CN102859003B - 使用参考封闭序列的全cold-pcr富集 - Google Patents

使用参考封闭序列的全cold-pcr富集 Download PDF

Info

Publication number
CN102859003B
CN102859003B CN201180020433.8A CN201180020433A CN102859003B CN 102859003 B CN102859003 B CN 102859003B CN 201180020433 A CN201180020433 A CN 201180020433A CN 102859003 B CN102859003 B CN 102859003B
Authority
CN
China
Prior art keywords
sequence
target
pcr
temperature
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201180020433.8A
Other languages
English (en)
Chinese (zh)
Other versions
CN102859003A (zh
Inventor
G·马克里吉奥古斯
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Dana Farber Cancer Institute Inc
Original Assignee
Dana Farber Cancer Institute Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dana Farber Cancer Institute Inc filed Critical Dana Farber Cancer Institute Inc
Publication of CN102859003A publication Critical patent/CN102859003A/zh
Application granted granted Critical
Publication of CN102859003B publication Critical patent/CN102859003B/zh
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN201180020433.8A 2010-03-08 2011-03-08 使用参考封闭序列的全cold-pcr富集 Expired - Fee Related CN102859003B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US31164210P 2010-03-08 2010-03-08
US61/311,642 2010-03-08
PCT/US2011/027473 WO2011112534A1 (en) 2010-03-08 2011-03-08 Full cold-pcr enrichment with reference blocking sequence

Publications (2)

Publication Number Publication Date
CN102859003A CN102859003A (zh) 2013-01-02
CN102859003B true CN102859003B (zh) 2014-07-30

Family

ID=43983637

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201180020433.8A Expired - Fee Related CN102859003B (zh) 2010-03-08 2011-03-08 使用参考封闭序列的全cold-pcr富集

Country Status (10)

Country Link
US (3) US8623603B2 (https=)
EP (1) EP2545189B1 (https=)
JP (1) JP5795341B2 (https=)
KR (2) KR20150067398A (https=)
CN (1) CN102859003B (https=)
AU (1) AU2011224534B2 (https=)
CA (1) CA2792433C (https=)
DK (1) DK2545189T3 (https=)
ES (1) ES2665500T3 (https=)
WO (1) WO2011112534A1 (https=)

