DK2545189T3 - COMPLETE COLD-PCR ENRICHMENT WITH REFERENCE BLOCKING SEQUENCE - Google Patents
COMPLETE COLD-PCR ENRICHMENT WITH REFERENCE BLOCKING SEQUENCE Download PDFInfo
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- DK2545189T3 DK2545189T3 DK11709836.8T DK11709836T DK2545189T3 DK 2545189 T3 DK2545189 T3 DK 2545189T3 DK 11709836 T DK11709836 T DK 11709836T DK 2545189 T3 DK2545189 T3 DK 2545189T3
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- DK
- Denmark
- Prior art keywords
- sequence
- target
- pcr
- nucleic acid
- reaction mixture
- Prior art date
Links
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/107—Temperature of melting, i.e. Tm
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US31164210P | 2010-03-08 | 2010-03-08 | |
| PCT/US2011/027473 WO2011112534A1 (en) | 2010-03-08 | 2011-03-08 | Full cold-pcr enrichment with reference blocking sequence |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2545189T3 true DK2545189T3 (en) | 2018-04-23 |
Family
ID=43983637
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK11709836.8T DK2545189T3 (en) | 2010-03-08 | 2011-03-08 | COMPLETE COLD-PCR ENRICHMENT WITH REFERENCE BLOCKING SEQUENCE |
Country Status (10)
| Country | Link |
|---|---|
| US (3) | US8623603B2 (https=) |
| EP (1) | EP2545189B1 (https=) |
| JP (1) | JP5795341B2 (https=) |
| KR (2) | KR101550489B1 (https=) |
| CN (1) | CN102859003B (https=) |
| AU (1) | AU2011224534B2 (https=) |
| CA (1) | CA2792433C (https=) |
| DK (1) | DK2545189T3 (https=) |
| ES (1) | ES2665500T3 (https=) |
| WO (1) | WO2011112534A1 (https=) |
Families Citing this family (47)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102859003B (zh) | 2010-03-08 | 2014-07-30 | 达纳-法伯癌症研究院有限公司 | 使用参考封闭序列的全cold-pcr富集 |
| AU2011319755B2 (en) | 2010-10-27 | 2017-02-23 | President And Fellows Of Harvard College | Compositions of toehold primer duplexes and methods of use |
| AU2012223438A1 (en) | 2011-02-28 | 2013-09-26 | Transgenomic, Inc | Kit and method for sequencing a target DNA in a mixed population |
| EP3333269B1 (en) * | 2011-03-31 | 2021-05-05 | Dana-Farber Cancer Institute, Inc. | Methods to enable multiplex cold-pcr |
| US9796748B2 (en) | 2011-05-02 | 2017-10-24 | President And Fellows Of Harvard College | Spatial sequestration of dynamic nucleic acid circuits |
| EP2758458A4 (en) | 2011-07-10 | 2015-10-21 | Harvard College | COMPOSITIONS AND METHODS FOR SELF-ASSEMBLING POLYMERS WITH COMPLEMENTARY MACROSCOPIC AND MICROSCOPIC SCALE UNITS |
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| EP2545189A1 (en) | 2013-01-16 |
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| JP5795341B2 (ja) | 2015-10-14 |
| KR20120139780A (ko) | 2012-12-27 |
| AU2011224534A1 (en) | 2012-09-27 |
| US9957556B2 (en) | 2018-05-01 |
| US20180282798A1 (en) | 2018-10-04 |
| ES2665500T3 (es) | 2018-04-26 |
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