ES2665500T3 - Enriquecimiento de una PCR por COLD completa con secuencia de bloqueo de referencia - Google Patents

Enriquecimiento de una PCR por COLD completa con secuencia de bloqueo de referencia Download PDF

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Publication number
ES2665500T3
ES2665500T3 ES11709836.8T ES11709836T ES2665500T3 ES 2665500 T3 ES2665500 T3 ES 2665500T3 ES 11709836 T ES11709836 T ES 11709836T ES 2665500 T3 ES2665500 T3 ES 2665500T3
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sequence
target
reaction mixture
temperature
chains
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Spanish (es)
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Gerassimos Makrigiorgos
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Dana Farber Cancer Institute Inc
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Dana Farber Cancer Institute Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
ES11709836.8T 2010-03-08 2011-03-08 Enriquecimiento de una PCR por COLD completa con secuencia de bloqueo de referencia Active ES2665500T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US31164210P 2010-03-08 2010-03-08
US311642P 2010-03-08
PCT/US2011/027473 WO2011112534A1 (en) 2010-03-08 2011-03-08 Full cold-pcr enrichment with reference blocking sequence

Publications (1)

Publication Number Publication Date
ES2665500T3 true ES2665500T3 (es) 2018-04-26

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US (3) US8623603B2 (https=)
EP (1) EP2545189B1 (https=)
JP (1) JP5795341B2 (https=)
KR (2) KR101550489B1 (https=)
CN (1) CN102859003B (https=)
AU (1) AU2011224534B2 (https=)
CA (1) CA2792433C (https=)
DK (1) DK2545189T3 (https=)
ES (1) ES2665500T3 (https=)
WO (1) WO2011112534A1 (https=)

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CN102859003B (zh) 2014-07-30
AU2011224534B2 (en) 2014-10-09
CN102859003A (zh) 2013-01-02
US8623603B2 (en) 2014-01-07
WO2011112534A1 (en) 2011-09-15
US11174510B2 (en) 2021-11-16
US20110217714A1 (en) 2011-09-08
CA2792433C (en) 2016-10-11
US20140106362A1 (en) 2014-04-17
KR101550489B1 (ko) 2015-09-07
EP2545189A1 (en) 2013-01-16
JP2013521776A (ja) 2013-06-13
CA2792433A1 (en) 2011-09-15
KR20150067398A (ko) 2015-06-17
EP2545189B1 (en) 2018-01-10
JP5795341B2 (ja) 2015-10-14
KR20120139780A (ko) 2012-12-27
AU2011224534A1 (en) 2012-09-27
US9957556B2 (en) 2018-05-01
US20180282798A1 (en) 2018-10-04
DK2545189T3 (en) 2018-04-23

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