Families Citing this family (47)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2011224534B2 (en) 2010-03-08 2014-10-09 Dana-Farber Cancer Institute, Inc. Full COLD-PCR enrichment with reference blocking sequence
EP2633071B1 (en) 2010-10-27 2016-10-12 President and Fellows of Harvard College Compositions of toehold primer duplexes and methods of use
JP2014507950A (ja) 2011-02-28 2014-04-03 トランスゲノミック，インク． 混合個体群における標的ｄｎａを配列決定するためのキットおよび方法
EP2691541B1 (en) * 2011-03-31 2017-10-18 Dana-Farber Cancer Institute, Inc. Method for enriching in single-stranded mutant sequences from mixture of wild-type and mutant sequences
US9796748B2 (en) 2011-05-02 2017-10-24 President And Fellows Of Harvard College Spatial sequestration of dynamic nucleic acid circuits
US9617392B2 (en) 2011-07-10 2017-04-11 President And Fellows Of Harvard College Compositions and methods for self-assembly of polymers with complementary macroscopic and microscopic scale units
CN103131756B (zh) * 2011-11-24 2014-08-27 百奥迈科生物技术有限公司 一种小核酸的检测方法及其应用
JP5846496B2 (ja) * 2012-04-19 2016-01-20 国立大学法人 鹿児島大学 ＬＮＡクランプ技術による植物内生菌のＳＳＵｒＲＮＡ遺伝子の選択的ＰＣＲ増幅法
US9133490B2 (en) 2012-05-16 2015-09-15 Transgenomic, Inc. Step-up method for COLD-PCR enrichment
US9279146B2 (en) 2012-12-21 2016-03-08 Roche Molecular Systems, Inc. Compounds and methods for the enrichment of mutated nucleic acid from a mixture
US10301688B2 (en) * 2013-01-23 2019-05-28 Brandeis University Reagents for improving PCR accuracy
US10913977B2 (en) * 2013-07-24 2021-02-09 Dana-Farber Cancer Institute, Inc. Methods and compositions to enable enrichment of minor DNA alleles by limiting denaturation time in PCR or simply enable enrichment of minor DNA alleles by limiting the denaturation time in PCR
MX2016004282A (es) 2013-10-03 2016-10-12 Janssen Biotech Inc Variantes de protoxina-ii y metodos de uso.
US20160115556A1 (en) * 2013-10-19 2016-04-28 Trovagene, Inc. Detecting mutations in disease over time
HK1222209A1 (zh) * 2013-10-19 2017-06-23 Trovagene, Inc. 隨時間檢測疾病中的突變
CA2922261A1 (en) * 2013-10-20 2015-05-21 Trovagene, Inc. Synthesis and enrichment of nucleic acid sequences
CN105283555A (zh) * 2013-10-20 2016-01-27 特罗瓦基因公司 核酸序列的合成和富集
US9873908B2 (en) 2013-11-27 2018-01-23 Roche Molecular Systems, Inc. Methods for the enrichment of mutated nucleic acid from a mixture
CN103602748A (zh) * 2013-11-28 2014-02-26 瑞希基因科技（北京）股份有限公司 一种结合荧光定量pcr技术的焦磷酸测序方法
EP3099820A4 (en) * 2014-01-27 2018-01-03 The General Hospital Corporation Methods of preparing nucleic acids for sequencing
WO2015196154A1 (en) * 2014-06-20 2015-12-23 Richardson Katherine System, method, data module and kit for detecting variant nucleotide sequences
EP3277304B1 (en) * 2015-04-02 2021-08-04 Janssen Biotech, Inc. Protoxin-ii variants and methods of use
CN107849606A (zh) * 2015-04-20 2018-03-27 尼欧基因组学实验室股份有限公司 提高下一代测序灵敏度的方法
CA2990846C (en) 2015-06-24 2023-09-26 Dana-Farber Cancer Institute, Inc. Selective degradation of wild-type dna and enrichment of mutant alleles using nuclease
CN105063208B (zh) * 2015-08-10 2018-03-06 北京吉因加科技有限公司 一种血浆中游离的目标dna低频突变富集测序方法
WO2017070339A1 (en) * 2015-10-20 2017-04-27 Richardson Katherine Microfluidic device for enrichment of nucleic acid sequence alterations
CN107338240B (zh) * 2015-11-25 2020-11-24 葛猛 对样品中目标核酸序列进行偏向扩增的方法和试剂盒
CN105821120B (zh) * 2016-02-03 2019-04-30 广州医科大学附属肿瘤医院 一种基于巢式复合cold-pcr的t790m突变检测方法
WO2018111835A1 (en) 2016-12-12 2018-06-21 Dana-Farber Cancer Institute, Inc. Compositions and methods for molecular barcoding of dna molecules prior to mutation enrichment and/or mutation detection
AU2018260627B2 (en) 2017-04-23 2024-08-22 Illumina Cambridge Limited Compositions and methods for improving sample identification in indexed nucleic acid libraries
AU2018259206B2 (en) * 2017-04-23 2024-07-11 Illumina Cambridge Limited Compositions and methods for improving sample identification in indexed nucleic acid libraries
CN110770353B (zh) 2017-04-23 2024-11-26 伊鲁米那股份有限公司 用于改进编索引的核酸文库中的样品鉴定的组合物和方法
US12584169B2 (en) 2017-04-23 2026-03-24 Illumina, Inc. Compositions and methods for improving sample identification in indexed nucleic acid libraries
EP3913053A1 (en) 2017-04-23 2021-11-24 Illumina Cambridge Limited Compositions and methods for improving sample identification in indexed nucleic acid libraries
US11174511B2 (en) 2017-07-24 2021-11-16 Dana-Farber Cancer Institute, Inc. Methods and compositions for selecting and amplifying DNA targets in a single reaction mixture
CN107858401A (zh) * 2017-10-31 2018-03-30 重庆邮电大学 一种低丰度基因突变的富集方法
WO2020156549A1 (en) 2019-02-02 2020-08-06 Beijing Bytedance Network Technology Co., Ltd. Buffer access methods for intra block copy in video coding
SG11202107959WA (en) 2019-02-02 2021-08-30 Beijing Bytedance Network Technology Co Ltd Buffer management for intra block copy in video coding
CN113545068B (zh) * 2019-03-01 2023-09-15 北京字节跳动网络技术有限公司 用于视频编解码中的帧内块复制的基于顺序的更新
EP3915265A4 (en) 2019-03-01 2022-06-22 Beijing Bytedance Network Technology Co., Ltd. DIRECTION-BASED PREDICTION FOR INTRA BLOCK COPY IN VIDEO CODING
KR20240132530A (ko) 2019-03-04 2024-09-03 베이징 바이트댄스 네트워크 테크놀로지 컴퍼니, 리미티드 비디오 코딩에서 인트라 블록 복사의 구현 형태
CN117294841A (zh) 2019-07-06 2023-12-26 北京字节跳动网络技术有限公司 用于视频编解码中的帧内块复制的虚拟预测缓冲
KR102635519B1 (ko) 2019-07-10 2024-02-07 베이징 바이트댄스 네트워크 테크놀로지 컴퍼니, 리미티드 비디오 코딩에서 인트라 블록 카피를 위한 샘플 식별
KR102695788B1 (ko) 2019-07-11 2024-08-14 베이징 바이트댄스 네트워크 테크놀로지 컴퍼니, 리미티드 비디오 코딩에서 인트라 블록 복사를 위한 비트스트림 적합 제약
WO2021034999A1 (en) 2019-08-21 2021-02-25 Dana-Farber Cancer Institute, Inc. Multi-site enrichment of deletions in dna microsatellites
JP2024531713A (ja) * 2021-09-13 2024-08-29 ネクスジェン キャンサー ディテクション エルエルシー 試料中の標的ポリヌクレオチドを検出するための方法
WO2025079958A1 (ko) * 2023-10-11 2025-04-17 고려대학교 산학협력단 높은 처리량의 돌연변이 특이적 아답터 리게이션 방법

