JP2012511927A5 - - Google Patents
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- JP2012511927A5 JP2012511927A5 JP2011542142A JP2011542142A JP2012511927A5 JP 2012511927 A5 JP2012511927 A5 JP 2012511927A5 JP 2011542142 A JP2011542142 A JP 2011542142A JP 2011542142 A JP2011542142 A JP 2011542142A JP 2012511927 A5 JP2012511927 A5 JP 2012511927A5
- Authority
- JP
- Japan
- Prior art keywords
- allele
- specific
- specific primer
- reaction mixture
- kit
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 108700028369 Alleles Proteins 0.000 claims description 254
- 239000000523 sample Substances 0.000 claims description 176
- 239000011541 reaction mixture Substances 0.000 claims description 74
- 239000002773 nucleotide Substances 0.000 claims description 68
- 125000003729 nucleotide group Chemical group 0.000 claims description 68
- 238000000034 method Methods 0.000 claims description 66
- 239000000203 mixture Substances 0.000 claims description 60
- 108020004707 nucleic acids Proteins 0.000 claims description 40
- 150000007523 nucleic acids Chemical class 0.000 claims description 40
- 102000039446 nucleic acids Human genes 0.000 claims description 40
- 238000001514 detection method Methods 0.000 claims description 32
- 230000000295 complement effect Effects 0.000 claims description 30
- 206010028980 Neoplasm Diseases 0.000 claims description 24
- 230000035772 mutation Effects 0.000 claims description 24
- 238000011895 specific detection Methods 0.000 claims description 22
- 108020004705 Codon Proteins 0.000 claims description 16
- 206010069755 K-ras gene mutation Diseases 0.000 claims description 16
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 claims description 16
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 claims description 16
- 102000016914 ras Proteins Human genes 0.000 claims description 16
- 108010014186 ras Proteins Proteins 0.000 claims description 16
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 claims description 16
- 239000011230 binding agent Substances 0.000 claims description 10
- 230000003321 amplification Effects 0.000 claims description 9
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 9
- 229930024421 Adenine Natural products 0.000 claims description 8
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 claims description 8
- 108091093088 Amplicon Proteins 0.000 claims description 8
- 102100039788 GTPase NRas Human genes 0.000 claims description 8
- 101000744505 Homo sapiens GTPase NRas Proteins 0.000 claims description 8
- 101710163270 Nuclease Proteins 0.000 claims description 8
- 229960000643 adenine Drugs 0.000 claims description 8
- 239000000872 buffer Substances 0.000 claims description 8
- 239000003153 chemical reaction reagent Substances 0.000 claims description 8
- 229940104302 cytosine Drugs 0.000 claims description 8
- 102000052116 epidermal growth factor receptor activity proteins Human genes 0.000 claims description 8
- 108700015053 epidermal growth factor receptor activity proteins Proteins 0.000 claims description 8
- YOHYSYJDKVYCJI-UHFFFAOYSA-N n-[3-[[6-[3-(trifluoromethyl)anilino]pyrimidin-4-yl]amino]phenyl]cyclopropanecarboxamide Chemical compound FC(F)(F)C1=CC=CC(NC=2N=CN=C(NC=3C=C(NC(=O)C4CC4)C=CC=3)C=2)=C1 YOHYSYJDKVYCJI-UHFFFAOYSA-N 0.000 claims description 8
- 229940113082 thymine Drugs 0.000 claims description 8
- 206010006187 Breast cancer Diseases 0.000 claims description 6
- 208000026310 Breast neoplasm Diseases 0.000 claims description 6
- 208000005443 Circulating Neoplastic Cells Diseases 0.000 claims description 6
- 108020004414 DNA Proteins 0.000 claims description 6
- 206010058467 Lung neoplasm malignant Diseases 0.000 claims description 6
- 239000008280 blood Substances 0.000 claims description 6
- 210000004369 blood Anatomy 0.000 claims description 6
