JP2013521776A5 - - Google Patents
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- JP2013521776A5 JP2013521776A5 JP2012557157A JP2012557157A JP2013521776A5 JP 2013521776 A5 JP2013521776 A5 JP 2013521776A5 JP 2012557157 A JP2012557157 A JP 2012557157A JP 2012557157 A JP2012557157 A JP 2012557157A JP 2013521776 A5 JP2013521776 A5 JP 2013521776A5
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- nucleic acid
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- 238000000034 method Methods 0.000 claims description 44
- 150000007523 nucleic acids Chemical group 0.000 claims description 27
- 239000011541 reaction mixture Substances 0.000 claims description 27
- 108020004707 nucleic acids Proteins 0.000 claims description 24
- 102000039446 nucleic acids Human genes 0.000 claims description 24
- 239000000523 sample Substances 0.000 claims description 19
- 230000000295 complement effect Effects 0.000 claims description 14
- 238000002844 melting Methods 0.000 claims description 11
- 230000003321 amplification Effects 0.000 claims description 10
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 10
- 238000004925 denaturation Methods 0.000 claims description 9
- 230000036425 denaturation Effects 0.000 claims description 9
- 230000008018 melting Effects 0.000 claims description 9
- 108020004414 DNA Proteins 0.000 claims description 6
- 238000001816 cooling Methods 0.000 claims description 6
- 238000006243 chemical reaction Methods 0.000 claims description 5
- 125000003729 nucleotide group Chemical group 0.000 claims description 5
- 238000003753 real-time PCR Methods 0.000 claims description 5
- 238000012163 sequencing technique Methods 0.000 claims description 5
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 4
- 108091027305 Heteroduplex Proteins 0.000 claims description 4
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 4
- 239000002773 nucleotide Substances 0.000 claims description 4
- 238000012165 high-throughput sequencing Methods 0.000 claims description 3
- 108010006785 Taq Polymerase Proteins 0.000 claims description 2
- 230000015572 biosynthetic process Effects 0.000 claims description 2
- 239000012141 concentrate Substances 0.000 claims description 2
- 238000001514 detection method Methods 0.000 claims description 2
- 238000007847 digital PCR Methods 0.000 claims description 2
- 238000001840 matrix-assisted laser desorption--ionisation time-of-flight mass spectrometry Methods 0.000 claims description 2
- 238000007894 restriction fragment length polymorphism technique Methods 0.000 claims description 2
- 102000053602 DNA Human genes 0.000 claims 2
- 108091093037 Peptide nucleic acid Proteins 0.000 claims 2
- 108020004682 Single-Stranded DNA Proteins 0.000 claims 2
- 230000035772 mutation Effects 0.000 claims 2
- FGUUSXIOTUKUDN-IBGZPJMESA-N C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 Chemical compound C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 FGUUSXIOTUKUDN-IBGZPJMESA-N 0.000 claims 1
- 101000829171 Hypocrea virens (strain Gv29-8 / FGSC 10586) Effector TSP1 Proteins 0.000 claims 1
- DWAQJAXMDSEUJJ-UHFFFAOYSA-M Sodium bisulfite Chemical compound [Na+].OS([O-])=O DWAQJAXMDSEUJJ-UHFFFAOYSA-M 0.000 claims 1
- 238000012986 modification Methods 0.000 claims 1
- 230000004048 modification Effects 0.000 claims 1
- 108090000765 processed proteins & peptides Proteins 0.000 claims 1
- 238000012175 pyrosequencing Methods 0.000 claims 1
- 235000010267 sodium hydrogen sulphite Nutrition 0.000 claims 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 238000009396 hybridization Methods 0.000 description 4
- 238000000605 extraction Methods 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- 108700028369 Alleles Proteins 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 239000001046 green dye Substances 0.000 description 2
- PHTQWCKDNZKARW-UHFFFAOYSA-N isoamylol Chemical compound CC(C)CCO PHTQWCKDNZKARW-UHFFFAOYSA-N 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 description 1