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5849497A (en) * 1997-04-03 1998-12-15 The Research Foundation Of State University Of New York Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker
WO2009017784A2 (en) * 2007-08-01 2009-02-05 Dana-Farber Cancer Institute Enrichment of a target sequence
WO2009019008A1 (en) * 2007-08-08 2009-02-12 Roche Diagnostics Gmbh Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5'-3' exonuclease activity

Family Cites Families (73)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5310652A (en) 1986-08-22 1994-05-10 Hoffman-La Roche Inc. Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription
US5541308A (en) 1986-11-24 1996-07-30 Gen-Probe Incorporated Nucleic acid probes for detection and/or quantitation of non-viral organisms
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
GB2228086A (en) 1988-11-25 1990-08-15 Ici Plc Characterisation of genomic DNA
KR920700360A (ko) 1989-03-22 1992-02-19 하리크 프리드리히 미끄럼 베어링
US5075217A (en) 1989-04-21 1991-12-24 Marshfield Clinic Length polymorphisms in (dC-dA)n ·(dG-dT)n sequences
AU5645690A (en) 1989-05-05 1990-11-29 Lifecodes Corporation Method for genetic analysis of a nucleic acid sample
US5256775A (en) * 1989-06-05 1993-10-26 Gilead Sciences, Inc. Exonuclease-resistant oligonucleotides
CA2020958C (en) 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
US5045450A (en) 1989-07-13 1991-09-03 Massachusetts Institute Of Technology Determination of a mutational spectrum
DE3938907C2 (de) 1989-11-24 1999-11-04 Dade Behring Marburg Gmbh Mittel zum Lagern und Suspendieren von Zellen, insbesondere Erythrozyten
US5427930A (en) 1990-01-26 1995-06-27 Abbott Laboratories Amplification of target nucleic acids using gap filling ligase chain reaction
CA2035010C (en) 1990-01-26 1996-12-10 Keith C. Backman Method of amplifying target nucleic acids applicable to both polymerase and ligase chain reactions
EP0516753A4 (en) 1990-02-26 1994-05-18 Us Health A method for the fluorescent detection of a dna sequence in real time
WO1991014003A2 (en) 1990-03-09 1991-09-19 Ig Laboratories, Inc. Characterization and analysis of polymorphic vntr loci
ATE153707T1 (de) 1990-05-07 1997-06-15 Daikin Ind Ltd Diagnostische anwendungen der bildung von doppelten d-loops.
US5846710A (en) 1990-11-02 1998-12-08 St. Louis University Method for the detection of genetic diseases and gene sequence variations by single nucleotide primer extension
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
GB2293238A (en) 1994-09-13 1996-03-20 Inceltec Ltd Primers for replication and/or amplification reactions
US5629178A (en) * 1994-10-28 1997-05-13 Genetics & Ivf Institute Method for enhancing amplification in the polymerase chain reaction employing peptide nucleic acid (PNA)
US5565340A (en) 1995-01-27 1996-10-15 Clontech Laboratories, Inc. Method for suppressing DNA fragment amplification during PCR
US5631147A (en) 1995-09-21 1997-05-20 Becton, Dickinson And Company Detection of nucleic acids in cells by thermophilic strand displacement amplification
DK0862656T3 (da) 1995-11-21 2001-04-09 Univ Yale Unimolekylær segmentamplifikation og -detektering
US5612473A (en) 1996-01-16 1997-03-18 Gull Laboratories Methods, kits and solutions for preparing sample material for nucleic acid amplification
US6010878A (en) 1996-05-20 2000-01-04 Smithkline Beecham Corporation Interleukin-1 β converting enzyme like apoptotic protease-6
US6599704B2 (en) 1997-03-04 2003-07-29 Christoph Wagener Method for detecting mutated alleles
JP3785517B2 (ja) 1997-06-05 2006-06-14 東ソー株式会社 核酸融解温度測定法
JP4236812B2 (ja) 1997-09-12 2009-03-11 エクシコン エ／エス オリゴヌクレオチド類似体
US6174680B1 (en) 1998-12-30 2001-01-16 Dana-Farber Cancer Institute, Inc. Method for identifying mismatch repair glycosylase reactive sites, compounds and uses thereof
FR2779154B1 (fr) 1998-05-27 2002-07-12 Bio Merieux Procede d'amplification d'au moins une sequence nucleotidique particuliere et amorces de mise en oeuvre
US6994962B1 (en) 1998-12-09 2006-02-07 Massachusetts Institute Of Technology Methods of identifying point mutations in a genome
US6850846B2 (en) 2000-01-11 2005-02-01 Affymetrix, Inc. Computer software for genotyping analysis using pattern recognition
DE10012540B4 (de) 2000-03-15 2004-09-23 Vermicon Ag Oligonukleotide und Verfahren zum spezifischen Nachweis von Mikroorganismen durch Polymerase-Kettenreaktion
WO2002018659A2 (en) 2000-08-30 2002-03-07 Haplogen, Llc Method for determining alleles
EP1253205A1 (en) 2001-04-24 2002-10-30 LION Bioscience AG Method of blocking amplification of selected sequences
US6762049B2 (en) 2001-07-05 2004-07-13 Institute Of Microelectronics Miniaturized multi-chamber thermal cycler for independent thermal multiplexing
DE10158516A1 (de) 2001-11-29 2003-06-12 Focusgenomics Gmbh Verfahren zum Nachweis von Hybridisierungsereignissen in Nukleinsäuren
AUPS076802A0 (en) 2002-02-26 2002-03-21 Commonwealth Scientific And Industrial Research Organisation Selective nucleic acid amplification
AUPS076902A0 (en) 2002-02-26 2002-03-21 Commonwealth Scientific And Industrial Research Organisation Novel selective polymerase chain reaction
US7297484B2 (en) 2002-04-26 2007-11-20 Idaho Technology Characterization of single stranded nucleic acids by melting analysis of secondary structure using double strand-specific nucleic acid dye
US20040022764A1 (en) * 2002-07-31 2004-02-05 Hanan Polansky Inhibition of microcompetition with a foreign polynucleotide as treatment of chronic disease
AU2003289651A1 (en) 2002-12-18 2004-07-09 Dingbang Xu Pcr method and application by transnormal low thermo-denaturation temperature
US7306913B2 (en) * 2002-12-20 2007-12-11 Applera Corporation Genetic polymorphisms associated with coronary stenosis, methods of detection and uses thereof
GB0406863D0 (en) 2004-03-26 2004-04-28 Qiagen As Nucleic acid sequencing
JPWO2006093082A1 (ja) 2005-03-01 2008-08-07 和光純薬工業株式会社 サブトラクション・ポリヌクレオチドの取得方法
US7456281B2 (en) * 2005-04-20 2008-11-25 Idaho Technology, Inc. Nucleic acid melting analysis with saturation dyes
WO2007047572A2 (en) 2005-10-14 2007-04-26 Cleveland State University Methods for identifying multiple dna alteration markers in a large background of wild-type dna
US20070154892A1 (en) 2005-12-30 2007-07-05 Simon Wain-Hobson Differential amplification of mutant nucleic acids by PCR in a mixure of nucleic acids
WO2007087310A2 (en) 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Nucleic acid analysis using sequence tokens
WO2007106534A2 (en) * 2006-03-14 2007-09-20 Harbor-Ucla Research And Education Institute Selective amplification of minority mutations using primer blocking high-affinity oligonucleotides
JP5336350B2 (ja) 2006-03-28 2013-11-06 コモンウェルス サイエンティフィック アンドインダストリアル リサーチ オーガナイゼーション Ｄｎａフラグメントの増幅
US20080269068A1 (en) 2007-02-06 2008-10-30 President And Fellows Of Harvard College Multiplex decoding of sequence tags in barcodes
EP2171097A2 (en) 2007-06-29 2010-04-07 Population Genetics Technologies LTD. Methods and compositions for isolating nucleic acid sequence variants
WO2010065626A1 (en) 2008-12-02 2010-06-10 Globeimmune, Inc. Genotyping tools, methods and kits
US8206929B2 (en) * 2009-01-07 2012-06-26 Roche Molecular Systems, Inc. Nucleic acid amplification with allele-specific suppression of sequence variants
AU2010276236B2 (en) 2009-07-21 2014-03-20 Gen-Probe Incorporated Methods and compositions for quantitative detection of nucleic acid sequences over an extended dynamic range
AU2011224534B2 (en) 2010-03-08 2014-10-09 Dana-Farber Cancer Institute, Inc. Full COLD-PCR enrichment with reference blocking sequence
JP2014507950A (ja) 2011-02-28 2014-04-03 トランスゲノミック，インク． 混合個体群における標的ｄｎａを配列決定するためのキットおよび方法
WO2012129363A2 (en) 2011-03-24 2012-09-27 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
EP2691541B1 (en) 2011-03-31 2017-10-18 Dana-Farber Cancer Institute, Inc. Method for enriching in single-stranded mutant sequences from mixture of wild-type and mutant sequences
JP6040227B2 (ja) 2011-05-19 2016-12-07 アジーナ バイオサイエンス， インコーポレイテッド マルチプレックス核酸同定のための製品およびプロセス
US9238836B2 (en) 2012-03-30 2016-01-19 Pacific Biosciences Of California, Inc. Methods and compositions for sequencing modified nucleic acids
US9133490B2 (en) 2012-05-16 2015-09-15 Transgenomic, Inc. Step-up method for COLD-PCR enrichment
JP6009649B2 (ja) 2013-03-14 2016-10-19 タカラバイオ株式会社 耐熱性のミスマッチエンドヌクレアーゼの利用方法
CA2908361C (en) 2013-04-17 2023-09-26 Pioneer Hi-Bred International, Inc. Methods for characterizing dna sequence composition in a genome
US10913977B2 (en) 2013-07-24 2021-02-09 Dana-Farber Cancer Institute, Inc. Methods and compositions to enable enrichment of minor DNA alleles by limiting denaturation time in PCR or simply enable enrichment of minor DNA alleles by limiting the denaturation time in PCR
US9873908B2 (en) 2013-11-27 2018-01-23 Roche Molecular Systems, Inc. Methods for the enrichment of mutated nucleic acid from a mixture
WO2018111835A1 (en) 2016-12-12 2018-06-21 Dana-Farber Cancer Institute, Inc. Compositions and methods for molecular barcoding of dna molecules prior to mutation enrichment and/or mutation detection