- 201000005202 lung cancer Diseases 0.000 claims description 6
- 208000020816 lung neoplasm Diseases 0.000 claims description 6
- 238000003752 polymerase chain reaction Methods 0.000 claims description 6
- 108090000623 proteins and genes Proteins 0.000 claims description 6
- 238000003753 real-time PCR Methods 0.000 claims description 4
- 238000002844 melting Methods 0.000 claims 4
- 230000008018 melting Effects 0.000 claims 4
- 238000012408 PCR amplification Methods 0.000 description 1
Applications Claiming Priority (13)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US13852108P | 2008-12-17 | 2008-12-17 | |
| US61/138,521 | 2008-12-17 | ||
| US16423009P | 2009-03-27 | 2009-03-27 | |
| US61/164,230 | 2009-03-27 | ||
| US18677509P | 2009-06-12 | 2009-06-12 | |
| US61/186,775 | 2009-06-12 | ||
| US25162309P | 2009-10-14 | 2009-10-14 | |
| US61/251,623 | 2009-10-14 | ||
| US25350109P | 2009-10-20 | 2009-10-20 | |
| US61/253,501 | 2009-10-20 | ||
| US25858209P | 2009-11-05 | 2009-11-05 | |
| US61/258,582 | 2009-11-05 | ||
| PCT/US2009/006652 WO2010077324A2 (en) | 2008-12-17 | 2009-12-17 | Methods, compositions, and kits for detecting allelic variants |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2012511927A JP2012511927A (ja) | 2012-05-31 |
| JP2012511927A5 true JP2012511927A5 (enExample) | 2013-08-15 |
Family
ID=42310453
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011542142A Pending JP2012511927A (ja) | 2008-12-17 | 2009-12-17 | 対立遺伝子変種を検出するための方法、組成物、およびキット |
Country Status (6)
| Country | Link |
|---|---|
| US (6) | US9534255B2 (enExample) |
| EP (1) | EP2376659B1 (enExample) |
| JP (1) | JP2012511927A (enExample) |
| CN (1) | CN102301005A (enExample) |
| CA (1) | CA2747026A1 (enExample) |
| WO (2) | WO2010077324A2 (enExample) |
Families Citing this family (39)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3216874A1 (en) | 2008-09-05 | 2017-09-13 | TOMA Biosciences, Inc. | Methods for stratifying and annotating cancer drug treatment options |
| JP2012511927A (ja) | 2008-12-17 | 2012-05-31 | ライフ テクノロジーズ コーポレーション | 対立遺伝子変種を検出するための方法、組成物、およびキット |
| US8606527B2 (en) * | 2009-02-27 | 2013-12-10 | Bio-Rad Laboratories, Inc. | SNP detection by melt curve clustering |
| EP3249053A1 (en) | 2009-03-27 | 2017-11-29 | Life Technologies Corporation | Methods, compositions, and kits for detecting allelic variants |
| EP2494066B1 (en) * | 2009-10-27 | 2017-04-05 | Swift Biosciences, Inc. | Bimolecular primers |
| CN102115782B (zh) * | 2009-12-30 | 2014-06-18 | 北京雅康博生物科技有限公司 | 用于定量检测K-ras突变的试剂盒 |
| US20130071844A1 (en) * | 2010-03-24 | 2013-03-21 | Toppan Printing Co., Ltd. | Method for detecting target base sequence using competitive primer |
| KR20130113447A (ko) | 2010-09-24 | 2013-10-15 | 더 보드 어브 트러스티스 어브 더 리랜드 스탠포드 주니어 유니버시티 | 고정된 프라이머들을 이용하여 표적 dna의 직접적인 캡쳐, 증폭 및 서열화 |
| CN103459612A (zh) * | 2010-12-03 | 2013-12-18 | 布兰代斯大学 | 用于检测野生型群体中的核酸突变体的方法和试剂盒 |
| GB201100150D0 (en) * | 2011-01-06 | 2011-02-23 | Epistem Ltd | Mutational analysis |
| US9528157B2 (en) * | 2011-01-14 | 2016-12-27 | Genefirst Limited | Methods, compositions, and kits for determing the presence/absence of a variant nucleic acid sequence |
| EP2663654A1 (en) * | 2011-01-14 | 2013-11-20 | Life Technologies Corporation | Methods, compositions, and kits for detecting rare cells |
| WO2012151560A2 (en) | 2011-05-04 | 2012-11-08 | Biocept, Inc. | Methods for detecting nucleic acid sequence variants |
| CN102367478B (zh) * | 2011-10-25 | 2013-07-31 | 浙江大学 | 用于KRAS基因突变分型的ARMS-qPCR检测试剂盒及检测方法 |
| US9738935B2 (en) * | 2011-11-10 | 2017-08-22 | Roche Molecular Systems, Inc. | Complex mutations in the epidermal growth factor receptor kinase domain |
