03-10 2015-09-10 경기대학교 산학협력단 고리-매개 등온 증폭법을 이용한 진균성 질병 검출 방법
CN104745694A (zh) * 2015-03-17 2015-07-01 苏州华麦生物科技有限公司 一种转基因水稻科丰6的恒温探针法检测引物组、检测试剂盒及检测方法
CN104946760B (zh) * 2015-06-16 2017-12-08 河南省农业科学院 一种检测肿囊腐霉的方法及专用试剂盒
KR101875662B1 (ko) * 2015-12-01 2018-08-02 에스디 바이오센서 주식회사 등온 핵산 증폭을 위한 리포터 염료, 퀀처가 포함되는 등온 기반 이중 기능성 올리고뉴클레오타이드 및 이를 이용한 핵산 증폭과 측정 방법
ITUB20159332A1 (it) 2015-12-22 2017-06-22 Diasorin S P A Procedimento di rivelazione in fluorescenza dell?amplificazione isotermica mediata da loop (LAMP) di un acido nucleico bersaglio, relativi oligonucleotidi e kit.
US11345954B2 (en) 2016-08-05 2022-05-31 Arizona Board Of Regents On Behalf Of Arizona State University Methods for digital readout quantification of nucleic acids
EP3290527B1 (en) * 2016-08-29 2019-06-19 MetaSystems Indigo GmbH Method for detecting microorganisms in a sample
US20210395810A1 (en) * 2016-11-30 2021-12-23 Steven A Benner Processes to Detect Coronaviruses
US11366116B1 (en) 2017-04-12 2022-06-21 National Technology & Engineering Solutions Of Sandia, Llc Real time autonomous surveillance of pathogens
GB201713251D0 (en) * 2017-08-18 2017-10-04 Lgc Genomics Ltd Methods and kits for detection of nucleic acid molecules
SG10201910254YA (en) 2019-11-04 2021-06-29 Denka Company Ltd Nucleic Acid Detection Method Using Lamp And Probe Detection
CN114196769B (zh) * 2021-12-27 2024-03-08 中国科学院长春应用化学研究所 一种用于检测沙门氏菌的引物、试剂盒及其使用方法
CN117867152A (zh) * 2024-03-08 2024-04-12 云南英茂现代农业有限公司 一种用于检测青枯菌的核酸引物组、包含其的产品及应用

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Also Published As

Publication number Publication date
AU2011270885A1 (en) 2013-01-10
CN103069009A (zh) 2013-04-24
EP2585617B1 (en) 2016-05-11
US20130171643A1 (en) 2013-07-04
JP2013530705A (ja) 2013-08-01
HK1184830A1 (zh) 2014-01-30
KR20130044303A (ko) 2013-05-02
JP5984802B2 (ja) 2016-09-07
CN103069009B (zh) 2015-01-14
EP2585617A4 (en) 2013-11-27
CA2803161A1 (en) 2011-12-29
MX2012014917A (es) 2013-03-18
WO2011163425A1 (en) 2011-12-29
EP2585617A1 (en) 2013-05-01
AU2011270885B2 (en) 2016-05-19
KR101832011B1 (ko) 2018-02-23
CA2803161C (en) 2019-09-24

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B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements