KR101832011B1 - 루프-매개 등온 증폭(ｌａｍｐ)의 서열 특이적 실시간 모니터링 - Google Patents

루프-매개 등온 증폭(ｌａｍｐ)의 서열 특이적 실시간 모니터링 Download PDF

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KR101832011B1
KR101832011B1 KR1020137001509A KR20137001509A KR101832011B1 KR 101832011 B1 KR101832011 B1 KR 101832011B1 KR 1020137001509 A KR1020137001509 A KR 1020137001509A KR 20137001509 A KR20137001509 A KR 20137001509A KR 101832011 B1 KR101832011 B1 KR 101832011B1
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probe
lamp
oligonucleotide strand
reaction
dna
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KR20130044303A (ko
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료 구보타
다니엘 엠. 젠킨스
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유니버시티 오브 하와이
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

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KR1020137001509A 2010-06-22 2011-06-22 루프-매개 등온 증폭(ｌａｍｐ)의 서열 특이적 실시간 모니터링 Expired - Fee Related KR101832011B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US35742810P 2010-06-22 2010-06-22
US61/357,428 2010-06-22
PCT/US2011/041540 WO2011163425A1 (en) 2010-06-22 2011-06-22 Sequence specific real-time monitoring of loop-mediated isothermal amplification (lamp)

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KR20130044303A KR20130044303A (ko) 2013-05-02
KR101832011B1 true KR101832011B1 (ko) 2018-02-23

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US (1) US20130171643A1 (enExample)
EP (1) EP2585617B1 (enExample)
JP (1) JP5984802B2 (enExample)
KR (1) KR101832011B1 (enExample)
CN (1) CN103069009B (enExample)
AU (1) AU2011270885B2 (enExample)
BR (1) BR112012032519A2 (enExample)
CA (1) CA2803161C (enExample)
MX (1) MX2012014917A (enExample)
WO (1) WO2011163425A1 (enExample)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013065574A1 (ja) 2011-10-31 2013-05-10 栄研化学株式会社 標的核酸の検出法
US9074249B2 (en) 2012-06-04 2015-07-07 New England Biolabs, Inc. Detection of amplification products
US9074243B2 (en) 2012-07-27 2015-07-07 New England Biolabs, Inc. Detection of amplification products
CN103540660B (zh) * 2013-10-10 2015-10-28 中国人民解放军疾病预防控制所 基于TaqMan探针的环介导恒温扩增法及其专用LAMP引物与试剂盒
CN103602727B (zh) * 2013-10-25 2015-03-11 中国林业科学研究院热带林业研究所 一种快速检测青枯菌的检测引物组、检测试剂盒和检测方法
US10106837B1 (en) 2014-02-05 2018-10-23 Steven A Benner Processes for point of care detection of DNA and RNA
KR101496835B1 (ko) 2014-03-04 2015-02-27 서울대학교산학협력단 살모넬라균 검출을 위한 등온증폭반응용 프라이머 조성물 및 이를 이용한 살모넬라균의 검출 방법
US10913973B2 (en) * 2014-09-17 2021-02-09 Board Of Regents, The University Texas System Methods and devices related to toehold-based strand displacement with loop-mediated isothermal amplification
US10724091B1 (en) 2015-02-10 2020-07-28 National Technology & Engineering Solutions Of Sandia, Llc Endpoint detection of amplified nucleic acids
KR101552159B1 (ko) 2015-03-10 2015-09-10 경기대학교 산학협력단 고리-매개 등온 증폭법을 이용한 진균성 질병 검출 방법
CN104745694A (zh) * 2015-03-17 2015-07-01 苏州华麦生物科技有限公司 一种转基因水稻科丰6的恒温探针法检测引物组、检测试剂盒及检测方法
CN104946760B (zh) * 2015-06-16 2017-12-08 河南省农业科学院 一种检测肿囊腐霉的方法及专用试剂盒
KR101875662B1 (ko) * 2015-12-01 2018-08-02 에스디 바이오센서 주식회사 등온 핵산 증폭을 위한 리포터 염료, 퀀처가 포함되는 등온 기반 이중 기능성 올리고뉴클레오타이드 및 이를 이용한 핵산 증폭과 측정 방법
ITUB20159332A1 (it) 2015-12-22 2017-06-22 Diasorin S P A Procedimento di rivelazione in fluorescenza dell?amplificazione isotermica mediata da loop (LAMP) di un acido nucleico bersaglio, relativi oligonucleotidi e kit.
US11345954B2 (en) 2016-08-05 2022-05-31 Arizona Board Of Regents On Behalf Of Arizona State University Methods for digital readout quantification of nucleic acids
EP3290527B1 (en) * 2016-08-29 2019-06-19 MetaSystems Indigo GmbH Method for detecting microorganisms in a sample
US20210395810A1 (en) * 2016-11-30 2021-12-23 Steven A Benner Processes to Detect Coronaviruses
US11366116B1 (en) 2017-04-12 2022-06-21 National Technology & Engineering Solutions Of Sandia, Llc Real time autonomous surveillance of pathogens
GB201713251D0 (en) * 2017-08-18 2017-10-04 Lgc Genomics Ltd Methods and kits for detection of nucleic acid molecules
SG10201910254YA (en) * 2019-11-04 2021-06-29 Denka Company Ltd Nucleic Acid Detection Method Using Lamp And Probe Detection
KR102687405B1 (ko) * 2020-10-16 2024-07-23 경북대학교 산학협력단 돼지 써코바이러스 3형 검출용 조성물 및 이를 이용한 돼지 써코바이러스 3형 검출 방법
KR20240104144A (ko) 2021-12-10 2024-07-04 주식회사 씨젠 단일 가닥 상호작용적 이중 표지 시스템을 포함하는 태그된 루프프라이머 및 이를 이용한 핵산 등온 증폭 방법
CN114196769B (zh) * 2021-12-27 2024-03-08 中国科学院长春应用化学研究所 一种用于检测沙门氏菌的引物、试剂盒及其使用方法
EP4545648A1 (en) 2022-06-27 2025-04-30 Seegene, Inc. Method for amplifying target nucleic acid using beta-hydroxy acid
CN119351619A (zh) * 2023-08-18 2025-01-24 福建师范大学 一种实时双重环介导等温扩增法检测猴痘病毒的引物探针组合、试剂盒及方法
CN117867152B (zh) * 2024-03-08 2024-05-24 云南英茂现代农业有限公司 一种用于检测青枯菌的核酸引物组、包含其的产品及应用