- 239000005695 Ammonium acetate Substances 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 108010067770 Endopeptidase K Proteins 0.000 description 1
- 210000000712 G cell Anatomy 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 102000006382 Ribonucleases Human genes 0.000 description 1
- 108010083644 Ribonucleases Proteins 0.000 description 1
- 239000007984 Tris EDTA buffer Substances 0.000 description 1
- 229940043376 ammonium acetate Drugs 0.000 description 1
- 235000019257 ammonium acetate Nutrition 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 238000012869 ethanol precipitation Methods 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 238000012606 in vitro cell culture Methods 0.000 description 1
- 210000004880 lymph fluid Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 108091033319 polynucleotide Proteins 0.000 description 1
- 102000040430 polynucleotide Human genes 0.000 description 1
- 239000002157 polynucleotide Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000000700 radioactive tracer Substances 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 238000011897 real-time detection Methods 0.000 description 1
- 210000002345 respiratory system Anatomy 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 239000013535 sea water Substances 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 238000010583 slow cooling Methods 0.000 description 1
- 239000011343 solid material Substances 0.000 description 1
- 210000001179 synovial fluid Anatomy 0.000 description 1
- 210000001138 tear Anatomy 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US31164210P | 2010-03-08 | 2010-03-08 | |
| US61/311,642 | 2010-03-08 | ||
| PCT/US2011/027473 WO2011112534A1 (en) | 2010-03-08 | 2011-03-08 | Full cold-pcr enrichment with reference blocking sequence |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2013521776A JP2013521776A (ja) | 2013-06-13 |
| JP2013521776A5 true JP2013521776A5 (https=) | 2014-11-13 |
| JP5795341B2 JP5795341B2 (ja) | 2015-10-14 |
Family
ID=43983637
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012557157A Expired - Fee Related JP5795341B2 (ja) | 2010-03-08 | 2011-03-08 | 参照ブロック配列によるｆｕｌｌＣＯＬＤ−ＰＣＲ濃縮 |
Country Status (10)
| Country | Link |
|---|---|
| US (3) | US8623603B2 (https=) |
| EP (1) | EP2545189B1 (https=) |
| JP (1) | JP5795341B2 (https=) |
| KR (2) | KR101550489B1 (https=) |
| CN (1) | CN102859003B (https=) |
| AU (1) | AU2011224534B2 (https=) |
| CA (1) | CA2792433C (https=) |
| DK (1) | DK2545189T3 (https=) |
| ES (1) | ES2665500T3 (https=) |
| WO (1) | WO2011112534A1 (https=) |
Families Citing this family (47)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102859003B (zh) | 2010-03-08 | 2014-07-30 | 达纳-法伯癌症研究院有限公司 | 使用参考封闭序列的全cold-pcr富集 |
| AU2011319755B2 (en) | 2010-10-27 | 2017-02-23 | President And Fellows Of Harvard College | Compositions of toehold primer duplexes and methods of use |
| AU2012223438A1 (en) | 2011-02-28 | 2013-09-26 | Transgenomic, Inc | Kit and method for sequencing a target DNA in a mixed population |
| EP3333269B1 (en) * | 2011-03-31 | 2021-05-05 | Dana-Farber Cancer Institute, Inc. | Methods to enable multiplex cold-pcr |
| US9796748B2 (en) | 2011-05-02 | 2017-10-24 | President And Fellows Of Harvard College | Spatial sequestration of dynamic nucleic acid circuits |
| EP2758458A4 (en) | 2011-07-10 | 2015-10-21 | Harvard College | COMPOSITIONS AND METHODS FOR SELF-ASSEMBLING POLYMERS WITH COMPLEMENTARY MACROSCOPIC AND MICROSCOPIC SCALE UNITS |
| CN103131756B (zh) * | 2011-11-24 | 2014-08-27 | 百奥迈科生物技术有限公司 | 一种小核酸的检测方法及其应用 |
| JP5846496B2 (ja) * | 2012-04-19 | 2016-01-20 | 国立大学法人 鹿児島大学 | ＬＮＡクランプ技術による植物内生菌のＳＳＵｒＲＮＡ遺伝子の選択的ＰＣＲ増幅法 |
| US9133490B2 (en) | 2012-05-16 | 2015-09-15 | Transgenomic, Inc. | Step-up method for COLD-PCR enrichment |