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5849497A (en) * 1997-04-03 1998-12-15 The Research Foundation Of State University Of New York Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker
WO2009017784A2 (en) * 2007-08-01 2009-02-05 Dana-Farber Cancer Institute Enrichment of a target sequence
WO2009019008A1 (en) * 2007-08-08 2009-02-12 Roche Diagnostics Gmbh Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5'-3' exonuclease activity

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Coamplification at lower denaturation temperature-PCR increases mutation-detection selectivity of taqman-based real-time pcr;Li J et al.;《Clinical Chemistry》;20090929;第55卷(第4期);摘要，材料和方法，结论部分，图1-6 *
Wild-type blocking polymerase chain reaction for detection of single nucleotide minority mutations from clinical specimens;Dominguez PL et al.;《Oncogene》;20050822;第24卷(第45期);摘要，引言，结论部分，图1-4 *

Also Published As

Publication number Publication date
AU2011224534A1 (en) 2012-09-27
US11174510B2 (en) 2021-11-16
US20110217714A1 (en) 2011-09-08
CA2792433A1 (en) 2011-09-15
EP2545189B1 (en) 2018-01-10
WO2011112534A1 (en) 2011-09-15
US20140106362A1 (en) 2014-04-17
EP2545189A1 (en) 2013-01-16
DK2545189T3 (en) 2018-04-23
CA2792433C (en) 2016-10-11
US20180282798A1 (en) 2018-10-04
US9957556B2 (en) 2018-05-01
CN102859003A (zh) 2013-01-02
AU2011224534B2 (en) 2014-10-09
KR20120139780A (ko) 2012-12-27
KR20150067398A (ko) 2015-06-17
US8623603B2 (en) 2014-01-07
KR101550489B1 (ko) 2015-09-07
ES2665500T3 (es) 2018-04-26
JP2013521776A (ja) 2013-06-13
JP5795341B2 (ja) 2015-10-14

Similar Documents

Publication Publication Date Title
CN102859003B (zh) 使用参考封闭序列的全cold-pcr富集
CN105164280B (zh) 使用阻断性寡核苷酸进行dna扩增的方法
US9133490B2 (en) Step-up method for COLD-PCR enrichment
CN107849603B (zh) 利用有限核苷酸组成的引物扩增
AU2016281718B2 (en) Selective degradation of wild-type DNA and enrichment of mutant alleles using nuclease
US9518292B2 (en) Methods for suppression PCR
JP5805064B2 (ja) 対立遺伝子変種を検出するための方法、組成物、およびキット
US12098368B2 (en) Exponential base-3 and greater nucleic acid amplification with reduced amplification time
WO2017048863A1 (en) Step-up method for ice cold-pcr enrichment
US20250092448A1 (en) Methods and compositions for nucleic acid amplifications
US20160201105A1 (en) Step-up method for cold-pcr enrichment
HK40064470A (en) Amplification with primers of limited nucleotide composition

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20140730