KR101761168B1 - 재조합 ｆｓｈ 정제 방법 - Google Patents

재조합 ｆｓｈ 정제 방법 Download PDF

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Publication number
KR101761168B1
KR101761168B1 KR1020117025974A KR20117025974A KR101761168B1 KR 101761168 B1 KR101761168 B1 KR 101761168B1 KR 1020117025974 A KR1020117025974 A KR 1020117025974A KR 20117025974 A KR20117025974 A KR 20117025974A KR 101761168 B1 KR101761168 B1 KR 101761168B1
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KR
South Korea
Prior art keywords
fsh
chromatography
way
anion exchange
exchange chromatography
Prior art date
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Expired - Fee Related
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KR1020117025974A
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English (en)
Korean (ko)
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KR20120013359A (ko
Inventor
크리스티안 쉐커만
디트마르 아이킨거
슈테판 아놀트
Original Assignee
바이오제너릭스 게엠베하
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/59—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g.hCG [human chorionic gonadotropin]; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
    • A61P15/08—Drugs for genital or sexual disorders; Contraceptives for gonadal disorders or for enhancing fertility, e.g. inducers of ovulation or of spermatogenesis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/02—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length in solution
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Endocrinology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Reproductive Health (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Pregnancy & Childbirth (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
KR1020117025974A 2009-04-01 2010-04-01 재조합 ｆｓｈ 정제 방법 Expired - Fee Related KR101761168B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP09157133.1 2009-04-01
EP09157133 2009-04-01
PCT/EP2010/002111 WO2010115586A2 (en) 2009-04-01 2010-04-01 Method for purifying recombinant fsh

Publications (2)

Publication Number Publication Date
KR20120013359A KR20120013359A (ko) 2012-02-14
KR101761168B1 true KR101761168B1 (ko) 2017-07-25

Family

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KR1020117025974A Expired - Fee Related KR101761168B1 (ko) 2009-04-01 2010-04-01 재조합 ｆｓｈ 정제 방법

Country Status (26)

Country Link
US (3) US9096683B2 (enExample)
EP (1) EP2414380B1 (enExample)
JP (2) JP5845170B2 (enExample)
KR (1) KR101761168B1 (enExample)
CN (1) CN102448979B (enExample)
AU (1) AU2010234028B2 (enExample)
BR (1) BRPI1012647B1 (enExample)
CA (1) CA2755927A1 (enExample)
CY (1) CY1116963T1 (enExample)
DK (1) DK2414380T3 (enExample)
EA (1) EA028065B1 (enExample)
ES (1) ES2552055T3 (enExample)
HR (1) HRP20151086T1 (enExample)
HU (1) HUE025959T2 (enExample)
IL (1) IL215234A (enExample)
ME (1) ME02277B (enExample)
MX (1) MX2011010480A (enExample)
NZ (1) NZ595661A (enExample)
PL (1) PL2414380T3 (enExample)
PT (1) PT2414380E (enExample)
RS (1) RS54318B1 (enExample)
SI (1) SI2414380T1 (enExample)
SM (1) SMT201500276B (enExample)
UA (1) UA106369C2 (enExample)
WO (1) WO2010115586A2 (enExample)
ZA (1) ZA201106800B (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021132957A1 (ko) * 2019-12-26 2021-07-01 주식회사 엘지화학 여포 자극 호르몬의 정제 방법
KR102283871B1 (ko) * 2021-03-02 2021-08-02 한국원자력연구원 크로마토그래피를 이용한 무담체 홀뮴-166의 분리 방법, 분리 장치 및 이에 의하여 분리된 무담체 홀뮴-166

Families Citing this family (14)