| US9279146B2 (en) | 2012-12-21 | 2016-03-08 | Roche Molecular Systems, Inc. | Compounds and methods for the enrichment of mutated nucleic acid from a mixture |
| ES2730675T3 (es) | 2013-01-23 | 2019-11-12 | Univ Brandeis | Reactivos para mejorar la precisión de la PCR |
| US10913977B2 (en) | 2013-07-24 | 2021-02-09 | Dana-Farber Cancer Institute, Inc. | Methods and compositions to enable enrichment of minor DNA alleles by limiting denaturation time in PCR or simply enable enrichment of minor DNA alleles by limiting the denaturation time in PCR |
| US9624280B2 (en) | 2013-10-03 | 2017-04-18 | Janssen Biotech, Inc. | Protoxin-II variants and methods of use |
| AU2014336987A1 (en) * | 2013-10-19 | 2016-04-14 | Trovagene, Inc. | Detecting mutations in disease over time |
| US20160115556A1 (en) * | 2013-10-19 | 2016-04-28 | Trovagene, Inc. | Detecting mutations in disease over time |
| CN105283555A (zh) | 2013-10-20 | 2016-01-27 | 特罗瓦基因公司 | 核酸序列的合成和富集 |
| CA2922261A1 (en) * | 2013-10-20 | 2015-05-21 | Trovagene, Inc. | Synthesis and enrichment of nucleic acid sequences |
| US9873908B2 (en) | 2013-11-27 | 2018-01-23 | Roche Molecular Systems, Inc. | Methods for the enrichment of mutated nucleic acid from a mixture |
| CN103602748A (zh) * | 2013-11-28 | 2014-02-26 | 瑞希基因科技（北京）股份有限公司 | 一种结合荧光定量pcr技术的焦磷酸测序方法 |
| JP7016215B2 (ja) * | 2014-01-27 | 2022-02-04 | ザ ジェネラル ホスピタル コーポレイション | 配列決定のために核酸を調製する方法 |
| WO2015196154A1 (en) * | 2014-06-20 | 2015-12-23 | Richardson Katherine | System, method, data module and kit for detecting variant nucleotide sequences |
| JP6985151B2 (ja) * | 2015-04-02 | 2021-12-22 | ヤンセン バイオテツク，インコーポレーテツド | プロトキシン−ｉｉ変異体及びその使用方法 |
| EP3286334A4 (en) | 2015-04-20 | 2018-09-12 | Neogenomics Laboratories, Inc. | Method to increase sensitivity of next generation sequencing |
| CA2990846C (en) | 2015-06-24 | 2023-09-26 | Dana-Farber Cancer Institute, Inc. | Selective degradation of wild-type dna and enrichment of mutant alleles using nuclease |
| CN105063208B (zh) * | 2015-08-10 | 2018-03-06 | 北京吉因加科技有限公司 | 一种血浆中游离的目标dna低频突变富集测序方法 |
| WO2017070339A1 (en) * | 2015-10-20 | 2017-04-27 | Richardson Katherine | Microfluidic device for enrichment of nucleic acid sequence alterations |
| CN107338240B (zh) * | 2015-11-25 | 2020-11-24 | 葛猛 | 对样品中目标核酸序列进行偏向扩增的方法和试剂盒 |
| CN105821120B (zh) * | 2016-02-03 | 2019-04-30 | 广州医科大学附属肿瘤医院 | 一种基于巢式复合cold-pcr的t790m突变检测方法 |
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| FI3842545T3 (fi) | 2017-04-23 | 2023-01-31 | Koostumuksia ja menetelmiä näytteiden tunnistuksen parantamiseksi indeksoiduissa nukleiinihappokirjastoissa | |
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| JP7384912B2 (ja) | 2019-02-02 | 2023-11-21 | 北京字節跳動網絡技術有限公司 | 映像符号化におけるイントラブロックコピーのためのバッファにおけるデータ記憶 |
| CN120881292A (zh) | 2019-02-02 | 2025-10-31 | 北京字节跳动网络技术有限公司 | 使用用于视频编解码中的帧内块复制的额外缓冲区样点的预测 |
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| CN113545068B (zh) | 2019-03-01 | 2023-09-15 | 北京字节跳动网络技术有限公司 | 用于视频编解码中的帧内块复制的基于顺序的更新 |
| KR20210125506A (ko) | 2019-03-04 | 2021-10-18 | 베이징 바이트댄스 네트워크 테크놀로지 컴퍼니, 리미티드 | 비디오 코딩에서 인트라 블록 복사를 위한 버퍼 관리 |
| KR102677020B1 (ko) | 2019-07-06 | 2024-06-19 | 베이징 바이트댄스 네트워크 테크놀로지 컴퍼니, 리미티드 | 비디오 코딩에서 인트라 블록 카피를 위한 가상 예측 버퍼 |
| CN114175633B (zh) | 2019-07-10 | 2023-12-29 | 北京字节跳动网络技术有限公司 | 用于视频编解码中的帧内块复制的样点标识 |
| CN114097221B (zh) | 2019-07-11 | 2024-01-12 | 北京字节跳动网络技术有限公司 | 用于视频编解码中的帧内块复制的比特流一致性约束 |
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- 2011-03-08 WO PCT/US2011/027473 patent/WO2011112534A1/en not_active Ceased
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- 2011-03-08 KR KR1020157014456A patent/KR20150067398A/ko not_active Withdrawn
- 2011-03-08 EP EP11709836.8A patent/EP2545189B1/en not_active Not-in-force
- 2011-03-08 US US13/042,549 patent/US8623603B2/en not_active Expired - Fee Related
- 2011-03-08 ES ES11709836.8T patent/ES2665500T3/es active Active
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