UA106369C2 - Способ очистки рекомбинантного фсг - Google Patents

Способ очистки рекомбинантного фсг

Info

Publication number
UA106369C2
UA106369C2 UAA201112796A UAA201112796A UA106369C2 UA 106369 C2 UA106369 C2 UA 106369C2 UA A201112796 A UAA201112796 A UA A201112796A UA A201112796 A UAA201112796 A UA A201112796A UA 106369 C2 UA106369 C2 UA 106369C2
Authority
UA
Ukraine
Prior art keywords
fsh
recombinant
recombinant fsh
chromatography
anion exchange
Prior art date
Application number
UAA201112796A
Other languages
English (en)
Ukrainian (uk)
Inventor
Кристиан Шеккерманн
Дитмар Айхингер
Штефан Арнольд
Original Assignee
Рациофарм Гмбх
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Рациофарм Гмбх filed Critical Рациофарм Гмбх
Publication of UA106369C2 publication Critical patent/UA106369C2/ru

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
    • A61P15/08—Drugs for genital or sexual disorders; Contraceptives for gonadal disorders or for enhancing fertility, e.g. inducers of ovulation or of spermatogenesis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/02—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length in solution
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/59—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g.hCG [human chorionic gonadotropin]; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Endocrinology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Reproductive Health (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Public Health (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Gynecology & Obstetrics (AREA)
  • Pregnancy & Childbirth (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Изобретение относится к способу очистки рекомбинантного фолликулостимулирующего гормона (ФСГ) или варианта рекомбинантного ФСГ. Способ включает стадии, на которых жидкость, содержащая рекомбинантный ФСГ или вариант рекомбинантного ФСГ, подвергают анионообменной хроматографии, хроматографии гидрофобного взаимодействия и хроматографии по сродству к красителю, причем эти хроматографические стадии могут быть выполнены в любом порядке, и причем данный способ не включает ни слабую анионообменную хроматографию, ни хроматографию с обратной фазой. В результате способа очистки получают высокий выход рекомбинантного ФСГ с необходимой мерой чистоты. Полученный ФСГ является чрезвычайно эффективным для профилактики и лечения заболеваний и медицинских показаний, при которых препараты ФСГ считаются эффективным средст�
UAA201112796A 2009-04-01 2010-04-01 Способ очистки рекомбинантного фсг UA106369C2 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP09157133 2009-04-01
PCT/EP2010/002111 WO2010115586A2 (en) 2009-04-01 2010-04-01 Method for purifying recombinant fsh

Publications (1)

Publication Number Publication Date
UA106369C2 true UA106369C2 (ru) 2014-08-26

Family

ID=42153744

Family Applications (1)

Application Number Title Priority Date Filing Date
UAA201112796A UA106369C2 (ru) 2009-04-01 2010-04-01 Способ очистки рекомбинантного фсг

Country Status (26)

