JP2012512902A - 種子のコーティング中のパスツーリア菌胞子によって線虫を防除するための材料および方法 - Google Patents
種子のコーティング中のパスツーリア菌胞子によって線虫を防除するための材料および方法 Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
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- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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Abstract
Description
本出願は、全ての図、表または図面を含むその全体を参照により本明細書に組み入れられる、2008年12月19日に出願された米国特許仮出願第61/139,304号の恩典を主張する。
植物寄生線虫による作物損失は千億ドルを超過すると見積もられている。この被害を予防することは重要な課題となっている。燻蒸剤臭化メチルの使用廃止が差し迫る中、線虫の防除のための新規の合成化合物を開発し登録するには十分な時間がない。したがって、他の選択肢が必要とされている。
本発明は、種子に付着された有効量のパスツーリア菌胞子を含む組成物を土壌に送達することにより、植物病原性線虫および/または他の土壌線虫を防除するための、新規のかつ好都合な材料および方法を提供する。
本発明は、パスツーリア菌胞子でコーティングした植物の種子の利用による、植物病原性線虫および/または他の土壌線虫の効率よい防除のための材料および方法を提供する。
本発明のパスツーリア菌は、製剤化されていない胞子として、または、製剤化された液体あるいは固体の組成物、粒子のスラリー、もしくは乳濁液として種子に送達することができる。
本発明の材料および方法は、非限定的にサヤマメ、芝草、トマト、綿、トウモロコシ、ダイズ、野菜、コムギ、オオムギ、イネおよびキャノーラを含む植物種の被害を軽減するために使用できる。
本発明の材料および方法は、線虫を殺滅、防除、および/もしくは感染させるために;線虫の成長もしくは生殖を遅らせるために;線虫集団を減少させるために;ならびに/または、非限定的にアレナリアネコブセンチュウ(Meloidogyne arenaria)、ブラキュルスネグサレセンチュウ(Pratylenchus brachyurus)、ニセフクロセンチュウ(Rotylenchulus reniformis)、およびベロノライムス・ロンジカウダタス(Belonolaimus longicaudatus)、ダイズシストセンチュウ(Heterodera glycines)およびホプロライムス・ガレアツス(Hoplolaimus galeatus)を含む、植物病原性線虫、植物寄生線虫、および他の土壌線虫によって生じる植物の被害を軽減する、もしくは遅延させるために有用である。
本発明は、パスツーリア菌組成物を植物の種子に付着させ、該種子を線虫感染の場に送達する方法をも提供する。
本実施例は、パスツーリア・ペネトランス胞子で種子をコーティングする方法を例証する。パスツーリア菌胞子を10 mmol/Lのリン酸緩衝液に懸濁し、約2×107 胞子/mlに調整する。5 mlの胞子懸濁液を2 gのAXIS(登録商標)珪藻土顆粒とペトリ皿にて混合することによって;または、3 mlの胞子懸濁液を2 gのPROFILE(登録商標)greens grade粘土顆粒とペトリ皿にて混合することによってパスツーリア菌-顆粒状混合物を得る。
パスツーリア菌コーティングされた種子の線虫に対する効果を評価するために、温室ポット試験を行う。
Claims (12)
- 有効量のパスツーリア菌(Pasteuria)胞子を含むパスツーリア菌組成物を種子に付着させる段階;および該種子を植付ける段階を含む、植物病原性線虫および/または土壌線虫を防除するための方法。
- パスツーリア菌組成物が、種子に施された場合に約106〜約109 胞子/種子の範囲の濃度で存在する、請求項1記載の方法。
- パスツーリア菌組成物が液体または固体の担体をさらに含む、請求項1記載の方法。
- 固体の担体が乾燥粉末、水和性粉末、水和性顆粒、乾燥顆粒、粒子、高分子マトリクス、およびスラリーからなる群より選択される、請求項3記載の方法。
- 固体の担体が顆粒を含む、請求項4記載の方法。
- 固体の担体がポリアクリルアミド、デンプン、粘土、シリカ、アルミナ、土、砂、ポリ尿素、ポリアクリレート、およびその組み合わせのうち任意のものからなる群より選択される高分子マトリクスである、請求項4記載の方法。
- 接着剤が種子に施され、かつ該接着剤がポリ酢酸ビニル、ポリ酢酸ビニルコポリマー、ポリビニルアルコール、ポリビニルアルコールコポリマー、メチルセルロース、ヒドロキシメチルセルロース、ヒドロキシメチルプロピルセルロース、デキストリン、アルジネート、糖蜜、ポリビニルピロリドン、多糖、タンパク質、脂肪、油、多糖、アラビアガム、ゼラチン、シロップ、およびその任意の組み合わせからなる群より選択される、請求項1記載の方法。
- パスツーリア菌胞子がパスツーリア・ペネトランス(Pasteuria penetrans)、パスツーリア・ラモサ(Pasteuria ramose)、パスツーリア・ソルネイ(Pasteuria thornei)、パスツーリア・ニシザワエ(Pasteuria nishizawae)、およびその任意の組み合わせからなる群より選択される、請求項1記載の方法。
- 植物病原性線虫および/または土壌線虫がアレナリアネコブセンチュウ(Meloidogyne arenaria)、ブラキュルスネグサレセンチュウ(Pratylenchus brachyurus)、ニセフクロセンチュウ(Rotylenchulus reniformis)、ベロノライムス・ロンジカウダタス(Belonolaimus longicaudatus)、およびその任意の組み合わせからなる群より選択される、請求項1記載の方法。
- 種子が、サヤマメ、トマト、綿、トウモロコシ、ダイズ、コムギ、オオムギ、イネ、キャノーラの種子、およびその任意の組み合わせからなる群より選択される、請求項1記載の方法。
- 種子が、殺虫剤、除草剤、殺菌剤、肥料、および生物防除剤からなる群より選択される第二の農業的に有益な成分をさらに含む、請求項1記載の方法。
- 線虫の防除のために有効な量のパスツーリア菌胞子を含むパスツーリア菌組成物で表面積の少なくとも一部をコーティングされた種子。
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Application Number | Priority Date | Filing Date | Title |
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US13930408P | 2008-12-19 | 2008-12-19 | |
US61/139,304 | 2008-12-19 | ||
PCT/US2009/068732 WO2010080619A2 (en) | 2008-12-19 | 2009-12-18 | Materials and methods for controlling nematodes with pasteuria spores in seed coatings |
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JP2012512902A true JP2012512902A (ja) | 2012-06-07 |
JP5654481B2 JP5654481B2 (ja) | 2015-01-14 |
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US (4) | US8598082B2 (ja) |
EP (1) | EP2373148B1 (ja) |
JP (1) | JP5654481B2 (ja) |
KR (2) | KR101708422B1 (ja) |
CN (1) | CN102316715B (ja) |
AR (1) | AR075492A1 (ja) |
AU (1) | AU2009335725B8 (ja) |
BR (1) | BRPI0922998B1 (ja) |
CA (2) | CA2950287C (ja) |
DK (1) | DK2373148T3 (ja) |
EA (3) | EA201991236A1 (ja) |
IL (1) | IL213201A (ja) |
MX (1) | MX2011006546A (ja) |
PL (1) | PL2373148T3 (ja) |
UA (1) | UA108065C2 (ja) |
WO (1) | WO2010080619A2 (ja) |
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