* Cited by examiner, † Cited by third party
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CA2755927A1 (en) * 2009-04-01 2010-10-14 Biogenerix Ag Method for purifying recombinant fsh
CN103570820B (zh) * 2012-08-06 2015-11-18 齐鲁制药有限公司 一种重组人促卵泡激素的纯化方法
KR101516054B1 (ko) * 2012-11-21 2015-05-06 씨제이헬스케어 주식회사 신규한 지속형 인간 성장호르몬 단량체를 제조하는 방법
CN103059125B (zh) * 2012-12-27 2015-08-12 浙江海正药业股份有限公司 重组人促卵泡激素的纯化方法
US20160024181A1 (en) 2013-03-13 2016-01-28 Moderna Therapeutics, Inc. Long-lived polynucleotide molecules
CN103539861B (zh) * 2013-11-01 2015-02-18 广州联康生物科技有限公司 长效重组人促卵泡激素融合蛋白
CN103539860B (zh) * 2013-11-01 2014-12-03 广州优联康医药科技有限公司 一种长效重组人促卵泡激素融合蛋白
AR100131A1 (es) * 2014-04-18 2016-09-14 Cadila Healthcare Ltd Proceso de purificación de gonadotropina
CN104479005B (zh) * 2014-09-05 2017-10-31 上海丽珠制药有限公司 一种促性腺激素纯化的方法
CN105461799A (zh) * 2015-12-31 2016-04-06 哈药集团技术中心 一种重组人促卵泡生成素的纯化方法
CN106117342B (zh) * 2016-07-07 2020-01-03 江西浩然生物医药有限公司 一种高纯度尿促卵泡素的制备方法
RU2694598C1 (ru) * 2018-08-07 2019-07-16 Федеральное государственное бюджетное учреждение науки институт биоорганической химии им. академиков М.М. Шемякина и Ю.А. Овчинникова Российской академии наук (ИБХ РАН) Клеточная линия huFSHKKc6 - продуцент рекомбинантного человеческого фолликулостимулирующего гормона (ФСГ) и способ получения ФСГ с использованием данной линии
CN113372433B (zh) * 2021-03-12 2022-10-11 上海景泽生物技术有限公司 纯化fsh方法
CN114591414A (zh) * 2022-03-24 2022-06-07 江西浩然生物制药有限公司 一种人绒毛膜促性腺激素的制备方法

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US20030059898A1 (en) * 1983-11-02 2003-03-27 Genzyme Corporation Cells, vectors and methods for producing biologically active heterodimeric proteins
WO2007065918A2 (en) * 2005-12-09 2007-06-14 Ares Trading S.A. Method for purifying fsh or a fsh mutant

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US4840896A (en) 1983-11-02 1989-06-20 Integrated Genetics, Inc. Heteropolymeric protein
US4923805A (en) 1983-11-02 1990-05-08 Integrated Genetics, Inc. Fsh
IT1206302B (it) 1987-06-26 1989-04-14 Serono Cesare Ist Ricerca Ormone follicolo-stimolante urinario
CA2018676C (en) 1989-06-20 2003-05-06 Christie A. Kelton Novel heteropolymeric protein production methods
US5021160A (en) * 1990-01-09 1991-06-04 Gelman Sciences, Inc. Strongly acidic microporous membranes for cation exchange
DE4440587A1 (de) 1994-11-14 1996-05-15 Fliether Karl Gmbh & Co Basquill-Schloß
US6261823B1 (en) * 1996-12-13 2001-07-17 Schering Corporation Methods for purifying viruses
PT996629E (pt) 1997-06-25 2006-12-29 Applied Research Systems Análogos de hormonas glicoproteicas reticulados com ligações dissulfito, sua preparação e utilização
US5990288A (en) * 1997-10-21 1999-11-23 Vitro Diagnostics, Inc. Method for purifying FSH
US6414123B1 (en) * 1997-10-21 2002-07-02 Vitro Diagnostics, Inc. Method for purifying FSH
EP1169349B1 (en) 1999-04-16 2007-03-28 Instituto Massone S.A. Process for preparing gonadotropin compositions
WO2001058493A1 (en) 2000-02-11 2001-08-16 Maxygen Aps Conjugates of follicle stimulating hormones
US7157277B2 (en) * 2001-11-28 2007-01-02 Neose Technologies, Inc. Factor VIII remodeling and glycoconjugation of Factor VIII
CA2483650A1 (en) 2002-05-31 2003-12-11 E.I. Du Pont De Nemours And Company Copolymers having tunable energy levels and color of emission
PT1610822E (pt) 2003-04-02 2011-01-05 Ares Trading Sa Formulações farmacêuticas líquidas ou liofilizadas de fsh e/ou lh em conjunto com o surfactante não iónico poloxâmero 188 e um agente bacteriostático
ES2333128T3 (es) * 2003-12-22 2010-02-17 Ares Trading S.A. Metodo para purificar fsh.
SI1809663T1 (sl) * 2004-11-09 2009-02-28 Ares Trading Sa Postopek za äśiĺ äśenje fsh
ES2572629T3 (es) * 2005-12-09 2016-06-01 Ares Trading S.A. Método para purificar FSH o un mutante de FSH
PT2170942E (pt) * 2007-06-28 2013-10-22 Biogenerix Ag Clone celular produtor de fsh
AR067536A1 (es) * 2007-07-17 2009-10-14 Hoffmann La Roche Metodo para obtener una eritropoyetina mono-pegilada en una forma sustancialmente homogenea
CA2755927A1 (en) * 2009-04-01 2010-10-14 Biogenerix Ag Method for purifying recombinant fsh