Country Link
US (3) US9096683B2 (ru)
EP (1) EP2414380B1 (ru)
JP (2) JP5845170B2 (ru)
KR (1) KR101761168B1 (ru)
CN (1) CN102448979B (ru)
AU (1) AU2010234028B2 (ru)
BR (1) BRPI1012647B1 (ru)
CA (1) CA2755927A1 (ru)
CY (1) CY1116963T1 (ru)
DK (1) DK2414380T3 (ru)
EA (1) EA028065B1 (ru)
ES (1) ES2552055T3 (ru)
HR (1) HRP20151086T1 (ru)
HU (1) HUE025959T2 (ru)
IL (1) IL215234A (ru)
ME (1) ME02277B (ru)
MX (1) MX2011010480A (ru)
NZ (1) NZ595661A (ru)
PL (1) PL2414380T3 (ru)
PT (1) PT2414380E (ru)
RS (1) RS54318B1 (ru)
SI (1) SI2414380T1 (ru)
SM (1) SMT201500276B (ru)
UA (1) UA106369C2 (ru)
WO (1) WO2010115586A2 (ru)
ZA (1) ZA201106800B (ru)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5845170B2 (ja) * 2009-04-01 2016-01-20 ラティオファーム ゲーエムベーハー 組換えｆｓｈを精製する方法
CN103570820B (zh) * 2012-08-06 2015-11-18 齐鲁制药有限公司 一种重组人促卵泡激素的纯化方法
KR101516054B1 (ko) * 2012-11-21 2015-05-06 씨제이헬스케어 주식회사 신규한 지속형 인간 성장호르몬 단량체를 제조하는 방법
CN103059125B (zh) * 2012-12-27 2015-08-12 浙江海正药业股份有限公司 重组人促卵泡激素的纯化方法
US20160024181A1 (en) 2013-03-13 2016-01-28 Moderna Therapeutics, Inc. Long-lived polynucleotide molecules
CN103539860B (zh) * 2013-11-01 2014-12-03 广州优联康医药科技有限公司 一种长效重组人促卵泡激素融合蛋白
CN103539861B (zh) * 2013-11-01 2015-02-18 广州联康生物科技有限公司 长效重组人促卵泡激素融合蛋白
SG11201608513WA (en) * 2014-04-18 2016-11-29 Cadila Healthcare Ltd Novel purification process of gonadotropin
CN104479005B (zh) * 2014-09-05 2017-10-31 上海丽珠制药有限公司 一种促性腺激素纯化的方法
CN105461799A (zh) * 2015-12-31 2016-04-06 哈药集团技术中心 一种重组人促卵泡生成素的纯化方法
CN106117342B (zh) * 2016-07-07 2020-01-03 江西浩然生物医药有限公司 一种高纯度尿促卵泡素的制备方法
RU2694598C1 (ru) * 2018-08-07 2019-07-16 Федеральное государственное бюджетное учреждение науки институт биоорганической химии им. академиков М.М. Шемякина и Ю.А. Овчинникова Российской академии наук (ИБХ РАН) Клеточная линия huFSHKKc6 - продуцент рекомбинантного человеческого фолликулостимулирующего гормона (ФСГ) и способ получения ФСГ с использованием данной линии
WO2021132958A1 (ko) * 2019-12-26 2021-07-01 주식회사 엘지화학 여포 자극 호르몬의 정제 방법
KR102283871B1 (ko) * 2021-03-02 2021-08-02 한국원자력연구원 크로마토그래피를 이용한 무담체 홀뮴-166의 분리 방법, 분리 장치 및 이에 의하여 분리된 무담체 홀뮴-166
CN113372433B (zh) * 2021-03-12 2022-10-11 上海景泽生物技术有限公司 纯化fsh方法
CN114591414A (zh) * 2022-03-24 2022-06-07 江西浩然生物制药有限公司 一种人绒毛膜促性腺激素的制备方法