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080220425A1 (en) * 1996-01-24 2008-09-11 Third Wave Technologies, Inc. Methods and Compositions for Detecting Target Sequences
US20080085515A1 (en) * 2000-03-24 2008-04-10 Eppendorf Array Technologies Sa (Eat) Identification of multiple biological (micro) organisms by detection of their nucleotide sequences on arrays
PT1633888E (pt) * 2003-06-17 2009-04-22 Keygene Nv Meios e método para a detecção de sequências de nucleótidos alvo utilizando ensaios de ligação com pares de sondas de oligonucleótido melhoradas
FI20031678A0 (fi) * 2003-11-18 2003-11-18 Teemu Korpimaeki Näytteen käsittelymenetelmä ja järjestely siihen
US20060115838A1 (en) * 2004-10-19 2006-06-01 Trevigen Inc. Real-time detection of amplicons using nucleic acid repair enzymes
NZ548731A (en) * 2006-07-24 2008-12-24 Zygem Corp Ltd Isothermal detection methods and uses thereof
JP2009159945A (ja) * 2007-12-13 2009-07-23 Hitachi High-Technologies Corp 核酸検出用プローブ
WO2009108693A2 (en) * 2008-02-25 2009-09-03 The Government Of The United States Of America , As Represented By The Secretary, Department Of Health And Human Services, Composition and methods for rapid detection of hiv by loop- mediated isothermal amplification (lamp)

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Anal. Chem. 2002, 74, 3525-3532
J Virol Methods. 2008 Jul;151(1):55-60
Phytopathology. 2008 Sep;98(9):1045-51

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JP5984802B2 (ja) 2016-09-07
CA2803161C (en) 2019-09-24
EP2585617B1 (en) 2016-05-11
CA2803161A1 (en) 2011-12-29
MX2012014917A (es) 2013-03-18
CN103069009B (zh) 2015-01-14
WO2011163425A1 (en) 2011-12-29
BR112012032519A2 (pt) 2017-07-18
US20130171643A1 (en) 2013-07-04
KR20130044303A (ko) 2013-05-02
EP2585617A4 (en) 2013-11-27
AU2011270885A1 (en) 2013-01-10
HK1184830A1 (zh) 2014-01-30
JP2013530705A (ja) 2013-08-01
AU2011270885B2 (en) 2016-05-19
EP2585617A1 (en) 2013-05-01
CN103069009A (zh) 2013-04-24

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