| EP4361607A3 (en) | 2012-02-03 | 2024-07-17 | California Institute of Technology | Signal encoding and decoding in multiplexed biochemical assays |
| JP6108407B2 (ja) * | 2012-03-15 | 2017-04-05 | 公益財団法人ヒューマンサイエンス振興財団 | マクロライド系抗生物質耐性変異菌の検出方法及び検出キット |
| EP2825313B1 (en) | 2012-03-16 | 2018-05-16 | Life Technologies Corporation | Systems and methods for assessing of biological samples |
| CN102618646B (zh) * | 2012-03-30 | 2014-08-20 | 南方医科大学 | 一种基因拷贝数的定量检测方法 |
| EP2880184B1 (en) | 2012-08-03 | 2021-03-31 | California Institute of Technology | Multiplexing and quantification in pcr with reduced hardware and requirements |
| EP2749654A1 (en) * | 2012-12-28 | 2014-07-02 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Method of analysis of composition of nucleic acid mixtures |
| ES2662825T3 (es) * | 2013-02-25 | 2018-04-09 | Seegene, Inc. | Detección de variación de nucleótido en una secuencia de ácidos nucleicos diana |
| KR20160106041A (ko) * | 2013-08-14 | 2016-09-09 | 퀴아젠 맨스필드, 인코퍼레이티드 | Ｎｒａｓ 및 ｂｒａｆ 핵산의 멀티플렉스 분석을 위한 조성물 및 방법 |
| CA2939124C (en) | 2014-02-07 | 2023-08-29 | University Of Iowa Research Foundation | Oligonucleotide-based probes and methods for detection of microbes |
| CN104232750A (zh) * | 2014-05-30 | 2014-12-24 | 嘉兴雅康博医学检验所有限公司 | Nras基因突变检测试剂盒 |
| US10329605B2 (en) * | 2015-04-20 | 2019-06-25 | Neogenomics Laboratories, Inc. | Method to increase sensitivity of detection of low-occurrence mutations |
| CN104762410A (zh) * | 2015-04-29 | 2015-07-08 | 上海允英医疗科技有限公司 | 用于全ras突变检测的引物、探针及检测试剂盒 |
| CN104862401B (zh) * | 2015-05-29 | 2017-10-31 | 沈阳优吉诺生物科技有限公司 | 检测kras基因热点突变位点的引物、试剂盒及其pcr方法 |
| US9657353B2 (en) | 2015-06-26 | 2017-05-23 | Great Basin Scientific, Inc. | Specific detection of organisms derived from a sample |
| CN106520919A (zh) * | 2016-09-30 | 2017-03-22 | 苏州新海生物科技股份有限公司 | 一种检测目标核酸序列变异体的组合物和方法及试剂盒 |
| CN107022607B (zh) * | 2017-03-16 | 2019-01-22 | 北京博奥晶典生物技术有限公司 | 通过碱基错配解决多重pcr时等位基因扩增不平衡的方法 |
| JP7722675B2 (ja) * | 2017-05-16 | 2025-08-13 | 公立大学法人和歌山県立医科大学 | Ｅｇｆｒ変異非小細胞肺がんにおけるｅｇｆｒチロシンキナーゼ阻害剤の治療奏功性の予測方法 |
| CN107577921A (zh) * | 2017-08-25 | 2018-01-12 | 云壹生物技术（大连）有限公司 | 一种肿瘤靶向基因测序数据解析方法 |
| JP7421202B2 (ja) | 2017-09-29 | 2024-01-24 | ユニバーシティー オブ アイオワ リサーチ ファンデーション | デジタルヌクレアーゼ検出組成物および方法 |
| EP3781710A4 (en) | 2018-04-17 | 2022-01-05 | Chromacode, Inc. | METHODS AND SYSTEMS FOR MULTIPLEX ANALYSIS |
| CN110438223B (zh) * | 2018-05-03 | 2023-03-14 | 同济大学苏州研究院 | 检测Kras基因点突变的引物、探针及其试剂盒与检测方法 |
| US12203129B2 (en) | 2018-07-03 | 2025-01-21 | ChromaCode, Inc. | Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays |
| US20210189473A1 (en) * | 2018-09-06 | 2021-06-24 | ChromaCode,Inc. | Universal tail primers with multiple binding motifs for multiplexed detection of single nucleotide polymorphisms |
| CN119287016A (zh) * | 2023-02-01 | 2025-01-10 | 珠海圣美生物诊断技术有限公司 | 多重数字pcr检测试剂盒及其检测方法 |
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-
2009
- 2009-12-17 JP JP2011542142A patent/JP2012511927A/ja active Pending
- 2009-12-17 CN CN2009801557753A patent/CN102301005A/zh active Pending
- 2009-12-17 WO PCT/US2009/006652 patent/WO2010077324A2/en not_active Ceased
- 2009-12-17 US US12/641,321 patent/US9534255B2/en active Active
- 2009-12-17 CA CA2747026A patent/CA2747026A1/en not_active Withdrawn
- 2009-12-17 EP EP09836510.9A patent/EP2376659B1/en not_active Not-in-force
- 2009-12-18 WO PCT/US2009/068610 patent/WO2010080559A2/en not_active Ceased
-
2015
- 2015-10-09 US US14/879,156 patent/US20160040256A1/en not_active Abandoned
-
2018
- 2018-03-16 US US15/923,954 patent/US10570459B2/en active Active
-
2020
- 2020-01-16 US US16/745,296 patent/US11530450B2/en active Active
-
2022
- 2022-12-06 US US18/075,729 patent/US20230323472A1/en active Pending
- 2022-12-22 US US18/087,259 patent/US20240132946A1/en not_active Abandoned
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