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4840896A (en) * 1983-11-02 1989-06-20 Integrated Genetics, Inc. Heteropolymeric protein
US6455282B1 (en) * 1983-11-02 2002-09-24 Genzyme Corporation Cells, vectors and methods for producing biologically active TSH
US4923805A (en) 1983-11-02 1990-05-08 Integrated Genetics, Inc. Fsh
IT1206302B (it) 1987-06-26 1989-04-14 Serono Cesare Ist Ricerca Ormone follicolo-stimolante urinario
CA2018676C (en) 1989-06-20 2003-05-06 Christie A. Kelton Novel heteropolymeric protein production methods
US5021160A (en) * 1990-01-09 1991-06-04 Gelman Sciences, Inc. Strongly acidic microporous membranes for cation exchange
DE4440587A1 (de) 1994-11-14 1996-05-15 Fliether Karl Gmbh & Co Basquill-Schloß
US6261823B1 (en) * 1996-12-13 2001-07-17 Schering Corporation Methods for purifying viruses
IL133686A0 (en) 1997-06-25 2001-04-30 Applied Research Systems Glycoprotein hormone analogs, their preparation and use
US6414123B1 (en) * 1997-10-21 2002-07-02 Vitro Diagnostics, Inc. Method for purifying FSH
US5990288A (en) * 1997-10-21 1999-11-23 Vitro Diagnostics, Inc. Method for purifying FSH
DK1169349T3 (da) * 1999-04-16 2007-07-30 Inst Massone S A Proces til fremstilling af gonadotropinsammensætninger
WO2001058493A1 (en) 2000-02-11 2001-08-16 Maxygen Aps Conjugates of follicle stimulating hormones
US7157277B2 (en) * 2001-11-28 2007-01-02 Neose Technologies, Inc. Factor VIII remodeling and glycoconjugation of Factor VIII
CA2483650A1 (en) 2002-05-31 2003-12-11 E.I. Du Pont De Nemours And Company Copolymers having tunable energy levels and color of emission
EA012565B1 (ru) 2003-04-02 2009-10-30 Арес Трейдинг С.А. Жидкие фармацевтические композиции фолликулостимулирующего гормона (фсг) и лютеинизирующего гормона (лг), содержащие неионогенное поверхностно-активное вещество
EA008924B1 (ru) * 2003-12-22 2007-08-31 Арес Трейдинг С.А. Способ очистки фсг
EP1809663B1 (en) * 2004-11-09 2008-09-17 Ares Trading S.A. Method for purifying fsh
WO2007065918A2 (en) * 2005-12-09 2007-06-14 Ares Trading S.A. Method for purifying fsh or a fsh mutant
TW200728316A (en) * 2005-12-09 2007-08-01 Ares Trading Sa Method for purifying FSH or a FSH mutant
AU2008267138B9 (en) 2007-06-28 2014-06-05 Theramex HQ UK Limited FSH producing cell clone
CL2008002054A1 (es) * 2007-07-17 2009-05-29 Hoffmann La Roche Metodo para la regeneracion de una columna de cromatografia de intercambio cationico despues de la elusion de eritropoyetina monopeguilada y metodo para obtener una eritropoyetina monopeguilada, incorporando el metodo de regeneracion de la columna de intercambio cationico.
JP5845170B2 (ja) * 2009-04-01 2016-01-20 ラティオファーム ゲーエムベーハー 組換えｆｓｈを精製する方法

Also Published As

Publication number Publication date
BRPI1012647B1 (pt) 2022-05-17
CY1116963T1 (el) 2017-04-05
EP2414380A2 (en) 2012-02-08
HRP20151086T1 (hr) 2015-12-04
SMT201500276B (it) 2016-01-08
US20120135928A1 (en) 2012-05-31
IL215234A0 (en) 2011-12-29
JP2016027050A (ja) 2016-02-18
IL215234A (en) 2016-09-29
CA2755927A1 (en) 2010-10-14
EA201171199A1 (ru) 2012-05-30
JP5845170B2 (ja) 2016-01-20
AU2010234028A1 (en) 2011-10-27
WO2010115586A3 (en) 2010-11-25
RS54318B1 (en) 2016-02-29
SI2414380T1 (sl) 2015-12-31
WO2010115586A2 (en) 2010-10-14
DK2414380T3 (en) 2015-10-19
JP2012522736A (ja) 2012-09-27
HUE025959T2 (en) 2016-05-30
EA028065B1 (ru) 2017-10-31
BRPI1012647A2 (pt) 2020-08-11
KR101761168B1 (ko) 2017-07-25
CN102448979A (zh) 2012-05-09
CN102448979B (zh) 2015-03-04
MX2011010480A (es) 2012-09-07
ES2552055T3 (es) 2015-11-25
NZ595661A (en) 2013-07-26
AU2010234028B2 (en) 2013-11-14
US20160304575A1 (en) 2016-10-20
ME02277B (me) 2016-02-20
US20150353599A1 (en) 2015-12-10
PL2414380T3 (pl) 2016-01-29
PT2414380E (pt) 2015-11-13
JP6231056B2 (ja) 2017-11-15
US9096683B2 (en) 2015-08-04
EP2414380B1 (en) 2015-08-26
KR20120013359A (ko) 2012-02-14
ZA201106800B (en) 2012-08-28

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