Patent Citations (2)

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Publication number Priority date Publication date Assignee Title
US20030059898A1 (en) * 1983-11-02 2003-03-27 Genzyme Corporation Cells, vectors and methods for producing biologically active heterodimeric proteins
WO2007065918A2 (en) * 2005-12-09 2007-06-14 Ares Trading S.A. Method for purifying fsh or a fsh mutant

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021132957A1 (ko) * 2019-12-26 2021-07-01 주식회사 엘지화학 여포 자극 호르몬의 정제 방법
WO2021132958A1 (ko) * 2019-12-26 2021-07-01 주식회사 엘지화학 여포 자극 호르몬의 정제 방법
KR102283871B1 (ko) * 2021-03-02 2021-08-02 한국원자력연구원 크로마토그래피를 이용한 무담체 홀뮴-166의 분리 방법, 분리 장치 및 이에 의하여 분리된 무담체 홀뮴-166

Also Published As

Publication number Publication date
HRP20151086T1 (hr) 2015-12-04
EP2414380A2 (en) 2012-02-08
DK2414380T3 (en) 2015-10-19
EA028065B1 (ru) 2017-10-31
JP5845170B2 (ja) 2016-01-20
JP6231056B2 (ja) 2017-11-15
HUE025959T2 (en) 2016-05-30
BRPI1012647A2 (pt) 2020-08-11
IL215234A (en) 2016-09-29
EP2414380B1 (en) 2015-08-26
PL2414380T3 (pl) 2016-01-29
SI2414380T1 (sl) 2015-12-31
CN102448979A (zh) 2012-05-09
AU2010234028B2 (en) 2013-11-14
CN102448979B (zh) 2015-03-04
BRPI1012647B1 (pt) 2022-05-17
CY1116963T1 (el) 2017-04-05
UA106369C2 (ru) 2014-08-26
US20120135928A1 (en) 2012-05-31
EA201171199A1 (ru) 2012-05-30
CA2755927A1 (en) 2010-10-14
ZA201106800B (en) 2012-08-28
ME02277B (me) 2016-02-20
WO2010115586A3 (en) 2010-11-25
PT2414380E (pt) 2015-11-13
US20160304575A1 (en) 2016-10-20
IL215234A0 (en) 2011-12-29
US20150353599A1 (en) 2015-12-10
NZ595661A (en) 2013-07-26
JP2012522736A (ja) 2012-09-27
KR20120013359A (ko) 2012-02-14
JP2016027050A (ja) 2016-02-18
ES2552055T3 (es) 2015-11-25
WO2010115586A2 (en) 2010-10-14
RS54318B1 (sr) 2016-02-29
AU2010234028A1 (en) 2011-10-27
SMT201500276B (it) 2016-01-08
MX2011010480A (es) 2012-09-07
US9096683B2 (en) 2015-08-04

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