JP2012522736A5 - - Google Patents

Info

Publication number

JP2012522736A5

JP2012522736A5 JP2012502514A JP2012502514A JP2012522736A5 JP 2012522736 A5 JP2012522736 A5 JP 2012522736A5 JP 2012502514 A JP2012502514 A JP 2012502514A JP 2012502514 A JP2012502514 A JP 2012502514A JP 2012522736 A5 JP2012522736 A5 JP 2012522736A5

Authority

JP

Japan

Prior art keywords

chromatography

anion exchange

exchange chromatography

fsh

hydrophobic interaction

Prior art date

2010-04-01

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
• Global Dossier
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• 238000000034 method Methods 0.000 claims 23
• 238000005571 anion exchange chromatography Methods 0.000 claims 13
• 102000012673 Follicle Stimulating Hormone Human genes 0.000 claims 12
• 108010079345 Follicle Stimulating Hormone Proteins 0.000 claims 12
• 229940028334 follicle stimulating hormone Drugs 0.000 claims 12
• 238000001042 affinity chromatography Methods 0.000 claims 8
• 238000004191 hydrophobic interaction chromatography Methods 0.000 claims 7
• 238000005277 cation exchange chromatography Methods 0.000 claims 5
• 239000008194 pharmaceutical composition Substances 0.000 claims 4
• 239000011347 resin Substances 0.000 claims 4
• 229920005989 resin Polymers 0.000 claims 4
• 238000001542 size-exclusion chromatography Methods 0.000 claims 4
• QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 claims 2
• 229920000936 Agarose Polymers 0.000 claims 2
• 238000004587 chromatography analysis Methods 0.000 claims 2
• 239000003480 eluent Substances 0.000 claims 2
• 125000000524 functional group Chemical group 0.000 claims 2
• 239000011159 matrix material Substances 0.000 claims 2
• 239000012528 membrane Substances 0.000 claims 2
• 239000012475 sodium chloride buffer Substances 0.000 claims 2
• 239000012609 strong anion exchange resin Substances 0.000 claims 2
• FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 1
• LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 claims 1
• 229920002307 Dextran Polymers 0.000 claims 1
• JQYMGXZJTCOARG-UHFFFAOYSA-N Reactive blue 2 Chemical compound C1=2C(=O)C3=CC=CC=C3C(=O)C=2C(N)=C(S(O)(=O)=O)C=C1NC(C=C1S(O)(=O)=O)=CC=C1NC(N=1)=NC(Cl)=NC=1NC1=CC=CC(S(O)(=O)=O)=C1 JQYMGXZJTCOARG-UHFFFAOYSA-N 0.000 claims 1
• 230000002378 acidificating effect Effects 0.000 claims 1
• 239000011324 bead Substances 0.000 claims 1
• 125000000484 butyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 claims 1
• 238000005341 cation exchange Methods 0.000 claims 1
• 150000001768 cations Chemical class 0.000 claims 1
• 238000011210 chromatographic step Methods 0.000 claims 1
• 208000021267 infertility disease Diseases 0.000 claims 1
• 239000003446 ligand Substances 0.000 claims 1
• 239000007788 liquid Substances 0.000 claims 1
• 229910021645 metal ion Inorganic materials 0.000 claims 1
• 238000001728 nano-filtration Methods 0.000 claims 1
• 239000000546 pharmaceutical excipient Substances 0.000 claims 1
• 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 claims 1
• 238000004366 reverse phase liquid chromatography Methods 0.000 claims 1
• 238000000108 ultra-filtration Methods 0.000 claims 1
• 238000004271 weak anion exchange chromatography Methods 0.000 claims 1